Restriction map for
pF1KE0176
(Circular) (Six-base) MAPSORT of: pF1KE0176.seq Check: 1227 from: 1 to: 5444
pF1KE0176 5444 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 1274 1800 2174 2413 2482 1274
Size: 526 374 239 69 4236
Fragments arranged by size:
4236 526 374 239 69
Acc65I G'GTAC_C
Cuts at: 2460 2460
Size: 5444
AclI AA'CG_TT
Cuts at: 5284 5284
Size: 5444
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 1174 1211 3269 3701 4448 4716 123
Size: 1051 37 2058 432 747 268 851
Fragments arranged by size:
2058 1051 851 747 432 268 37
AflII C'TTAA_G
Cuts at: 1252 1252
Size: 5444
AflIII A'CryG_T
Cuts at: 415 2049 2796 3900 4877 415
Size: 1634 747 1104 977 982
Fragments arranged by size:
1634 1104 982 977 747
AgeI A'CCGG_T
Cuts at: 3832 3832
Size: 5444
AleI CACnn'nnGTG
Cuts at: 957 957
Size: 5444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2209 2209
Size: 5444
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 389 421 816 848 389
Size: 32 395 32 4985
Fragments arranged by size:
4985 395 32 32
AlwNI CAG_nnn'CTG
Cuts at: 486 1407 2749 4316 486
Size: 921 1342 1567 1614
Fragments arranged by size:
1614 1567 1342 921
ApaLI G'TGCA_C
Cuts at: 4214 4214
Size: 5444
ApoI r'AATT_y
Cuts at: 367 490 689 1657 1705 1923 2383 2448 4797
Size: 123 199 968 48 218 460 65 2349
Cuts at: 4797 5053 367
Size: 256 758
Fragments arranged by size:
2349 968 758 460 256 218 199 123
65 48
AseI AT'TA_AT
Cuts at: 20 683 20
Size: 663 4781
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5444
AvaI C'yCGr_G
Cuts at: 2104 2292 2464 2104
Size: 188 172 5084
Fragments arranged by size:
5084 188 172
AvrII C'CTAG_G
Cuts at: 468 468
Size: 5444
BamHI G'GATC_C
Cuts at: 1371 2469 1371
Size: 1098 4346
BanI G'GyrC_C
Cuts at: 1609 2460 2874 3122 3157 1609
Size: 851 414 248 35 3896
Fragments arranged by size:
3896 851 414 248 35
BanII G_rGCy'C
Cuts at: 2458 3488 2458
Size: 1030 4414
BbeI G_GCGC'C
Cuts at: 2878 3126 2878
Size: 248 5196
BbsI GAAGACnn'nnnn_
Cuts at: 2441 2441
Size: 5444
BciVI GTATCCnnnnn_n'
Cuts at: 3342 4103 5444 3342
Size: 761 1341 3342
Fragments arranged by size:
3342 1341 761
BclI T'GATC_A
Cuts at: 2964 2964
Size: 5444
BfrBI ATG'CAT
Cuts at: 269 269
Size: 5444
BglI GCCn_nnn'nGGC
Cuts at: 2126 2126
Size: 5444
BglII A'GATC_T
Cuts at: 909 917 2959 4996 909
Size: 8 2042 2037 1357
Fragments arranged by size:
2042 2037 1357 8
BlpI GC'TnA_GC
Cuts at: 1958 2556 1958
Size: 598 4846
Bme1580I G_kGCm'C
Cuts at: 3069 3162 4218 3069
Size: 93 1056 4295
Fragments arranged by size:
4295 1056 93
BmgBI CAC'GTC
Cuts at: 1014 1014
Size: 5444
BmrI ACTGGGnnnn_n'
Cuts at: 2656 2737 3072 5216 2656
Size: 81 335 2144 2884
Fragments arranged by size:
2884 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2328 3811 2328
Size: 1483 3961
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 607 984 1717 2239 229
Size: 378 377 733 522 3434
Fragments arranged by size:
3434 733 522 378 377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 1353 2620 3499 3991 4289 103
Size: 1250 1267 879 492 298 1258
Fragments arranged by size:
1267 1258 1250 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1733 5148 1733
Size: 3415 2029
BsaAI yAC'GTr
Cuts at: 2050 2797 2050
Size: 747 4697
BsaBI GATnn'nnATC
Cuts at: 6 2958 4995 6
Size: 2952 2037 455
Fragments arranged by size:
2952 2037 455
BsaHI Gr'CG_yC
Cuts at: 2875 3123 3825 5321 2875
Size: 248 702 1496 2998
Fragments arranged by size:
2998 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2301 2633 3154 3832 4106 4253 4664 2301
Size: 332 521 678 274 147 411 3081
Fragments arranged by size:
3081 678 521 411 332 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 221 251 816 846 2137 2167 221
Size: 30 565 30 1291 30 3498
Fragments arranged by size:
3498 1291 565 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 722 725 728 731 734 737 1330 2304 2307
Size: 3 3 3 3 3 593 974 3
Cuts at: 2307 722
Size: 3859
Fragments arranged by size:
3859 974 593 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 608 2096 2238 2869 3627 4204 4700 608
Size: 1488 142 631 758 577 496 1352
Fragments arranged by size:
1488 1352 758 631 577 496 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2014 2370 2014
Size: 356 5088
BsiEI CG_ry'CG
Cuts at: 104 2986 3032 4240 104
Size: 2882 46 1208 1308
Fragments arranged by size:
2882 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 987 1792 2458 3236 3426 4218 987
Size: 805 666 778 190 792 2213
Fragments arranged by size:
2213 805 792 778 666 190
BsmI GAATG_Cn'
Cuts at: 1246 1257 1871 1246
Size: 11 614 4819
Fragments arranged by size:
4819 614 11
Bsp1286I G_dGCh'C
Cuts at: 987 1792 2458 3069 3162 3236 3426 3488 4218
Size: 805 666 611 93 74 190 62 730
Cuts at: 4218 987
Size: 2213
Fragments arranged by size:
2213 805 730 666 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4664 4664
Size: 5444
BspHI T'CATG_A
Cuts at: 4620 4620
Size: 5444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2036 2494 2708 3010 3391 2036
Size: 458 214 302 381 4089
Fragments arranged by size:
4089 458 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3736 3790 5274 5439 50
Size: 3686 54 1484 165 55
Fragments arranged by size:
3686 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 269 3356 269
Size: 3087 2357
BsrFI r'CCGG_y
Cuts at: 3442 3623 3832 3442
Size: 181 209 5054
Fragments arranged by size:
5054 209 181
BssSI C'ACGA_G
Cuts at: 3715 4073 3715
Size: 358 5086
BstAPI GCAn_nnn'nTGC
Cuts at: 2821 2821
Size: 5444
BstBI TT'CG_AA
Cuts at: 3805 3805
Size: 5444
BstYI r'GATC_y
Cuts at: 1 909 917 1371 1595 1950 2469 2936 2959
Size: 908 8 454 224 355 519 467 23
Cuts at: 2959 3294 3540 4552 4638 4650 4996 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 908 519 467 454 449 355 346
335 246 224 86 23 12 8
BstZ17I GTA'TAC
Cuts at: 1275 1801 1275
Size: 526 4918
Bsu36I CC'TnA_GG
Cuts at: 696 696
Size: 5444
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3394 3575 3738 3394
Size: 181 163 5100
Fragments arranged by size:
5100 181 163
BtsI GCAGTG_nn'
Cuts at: 987 1580 2214 2687 987
Size: 593 634 473 3744
Fragments arranged by size:
3744 634 593 473
DraI TTT'AAA
Cuts at: 1827 2443 1827
Size: 616 4828
DrdI GACnn_nn'nnGTC
Cuts at: 3150 4008 3150
Size: 858 4586
EaeI y'GGCC_r
Cuts at: 3029 3203 3594 3621 4966 3029
Size: 174 391 27 1345 3507
Fragments arranged by size:
3507 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3029 3029
Size: 5444
EarI CTCTTCn'nnn_
Cuts at: 1187 1320 3467 3677 1187
Size: 133 2147 210 2954
Fragments arranged by size:
2954 2147 210 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3652 3962 4108 5254 3652
Size: 310 146 1146 3842
Fragments arranged by size:
3842 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2456 2456
Size: 5444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 229 607 984 1174 1211 1717 2239 3269
Size: 106 378 377 190 37 506 522 1030
Cuts at: 3269 3701 4448 4716 123
Size: 432 747 268 851
Fragments arranged by size:
1030 851 747 522 506 432 378 377
268 190 106 37
EcoNI CCTnn'n_nnAGG
Cuts at: 1312 1380 1312
Size: 68 5376
EcoO109I rG'GnC_Cy
Cuts at: 143 472 562 2583 143
Size: 329 90 2021 3004
Fragments arranged by size:
3004 2021 329 90
EcoRI G'AATT_C
Cuts at: 689 2448 689
Size: 1759 3685
EcoRV GAT'ATC
Cuts at: 857 1438 857
Size: 581 4863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1927 3463 1927
Size: 1536 3908
FspI TGC'GCA
Cuts at: 3225 3225
Size: 5444
HaeII r_GCGC'y
Cuts at: 2878 3126 4148 5127 2878
Size: 248 1022 979 3195
Fragments arranged by size:
3195 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 221 253 1356 1388 1468 1500 3286 3316 3318
Size: 32 1103 32 80 32 1786 30 2
Cuts at: 3318 3348 221
Size: 30 2317
Fragments arranged by size:
2317 1786 1103 80 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2175 2483 2175
Size: 308 5136
KasI G'GCGC_C
Cuts at: 2874 3122 2874
Size: 248 5196
KpnI G_GTAC'C
Cuts at: 2464 2464
Size: 5444
MfeI C'AATT_G
Cuts at: 1681 1681
Size: 5444
MluI A'CGCG_T
Cuts at: 4877 4877
Size: 5444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 523 4115 4299 523
Size: 3592 184 1668
Fragments arranged by size:
3592 1668 184
MscI TGG'CCA
Cuts at: 3205 3205
Size: 5444
MslI CAynn'nnrTG
Cuts at: 883 957 3433 3560 883
Size: 74 2476 127 2767
Fragments arranged by size:
2767 2476 127 74
MspA1I CmG'CkG
Cuts at: 1524 2096 2270 2555 2869 3229 4242 4487 1524
Size: 572 174 285 314 360 1013 245 2481
Fragments arranged by size:
2481 1013 572 360 314 285 245 174
NaeI GCC'GGC
Cuts at: 3625 3625
Size: 5444
NarI GG'CG_CC
Cuts at: 2875 3123 2875
Size: 248 5196
NgoMIV G'CCGG_C
Cuts at: 3623 3623
Size: 5444
NsiI A_TGCA'T
Cuts at: 271 271
Size: 5444
NspI r_CATG'y
Cuts at: 419 1281 2497 2997 3904 419
Size: 862 1216 500 907 1959
Fragments arranged by size:
1959 1216 907 862 500
PciI A'CATG_T
Cuts at: 415 3900 415
Size: 3485 1959
PflMI CCAn_nnn'nTGG
Cuts at: 2405 2405
Size: 5444
PmeI GTTT'AAAC
Cuts at: 2443 2443
Size: 5444
PmlI CAC'GTG
Cuts at: 2050 2050
Size: 5444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 816 848 4583 4615 816
Size: 32 3735 32 1645
Fragments arranged by size:
3735 1645 32 32
PpuMI rG'GwC_Cy
Cuts at: 143 562 143
Size: 419 5025
PstI C_TGCA'G
Cuts at: 1022 1545 2491 3176 1022
Size: 523 946 685 3290
Fragments arranged by size:
3290 946 685 523
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5444
PvuII CAG'CTG
Cuts at: 1524 2869 3229 1524
Size: 1345 360 3739
Fragments arranged by size:
3739 1345 360
RsrII CG'GwC_CG
Cuts at: 3639 3639
Size: 5444
SacI G_AGCT'C
Cuts at: 2458 2458
Size: 5444
SalI G'TCGA_C
Cuts at: 2173 2481 2173
Size: 308 5136
SapI GCTCTTCn'nnn_
Cuts at: 3467 3677 3467
Size: 210 5234
SbfI CC_TGCA'GG
Cuts at: 2491 2491
Size: 5444
ScaI AGT'ACT
Cuts at: 4961 4961
Size: 5444
SfcI C'TryA_G
Cuts at: 33 1018 1301 1541 1845 2336 2487 3172 4165
Size: 985 283 240 304 491 151 685 993
Cuts at: 4165 4356 33
Size: 191 1121
Fragments arranged by size:
1121 993 985 685 491 304 283 240
191 151
SfoI GGC'GCC
Cuts at: 2876 3124 2876
Size: 248 5196
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5444
SmaI CCC'GGG
Cuts at: 2466 2466
Size: 5444
SmlI C'TyrA_G
Cuts at: 118 1252 1332 2599 3514 4006 4268 118
Size: 1134 80 1267 915 492 262 1294
Fragments arranged by size:
1294 1267 1134 915 492 262 80
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5444
SphI G_CATG'C
Cuts at: 2497 2997 2497
Size: 500 4944
SspI AAT'ATT
Cuts at: 1122 1122
Size: 5444
StuI AGG'CCT
Cuts at: 2224 2224
Size: 5444
StyI C'CwwG_G
Cuts at: 468 1915 2578 2932 468
Size: 1447 663 354 2980
Fragments arranged by size:
2980 1447 663 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1085 1675 2113 3027 3160 3619 3828 1085
Size: 590 438 914 133 459 209 2701
Fragments arranged by size:
2701 914 590 459 438 209 133
TatI w'GTAC_w
Cuts at: 2255 4959 2255
Size: 2704 2740
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 852 884 2137 2169 4961 4993 852
Size: 32 1253 32 2792 32 1303
Fragments arranged by size:
2792 1303 1253 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3241 3241
Size: 5444
XbaI T'CTAG_A
Cuts at: 1446 2475 1446
Size: 1029 4415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 934 959 2939 934
Size: 25 1980 3439
Fragments arranged by size:
3439 1980 25
XmaI C'CCGG_G
Cuts at: 2464 2464
Size: 5444
XmnI GAAnn'nnTTC
Cuts at: 1910 1910
Size: 5444
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PciI PflMI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI ApaI AscI BaeI BbvCI
BcgI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII
BstXI BtgI ClaI CspCI DraIII FseI FspAI HindIII
HpaI NcoI NdeI NheI NotI NruI PacI PasI
PfoI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI