Restriction map for
pF1KE0150
(Circular) (Six-base) MAPSORT of: pF1KE0150.seq Check: 3545 from: 1 to: 4058
pF1KE0150 4058 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 243 243
Size: 4058
AccI GT'mk_AC
Cuts at: 1096 1096
Size: 4058
Acc65I G'GTAC_C
Cuts at: 1074 1074
Size: 4058
AclI AA'CG_TT
Cuts at: 3898 3898
Size: 4058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 1883 2315 3062 3330 330
Size: 1553 432 747 268 1058
Fragments arranged by size:
1553 1058 747 432 268
AflIII A'CryG_T
Cuts at: 1410 2514 3491 1410
Size: 1104 977 1977
Fragments arranged by size:
1977 1104 977
AgeI A'CCGG_T
Cuts at: 2446 2446
Size: 4058
AlwNI CAG_nnn'CTG
Cuts at: 1363 2930 1363
Size: 1567 2491
ApaLI G'TGCA_C
Cuts at: 2828 2828
Size: 4058
ApoI r'AATT_y
Cuts at: 1024 1062 3411 3667 1024
Size: 38 2349 256 1415
Fragments arranged by size:
2349 1415 256 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 4058
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
AvaI C'yCGr_G
Cuts at: 283 1078 283
Size: 795 3263
BamHI G'GATC_C
Cuts at: 1083 1083
Size: 4058
BanI G'GyrC_C
Cuts at: 222 955 1074 1488 1736 1771 222
Size: 733 119 414 248 35 2509
Fragments arranged by size:
2509 733 414 248 119 35
BanII G_rGCy'C
Cuts at: 1072 2102 1072
Size: 1030 3028
BbeI G_GCGC'C
Cuts at: 226 959 1492 1740 226
Size: 733 533 248 2544
Fragments arranged by size:
2544 733 533 248
BbsI GAAGACnn'nnnn_
Cuts at: 855 855
Size: 4058
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4024
BciVI GTATCCnnnnn_n'
Cuts at: 1956 2717 4058 1956
Size: 761 1341 1956
Fragments arranged by size:
1956 1341 761
BclI T'GATC_A
Cuts at: 1578 1578
Size: 4058
BfrBI ATG'CAT
Cuts at: 111 111
Size: 4058
BglII A'GATC_T
Cuts at: 1573 3610 1573
Size: 2037 2021
BlpI GC'TnA_GC
Cuts at: 1170 1170
Size: 4058
Bme1580I G_kGCm'C
Cuts at: 1683 1776 2832 1683
Size: 93 1056 2909
Fragments arranged by size:
2909 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1270 1351 1686 3830 1270
Size: 81 335 2144 1498
Fragments arranged by size:
2144 1498 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 467 492 467
Size: 25 4033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 779 1234 2113 2605 2903 779
Size: 455 879 492 298 1934
Fragments arranged by size:
1934 879 492 455 298
BsaI GGTCTCn'nnnn_
Cuts at: 3762 3762
Size: 4058
BsaAI yAC'GTr
Cuts at: 1411 1411
Size: 4058
BsaBI GATnn'nnATC
Cuts at: 6 1572 3609 6
Size: 1566 2037 455
Fragments arranged by size:
2037 1566 455
BsaHI Gr'CG_yC
Cuts at: 155 223 262 956 1489 1737 2439 3935 155
Size: 68 39 694 533 248 702 1496 278
Fragments arranged by size:
1496 702 694 533 278 248 68 39
BsaWI w'CCGG_w
Cuts at: 1247 1768 2446 2720 2867 3278 1247
Size: 521 678 274 147 411 2027
Fragments arranged by size:
2027 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 607 607
Size: 4058
BseYI C'CCAG_C
Cuts at: 511 1483 2241 2818 3314 511
Size: 972 758 577 496 1255
Fragments arranged by size:
1255 972 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 277
Size: 4058
BsiEI CG_ry'CG
Cuts at: 104 1600 1646 2854 104
Size: 1496 46 1208 1308
Fragments arranged by size:
1496 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1072 1850 2040 2832 1072
Size: 778 190 792 2298
Fragments arranged by size:
2298 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1072 1683 1776 1850 2040 2102 2832 1072
Size: 611 93 74 190 62 730 2298
Fragments arranged by size:
2298 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3278 3278
Size: 4058
BspHI T'CATG_A
Cuts at: 3234 3234
Size: 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 243 1108 1322 1624 2005 243
Size: 865 214 302 381 2296
Fragments arranged by size:
2296 865 381 302 214
BsrBI CCG'CTC
Cuts at: 50 234 2350 2404 3888 4053 50
Size: 184 2116 54 1484 165 55
Fragments arranged by size:
2116 1484 184 165 55 54
BsrDI GCAATG_nn'
Cuts at: 788 1970 788
Size: 1182 2876
BsrFI r'CCGG_y
Cuts at: 184 2056 2237 2446 184
Size: 1872 181 209 1796
Fragments arranged by size:
1872 1796 209 181
BssSI C'ACGA_G
Cuts at: 2329 2687 2329
Size: 358 3700
BstAPI GCAn_nnn'nTGC
Cuts at: 1435 1435
Size: 4058
BstBI TT'CG_AA
Cuts at: 2419 2419
Size: 4058
BstXI CCAn_nnnn'nTGG
Cuts at: 673 673
Size: 4058
BstYI r'GATC_y
Cuts at: 1 1083 1550 1573 1908 2154 3166 3252 3264
Size: 1082 467 23 335 246 1012 86 12
Cuts at: 3264 3610 1
Size: 346 449
Fragments arranged by size:
1082 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 590 590
Size: 4058
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2008 2189 2352 2008
Size: 181 163 3714
Fragments arranged by size:
3714 181 163
BtsI GCAGTG_nn'
Cuts at: 1301 1301
Size: 4058
DraI TTT'AAA
Cuts at: 1057 1057
Size: 4058
DrdI GACnn_nn'nnGTC
Cuts at: 1764 2622 1764
Size: 858 3200
EaeI y'GGCC_r
Cuts at: 182 1643 1817 2208 2235 3580 182
Size: 1461 174 391 27 1345 660
Fragments arranged by size:
1461 1345 660 391 174 27
EagI C'GGCC_G
Cuts at: 1643 1643
Size: 4058
EarI CTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 203 2266 2576 2722 3868 203
Size: 2063 310 146 1146 393
Fragments arranged by size:
2063 1146 393 310 146
EcoICRI GAG'CTC
Cuts at: 1070 1070
Size: 4058
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 467 492 1883 2315 3062 3330 330
Size: 137 25 1391 432 747 268 1058
Fragments arranged by size:
1391 1058 747 432 268 137 25
EcoO109I rG'GnC_Cy
Cuts at: 174 586 1197 174
Size: 412 611 3035
Fragments arranged by size:
3035 611 412
EcoRI G'AATT_C
Cuts at: 1024 1062 1024
Size: 38 4020
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 4058
FspI TGC'GCA
Cuts at: 1839 1839
Size: 4058
HaeII r_GCGC'y
Cuts at: 226 959 1492 1740 2762 3741 226
Size: 733 533 248 1022 979 543
Fragments arranged by size:
1022 979 733 543 533 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 145 177 554 586 1900 1930 1932 1962 145
Size: 32 377 32 1314 30 2 30 2241
Fragments arranged by size:
2241 1314 377 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1097 1097
Size: 4058
KasI G'GCGC_C
Cuts at: 222 955 1488 1736 222
Size: 733 533 248 2544
Fragments arranged by size:
2544 733 533 248
KpnI G_GTAC'C
Cuts at: 1078 1078
Size: 4058
MluI A'CGCG_T
Cuts at: 3491 3491
Size: 4058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 415 2729 2913 415
Size: 2314 184 1560
Fragments arranged by size:
2314 1560 184
MscI TGG'CCA
Cuts at: 1819 1819
Size: 4058
MslI CAynn'nnrTG
Cuts at: 525 697 2047 2174 525
Size: 172 1350 127 2409
Fragments arranged by size:
2409 1350 172 127
MspA1I CmG'CkG
Cuts at: 202 217 1169 1483 1843 2856 3101 202
Size: 15 952 314 360 1013 245 1159
Fragments arranged by size:
1159 1013 952 360 314 245 15
NaeI GCC'GGC
Cuts at: 186 2239 186
Size: 2053 2005
NarI GG'CG_CC
Cuts at: 223 956 1489 1737 223
Size: 733 533 248 2544
Fragments arranged by size:
2544 733 533 248
NgoMIV G'CCGG_C
Cuts at: 184 2237 184
Size: 2053 2005
NruI TCG'CGA
Cuts at: 332 332
Size: 4058
NsiI A_TGCA'T
Cuts at: 113 113
Size: 4058
NspI r_CATG'y
Cuts at: 812 1111 1611 2518 812
Size: 299 500 907 2352
Fragments arranged by size:
2352 907 500 299
PciI A'CATG_T
Cuts at: 2514 2514
Size: 4058
PmeI GTTT'AAAC
Cuts at: 1057 1057
Size: 4058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 4026
PpuMI rG'GwC_Cy
Cuts at: 174 586 174
Size: 412 3646
PstI C_TGCA'G
Cuts at: 254 1105 1790 254
Size: 851 685 2522
Fragments arranged by size:
2522 851 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4058
PvuII CAG'CTG
Cuts at: 202 1483 1843 202
Size: 1281 360 2417
Fragments arranged by size:
2417 1281 360
RsrII CG'GwC_CG
Cuts at: 2253 2253
Size: 4058
SacI G_AGCT'C
Cuts at: 1072 1072
Size: 4058
SalI G'TCGA_C
Cuts at: 1095 1095
Size: 4058
SapI GCTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
SbfI CC_TGCA'GG
Cuts at: 1105 1105
Size: 4058
ScaI AGT'ACT
Cuts at: 3575 3575
Size: 4058
SfcI C'TryA_G
Cuts at: 33 250 1101 1786 2779 2970 33
Size: 217 851 685 993 191 1121
Fragments arranged by size:
1121 993 851 685 217 191
SfoI GGC'GCC
Cuts at: 224 957 1490 1738 224
Size: 733 533 248 2544
Fragments arranged by size:
2544 733 533 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
SmaI CCC'GGG
Cuts at: 1080 1080
Size: 4058
SmlI C'TyrA_G
Cuts at: 794 1213 2128 2620 2882 794
Size: 419 915 492 262 1970
Fragments arranged by size:
1970 915 492 419 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4058
SphI G_CATG'C
Cuts at: 1111 1611 1111
Size: 500 3558
StyI C'CwwG_G
Cuts at: 666 1192 1546 666
Size: 526 354 3178
Fragments arranged by size:
3178 526 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1641 1774 2233 2442 1641
Size: 133 459 209 3257
Fragments arranged by size:
3257 459 209 133
TatI w'GTAC_w
Cuts at: 684 3573 684
Size: 2889 1169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3575 3607 3575
Size: 32 4026
Tth111I GACn'n_nGTC
Cuts at: 1855 1855
Size: 4058
XbaI T'CTAG_A
Cuts at: 543 1089 543
Size: 546 3512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 216 1553 216
Size: 1337 2721
XmaI C'CCGG_G
Cuts at: 1078 1078
Size: 4058
XmnI GAAnn'nnTTC
Cuts at: 822 939 1028 822
Size: 117 89 3852
Fragments arranged by size:
3852 117 89
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI
NsiI NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI