Restriction map for
pF1KE0163
(Circular) (Six-base) MAPSORT of: pF1KE0163.seq Check: 9675 from: 1 to: 3452
pF1KE0163 3452 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 490 490
Size: 3452
Acc65I G'GTAC_C
Cuts at: 215 468 215
Size: 253 3199
AclI AA'CG_TT
Cuts at: 3292 3292
Size: 3452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 1709 2456 2724 1277
Size: 432 747 268 2005
Fragments arranged by size:
2005 747 432 268
AfeI AGC'GCT
Cuts at: 373 373
Size: 3452
AflIII A'CryG_T
Cuts at: 333 804 1908 2885 333
Size: 471 1104 977 900
Fragments arranged by size:
1104 977 900 471
AgeI A'CCGG_T
Cuts at: 1840 1840
Size: 3452
AlwNI CAG_nnn'CTG
Cuts at: 757 2324 757
Size: 1567 1885
ApaLI G'TGCA_C
Cuts at: 236 2222 236
Size: 1986 1466
ApoI r'AATT_y
Cuts at: 352 456 2805 3061 352
Size: 104 2349 256 743
Fragments arranged by size:
2349 743 256 104
AseI AT'TA_AT
Cuts at: 20 20
Size: 3452
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3452
AvaI C'yCGr_G
Cuts at: 472 472
Size: 3452
BamHI G'GATC_C
Cuts at: 477 477
Size: 3452
BanI G'GyrC_C
Cuts at: 215 468 882 1130 1165 215
Size: 253 414 248 35 2502
Fragments arranged by size:
2502 414 253 248 35
BanII G_rGCy'C
Cuts at: 273 466 1496 273
Size: 193 1030 2229
Fragments arranged by size:
2229 1030 193
BbeI G_GCGC'C
Cuts at: 886 1134 886
Size: 248 3204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 121 155 121
Size: 34 3418
BciVI GTATCCnnnnn_n'
Cuts at: 1350 2111 3452 1350
Size: 761 1341 1350
Fragments arranged by size:
1350 1341 761
BclI T'GATC_A
Cuts at: 972 972
Size: 3452
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3452
BglII A'GATC_T
Cuts at: 967 3004 967
Size: 2037 1415
BlpI GC'TnA_GC
Cuts at: 564 564
Size: 3452
Bme1580I G_kGCm'C
Cuts at: 240 1077 1170 2226 240
Size: 837 93 1056 1466
Fragments arranged by size:
1466 1056 837 93
BmrI ACTGGGnnnn_n'
Cuts at: 664 745 1080 3224 664
Size: 81 335 2144 892
Fragments arranged by size:
2144 892 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1819 1819
Size: 3452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 446
Size: 3452
Bpu10I CC'TnA_GC
Cuts at: 169 169
Size: 3452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 628 1507 1999 2297 391
Size: 237 879 492 298 1546
Fragments arranged by size:
1546 879 492 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 409 3156 409
Size: 2747 705
BsaAI yAC'GTr
Cuts at: 805 805
Size: 3452
BsaBI GATnn'nnATC
Cuts at: 6 966 3003 6
Size: 960 2037 455
Fragments arranged by size:
2037 960 455
BsaHI Gr'CG_yC
Cuts at: 883 1131 1833 3329 883
Size: 248 702 1496 1006
Fragments arranged by size:
1496 1006 702 248
BsaWI w'CCGG_w
Cuts at: 326 641 1162 1840 2114 2261 2672 326
Size: 315 521 678 274 147 411 1106
Fragments arranged by size:
1106 678 521 411 315 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 397 397
Size: 3452
BseYI C'CCAG_C
Cuts at: 437 877 1635 2212 2708 437
Size: 440 758 577 496 1181
Fragments arranged by size:
1181 758 577 496 440
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 285
Size: 3452
BsiEI CG_ry'CG
Cuts at: 104 186 994 1040 2248 104
Size: 82 808 46 1208 1308
Fragments arranged by size:
1308 1208 808 82 46
BsiHKAI G_wGCw'C
Cuts at: 240 466 1244 1434 2226 240
Size: 226 778 190 792 1466
Fragments arranged by size:
1466 792 778 226 190
Bsp1286I G_dGCh'C
Cuts at: 240 273 466 1077 1170 1244 1434 1496 2226
Size: 33 193 611 93 74 190 62 730
Cuts at: 2226 240
Size: 1466
Fragments arranged by size:
1466 730 611 193 190 93 74 62
33
BspEI T'CCGG_A
Cuts at: 326 2672 326
Size: 2346 1106
BspHI T'CATG_A
Cuts at: 2628 2628
Size: 3452
BspMI ACCTGCnnnn'nnnn_
Cuts at: 502 716 1018 1399 502
Size: 214 302 381 2555
Fragments arranged by size:
2555 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1744 1798 3282 3447 50
Size: 1694 54 1484 165 55
Fragments arranged by size:
1694 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1364 1364
Size: 3452
BsrFI r'CCGG_y
Cuts at: 181 1450 1631 1840 181
Size: 1269 181 209 1793
Fragments arranged by size:
1793 1269 209 181
BssSI C'ACGA_G
Cuts at: 1723 2081 1723
Size: 358 3094
BstAPI GCAn_nnn'nTGC
Cuts at: 829 829
Size: 3452
BstBI TT'CG_AA
Cuts at: 1813 1813
Size: 3452
BstEII G'GTnAC_C
Cuts at: 246 246
Size: 3452
BstXI CCAn_nnnn'nTGG
Cuts at: 424 424
Size: 3452
BstYI r'GATC_y
Cuts at: 1 477 944 967 1302 1548 2560 2646 2658
Size: 476 467 23 335 246 1012 86 12
Cuts at: 2658 3004 1
Size: 346 449
Fragments arranged by size:
1012 476 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 117 117
Size: 3452
BtgI C'CryG_G
Cuts at: 107 203 107
Size: 96 3356
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1402 1583 1746 1402
Size: 181 163 3108
Fragments arranged by size:
3108 181 163
BtsI GCAGTG_nn'
Cuts at: 695 695
Size: 3452
DraI TTT'AAA
Cuts at: 451 451
Size: 3452
DrdI GACnn_nn'nnGTC
Cuts at: 1158 2016 1158
Size: 858 2594
EaeI y'GGCC_r
Cuts at: 176 183 1037 1211 1602 1629 2974 176
Size: 7 854 174 391 27 1345 654
Fragments arranged by size:
1345 854 654 391 174 27 7
EagI C'GGCC_G
Cuts at: 183 1037 183
Size: 854 2598
EarI CTCTTCn'nnn_
Cuts at: 1475 1685 1475
Size: 210 3242
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1660 1970 2116 3262 1660
Size: 310 146 1146 1850
Fragments arranged by size:
1850 1146 310 146
EcoICRI GAG'CTC
Cuts at: 464 464
Size: 3452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 1277 1709 2456 2724 446
Size: 831 432 747 268 1174
Fragments arranged by size:
1174 831 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 223 346 223
Size: 123 3329
EcoO109I rG'GnC_Cy
Cuts at: 122 591 122
Size: 469 2983
EcoRI G'AATT_C
Cuts at: 456 456
Size: 3452
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1471 1471
Size: 3452
FspI TGC'GCA
Cuts at: 1233 1233
Size: 3452
HaeII r_GCGC'y
Cuts at: 375 886 1134 2156 3135 375
Size: 511 248 1022 979 692
Fragments arranged by size:
1022 979 692 511 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1294 1324 1326 1356 1294
Size: 30 2 30 3390
Fragments arranged by size:
3390 30 30 2
HincII GTy'rAC
Cuts at: 491 491
Size: 3452
KasI G'GCGC_C
Cuts at: 882 1130 882
Size: 248 3204
KpnI G_GTAC'C
Cuts at: 219 472 219
Size: 253 3199
MluI A'CGCG_T
Cuts at: 2885 2885
Size: 3452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2123 2307 2123
Size: 184 3268
MscI TGG'CCA
Cuts at: 1213 1213
Size: 3452
MslI CAynn'nnrTG
Cuts at: 1441 1568 1441
Size: 127 3325
MspA1I CmG'CkG
Cuts at: 205 563 877 1237 2250 2495 205
Size: 358 314 360 1013 245 1162
Fragments arranged by size:
1162 1013 360 358 314 245
NaeI GCC'GGC
Cuts at: 1633 1633
Size: 3452
NarI GG'CG_CC
Cuts at: 883 1131 883
Size: 248 3204
NcoI C'CATG_G
Cuts at: 107 107
Size: 3452
NgoMIV G'CCGG_C
Cuts at: 1631 1631
Size: 3452
NruI TCG'CGA
Cuts at: 197 197
Size: 3452
NspI r_CATG'y
Cuts at: 337 505 1005 1912 337
Size: 168 500 907 1877
Fragments arranged by size:
1877 907 500 168
PciI A'CATG_T
Cuts at: 333 1908 333
Size: 1575 1877
PmeI GTTT'AAAC
Cuts at: 451 451
Size: 3452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2591 2623 2591
Size: 32 3420
PpuMI rG'GwC_Cy
Cuts at: 122 122
Size: 3452
PstI C_TGCA'G
Cuts at: 499 1184 499
Size: 685 2767
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3452
PvuII CAG'CTG
Cuts at: 877 1237 877
Size: 360 3092
RsrII CG'GwC_CG
Cuts at: 1647 1647
Size: 3452
SacI G_AGCT'C
Cuts at: 466 466
Size: 3452
SacII CC_GC'GG
Cuts at: 206 206
Size: 3452
SalI G'TCGA_C
Cuts at: 489 489
Size: 3452
SanDI GG'GwC_CC
Cuts at: 122 122
Size: 3452
SapI GCTCTTCn'nnn_
Cuts at: 1475 1685 1475
Size: 210 3242
SbfI CC_TGCA'GG
Cuts at: 499 499
Size: 3452
ScaI AGT'ACT
Cuts at: 2969 2969
Size: 3452
SfcI C'TryA_G
Cuts at: 33 495 1180 2173 2364 33
Size: 462 685 993 191 1121
Fragments arranged by size:
1121 993 685 462 191
SfoI GGC'GCC
Cuts at: 884 1132 884
Size: 248 3204
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3452
SmaI CCC'GGG
Cuts at: 474 474
Size: 3452
SmlI C'TyrA_G
Cuts at: 406 607 1522 2014 2276 406
Size: 201 915 492 262 1582
Fragments arranged by size:
1582 915 492 262 201
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3452
SphI G_CATG'C
Cuts at: 505 1005 505
Size: 500 2952
StyI C'CwwG_G
Cuts at: 107 586 940 107
Size: 479 354 2619
Fragments arranged by size:
2619 479 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1035 1168 1627 1836 1035
Size: 133 459 209 2651
Fragments arranged by size:
2651 459 209 133
TatI w'GTAC_w
Cuts at: 2967 2967
Size: 3452
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 3420
Tth111I GACn'n_nGTC
Cuts at: 1249 1249
Size: 3452
XbaI T'CTAG_A
Cuts at: 483 483
Size: 3452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 947 947
Size: 3452
XmaI C'CCGG_G
Cuts at: 472 472
Size: 3452
XmnI GAAnn'nnTTC
Cuts at: 229 229
Size: 3452
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI