Restriction map for
pF1KE0180
(Circular) (Six-base) MAPSORT of: pF1KE0180.seq Check: 9091 from: 1 to: 3767
pF1KE0180 3767 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 805 805
Size: 3767
Acc65I G'GTAC_C
Cuts at: 783 783
Size: 3767
AclI AA'CG_TT
Cuts at: 3607 3607
Size: 3767
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 606 1592 2024 2771 3039 606
Size: 986 432 747 268 1334
Fragments arranged by size:
1334 986 747 432 268
AflIII A'CryG_T
Cuts at: 1119 2223 3200 1119
Size: 1104 977 1686
Fragments arranged by size:
1686 1104 977
AgeI A'CCGG_T
Cuts at: 2155 2155
Size: 3767
AleI CACnn'nnGTG
Cuts at: 658 658
Size: 3767
AlwNI CAG_nnn'CTG
Cuts at: 252 1072 2639 252
Size: 820 1567 1380
Fragments arranged by size:
1567 1380 820
ApaLI G'TGCA_C
Cuts at: 2537 2537
Size: 3767
ApoI r'AATT_y
Cuts at: 771 3120 3376 771
Size: 2349 256 1162
Fragments arranged by size:
2349 1162 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3767
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
AvaI C'yCGr_G
Cuts at: 742 787 742
Size: 45 3722
BamHI G'GATC_C
Cuts at: 792 792
Size: 3767
BanI G'GyrC_C
Cuts at: 616 783 1197 1445 1480 616
Size: 167 414 248 35 2903
Fragments arranged by size:
2903 414 248 167 35
BanII G_rGCy'C
Cuts at: 781 1811 781
Size: 1030 2737
BbeI G_GCGC'C
Cuts at: 1201 1449 1201
Size: 248 3519
BbsI GAAGACnn'nnnn_
Cuts at: 522 522
Size: 3767
BciVI GTATCCnnnnn_n'
Cuts at: 1665 2426 3767 1665
Size: 761 1341 1665
Fragments arranged by size:
1665 1341 761
BclI T'GATC_A
Cuts at: 1287 1287
Size: 3767
BglII A'GATC_T
Cuts at: 1282 3319 1282
Size: 2037 1730
BlpI GC'TnA_GC
Cuts at: 163 879 163
Size: 716 3051
Bme1580I G_kGCm'C
Cuts at: 741 1392 1485 2541 741
Size: 651 93 1056 1967
Fragments arranged by size:
1967 1056 651 93
BmrI ACTGGGnnnn_n'
Cuts at: 979 1060 1395 3539 979
Size: 81 335 2144 1207
Fragments arranged by size:
2144 1207 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 3767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 274
Size: 3767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1822 2314 2612 943
Size: 879 492 298 2098
Fragments arranged by size:
2098 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 365 3471 365
Size: 3106 661
BsaAI yAC'GTr
Cuts at: 1120 1120
Size: 3767
BsaBI GATnn'nnATC
Cuts at: 6 1281 3318 6
Size: 1275 2037 455
Fragments arranged by size:
2037 1275 455
BsaHI Gr'CG_yC
Cuts at: 1198 1446 2148 3644 1198
Size: 248 702 1496 1321
Fragments arranged by size:
1496 1321 702 248
BsaWI w'CCGG_w
Cuts at: 956 1477 2155 2429 2576 2987 956
Size: 521 678 274 147 411 1736
Fragments arranged by size:
1736 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 114 144 368 398 114
Size: 30 224 30 3483
Fragments arranged by size:
3483 224 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 636 636
Size: 3767
BseYI C'CCAG_C
Cuts at: 1192 1950 2527 3023 1192
Size: 758 577 496 1936
Fragments arranged by size:
1936 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 288 349 403 650 173
Size: 115 61 54 247 3290
Fragments arranged by size:
3290 247 115 61 54
BsiEI CG_ry'CG
Cuts at: 104 1309 1355 2563 104
Size: 1205 46 1208 1308
Fragments arranged by size:
1308 1208 1205 46
BsiHKAI G_wGCw'C
Cuts at: 429 781 1559 1749 2541 429
Size: 352 778 190 792 1655
Fragments arranged by size:
1655 792 778 352 190
Bsp1286I G_dGCh'C
Cuts at: 429 741 781 1392 1485 1559 1749 1811 2541
Size: 312 40 611 93 74 190 62 730
Cuts at: 2541 429
Size: 1655
Fragments arranged by size:
1655 730 611 312 190 93 74 62
40
BspEI T'CCGG_A
Cuts at: 2987 2987
Size: 3767
BspHI T'CATG_A
Cuts at: 2943 2943
Size: 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 192 817 1031 1333 1714 192
Size: 625 214 302 381 2245
Fragments arranged by size:
2245 625 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2059 2113 3597 3762 50
Size: 2009 54 1484 165 55
Fragments arranged by size:
2009 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1679 1679
Size: 3767
BsrFI r'CCGG_y
Cuts at: 1765 1946 2155 1765
Size: 181 209 3377
Fragments arranged by size:
3377 209 181
BssSI C'ACGA_G
Cuts at: 2038 2396 2038
Size: 358 3409
BstAPI GCAn_nnn'nTGC
Cuts at: 1144 1144
Size: 3767
BstBI TT'CG_AA
Cuts at: 2128 2128
Size: 3767
BstYI r'GATC_y
Cuts at: 1 792 1259 1282 1617 1863 2875 2961 2973
Size: 791 467 23 335 246 1012 86 12
Cuts at: 2973 3319 1
Size: 346 449
Fragments arranged by size:
1012 791 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 284 284
Size: 3767
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 483 735 1717 1898 2061 483
Size: 252 982 181 163 2189
Fragments arranged by size:
2189 982 252 181 163
BtsI GCAGTG_nn'
Cuts at: 446 667 1010 446
Size: 221 343 3203
Fragments arranged by size:
3203 343 221
ClaI AT'CG_AT
Cuts at: 596 596
Size: 3767
DraI TTT'AAA
Cuts at: 766 766
Size: 3767
DrdI GACnn_nn'nnGTC
Cuts at: 1473 2331 1473
Size: 858 2909
EaeI y'GGCC_r
Cuts at: 684 1352 1526 1917 1944 3289 684
Size: 668 174 391 27 1345 1162
Fragments arranged by size:
1345 1162 668 391 174 27
EagI C'GGCC_G
Cuts at: 1352 1352
Size: 3767
EarI CTCTTCn'nnn_
Cuts at: 1790 2000 1790
Size: 210 3557
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1975 2285 2431 3577 1975
Size: 310 146 1146 2165
Fragments arranged by size:
2165 1146 310 146
EcoICRI GAG'CTC
Cuts at: 779 779
Size: 3767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 606 1592 2024 2771 3039 274
Size: 332 986 432 747 268 1002
Fragments arranged by size:
1002 986 747 432 332 268
EcoO109I rG'GnC_Cy
Cuts at: 174 906 174
Size: 732 3035
EcoRI G'AATT_C
Cuts at: 771 771
Size: 3767
EcoRV GAT'ATC
Cuts at: 600 600
Size: 3767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1786 1786
Size: 3767
FspI TGC'GCA
Cuts at: 1548 1548
Size: 3767
HaeII r_GCGC'y
Cuts at: 1201 1449 2471 3450 1201
Size: 248 1022 979 1518
Fragments arranged by size:
1518 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 114 146 1609 1639 1641 1671 114
Size: 32 1463 30 2 30 2210
Fragments arranged by size:
2210 1463 32 30 30 2
HincII GTy'rAC
Cuts at: 279 806 279
Size: 527 3240
HpaI GTT'AAC
Cuts at: 279 279
Size: 3767
KasI G'GCGC_C
Cuts at: 1197 1445 1197
Size: 248 3519
KpnI G_GTAC'C
Cuts at: 787 787
Size: 3767
MluI A'CGCG_T
Cuts at: 3200 3200
Size: 3767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 354 2438 2622 354
Size: 2084 184 1499
Fragments arranged by size:
2084 1499 184
MscI TGG'CCA
Cuts at: 1528 1528
Size: 3767
MslI CAynn'nnrTG
Cuts at: 658 754 1756 1883 658
Size: 96 1002 127 2542
Fragments arranged by size:
2542 1002 127 96
MspA1I CmG'CkG
Cuts at: 456 878 1192 1552 2565 2810 456
Size: 422 314 360 1013 245 1413
Fragments arranged by size:
1413 1013 422 360 314 245
NaeI GCC'GGC
Cuts at: 1948 1948
Size: 3767
NarI GG'CG_CC
Cuts at: 1198 1446 1198
Size: 248 3519
NgoMIV G'CCGG_C
Cuts at: 1946 1946
Size: 3767
NspI r_CATG'y
Cuts at: 820 1320 2227 820
Size: 500 907 2360
Fragments arranged by size:
2360 907 500
PciI A'CATG_T
Cuts at: 2223 2223
Size: 3767
PfoI T'CCnGG_A
Cuts at: 251 703 251
Size: 452 3315
PmeI GTTT'AAAC
Cuts at: 766 766
Size: 3767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2906 2938 2906
Size: 32 3735
PpuMI rG'GwC_Cy
Cuts at: 174 174
Size: 3767
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 117 149 117
Size: 32 3735
PstI C_TGCA'G
Cuts at: 674 814 1499 674
Size: 140 685 2942
Fragments arranged by size:
2942 685 140
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3767
PvuII CAG'CTG
Cuts at: 1192 1552 1192
Size: 360 3407
RsrII CG'GwC_CG
Cuts at: 1962 1962
Size: 3767
SacI G_AGCT'C
Cuts at: 781 781
Size: 3767
SalI G'TCGA_C
Cuts at: 804 804
Size: 3767
SanDI GG'GwC_CC
Cuts at: 174 174
Size: 3767
SapI GCTCTTCn'nnn_
Cuts at: 1790 2000 1790
Size: 210 3557
SbfI CC_TGCA'GG
Cuts at: 814 814
Size: 3767
ScaI AGT'ACT
Cuts at: 3284 3284
Size: 3767
SfcI C'TryA_G
Cuts at: 33 670 810 1495 2488 2679 33
Size: 637 140 685 993 191 1121
Fragments arranged by size:
1121 993 685 637 191 140
SfoI GGC'GCC
Cuts at: 1199 1447 1199
Size: 248 3519
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
SmaI CCC'GGG
Cuts at: 789 789
Size: 3767
SmlI C'TyrA_G
Cuts at: 922 1837 2329 2591 922
Size: 915 492 262 2098
Fragments arranged by size:
2098 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3767
SphI G_CATG'C
Cuts at: 820 1320 820
Size: 500 3267
StyI C'CwwG_G
Cuts at: 438 901 1255 438
Size: 463 354 2950
Fragments arranged by size:
2950 463 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 573 739 1350 1483 1942 2151 573
Size: 166 611 133 459 209 2189
Fragments arranged by size:
2189 611 459 209 166 133
TatI w'GTAC_w
Cuts at: 3282 3282
Size: 3767
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 368 400 3284 3316 368
Size: 32 2884 32 819
Fragments arranged by size:
2884 819 32 32
Tth111I GACn'n_nGTC
Cuts at: 201 1564 201
Size: 1363 2404
XbaI T'CTAG_A
Cuts at: 798 798
Size: 3767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 1262 453
Size: 809 2958
XmaI C'CCGG_G
Cuts at: 787 787
Size: 3767
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I CspCI DraIII EcoNI FseI FspAI
HindIII MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI