Restriction map for
pF1KE0186
(Circular) (Six-base) MAPSORT of: pF1KE0186.seq Check: 7292 from: 1 to: 3773
pF1KE0186 3773 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 811 811
Size: 3773
Acc65I G'GTAC_C
Cuts at: 789 789
Size: 3773
AclI AA'CG_TT
Cuts at: 147 3613 147
Size: 3466 307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1598 2030 2777 3045 502
Size: 1096 432 747 268 1230
Fragments arranged by size:
1230 1096 747 432 268
AflIII A'CryG_T
Cuts at: 233 307 1125 2229 3206 233
Size: 74 818 1104 977 800
Fragments arranged by size:
1104 977 818 800 74
AgeI A'CCGG_T
Cuts at: 2161 2161
Size: 3773
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 746 778 746
Size: 32 3741
AlwNI CAG_nnn'CTG
Cuts at: 1078 2645 1078
Size: 1567 2206
ApaLI G'TGCA_C
Cuts at: 2543 2543
Size: 3773
ApoI r'AATT_y
Cuts at: 160 616 639 777 3126 3382 160
Size: 456 23 138 2349 256 551
Fragments arranged by size:
2349 551 456 256 138 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 3773
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
AvaI C'yCGr_G
Cuts at: 793 793
Size: 3773
BamHI G'GATC_C
Cuts at: 798 798
Size: 3773
BanI G'GyrC_C
Cuts at: 789 1203 1451 1486 789
Size: 414 248 35 3076
Fragments arranged by size:
3076 414 248 35
BanII G_rGCy'C
Cuts at: 787 1817 787
Size: 1030 2743
BbeI G_GCGC'C
Cuts at: 1207 1455 1207
Size: 248 3525
BbsI GAAGACnn'nnnn_
Cuts at: 304 304
Size: 3773
BciVI GTATCCnnnnn_n'
Cuts at: 1671 2432 3773 1671
Size: 761 1341 1671
Fragments arranged by size:
1671 1341 761
BclI T'GATC_A
Cuts at: 1293 1293
Size: 3773
BglII A'GATC_T
Cuts at: 1288 3325 1288
Size: 2037 1736
BlpI GC'TnA_GC
Cuts at: 685 885 685
Size: 200 3573
Bme1580I G_kGCm'C
Cuts at: 1398 1491 2547 1398
Size: 93 1056 2624
Fragments arranged by size:
2624 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 985 1066 1401 3545 985
Size: 81 335 2144 1213
Fragments arranged by size:
2144 1213 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 3773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1828 2320 2618 949
Size: 879 492 298 2104
Fragments arranged by size:
2104 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 198 462 3477 198
Size: 264 3015 494
Fragments arranged by size:
3015 494 264
BsaAI yAC'GTr
Cuts at: 1126 1126
Size: 3773
BsaBI GATnn'nnATC
Cuts at: 6 1287 3324 6
Size: 1281 2037 455
Fragments arranged by size:
2037 1281 455
BsaHI Gr'CG_yC
Cuts at: 1204 1452 2154 3650 1204
Size: 248 702 1496 1327
Fragments arranged by size:
1496 1327 702 248
BsaWI w'CCGG_w
Cuts at: 962 1483 2161 2435 2582 2993 962
Size: 521 678 274 147 411 1742
Fragments arranged by size:
1742 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 337 337
Size: 3773
BseYI C'CCAG_C
Cuts at: 1198 1956 2533 3029 1198
Size: 758 577 496 1942
Fragments arranged by size:
1942 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 176
Size: 3773
BsiEI CG_ry'CG
Cuts at: 104 1315 1361 2569 104
Size: 1211 46 1208 1308
Fragments arranged by size:
1308 1211 1208 46
BsiHKAI G_wGCw'C
Cuts at: 787 1565 1755 2547 787
Size: 778 190 792 2013
Fragments arranged by size:
2013 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 749 749
Size: 3773
Bsp1286I G_dGCh'C
Cuts at: 787 1398 1491 1565 1755 1817 2547 787
Size: 611 93 74 190 62 730 2013
Fragments arranged by size:
2013 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2993 2993
Size: 3773
BspHI T'CATG_A
Cuts at: 504 620 2949 504
Size: 116 2329 1328
Fragments arranged by size:
2329 1328 116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 823 1037 1339 1720 823
Size: 214 302 381 2876
Fragments arranged by size:
2876 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2065 2119 3603 3768 50
Size: 2015 54 1484 165 55
Fragments arranged by size:
2015 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1685 1685
Size: 3773
BsrFI r'CCGG_y
Cuts at: 1771 1952 2161 1771
Size: 181 209 3383
Fragments arranged by size:
3383 209 181
BssSI C'ACGA_G
Cuts at: 2044 2402 2044
Size: 358 3415
BstAPI GCAn_nnn'nTGC
Cuts at: 1150 1150
Size: 3773
BstBI TT'CG_AA
Cuts at: 2134 2134
Size: 3773
BstYI r'GATC_y
Cuts at: 1 798 1265 1288 1623 1869 2881 2967 2979
Size: 797 467 23 335 246 1012 86 12
Cuts at: 2979 3325 1
Size: 346 449
Fragments arranged by size:
1012 797 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 297 317 538 107
Size: 190 20 221 3342
Fragments arranged by size:
3342 221 190 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1723 1904 2067 1723
Size: 181 163 3429
Fragments arranged by size:
3429 181 163
BtsI GCAGTG_nn'
Cuts at: 1016 1016
Size: 3773
DraI TTT'AAA
Cuts at: 772 772
Size: 3773
DrdI GACnn_nn'nnGTC
Cuts at: 308 1479 2337 308
Size: 1171 858 1744
Fragments arranged by size:
1744 1171 858
EaeI y'GGCC_r
Cuts at: 1358 1532 1923 1950 3295 1358
Size: 174 391 27 1345 1836
Fragments arranged by size:
1836 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1358 1358
Size: 3773
EarI CTCTTCn'nnn_
Cuts at: 1796 2006 1796
Size: 210 3563
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1981 2291 2437 3583 1981
Size: 310 146 1146 2171
Fragments arranged by size:
2171 1146 310 146
EcoICRI GAG'CTC
Cuts at: 785 785
Size: 3773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1598 2030 2777 3045 502
Size: 1096 432 747 268 1230
Fragments arranged by size:
1230 1096 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 912 912
Size: 3773
EcoRI G'AATT_C
Cuts at: 160 616 639 777 160
Size: 456 23 138 3156
Fragments arranged by size:
3156 456 138 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1792 1792
Size: 3773
FspI TGC'GCA
Cuts at: 1554 1554
Size: 3773
HaeII r_GCGC'y
Cuts at: 1207 1455 2477 3456 1207
Size: 248 1022 979 1524
Fragments arranged by size:
1524 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 264 296 1615 1645 1647 1677 264
Size: 32 1319 30 2 30 2360
Fragments arranged by size:
2360 1319 32 30 30 2
HincII GTy'rAC
Cuts at: 666 812 666
Size: 146 3627
HindIII A'AGCT_T
Cuts at: 453 453
Size: 3773
KasI G'GCGC_C
Cuts at: 1203 1451 1203
Size: 248 3525
KpnI G_GTAC'C
Cuts at: 793 793
Size: 3773
MluI A'CGCG_T
Cuts at: 3206 3206
Size: 3773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 168 2444 2628 168
Size: 2276 184 1313
Fragments arranged by size:
2276 1313 184
MscI TGG'CCA
Cuts at: 1534 1534
Size: 3773
MslI CAynn'nnrTG
Cuts at: 1762 1889 1762
Size: 127 3646
MspA1I CmG'CkG
Cuts at: 884 1198 1558 2571 2816 884
Size: 314 360 1013 245 1841
Fragments arranged by size:
1841 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1954 1954
Size: 3773
NarI GG'CG_CC
Cuts at: 1204 1452 1204
Size: 248 3525
NcoI C'CATG_G
Cuts at: 107 297 317 538 107
Size: 190 20 221 3342
Fragments arranged by size:
3342 221 190 20
NdeI CA'TA_TG
Cuts at: 490 490
Size: 3773
NgoMIV G'CCGG_C
Cuts at: 1952 1952
Size: 3773
NspI r_CATG'y
Cuts at: 237 311 826 1326 2233 237
Size: 74 515 500 907 1777
Fragments arranged by size:
1777 907 515 500 74
PciI A'CATG_T
Cuts at: 233 307 2229 233
Size: 74 1922 1777
Fragments arranged by size:
1922 1777 74
PflMI CCAn_nnn'nTGG
Cuts at: 574 706 574
Size: 132 3641
PmeI GTTT'AAAC
Cuts at: 772 772
Size: 3773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 3741
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 554 586 554
Size: 32 3741
PstI C_TGCA'G
Cuts at: 372 820 1505 372
Size: 448 685 2640
Fragments arranged by size:
2640 685 448
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3773
PvuII CAG'CTG
Cuts at: 1198 1558 1198
Size: 360 3413
RsrII CG'GwC_CG
Cuts at: 1968 1968
Size: 3773
SacI G_AGCT'C
Cuts at: 787 787
Size: 3773
SalI G'TCGA_C
Cuts at: 810 810
Size: 3773
SapI GCTCTTCn'nnn_
Cuts at: 1796 2006 1796
Size: 210 3563
SbfI CC_TGCA'GG
Cuts at: 820 820
Size: 3773
ScaI AGT'ACT
Cuts at: 3290 3290
Size: 3773
SfcI C'TryA_G
Cuts at: 33 368 417 529 816 1501 2494 2685 33
Size: 335 49 112 287 685 993 191 1121
Fragments arranged by size:
1121 993 685 335 287 191 112 49
SfoI GGC'GCC
Cuts at: 1205 1453 1205
Size: 248 3525
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
SmaI CCC'GGG
Cuts at: 795 795
Size: 3773
SmlI C'TyrA_G
Cuts at: 928 1843 2335 2597 928
Size: 915 492 262 2104
Fragments arranged by size:
2104 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3773
SphI G_CATG'C
Cuts at: 826 1326 826
Size: 500 3273
StyI C'CwwG_G
Cuts at: 107 297 317 341 538 907 1261 107
Size: 190 20 24 197 369 354 2619
Fragments arranged by size:
2619 369 354 197 190 24 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1356 1489 1948 2157 1356
Size: 133 459 209 2972
Fragments arranged by size:
2972 459 209 133
TatI w'GTAC_w
Cuts at: 435 591 3288 435
Size: 156 2697 920
Fragments arranged by size:
2697 920 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 3741
Tth111I GACn'n_nGTC
Cuts at: 1570 1570
Size: 3773
XbaI T'CTAG_A
Cuts at: 804 804
Size: 3773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1268 1268
Size: 3773
XmaI C'CCGG_G
Cuts at: 793 793
Size: 3773
XmnI GAAnn'nnTTC
Cuts at: 500 616 500
Size: 116 3657
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PmeI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI