Restriction map for
pF1KE0149
(Circular) (Six-base) MAPSORT of: pF1KE0149.seq Check: 8637 from: 1 to: 3581
pF1KE0149 3581 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 619 619
Size: 3581
Acc65I G'GTAC_C
Cuts at: 597 597
Size: 3581
AclI AA'CG_TT
Cuts at: 3421 3421
Size: 3581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1406 1838 2585 2853 1406
Size: 432 747 268 2134
Fragments arranged by size:
2134 747 432 268
AflIII A'CryG_T
Cuts at: 933 2037 3014 933
Size: 1104 977 1500
Fragments arranged by size:
1500 1104 977
AgeI A'CCGG_T
Cuts at: 1969 1969
Size: 3581
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 224 256 224
Size: 32 3549
AlwNI CAG_nnn'CTG
Cuts at: 886 2453 886
Size: 1567 2014
ApaLI G'TGCA_C
Cuts at: 2351 2351
Size: 3581
ApoI r'AATT_y
Cuts at: 585 2934 3190 585
Size: 2349 256 976
Fragments arranged by size:
2349 976 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3581
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3581
AvaI C'yCGr_G
Cuts at: 129 601 129
Size: 472 3109
BamHI G'GATC_C
Cuts at: 606 606
Size: 3581
BanI G'GyrC_C
Cuts at: 297 324 597 1011 1259 1294 297
Size: 27 273 414 248 35 2584
Fragments arranged by size:
2584 414 273 248 35 27
BanII G_rGCy'C
Cuts at: 190 359 595 1625 190
Size: 169 236 1030 2146
Fragments arranged by size:
2146 1030 236 169
BbeI G_GCGC'C
Cuts at: 1015 1263 1015
Size: 248 3333
BbsI GAAGACnn'nnnn_
Cuts at: 214 214
Size: 3581
BbvCI CC'TCA_GC
Cuts at: 362 362
Size: 3581
BciVI GTATCCnnnnn_n'
Cuts at: 1479 2240 3581 1479
Size: 761 1341 1479
Fragments arranged by size:
1479 1341 761
BclI T'GATC_A
Cuts at: 1101 1101
Size: 3581
BglI GCCn_nnn'nGGC
Cuts at: 154 295 154
Size: 141 3440
BglII A'GATC_T
Cuts at: 1096 3133 1096
Size: 2037 1544
BlpI GC'TnA_GC
Cuts at: 693 693
Size: 3581
Bme1580I G_kGCm'C
Cuts at: 1206 1299 2355 1206
Size: 93 1056 2432
Fragments arranged by size:
2432 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 185 793 874 1209 3353 185
Size: 608 81 335 2144 413
Fragments arranged by size:
2144 608 413 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 457
Size: 3581
Bpu10I CC'TnA_GC
Cuts at: 362 362
Size: 3581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 1636 2128 2426 757
Size: 879 492 298 1912
Fragments arranged by size:
1912 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3285 3285
Size: 3581
BsaAI yAC'GTr
Cuts at: 934 934
Size: 3581
BsaBI GATnn'nnATC
Cuts at: 6 1095 3132 6
Size: 1089 2037 455
Fragments arranged by size:
2037 1089 455
BsaHI Gr'CG_yC
Cuts at: 1012 1260 1962 3458 1012
Size: 248 702 1496 1135
Fragments arranged by size:
1496 1135 702 248
BsaWI w'CCGG_w
Cuts at: 770 1291 1969 2243 2390 2801 770
Size: 521 678 274 147 411 1550
Fragments arranged by size:
1550 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 165 397 165
Size: 232 3349
BseYI C'CCAG_C
Cuts at: 334 346 404 1006 1764 2341 2837 334
Size: 12 58 602 758 577 496 1078
Fragments arranged by size:
1078 758 602 577 496 58 12
BsiEI CG_ry'CG
Cuts at: 104 290 1123 1169 2377 104
Size: 186 833 46 1208 1308
Fragments arranged by size:
1308 1208 833 186 46
BsiHKAI G_wGCw'C
Cuts at: 359 595 1373 1563 2355 359
Size: 236 778 190 792 1585
Fragments arranged by size:
1585 792 778 236 190
BsmBI CGTCTCn'nnnn_
Cuts at: 246 246
Size: 3581
Bsp1286I G_dGCh'C
Cuts at: 190 359 595 1206 1299 1373 1563 1625 2355
Size: 169 236 611 93 74 190 62 730
Cuts at: 2355 190
Size: 1416
Fragments arranged by size:
1416 730 611 236 190 169 93 74
62
BspEI T'CCGG_A
Cuts at: 2801 2801
Size: 3581
BspHI T'CATG_A
Cuts at: 2757 2757
Size: 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 631 845 1147 1528 631
Size: 214 302 381 2684
Fragments arranged by size:
2684 381 302 214
BsrBI CCG'CTC
Cuts at: 50 314 1873 1927 3411 3576 50
Size: 264 1559 54 1484 165 55
Fragments arranged by size:
1559 1484 264 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1493 1493
Size: 3581
BsrFI r'CCGG_y
Cuts at: 1579 1760 1969 1579
Size: 181 209 3191
Fragments arranged by size:
3191 209 181
BssSI C'ACGA_G
Cuts at: 367 1852 2210 367
Size: 1485 358 1738
Fragments arranged by size:
1738 1485 358
BstAPI GCAn_nnn'nTGC
Cuts at: 372 958 372
Size: 586 2995
BstBI TT'CG_AA
Cuts at: 1942 1942
Size: 3581
BstYI r'GATC_y
Cuts at: 1 606 1073 1096 1431 1677 2689 2775 2787
Size: 605 467 23 335 246 1012 86 12
Cuts at: 2787 3133 1
Size: 346 449
Fragments arranged by size:
1012 605 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 302 302
Size: 3581
BtgI C'CryG_G
Cuts at: 149 149
Size: 3581
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1531 1712 1875 1531
Size: 181 163 3237
Fragments arranged by size:
3237 181 163
BtsI GCAGTG_nn'
Cuts at: 824 824
Size: 3581
DraI TTT'AAA
Cuts at: 580 580
Size: 3581
DrdI GACnn_nn'nnGTC
Cuts at: 1287 2145 1287
Size: 858 2723
EaeI y'GGCC_r
Cuts at: 140 221 287 1166 1340 1731 1758 3103 140
Size: 81 66 879 174 391 27 1345 618
Fragments arranged by size:
1345 879 618 391 174 81 66 27
EagI C'GGCC_G
Cuts at: 287 1166 287
Size: 879 2702
EarI CTCTTCn'nnn_
Cuts at: 1604 1814 1604
Size: 210 3371
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 322 1789 2099 2245 3391 322
Size: 1467 310 146 1146 512
Fragments arranged by size:
1467 1146 512 310 146
EcoICRI GAG'CTC
Cuts at: 357 593 357
Size: 236 3345
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 1406 1838 2585 2853 457
Size: 949 432 747 268 1185
Fragments arranged by size:
1185 949 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 421 421
Size: 3581
EcoO109I rG'GnC_Cy
Cuts at: 133 401 720 133
Size: 268 319 2994
Fragments arranged by size:
2994 319 268
EcoRI G'AATT_C
Cuts at: 585 585
Size: 3581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1600 1600
Size: 3581
FspI TGC'GCA
Cuts at: 1362 1362
Size: 3581
HaeII r_GCGC'y
Cuts at: 1015 1263 2285 3264 1015
Size: 248 1022 979 1332
Fragments arranged by size:
1332 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1423 1453 1455 1485 1423
Size: 30 2 30 3519
Fragments arranged by size:
3519 30 30 2
HincII GTy'rAC
Cuts at: 620 620
Size: 3581
KasI G'GCGC_C
Cuts at: 1011 1259 1011
Size: 248 3333
KpnI G_GTAC'C
Cuts at: 601 601
Size: 3581
MluI A'CGCG_T
Cuts at: 3014 3014
Size: 3581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2252 2436 2252
Size: 184 3397
MscI TGG'CCA
Cuts at: 223 1342 223
Size: 1119 2462
MslI CAynn'nnrTG
Cuts at: 1570 1697 1570
Size: 127 3454
MspA1I CmG'CkG
Cuts at: 322 692 1006 1366 2379 2624 322
Size: 370 314 360 1013 245 1279
Fragments arranged by size:
1279 1013 370 360 314 245
NaeI GCC'GGC
Cuts at: 1762 1762
Size: 3581
NarI GG'CG_CC
Cuts at: 1012 1260 1012
Size: 248 3333
NgoMIV G'CCGG_C
Cuts at: 1760 1760
Size: 3581
NspI r_CATG'y
Cuts at: 634 1134 2041 634
Size: 500 907 2174
Fragments arranged by size:
2174 907 500
PciI A'CATG_T
Cuts at: 2037 2037
Size: 3581
PmeI GTTT'AAAC
Cuts at: 580 580
Size: 3581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2720 2752 2720
Size: 32 3549
PpuMI rG'GwC_Cy
Cuts at: 133 401 133
Size: 268 3313
PstI C_TGCA'G
Cuts at: 628 1313 628
Size: 685 2896
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3581
PvuII CAG'CTG
Cuts at: 1006 1366 1006
Size: 360 3221
RsrII CG'GwC_CG
Cuts at: 1776 1776
Size: 3581
SacI G_AGCT'C
Cuts at: 359 595 359
Size: 236 3345
SalI G'TCGA_C
Cuts at: 618 618
Size: 3581
SanDI GG'GwC_CC
Cuts at: 401 401
Size: 3581
SapI GCTCTTCn'nnn_
Cuts at: 1604 1814 1604
Size: 210 3371
SbfI CC_TGCA'GG
Cuts at: 628 628
Size: 3581
ScaI AGT'ACT
Cuts at: 3098 3098
Size: 3581
SfcI C'TryA_G
Cuts at: 33 624 1309 2302 2493 33
Size: 591 685 993 191 1121
Fragments arranged by size:
1121 993 685 591 191
SfoI GGC'GCC
Cuts at: 1013 1261 1013
Size: 248 3333
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3581
SmaI CCC'GGG
Cuts at: 603 603
Size: 3581
SmlI C'TyrA_G
Cuts at: 736 1651 2143 2405 736
Size: 915 492 262 1912
Fragments arranged by size:
1912 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3581
SphI G_CATG'C
Cuts at: 634 1134 634
Size: 500 3081
StyI C'CwwG_G
Cuts at: 143 215 715 1069 143
Size: 72 500 354 2655
Fragments arranged by size:
2655 500 354 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1164 1297 1756 1965 1164
Size: 133 459 209 2780
Fragments arranged by size:
2780 459 209 133
TatI w'GTAC_w
Cuts at: 3096 3096
Size: 3581
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 3549
Tth111I GACn'n_nGTC
Cuts at: 1378 1378
Size: 3581
XbaI T'CTAG_A
Cuts at: 612 612
Size: 3581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1076 1076
Size: 3581
XmaI C'CCGG_G
Cuts at: 601 601
Size: 3581
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI