Restriction map for
pF1KE0162
(Circular) (Six-base) MAPSORT of: pF1KE0162.seq Check: 9131 from: 1 to: 4043
pF1KE0162 4043 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 1081 1081
Size: 4043
Acc65I G'GTAC_C
Cuts at: 1059 1059
Size: 4043
AclI AA'CG_TT
Cuts at: 3883 3883
Size: 4043
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 300 1022 1868 2300 3047 3315 184
Size: 116 722 846 432 747 268 912
Fragments arranged by size:
912 846 747 722 432 268 116
AflIII A'CryG_T
Cuts at: 1395 2499 3476 1395
Size: 1104 977 1962
Fragments arranged by size:
1962 1104 977
AgeI A'CCGG_T
Cuts at: 2431 2431
Size: 4043
AhdI GACnn_n'nnGTC
Cuts at: 763 763
Size: 4043
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 981 1013 981
Size: 32 4011
AlwNI CAG_nnn'CTG
Cuts at: 283 360 682 922 1348 2915 283
Size: 77 322 240 426 1567 1411
Fragments arranged by size:
1567 1411 426 322 240 77
ApaLI G'TGCA_C
Cuts at: 2813 2813
Size: 4043
ApoI r'AATT_y
Cuts at: 925 1047 3396 3652 925
Size: 122 2349 256 1316
Fragments arranged by size:
2349 1316 256 122
AseI AT'TA_AT
Cuts at: 20 20
Size: 4043
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
AvaI C'yCGr_G
Cuts at: 395 1063 395
Size: 668 3375
BamHI G'GATC_C
Cuts at: 789 1068 789
Size: 279 3764
BanI G'GyrC_C
Cuts at: 458 556 1059 1473 1721 1756 458
Size: 98 503 414 248 35 2745
Fragments arranged by size:
2745 503 414 248 98 35
BanII G_rGCy'C
Cuts at: 449 824 1057 2087 449
Size: 375 233 1030 2405
Fragments arranged by size:
2405 1030 375 233
BbeI G_GCGC'C
Cuts at: 1477 1725 1477
Size: 248 3795
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4009
BciVI GTATCCnnnnn_n'
Cuts at: 1941 2702 4043 1941
Size: 761 1341 1941
Fragments arranged by size:
1941 1341 761
BclI T'GATC_A
Cuts at: 1563 1563
Size: 4043
BglII A'GATC_T
Cuts at: 1558 3595 1558
Size: 2037 2006
BlpI GC'TnA_GC
Cuts at: 1155 1155
Size: 4043
Bme1580I G_kGCm'C
Cuts at: 461 856 1668 1761 2817 461
Size: 395 812 93 1056 1687
Fragments arranged by size:
1687 1056 812 395 93
BmrI ACTGGGnnnn_n'
Cuts at: 700 1255 1336 1671 3815 700
Size: 555 81 335 2144 928
Fragments arranged by size:
2144 928 555 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 155 347 592 659 823 103
Size: 52 192 245 67 164 3323
Fragments arranged by size:
3323 245 192 164 67 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 1219 2098 2590 2888 674
Size: 545 879 492 298 1829
Fragments arranged by size:
1829 879 545 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 475 931 3747 475
Size: 456 2816 771
Fragments arranged by size:
2816 771 456
BsaAI yAC'GTr
Cuts at: 1396 1396
Size: 4043
BsaBI GATnn'nnATC
Cuts at: 6 1557 3594 6
Size: 1551 2037 455
Fragments arranged by size:
2037 1551 455
BsaHI Gr'CG_yC
Cuts at: 1474 1722 2424 3920 1474
Size: 248 702 1496 1597
Fragments arranged by size:
1597 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1232 1753 2431 2705 2852 3263 1232
Size: 521 678 274 147 411 2012
Fragments arranged by size:
2012 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 272 302 272
Size: 30 4013
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 335 820 335
Size: 485 3558
BseYI C'CCAG_C
Cuts at: 146 489 873 945 1468 2226 2803 3299 146
Size: 343 384 72 523 758 577 496 890
Fragments arranged by size:
890 758 577 523 496 384 343 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 526 314
Size: 212 3831
BsiEI CG_ry'CG
Cuts at: 104 1585 1631 2839 104
Size: 1481 46 1208 1308
Fragments arranged by size:
1481 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 675 824 1057 1835 2025 2817 675
Size: 149 233 778 190 792 1901
Fragments arranged by size:
1901 792 778 233 190 149
Bsp1286I G_dGCh'C
Cuts at: 449 461 675 824 856 1057 1668 1761 1835
Size: 12 214 149 32 201 611 93 74
Cuts at: 1835 2025 2087 2817 449
Size: 190 62 730 1675
Fragments arranged by size:
1675 730 611 214 201 190 149 93
74 62 32 12
BspEI T'CCGG_A
Cuts at: 3263 3263
Size: 4043
BspHI T'CATG_A
Cuts at: 1017 3219 1017
Size: 2202 1841
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1093 1307 1609 1990 1093
Size: 214 302 381 3146
Fragments arranged by size:
3146 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2335 2389 3873 4038 50
Size: 2285 54 1484 165 55
Fragments arranged by size:
2285 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1955 1955
Size: 4043
BsrFI r'CCGG_y
Cuts at: 419 503 2041 2222 2431 419
Size: 84 1538 181 209 2031
Fragments arranged by size:
2031 1538 209 181 84
BssSI C'ACGA_G
Cuts at: 2314 2672 2314
Size: 358 3685
BstAPI GCAn_nnn'nTGC
Cuts at: 1420 1420
Size: 4043
BstBI TT'CG_AA
Cuts at: 237 2404 237
Size: 2167 1876
BstYI r'GATC_y
Cuts at: 1 575 789 1068 1535 1558 1893 2139 3151
Size: 574 214 279 467 23 335 246 1012
Cuts at: 3151 3237 3249 3595 1
Size: 86 12 346 449
Fragments arranged by size:
1012 574 467 449 346 335 279 246
214 86 23 12
BtgI C'CryG_G
Cuts at: 579 579
Size: 4043
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1993 2174 2337 1993
Size: 181 163 3699
Fragments arranged by size:
3699 181 163
BtsI GCAGTG_nn'
Cuts at: 1286 1286
Size: 4043
ClaI AT'CG_AT
Cuts at: 533 533
Size: 4043
DraI TTT'AAA
Cuts at: 594 1042 594
Size: 448 3595
DrdI GACnn_nn'nnGTC
Cuts at: 1749 2607 1749
Size: 858 3185
EaeI y'GGCC_r
Cuts at: 211 1628 1802 2193 2220 3565 211
Size: 1417 174 391 27 1345 689
Fragments arranged by size:
1417 1345 689 391 174 27
EagI C'GGCC_G
Cuts at: 1628 1628
Size: 4043
EarI CTCTTCn'nnn_
Cuts at: 725 1001 2066 2276 725
Size: 276 1065 210 2492
Fragments arranged by size:
2492 1065 276 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2251 2561 2707 3853 2251
Size: 310 146 1146 2441
Fragments arranged by size:
2441 1146 310 146
EcoICRI GAG'CTC
Cuts at: 822 1055 822
Size: 233 3810
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 155 184 300 347 592 659 823 1022
Size: 52 29 116 47 245 67 164 199
Cuts at: 1022 1868 2300 3047 3315 103
Size: 846 432 747 268 831
Fragments arranged by size:
846 831 747 432 268 245 199 164
116 67 52 47 29
EcoO109I rG'GnC_Cy
Cuts at: 357 979 1182 357
Size: 622 203 3218
Fragments arranged by size:
3218 622 203
EcoRI G'AATT_C
Cuts at: 1047 1047
Size: 4043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 4043
FspI TGC'GCA
Cuts at: 1824 1824
Size: 4043
HaeII r_GCGC'y
Cuts at: 1477 1725 2747 3726 1477
Size: 248 1022 979 1794
Fragments arranged by size:
1794 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 500 532 749 781 1885 1915 1917 1947 500
Size: 32 217 32 1104 30 2 30 2596
Fragments arranged by size:
2596 1104 217 32 32 30 30 2
HincII GTy'rAC
Cuts at: 813 1082 813
Size: 269 3774
KasI G'GCGC_C
Cuts at: 1473 1721 1473
Size: 248 3795
KpnI G_GTAC'C
Cuts at: 1063 1063
Size: 4043
MluI A'CGCG_T
Cuts at: 3476 3476
Size: 4043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 334 2714 2898 334
Size: 2380 184 1479
Fragments arranged by size:
2380 1479 184
MscI TGG'CCA
Cuts at: 1804 1804
Size: 4043
MslI CAynn'nnrTG
Cuts at: 1022 2032 2159 1022
Size: 1010 127 2906
Fragments arranged by size:
2906 1010 127
MspA1I CmG'CkG
Cuts at: 1154 1468 1828 2841 3086 1154
Size: 314 360 1013 245 2111
Fragments arranged by size:
2111 1013 360 314 245
NaeI GCC'GGC
Cuts at: 505 2224 505
Size: 1719 2324
NarI GG'CG_CC
Cuts at: 1474 1722 1474
Size: 248 3795
NcoI C'CATG_G
Cuts at: 579 579
Size: 4043
NgoMIV G'CCGG_C
Cuts at: 503 2222 503
Size: 1719 2324
NspI r_CATG'y
Cuts at: 1096 1596 2503 1096
Size: 500 907 2636
Fragments arranged by size:
2636 907 500
PciI A'CATG_T
Cuts at: 2499 2499
Size: 4043
PflMI CCAn_nnn'nTGG
Cuts at: 360 682 360
Size: 322 3721
PmeI GTTT'AAAC
Cuts at: 1042 1042
Size: 4043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 4011
PpuMI rG'GwC_Cy
Cuts at: 357 979 357
Size: 622 3421
PstI C_TGCA'G
Cuts at: 1090 1775 1090
Size: 685 3358
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4043
PvuII CAG'CTG
Cuts at: 1468 1828 1468
Size: 360 3683
RsrII CG'GwC_CG
Cuts at: 2238 2238
Size: 4043
SacI G_AGCT'C
Cuts at: 824 1057 824
Size: 233 3810
SalI G'TCGA_C
Cuts at: 1080 1080
Size: 4043
SanDI GG'GwC_CC
Cuts at: 979 979
Size: 4043
SapI GCTCTTCn'nnn_
Cuts at: 2066 2276 2066
Size: 210 3833
SbfI CC_TGCA'GG
Cuts at: 1090 1090
Size: 4043
ScaI AGT'ACT
Cuts at: 3560 3560
Size: 4043
SfcI C'TryA_G
Cuts at: 33 1086 1771 2764 2955 33
Size: 1053 685 993 191 1121
Fragments arranged by size:
1121 1053 993 685 191
SfoI GGC'GCC
Cuts at: 1475 1723 1475
Size: 248 3795
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
SmaI CCC'GGG
Cuts at: 397 1065 397
Size: 668 3375
SmlI C'TyrA_G
Cuts at: 689 1198 2113 2605 2867 689
Size: 509 915 492 262 1865
Fragments arranged by size:
1865 915 509 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4043
SphI G_CATG'C
Cuts at: 1096 1596 1096
Size: 500 3543
StyI C'CwwG_G
Cuts at: 579 1177 1531 579
Size: 598 354 3091
Fragments arranged by size:
3091 598 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 538 1626 1759 2218 2427 538
Size: 1088 133 459 209 2154
Fragments arranged by size:
2154 1088 459 209 133
TatI w'GTAC_w
Cuts at: 3558 3558
Size: 4043
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 376 408 3560 3592 376
Size: 32 3152 32 827
Fragments arranged by size:
3152 827 32 32
Tth111I GACn'n_nGTC
Cuts at: 1840 1840
Size: 4043
XbaI T'CTAG_A
Cuts at: 1074 1074
Size: 4043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 154 567 982 1538 154
Size: 413 415 556 2659
Fragments arranged by size:
2659 556 415 413
XmaI C'CCGG_G
Cuts at: 395 1063 395
Size: 668 3375
XmnI GAAnn'nnTTC
Cuts at: 958 958
Size: 4043
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI