Restriction map for
pF1KE0165
(Circular) (Six-base) MAPSORT of: pF1KE0165.seq Check: 5355 from: 1 to: 3674
pF1KE0165 3674 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 712 712
Size: 3674
Acc65I G'GTAC_C
Cuts at: 690 690
Size: 3674
AclI AA'CG_TT
Cuts at: 3514 3514
Size: 3674
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 418 424 1499 1931 2678 2946 181
Size: 237 6 1075 432 747 268 909
Fragments arranged by size:
1075 909 747 432 268 237 6
AflIII A'CryG_T
Cuts at: 1026 2130 3107 1026
Size: 1104 977 1593
Fragments arranged by size:
1593 1104 977
AgeI A'CCGG_T
Cuts at: 2062 2062
Size: 3674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 3674
AlwNI CAG_nnn'CTG
Cuts at: 300 979 2546 300
Size: 679 1567 1428
Fragments arranged by size:
1567 1428 679
ApaI G_GGCC'C
Cuts at: 486 486
Size: 3674
ApaLI G'TGCA_C
Cuts at: 2444 2444
Size: 3674
ApoI r'AATT_y
Cuts at: 678 3027 3283 678
Size: 2349 256 1069
Fragments arranged by size:
2349 1069 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3674
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3674
AvaI C'yCGr_G
Cuts at: 694 694
Size: 3674
BamHI G'GATC_C
Cuts at: 699 699
Size: 3674
BanI G'GyrC_C
Cuts at: 690 1104 1352 1387 690
Size: 414 248 35 2977
Fragments arranged by size:
2977 414 248 35
BanII G_rGCy'C
Cuts at: 486 496 688 1718 486
Size: 10 192 1030 2442
Fragments arranged by size:
2442 1030 192 10
BbeI G_GCGC'C
Cuts at: 1108 1356 1108
Size: 248 3426
BbsI GAAGACnn'nnnn_
Cuts at: 557 557
Size: 3674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3640
BciVI GTATCCnnnnn_n'
Cuts at: 1572 2333 3674 1572
Size: 761 1341 1572
Fragments arranged by size:
1572 1341 761
BclI T'GATC_A
Cuts at: 1194 1194
Size: 3674
BglII A'GATC_T
Cuts at: 1189 3226 1189
Size: 2037 1637
BlpI GC'TnA_GC
Cuts at: 786 786
Size: 3674
Bme1580I G_kGCm'C
Cuts at: 291 486 1299 1392 2448 291
Size: 195 813 93 1056 1517
Fragments arranged by size:
1517 1056 813 195 93
BmrI ACTGGGnnnn_n'
Cuts at: 886 967 1302 3446 886
Size: 81 335 2144 1114
Fragments arranged by size:
2144 1114 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 3674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 609
Size: 3674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 850 1729 2221 2519 517
Size: 333 879 492 298 1672
Fragments arranged by size:
1672 879 492 333 298
BsaI GGTCTCn'nnnn_
Cuts at: 3378 3378
Size: 3674
BsaAI yAC'GTr
Cuts at: 1027 1027
Size: 3674
BsaBI GATnn'nnATC
Cuts at: 6 1188 3225 6
Size: 1182 2037 455
Fragments arranged by size:
2037 1182 455
BsaHI Gr'CG_yC
Cuts at: 470 1105 1353 2055 3551 470
Size: 635 248 702 1496 593
Fragments arranged by size:
1496 702 635 593 248
BsaWI w'CCGG_w
Cuts at: 863 1384 2062 2336 2483 2894 863
Size: 521 678 274 147 411 1643
Fragments arranged by size:
1643 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 595 625 595
Size: 30 3644
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 355 226
Size: 129 3545
BseYI C'CCAG_C
Cuts at: 194 489 1099 1857 2434 2930 194
Size: 295 610 758 577 496 938
Fragments arranged by size:
938 758 610 577 496 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 298
Size: 3674
BsiEI CG_ry'CG
Cuts at: 104 1216 1262 2470 104
Size: 1112 46 1208 1308
Fragments arranged by size:
1308 1208 1112 46
BsiHKAI G_wGCw'C
Cuts at: 209 688 1466 1656 2448 209
Size: 479 778 190 792 1435
Fragments arranged by size:
1435 792 778 479 190
BsmI GAATG_Cn'
Cuts at: 196 196
Size: 3674
Bsp1286I G_dGCh'C
Cuts at: 209 291 486 496 688 1299 1392 1466 1656
Size: 82 195 10 192 611 93 74 190
Cuts at: 1656 1718 2448 209
Size: 62 730 1435
Fragments arranged by size:
1435 730 611 195 192 190 93 82
74 62 10
BspEI T'CCGG_A
Cuts at: 2894 2894
Size: 3674
BspHI T'CATG_A
Cuts at: 2850 2850
Size: 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 724 938 1240 1621 724
Size: 214 302 381 2777
Fragments arranged by size:
2777 381 302 214
BsrBI CCG'CTC
Cuts at: 50 567 1966 2020 3504 3669 50
Size: 517 1399 54 1484 165 55
Fragments arranged by size:
1484 1399 517 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1586 1586
Size: 3674
BsrFI r'CCGG_y
Cuts at: 1672 1853 2062 1672
Size: 181 209 3284
Fragments arranged by size:
3284 209 181
BssSI C'ACGA_G
Cuts at: 208 1945 2303 208
Size: 1737 358 1579
Fragments arranged by size:
1737 1579 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1051 1051
Size: 3674
BstBI TT'CG_AA
Cuts at: 2035 2035
Size: 3674
BstYI r'GATC_y
Cuts at: 1 516 699 1166 1189 1524 1770 2782 2868
Size: 515 183 467 23 335 246 1012 86
Cuts at: 2868 2880 3226 1
Size: 12 346 449
Fragments arranged by size:
1012 515 467 449 346 335 246 183
86 23 12
BtgI C'CryG_G
Cuts at: 270 420 512 270
Size: 150 92 3432
Fragments arranged by size:
3432 150 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1624 1805 1968 1624
Size: 181 163 3330
Fragments arranged by size:
3330 181 163
BtsI GCAGTG_nn'
Cuts at: 137 917 137
Size: 780 2894
DraI TTT'AAA
Cuts at: 673 673
Size: 3674
DraIII CAC_nnn'GTG
Cuts at: 507 507
Size: 3674
DrdI GACnn_nn'nnGTC
Cuts at: 1380 2238 1380
Size: 858 2816
EaeI y'GGCC_r
Cuts at: 176 1259 1433 1824 1851 3196 176
Size: 1083 174 391 27 1345 654
Fragments arranged by size:
1345 1083 654 391 174 27
EagI C'GGCC_G
Cuts at: 1259 1259
Size: 3674
EarI CTCTTCn'nnn_
Cuts at: 157 1697 1907 157
Size: 1540 210 1924
Fragments arranged by size:
1924 1540 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1882 2192 2338 3484 1882
Size: 310 146 1146 2072
Fragments arranged by size:
2072 1146 310 146
EcoICRI GAG'CTC
Cuts at: 686 686
Size: 3674
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 418 424 609 1499 1931 2678 2946 181
Size: 237 6 185 890 432 747 268 909
Fragments arranged by size:
909 890 747 432 268 237 185 6
EcoO109I rG'GnC_Cy
Cuts at: 297 393 483 813 297
Size: 96 90 330 3158
Fragments arranged by size:
3158 330 96 90
EcoRI G'AATT_C
Cuts at: 678 678
Size: 3674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1693 1693
Size: 3674
FspI TGC'GCA
Cuts at: 1455 1455
Size: 3674
HaeII r_GCGC'y
Cuts at: 438 1108 1356 2378 3357 438
Size: 670 248 1022 979 755
Fragments arranged by size:
1022 979 755 670 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 443 475 595 627 1516 1546 1548 1578 443
Size: 32 120 32 889 30 2 30 2539
Fragments arranged by size:
2539 889 120 32 32 30 30 2
HincII GTy'rAC
Cuts at: 713 713
Size: 3674
KasI G'GCGC_C
Cuts at: 1104 1352 1104
Size: 248 3426
KpnI G_GTAC'C
Cuts at: 694 694
Size: 3674
MluI A'CGCG_T
Cuts at: 3107 3107
Size: 3674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 682 2345 2529 682
Size: 1663 184 1827
Fragments arranged by size:
1827 1663 184
MscI TGG'CCA
Cuts at: 178 1435 178
Size: 1257 2417
MslI CAynn'nnrTG
Cuts at: 1663 1790 1663
Size: 127 3547
MspA1I CmG'CkG
Cuts at: 785 1099 1459 2472 2717 785
Size: 314 360 1013 245 1742
Fragments arranged by size:
1742 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1855 1855
Size: 3674
NarI GG'CG_CC
Cuts at: 1105 1353 1105
Size: 248 3426
NcoI C'CATG_G
Cuts at: 512 512
Size: 3674
NgoMIV G'CCGG_C
Cuts at: 1853 1853
Size: 3674
NspI r_CATG'y
Cuts at: 727 1227 2134 727
Size: 500 907 2267
Fragments arranged by size:
2267 907 500
PciI A'CATG_T
Cuts at: 2130 2130
Size: 3674
PmeI GTTT'AAAC
Cuts at: 673 673
Size: 3674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2813 2845 2813
Size: 32 3642
PpuMI rG'GwC_Cy
Cuts at: 297 393 297
Size: 96 3578
PspOMI G'GGCC_C
Cuts at: 482 482
Size: 3674
PstI C_TGCA'G
Cuts at: 266 296 721 1406 266
Size: 30 425 685 2534
Fragments arranged by size:
2534 685 425 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3674
PvuII CAG'CTG
Cuts at: 1099 1459 1099
Size: 360 3314
RsrII CG'GwC_CG
Cuts at: 1869 1869
Size: 3674
SacI G_AGCT'C
Cuts at: 688 688
Size: 3674
SalI G'TCGA_C
Cuts at: 711 711
Size: 3674
SapI GCTCTTCn'nnn_
Cuts at: 157 1697 1907 157
Size: 1540 210 1924
Fragments arranged by size:
1924 1540 210
SbfI CC_TGCA'GG
Cuts at: 296 721 296
Size: 425 3249
ScaI AGT'ACT
Cuts at: 3191 3191
Size: 3674
SfcI C'TryA_G
Cuts at: 33 262 292 717 1402 2395 2586 33
Size: 229 30 425 685 993 191 1121
Fragments arranged by size:
1121 993 685 425 229 191 30
SfoI GGC'GCC
Cuts at: 1106 1354 1106
Size: 248 3426
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3674
SmaI CCC'GGG
Cuts at: 696 696
Size: 3674
SmlI C'TyrA_G
Cuts at: 532 829 1744 2236 2498 532
Size: 297 915 492 262 1708
Fragments arranged by size:
1708 915 492 297 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3674
SphI G_CATG'C
Cuts at: 727 1227 727
Size: 500 3174
StyI C'CwwG_G
Cuts at: 179 185 320 512 808 1162 179
Size: 6 135 192 296 354 2691
Fragments arranged by size:
2691 354 296 192 135 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 571 1257 1390 1849 2058 571
Size: 686 133 459 209 2187
Fragments arranged by size:
2187 686 459 209 133
TatI w'GTAC_w
Cuts at: 3189 3189
Size: 3674
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 493 525 3191 3223 493
Size: 32 2666 32 944
Fragments arranged by size:
2666 944 32 32
Tth111I GACn'n_nGTC
Cuts at: 1471 1471
Size: 3674
XbaI T'CTAG_A
Cuts at: 705 705
Size: 3674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 327 615 1169 327
Size: 288 554 2832
Fragments arranged by size:
2832 554 288
XmaI C'CCGG_G
Cuts at: 694 694
Size: 3674
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI