Restriction map for
pF1KE0178
(Circular) (Six-base) MAPSORT of: pF1KE0178.seq Check: 6769 from: 1 to: 4154
pF1KE0178 4154 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 869 869
Size: 4154
AccI GT'mk_AC
Cuts at: 1064 1192 1064
Size: 128 4026
Acc65I G'GTAC_C
Cuts at: 1170 1170
Size: 4154
AclI AA'CG_TT
Cuts at: 3994 3994
Size: 4154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 758 1979 2411 3158 3426 619
Size: 139 1221 432 747 268 1347
Fragments arranged by size:
1347 1221 747 432 268 139
AflIII A'CryG_T
Cuts at: 452 1506 2610 3587 452
Size: 1054 1104 977 1019
Fragments arranged by size:
1104 1054 1019 977
AgeI A'CCGG_T
Cuts at: 2542 2542
Size: 4154
AlwNI CAG_nnn'CTG
Cuts at: 157 234 916 1459 3026 157
Size: 77 682 543 1567 1285
Fragments arranged by size:
1567 1285 682 543 77
ApaLI G'TGCA_C
Cuts at: 2924 2924
Size: 4154
ApoI r'AATT_y
Cuts at: 217 631 1158 3507 3763 217
Size: 414 527 2349 256 608
Fragments arranged by size:
2349 608 527 414 256
AseI AT'TA_AT
Cuts at: 20 316 20
Size: 296 3858
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
AvaI C'yCGr_G
Cuts at: 1174 1174
Size: 4154
BamHI G'GATC_C
Cuts at: 1179 1179
Size: 4154
BanI G'GyrC_C
Cuts at: 1170 1584 1832 1867 1170
Size: 414 248 35 3457
Fragments arranged by size:
3457 414 248 35
BanII G_rGCy'C
Cuts at: 390 560 1168 2198 390
Size: 170 608 1030 2346
Fragments arranged by size:
2346 1030 608 170
BbeI G_GCGC'C
Cuts at: 1588 1836 1588
Size: 248 3906
BbsI GAAGACnn'nnnn_
Cuts at: 500 623 1017 500
Size: 123 394 3637
Fragments arranged by size:
3637 394 123
BciVI GTATCCnnnnn_n'
Cuts at: 281 2052 2813 4154 281
Size: 1771 761 1341 281
Fragments arranged by size:
1771 1341 761 281
BclI T'GATC_A
Cuts at: 656 1674 656
Size: 1018 3136
BfrBI ATG'CAT
Cuts at: 936 936
Size: 4154
BglII A'GATC_T
Cuts at: 1669 3706 1669
Size: 2037 2117
BlpI GC'TnA_GC
Cuts at: 1266 1266
Size: 4154
Bme1580I G_kGCm'C
Cuts at: 588 1779 1872 2928 588
Size: 1191 93 1056 1814
Fragments arranged by size:
1814 1191 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1366 1447 1782 3926 1366
Size: 81 335 2144 1594
Fragments arranged by size:
2144 1594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 827 2521 827
Size: 1694 2460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 404
Size: 4154
Bpu10I CC'TnA_GC
Cuts at: 368 773 368
Size: 405 3749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1330 2209 2701 2999 1330
Size: 879 492 298 2485
Fragments arranged by size:
2485 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 249 801 3858 249
Size: 552 3057 545
Fragments arranged by size:
3057 552 545
BsaAI yAC'GTr
Cuts at: 1507 1507
Size: 4154
BsaBI GATnn'nnATC
Cuts at: 6 1668 3705 6
Size: 1662 2037 455
Fragments arranged by size:
2037 1662 455
BsaHI Gr'CG_yC
Cuts at: 620 1585 1833 2535 4031 620
Size: 965 248 702 1496 743
Fragments arranged by size:
1496 965 743 702 248
BsaWI w'CCGG_w
Cuts at: 1343 1864 2542 2816 2963 3374 1343
Size: 521 678 274 147 411 2123
Fragments arranged by size:
2123 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 880 910 880
Size: 30 4124
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 819 819
Size: 4154
BseYI C'CCAG_C
Cuts at: 1579 2337 2914 3410 1579
Size: 758 577 496 2323
Fragments arranged by size:
2323 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 558 558
Size: 4154
BsiEI CG_ry'CG
Cuts at: 104 1696 1742 2950 104
Size: 1592 46 1208 1308
Fragments arranged by size:
1592 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 390 898 1168 1946 2136 2928 390
Size: 508 270 778 190 792 1616
Fragments arranged by size:
1616 792 778 508 270 190
Bsp1286I G_dGCh'C
Cuts at: 390 560 588 898 1168 1779 1872 1946 2136
Size: 170 28 310 270 611 93 74 190
Cuts at: 2136 2198 2928 390
Size: 62 730 1616
Fragments arranged by size:
1616 730 611 310 270 190 170 93
74 62 28
BspEI T'CCGG_A
Cuts at: 3374 3374
Size: 4154
BspHI T'CATG_A
Cuts at: 3330 3330
Size: 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 869 1204 1418 1720 2101 869
Size: 335 214 302 381 2922
Fragments arranged by size:
2922 381 335 302 214
BsrBI CCG'CTC
Cuts at: 50 777 2446 2500 3984 4149 50
Size: 727 1669 54 1484 165 55
Fragments arranged by size:
1669 1484 727 165 55 54
BsrDI GCAATG_nn'
Cuts at: 437 2066 437
Size: 1629 2525
BsrFI r'CCGG_y
Cuts at: 178 2152 2333 2542 178
Size: 1974 181 209 1790
Fragments arranged by size:
1974 1790 209 181
BssSI C'ACGA_G
Cuts at: 2425 2783 2425
Size: 358 3796
BstAPI GCAn_nnn'nTGC
Cuts at: 942 1531 942
Size: 589 3565
BstBI TT'CG_AA
Cuts at: 2515 2515
Size: 4154
BstXI CCAn_nnnn'nTGG
Cuts at: 190 190
Size: 4154
BstYI r'GATC_y
Cuts at: 1 111 1179 1646 1669 2004 2250 3262 3348
Size: 110 1068 467 23 335 246 1012 86
Cuts at: 3348 3360 3706 1
Size: 12 346 449
Fragments arranged by size:
1068 1012 467 449 346 335 246 110
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 716 716
Size: 4154
BtgI C'CryG_G
Cuts at: 107 799 107
Size: 692 3462
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1139 2104 2285 2448 1139
Size: 965 181 163 2845
Fragments arranged by size:
2845 965 181 163
BtsI GCAGTG_nn'
Cuts at: 326 1397 326
Size: 1071 3083
DraI TTT'AAA
Cuts at: 887 1153 887
Size: 266 3888
DrdI GACnn_nn'nnGTC
Cuts at: 1860 2718 1860
Size: 858 3296
EaeI y'GGCC_r
Cuts at: 180 1028 1739 1913 2304 2331 3676 180
Size: 848 711 174 391 27 1345 658
Fragments arranged by size:
1345 848 711 658 391 174 27
EagI C'GGCC_G
Cuts at: 1739 1739
Size: 4154
EarI CTCTTCn'nnn_
Cuts at: 680 2177 2387 680
Size: 1497 210 2447
Fragments arranged by size:
2447 1497 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2362 2672 2818 3964 2362
Size: 310 146 1146 2552
Fragments arranged by size:
2552 1146 310 146
EcoICRI GAG'CTC
Cuts at: 388 1166 388
Size: 778 3376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 619 758 1979 2411 3158 3426 404
Size: 215 139 1221 432 747 268 1132
Fragments arranged by size:
1221 1132 747 432 268 215 139
EcoNI CCTnn'n_nnAGG
Cuts at: 679 679
Size: 4154
EcoO109I rG'GnC_Cy
Cuts at: 1293 1293
Size: 4154
EcoRI G'AATT_C
Cuts at: 1158 1158
Size: 4154
EcoRV GAT'ATC
Cuts at: 1053 1053
Size: 4154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 915 2173 915
Size: 1258 2896
FspI TGC'GCA
Cuts at: 1935 1935
Size: 4154
HaeII r_GCGC'y
Cuts at: 402 765 1588 1836 2858 3837 402
Size: 363 823 248 1022 979 719
Fragments arranged by size:
1022 979 823 719 363 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 168 200 630 662 1996 2026 2028 2058 168
Size: 32 430 32 1334 30 2 30 2264
Fragments arranged by size:
2264 1334 430 32 32 30 30 2
HincII GTy'rAC
Cuts at: 123 1065 1193 123
Size: 942 128 3084
Fragments arranged by size:
3084 942 128
KasI G'GCGC_C
Cuts at: 1584 1832 1584
Size: 248 3906
KpnI G_GTAC'C
Cuts at: 1174 1174
Size: 4154
MfeI C'AATT_G
Cuts at: 209 209
Size: 4154
MluI A'CGCG_T
Cuts at: 3587 3587
Size: 4154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 659 2825 3009 659
Size: 2166 184 1804
Fragments arranged by size:
2166 1804 184
MscI TGG'CCA
Cuts at: 1030 1915 1030
Size: 885 3269
MslI CAynn'nnrTG
Cuts at: 188 485 2143 2270 188
Size: 297 1658 127 2072
Fragments arranged by size:
2072 1658 297 127
MspA1I CmG'CkG
Cuts at: 231 968 1265 1579 1939 2952 3197 231
Size: 737 297 314 360 1013 245 1188
Fragments arranged by size:
1188 1013 737 360 314 297 245
NaeI GCC'GGC
Cuts at: 2335 2335
Size: 4154
NarI GG'CG_CC
Cuts at: 1585 1833 1585
Size: 248 3906
NcoI C'CATG_G
Cuts at: 107 799 107
Size: 692 3462
NgoMIV G'CCGG_C
Cuts at: 2333 2333
Size: 4154
NsiI A_TGCA'T
Cuts at: 938 938
Size: 4154
NspI r_CATG'y
Cuts at: 198 456 1207 1707 2614 198
Size: 258 751 500 907 1738
Fragments arranged by size:
1738 907 751 500 258
PciI A'CATG_T
Cuts at: 452 2610 452
Size: 2158 1996
PflMI CCAn_nnn'nTGG
Cuts at: 651 758 1076 1113 651
Size: 107 318 37 3692
Fragments arranged by size:
3692 318 107 37
PfoI T'CCnGG_A
Cuts at: 611 611
Size: 4154
PmeI GTTT'AAAC
Cuts at: 1153 1153
Size: 4154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 766 798 3293 3325 766
Size: 32 2495 32 1595
Fragments arranged by size:
2495 1595 32 32
PstI C_TGCA'G
Cuts at: 1201 1886 1201
Size: 685 3469
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4154
PvuII CAG'CTG
Cuts at: 231 1579 1939 231
Size: 1348 360 2446
Fragments arranged by size:
2446 1348 360
RsrII CG'GwC_CG
Cuts at: 2349 2349
Size: 4154
SacI G_AGCT'C
Cuts at: 390 1168 390
Size: 778 3376
SalI G'TCGA_C
Cuts at: 1063 1191 1063
Size: 128 4026
SapI GCTCTTCn'nnn_
Cuts at: 2177 2387 2177
Size: 210 3944
SbfI CC_TGCA'GG
Cuts at: 1201 1201
Size: 4154
ScaI AGT'ACT
Cuts at: 3671 3671
Size: 4154
SfcI C'TryA_G
Cuts at: 33 1197 1882 2875 3066 33
Size: 1164 685 993 191 1121
Fragments arranged by size:
1164 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1586 1834 1586
Size: 248 3906
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
SmaI CCC'GGG
Cuts at: 1176 1176
Size: 4154
SmlI C'TyrA_G
Cuts at: 1309 2224 2716 2978 1309
Size: 915 492 262 2485
Fragments arranged by size:
2485 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4154
SphI G_CATG'C
Cuts at: 1207 1707 1207
Size: 500 3654
StyI C'CwwG_G
Cuts at: 107 308 625 799 1288 1642 107
Size: 201 317 174 489 354 2619
Fragments arranged by size:
2619 489 354 317 201 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 923 1087 1737 1870 2329 2538 923
Size: 164 650 133 459 209 2539
Fragments arranged by size:
2539 650 459 209 164 133
TatI w'GTAC_w
Cuts at: 449 3669 449
Size: 3220 934
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3671 3703 3671
Size: 32 4122
Tth111I GACn'n_nGTC
Cuts at: 1016 1951 1016
Size: 935 3219
XbaI T'CTAG_A
Cuts at: 1185 1185
Size: 4154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1649 1649
Size: 4154
XmaI C'CCGG_G
Cuts at: 1174 1174
Size: 4154
XmnI GAAnn'nnTTC
Cuts at: 689 689
Size: 4154
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII FseI FspAI HindIII HpaI NdeI NheI
NotI NruI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI