Restriction map for
pF1KE0164
(Circular) (Six-base) MAPSORT of: pF1KE0164.seq Check: 9755 from: 1 to: 3527
pF1KE0164 3527 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 243 243
Size: 3527
AccI GT'mk_AC
Cuts at: 565 565
Size: 3527
Acc65I G'GTAC_C
Cuts at: 543 543
Size: 3527
AclI AA'CG_TT
Cuts at: 3367 3367
Size: 3527
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1352 1784 2531 2799 394
Size: 958 432 747 268 1122
Fragments arranged by size:
1122 958 747 432 268
AfeI AGC'GCT
Cuts at: 274 274
Size: 3527
AflIII A'CryG_T
Cuts at: 879 1983 2960 879
Size: 1104 977 1446
Fragments arranged by size:
1446 1104 977
AgeI A'CCGG_T
Cuts at: 1915 1915
Size: 3527
AlwNI CAG_nnn'CTG
Cuts at: 426 832 2399 426
Size: 406 1567 1554
Fragments arranged by size:
1567 1554 406
ApaLI G'TGCA_C
Cuts at: 256 351 2297 256
Size: 95 1946 1486
Fragments arranged by size:
1946 1486 95
ApoI r'AATT_y
Cuts at: 531 2880 3136 531
Size: 2349 256 922
Fragments arranged by size:
2349 922 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3527
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3527
AvaI C'yCGr_G
Cuts at: 547 547
Size: 3527
BamHI G'GATC_C
Cuts at: 552 552
Size: 3527
BanI G'GyrC_C
Cuts at: 543 957 1205 1240 543
Size: 414 248 35 2830
Fragments arranged by size:
2830 414 248 35
BanII G_rGCy'C
Cuts at: 541 1571 541
Size: 1030 2497
BbeI G_GCGC'C
Cuts at: 961 1209 961
Size: 248 3279
BciVI GTATCCnnnnn_n'
Cuts at: 1425 2186 3527 1425
Size: 761 1341 1425
Fragments arranged by size:
1425 1341 761
BclI T'GATC_A
Cuts at: 1047 1047
Size: 3527
BfrBI ATG'CAT
Cuts at: 360 360
Size: 3527
BglII A'GATC_T
Cuts at: 1042 3079 1042
Size: 2037 1490
BlpI GC'TnA_GC
Cuts at: 113 639 113
Size: 526 3001
Bme1580I G_kGCm'C
Cuts at: 260 355 1152 1245 2301 260
Size: 95 797 93 1056 1486
Fragments arranged by size:
1486 1056 797 95 93
BmrI ACTGGGnnnn_n'
Cuts at: 739 820 1155 3299 739
Size: 81 335 2144 967
Fragments arranged by size:
2144 967 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1894 1894
Size: 3527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 343
Size: 3527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 703 1582 2074 2372 278
Size: 425 879 492 298 1433
Fragments arranged by size:
1433 879 492 425 298
BsaI GGTCTCn'nnnn_
Cuts at: 202 3231 202
Size: 3029 498
BsaAI yAC'GTr
Cuts at: 880 880
Size: 3527
BsaBI GATnn'nnATC
Cuts at: 6 1041 3078 6
Size: 1035 2037 455
Fragments arranged by size:
2037 1035 455
BsaHI Gr'CG_yC
Cuts at: 958 1206 1908 3404 958
Size: 248 702 1496 1081
Fragments arranged by size:
1496 1081 702 248
BsaWI w'CCGG_w
Cuts at: 182 206 716 1237 1915 2189 2336 2747 182
Size: 24 510 521 678 274 147 411 962
Fragments arranged by size:
962 678 521 510 411 274 147 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 174 174
Size: 3527
BseYI C'CCAG_C
Cuts at: 394 952 1710 2287 2783 394
Size: 558 758 577 496 1138
Fragments arranged by size:
1138 758 577 558 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 231 154
Size: 77 3450
BsiEI CG_ry'CG
Cuts at: 104 1069 1115 2323 104
Size: 965 46 1208 1308
Fragments arranged by size:
1308 1208 965 46
BsiHKAI G_wGCw'C
Cuts at: 260 355 541 1319 1509 2301 260
Size: 95 186 778 190 792 1486
Fragments arranged by size:
1486 792 778 190 186 95
Bsp1286I G_dGCh'C
Cuts at: 260 355 541 1152 1245 1319 1509 1571 2301
Size: 95 186 611 93 74 190 62 730
Cuts at: 2301 260
Size: 1486
Fragments arranged by size:
1486 730 611 190 186 95 93 74
62
BspEI T'CCGG_A
Cuts at: 182 2747 182
Size: 2565 962
BspHI T'CATG_A
Cuts at: 2703 2703
Size: 3527
BspMI ACCTGCnnnn'nnnn_
Cuts at: 243 577 791 1093 1474 243
Size: 334 214 302 381 2296
Fragments arranged by size:
2296 381 334 302 214
BsrBI CCG'CTC
Cuts at: 50 1819 1873 3357 3522 50
Size: 1769 54 1484 165 55
Fragments arranged by size:
1769 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 360 372 1439 360
Size: 12 1067 2448
Fragments arranged by size:
2448 1067 12
BsrFI r'CCGG_y
Cuts at: 1525 1706 1915 1525
Size: 181 209 3137
Fragments arranged by size:
3137 209 181
BssSI C'ACGA_G
Cuts at: 1798 2156 1798
Size: 358 3169
BstAPI GCAn_nnn'nTGC
Cuts at: 904 904
Size: 3527
BstBI TT'CG_AA
Cuts at: 1888 1888
Size: 3527
BstEII G'GTnAC_C
Cuts at: 309 309
Size: 3527
BstXI CCAn_nnnn'nTGG
Cuts at: 321 450 321
Size: 129 3398
BstYI r'GATC_y
Cuts at: 1 552 1019 1042 1377 1623 2635 2721 2733
Size: 551 467 23 335 246 1012 86 12
Cuts at: 2733 3079 1
Size: 346 449
Fragments arranged by size:
1012 551 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 152 107
Size: 45 3482
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1477 1658 1821 1477
Size: 181 163 3183
Fragments arranged by size:
3183 181 163
BtsI GCAGTG_nn'
Cuts at: 770 770
Size: 3527
DraI TTT'AAA
Cuts at: 526 526
Size: 3527
DrdI GACnn_nn'nnGTC
Cuts at: 1233 2091 1233
Size: 858 2669
EaeI y'GGCC_r
Cuts at: 1112 1286 1677 1704 3049 1112
Size: 174 391 27 1345 1590
Fragments arranged by size:
1590 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1112 1112
Size: 3527
EarI CTCTTCn'nnn_
Cuts at: 1550 1760 1550
Size: 210 3317
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1735 2045 2191 3337 1735
Size: 310 146 1146 1925
Fragments arranged by size:
1925 1146 310 146
EcoICRI GAG'CTC
Cuts at: 539 539
Size: 3527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 394 1352 1784 2531 2799 343
Size: 51 958 432 747 268 1071
Fragments arranged by size:
1071 958 747 432 268 51
EcoO109I rG'GnC_Cy
Cuts at: 666 666
Size: 3527
EcoRI G'AATT_C
Cuts at: 531 531
Size: 3527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1546 1546
Size: 3527
FspI TGC'GCA
Cuts at: 1308 1308
Size: 3527
HaeII r_GCGC'y
Cuts at: 218 276 961 1209 2231 3210 218
Size: 58 685 248 1022 979 535
Fragments arranged by size:
1022 979 685 535 248 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 164 180 196 212 1369 1399 1401 1431 164
Size: 16 16 16 1157 30 2 30 2260
Fragments arranged by size:
2260 1157 30 30 16 16 16 2
HincII GTy'rAC
Cuts at: 566 566
Size: 3527
KasI G'GCGC_C
Cuts at: 957 1205 957
Size: 248 3279
KpnI G_GTAC'C
Cuts at: 547 547
Size: 3527
MfeI C'AATT_G
Cuts at: 512 512
Size: 3527
MluI A'CGCG_T
Cuts at: 2960 2960
Size: 3527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2198 2382 2198
Size: 184 3343
MscI TGG'CCA
Cuts at: 1288 1288
Size: 3527
MslI CAynn'nnrTG
Cuts at: 365 1516 1643 365
Size: 1151 127 2249
Fragments arranged by size:
2249 1151 127
MspA1I CmG'CkG
Cuts at: 221 426 638 952 1312 2325 2570 221
Size: 205 212 314 360 1013 245 1178
Fragments arranged by size:
1178 1013 360 314 245 212 205
NaeI GCC'GGC
Cuts at: 1708 1708
Size: 3527
NarI GG'CG_CC
Cuts at: 958 1206 958
Size: 248 3279
NcoI C'CATG_G
Cuts at: 107 152 107
Size: 45 3482
NgoMIV G'CCGG_C
Cuts at: 1706 1706
Size: 3527
NsiI A_TGCA'T
Cuts at: 362 362
Size: 3527
NspI r_CATG'y
Cuts at: 465 580 1080 1987 465
Size: 115 500 907 2005
Fragments arranged by size:
2005 907 500 115
PciI A'CATG_T
Cuts at: 1983 1983
Size: 3527
PflMI CCAn_nnn'nTGG
Cuts at: 306 306
Size: 3527
PfoI T'CCnGG_A
Cuts at: 332 332
Size: 3527
PmeI GTTT'AAAC
Cuts at: 526 526
Size: 3527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2666 2698 2666
Size: 32 3495
PstI C_TGCA'G
Cuts at: 122 574 1259 122
Size: 452 685 2390
Fragments arranged by size:
2390 685 452
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3527
PvuII CAG'CTG
Cuts at: 221 426 952 1312 221
Size: 205 526 360 2436
Fragments arranged by size:
2436 526 360 205
RsrII CG'GwC_CG
Cuts at: 1722 1722
Size: 3527
SacI G_AGCT'C
Cuts at: 541 541
Size: 3527
SalI G'TCGA_C
Cuts at: 564 564
Size: 3527
SapI GCTCTTCn'nnn_
Cuts at: 1550 1760 1550
Size: 210 3317
SbfI CC_TGCA'GG
Cuts at: 574 574
Size: 3527
ScaI AGT'ACT
Cuts at: 3044 3044
Size: 3527
SfcI C'TryA_G
Cuts at: 33 118 570 1255 2248 2439 33
Size: 85 452 685 993 191 1121
Fragments arranged by size:
1121 993 685 452 191 85
SfoI GGC'GCC
Cuts at: 959 1207 959
Size: 248 3279
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3527
SmaI CCC'GGG
Cuts at: 549 549
Size: 3527
SmlI C'TyrA_G
Cuts at: 293 682 1597 2089 2351 293
Size: 389 915 492 262 1469
Fragments arranged by size:
1469 915 492 389 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3527
SphI G_CATG'C
Cuts at: 580 1080 580
Size: 500 3027
StuI AGG'CCT
Cuts at: 241 241
Size: 3527
StyI C'CwwG_G
Cuts at: 107 152 661 1015 107
Size: 45 509 354 2619
Fragments arranged by size:
2619 509 354 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1110 1243 1702 1911 1110
Size: 133 459 209 2726
Fragments arranged by size:
2726 459 209 133
TatI w'GTAC_w
Cuts at: 3042 3042
Size: 3527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 3495
Tth111I GACn'n_nGTC
Cuts at: 1324 1324
Size: 3527
XbaI T'CTAG_A
Cuts at: 558 558
Size: 3527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1022 1022
Size: 3527
XmaI C'CCGG_G
Cuts at: 547 547
Size: 3527
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI