Restriction map for
pF1KE0172
(Circular) (Six-base) MAPSORT of: pF1KE0172.seq Check: 4949 from: 1 to: 4064
pF1KE0172 4064 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AatII G_ACGT'C
Cuts at: 949 949
Size: 4064
AccI GT'mk_AC
Cuts at: 704 1102 704
Size: 398 3666
Acc65I G'GTAC_C
Cuts at: 1080 1080
Size: 4064
AclI AA'CG_TT
Cuts at: 3904 3904
Size: 4064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1889 2321 3068 3336 1889
Size: 432 747 268 2617
Fragments arranged by size:
2617 747 432 268
AflII C'TTAA_G
Cuts at: 543 543
Size: 4064
AflIII A'CryG_T
Cuts at: 1416 2520 3497 1416
Size: 1104 977 1983
Fragments arranged by size:
1983 1104 977
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 622 1369 2936 622
Size: 747 1567 1750
Fragments arranged by size:
1750 1567 747
ApaI G_GGCC'C
Cuts at: 232 232
Size: 4064
ApaLI G'TGCA_C
Cuts at: 2834 2834
Size: 4064
ApoI r'AATT_y
Cuts at: 241 1068 3417 3673 241
Size: 827 2349 256 632
Fragments arranged by size:
2349 827 632 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 1084 1084
Size: 4064
BamHI G'GATC_C
Cuts at: 1089 1089
Size: 4064
BanI G'GyrC_C
Cuts at: 918 1080 1494 1742 1777 918
Size: 162 414 248 35 3205
Fragments arranged by size:
3205 414 248 162 35
BanII G_rGCy'C
Cuts at: 232 1007 1078 2108 232
Size: 775 71 1030 2188
Fragments arranged by size:
2188 1030 775 71
BbeI G_GCGC'C
Cuts at: 1498 1746 1498
Size: 248 3816
BbsI GAAGACnn'nnnn_
Cuts at: 399 399
Size: 4064
BbvCI CC'TCA_GC
Cuts at: 524 524
Size: 4064
BciVI GTATCCnnnnn_n'
Cuts at: 1962 2723 4064 1962
Size: 761 1341 1962
Fragments arranged by size:
1962 1341 761
BclI T'GATC_A
Cuts at: 1584 1584
Size: 4064
BglII A'GATC_T
Cuts at: 1579 3616 1579
Size: 2037 2027
BlpI GC'TnA_GC
Cuts at: 1176 1176
Size: 4064
Bme1580I G_kGCm'C
Cuts at: 232 1689 1782 2838 232
Size: 1457 93 1056 1458
Fragments arranged by size:
1458 1457 1056 93
BmgBI CAC'GTC
Cuts at: 145 145
Size: 4064
BmrI ACTGGGnnnn_n'
Cuts at: 1276 1357 1692 3836 1276
Size: 81 335 2144 1504
Fragments arranged by size:
2144 1504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 609 2431 609
Size: 1822 2242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 604
Size: 4064
Bpu10I CC'TnA_GC
Cuts at: 524 524
Size: 4064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 2119 2611 2909 1240
Size: 879 492 298 2395
Fragments arranged by size:
2395 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3768 3768
Size: 4064
BsaAI yAC'GTr
Cuts at: 1417 1417
Size: 4064
BsaBI GATnn'nnATC
Cuts at: 6 667 1578 3615 6
Size: 661 911 2037 455
Fragments arranged by size:
2037 911 661 455
BsaHI Gr'CG_yC
Cuts at: 946 1495 1743 2445 3941 946
Size: 549 248 702 1496 1069
Fragments arranged by size:
1496 1069 702 549 248
BsaWI w'CCGG_w
Cuts at: 551 1253 1774 2452 2726 2873 3284 551
Size: 702 521 678 274 147 411 1331
Fragments arranged by size:
1331 702 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 465 511 465
Size: 46 4018
BseYI C'CCAG_C
Cuts at: 1025 1489 2247 2824 3320 1025
Size: 464 758 577 496 1769
Fragments arranged by size:
1769 758 577 496 464
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 925 99
Size: 826 3238
BsiEI CG_ry'CG
Cuts at: 104 1606 1652 2860 104
Size: 1502 46 1208 1308
Fragments arranged by size:
1502 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 337 1078 1856 2046 2838 337
Size: 741 778 190 792 1563
Fragments arranged by size:
1563 792 778 741 190
BsiWI C'GTAC_G
Cuts at: 233 233
Size: 4064
BsmI GAATG_Cn'
Cuts at: 934 934
Size: 4064
BsmBI CGTCTCn'nnnn_
Cuts at: 953 953
Size: 4064
Bsp1286I G_dGCh'C
Cuts at: 232 337 1007 1078 1689 1782 1856 2046 2108
Size: 105 670 71 611 93 74 190 62
Cuts at: 2108 2838 232
Size: 730 1458
Fragments arranged by size:
1458 730 670 611 190 105 93 74
71 62
BspEI T'CCGG_A
Cuts at: 551 3284 551
Size: 2733 1331
BspHI T'CATG_A
Cuts at: 3240 3240
Size: 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1114 1328 1630 2011 1114
Size: 214 302 381 3167
Fragments arranged by size:
3167 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2356 2410 3894 4059 50
Size: 2306 54 1484 165 55
Fragments arranged by size:
2306 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1976 1976
Size: 4064
BsrFI r'CCGG_y
Cuts at: 2062 2243 2452 2062
Size: 181 209 3674
Fragments arranged by size:
3674 209 181
BssSI C'ACGA_G
Cuts at: 493 2335 2693 493
Size: 1842 358 1864
Fragments arranged by size:
1864 1842 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1441 1441
Size: 4064
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstXI CCAn_nnnn'nTGG
Cuts at: 829 829
Size: 4064
BstYI r'GATC_y
Cuts at: 1 138 438 662 1089 1556 1579 1914 2160
Size: 137 300 224 427 467 23 335 246
Cuts at: 2160 3172 3258 3270 3616 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 427 346 335 300 246
224 137 86 23 12
BtgI C'CryG_G
Cuts at: 170 170
Size: 4064
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2014 2195 2358 2014
Size: 181 163 3720
Fragments arranged by size:
3720 181 163
BtsI GCAGTG_nn'
Cuts at: 392 1307 392
Size: 915 3149
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 131 166 131
Size: 35 4029
DraI TTT'AAA
Cuts at: 1063 1063
Size: 4064
DrdI GACnn_nn'nnGTC
Cuts at: 1770 2628 1770
Size: 858 3206
EaeI y'GGCC_r
Cuts at: 179 1649 1823 2214 2241 3586 179
Size: 1470 174 391 27 1345 657
Fragments arranged by size:
1470 1345 657 391 174 27
EagI C'GGCC_G
Cuts at: 1649 1649
Size: 4064
EarI CTCTTCn'nnn_
Cuts at: 211 1012 2087 2297 211
Size: 801 1075 210 1978
Fragments arranged by size:
1978 1075 801 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2272 2582 2728 3874 2272
Size: 310 146 1146 2462
Fragments arranged by size:
2462 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1076 1076
Size: 4064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1889 2321 3068 3336 604
Size: 1285 432 747 268 1332
Fragments arranged by size:
1332 1285 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 381 1203 381
Size: 822 3242
EcoRI G'AATT_C
Cuts at: 1068 1068
Size: 4064
EcoRV GAT'ATC
Cuts at: 471 471
Size: 4064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 4064
FspI TGC'GCA
Cuts at: 1845 1845
Size: 4064
HaeII r_GCGC'y
Cuts at: 1498 1746 2768 3747 1498
Size: 248 1022 979 1815
Fragments arranged by size:
1815 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 460 492 739 771 952 984 1906 1936 1938
Size: 32 247 32 181 32 922 30 2
Cuts at: 1938 1968 460
Size: 30 2556
Fragments arranged by size:
2556 922 247 181 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 971 1103 971
Size: 132 3932
KasI G'GCGC_C
Cuts at: 1494 1742 1494
Size: 248 3816
KpnI G_GTAC'C
Cuts at: 1084 1084
Size: 4064
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 437 811 2735 2919 437
Size: 374 1924 184 1582
Fragments arranged by size:
1924 1582 374 184
MscI TGG'CCA
Cuts at: 181 1825 181
Size: 1644 2420
MslI CAynn'nnrTG
Cuts at: 121 169 827 2053 2180 121
Size: 48 658 1226 127 2005
Fragments arranged by size:
2005 1226 658 127 48
MspA1I CmG'CkG
Cuts at: 1175 1489 1849 2862 3107 1175
Size: 314 360 1013 245 2132
Fragments arranged by size:
2132 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2245 2245
Size: 4064
NarI GG'CG_CC
Cuts at: 1495 1743 1495
Size: 248 3816
NgoMIV G'CCGG_C
Cuts at: 2243 2243
Size: 4064
NspI r_CATG'y
Cuts at: 1117 1617 2524 1117
Size: 500 907 2657
Fragments arranged by size:
2657 907 500
PciI A'CATG_T
Cuts at: 2520 2520
Size: 4064
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1033 1065 3203 3235 1033
Size: 32 2138 32 1862
Fragments arranged by size:
2138 1862 32 32
PspOMI G'GGCC_C
Cuts at: 228 228
Size: 4064
PstI C_TGCA'G
Cuts at: 446 1111 1796 446
Size: 665 685 2714
Fragments arranged by size:
2714 685 665
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 1489 1849 1489
Size: 360 3704
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 1078 1078
Size: 4064
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 1012 2087 2297 1012
Size: 1075 210 2779
Fragments arranged by size:
2779 1075 210
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SfcI C'TryA_G
Cuts at: 33 442 1107 1792 2785 2976 33
Size: 409 665 685 993 191 1121
Fragments arranged by size:
1121 993 685 665 409 191
SfoI GGC'GCC
Cuts at: 1496 1744 1496
Size: 248 3816
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 543 1219 2134 2626 2888 543
Size: 676 915 492 262 1719
Fragments arranged by size:
1719 915 676 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 1117 1617 1117
Size: 500 3564
StyI C'CwwG_G
Cuts at: 377 1198 1552 377
Size: 821 354 2889
Fragments arranged by size:
2889 821 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 150 1647 1780 2239 2448 150
Size: 1497 133 459 209 1766
Fragments arranged by size:
1766 1497 459 209 133
TatI w'GTAC_w
Cuts at: 3579 3579
Size: 4064
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 4032
Tth111I GACn'n_nGTC
Cuts at: 709 1861 709
Size: 1152 2912
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1559 1559
Size: 4064
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
XmnI GAAnn'nnTTC
Cuts at: 220 220
Size: 4064
ZraI GAC'GTC
Cuts at: 947 947
Size: 4064
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BglI BmtI BsaXI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI