Restriction map for
pF1KE0148
(Circular) (Six-base) MAPSORT of: pF1KE0148.seq Check: 4510 from: 1 to: 3791
pF1KE0148 3791 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 740 740
Size: 3791
AccI GT'mk_AC
Cuts at: 829 829
Size: 3791
Acc65I G'GTAC_C
Cuts at: 807 807
Size: 3791
AclI AA'CG_TT
Cuts at: 3631 3631
Size: 3791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2048 2795 3063 1616
Size: 432 747 268 2344
Fragments arranged by size:
2344 747 432 268
AflIII A'CryG_T
Cuts at: 692 1143 2247 3224 692
Size: 451 1104 977 1259
Fragments arranged by size:
1259 1104 977 451
AgeI A'CCGG_T
Cuts at: 2179 2179
Size: 3791
AhdI GACnn_n'nnGTC
Cuts at: 150 150
Size: 3791
AleI CACnn'nnGTG
Cuts at: 421 421
Size: 3791
AlwNI CAG_nnn'CTG
Cuts at: 1096 2663 1096
Size: 1567 2224
ApaI G_GGCC'C
Cuts at: 116 757 116
Size: 641 3150
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 795 3144 3400 795
Size: 2349 256 1186
Fragments arranged by size:
2349 1186 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 461 811 461
Size: 350 3441
BamHI G'GATC_C
Cuts at: 816 816
Size: 3791
BanI G'GyrC_C
Cuts at: 807 1221 1469 1504 807
Size: 414 248 35 3094
Fragments arranged by size:
3094 414 248 35
BanII G_rGCy'C
Cuts at: 116 137 757 805 1835 116
Size: 21 620 48 1030 2072
Fragments arranged by size:
2072 1030 620 48 21
BbeI G_GCGC'C
Cuts at: 1225 1473 1225
Size: 248 3543
BbsI GAAGACnn'nnnn_
Cuts at: 503 503
Size: 3791
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 1311 1311
Size: 3791
BglII A'GATC_T
Cuts at: 1306 3343 1306
Size: 2037 1754
BlpI GC'TnA_GC
Cuts at: 903 903
Size: 3791
Bme1580I G_kGCm'C
Cuts at: 116 757 1416 1509 2565 116
Size: 641 659 93 1056 1342
Fragments arranged by size:
1342 1056 659 641 93
BmgBI CAC'GTC
Cuts at: 147 147
Size: 3791
BmrI ACTGGGnnnn_n'
Cuts at: 1003 1084 1419 3563 1003
Size: 81 335 2144 1231
Fragments arranged by size:
2144 1231 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 3791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 322 469 749 257
Size: 65 147 280 3299
Fragments arranged by size:
3299 280 147 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 967 1846 2338 2636 595
Size: 372 879 492 298 1750
Fragments arranged by size:
1750 879 492 372 298
BsaI GGTCTCn'nnnn_
Cuts at: 610 3495 610
Size: 2885 906
BsaAI yAC'GTr
Cuts at: 1144 1144
Size: 3791
BsaBI GATnn'nnATC
Cuts at: 6 346 1305 3342 6
Size: 340 959 2037 455
Fragments arranged by size:
2037 959 455 340
BsaHI Gr'CG_yC
Cuts at: 1222 1470 2172 3668 1222
Size: 248 702 1496 1345
Fragments arranged by size:
1496 1345 702 248
BsaWI w'CCGG_w
Cuts at: 710 980 1501 2179 2453 2600 3011 710
Size: 270 521 678 274 147 411 1490
Fragments arranged by size:
1490 678 521 411 274 270 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 320 350 442 472 320
Size: 30 92 30 3639
Fragments arranged by size:
3639 92 30 30
BseYI C'CCAG_C
Cuts at: 168 229 735 1216 1974 2551 3047 168
Size: 61 506 481 758 577 496 912
Fragments arranged by size:
912 758 577 506 496 481 61
BsiEI CG_ry'CG
Cuts at: 104 1333 1379 2587 104
Size: 1229 46 1208 1308
Fragments arranged by size:
1308 1229 1208 46
BsiHKAI G_wGCw'C
Cuts at: 137 805 1583 1773 2565 137
Size: 668 778 190 792 1363
Fragments arranged by size:
1363 792 778 668 190
BsmI GAATG_Cn'
Cuts at: 780 780
Size: 3791
Bsp1286I G_dGCh'C
Cuts at: 116 137 757 805 1416 1509 1583 1773 1835
Size: 21 620 48 611 93 74 190 62
Cuts at: 1835 2565 116
Size: 730 1342
Fragments arranged by size:
1342 730 620 611 190 93 74 62
48 21
BspEI T'CCGG_A
Cuts at: 3011 3011
Size: 3791
BspHI T'CATG_A
Cuts at: 248 2967 248
Size: 2719 1072
BspMI ACCTGCnnnn'nnnn_
Cuts at: 224 740 841 1055 1357 1738 224
Size: 516 101 214 302 381 2277
Fragments arranged by size:
2277 516 381 302 214 101
BsrBI CCG'CTC
Cuts at: 50 2083 2137 3621 3786 50
Size: 2033 54 1484 165 55
Fragments arranged by size:
2033 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1703 1703
Size: 3791
BsrFI r'CCGG_y
Cuts at: 188 1789 1970 2179 188
Size: 1601 181 209 1800
Fragments arranged by size:
1800 1601 209 181
BssSI C'ACGA_G
Cuts at: 2062 2420 2062
Size: 358 3433
BstAPI GCAn_nnn'nTGC
Cuts at: 1168 1168
Size: 3791
BstBI TT'CG_AA
Cuts at: 2152 2152
Size: 3791
BstXI CCAn_nnnn'nTGG
Cuts at: 666 679 666
Size: 13 3778
BstYI r'GATC_y
Cuts at: 1 179 816 1283 1306 1641 1887 2899 2985
Size: 178 637 467 23 335 246 1012 86
Cuts at: 2985 2997 3343 1
Size: 12 346 449
Fragments arranged by size:
1012 637 467 449 346 335 246 178
86 23 12
BtgI C'CryG_G
Cuts at: 107 128 665 107
Size: 21 537 3233
Fragments arranged by size:
3233 537 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1741 1922 2085 1741
Size: 181 163 3447
Fragments arranged by size:
3447 181 163
BtsI GCAGTG_nn'
Cuts at: 1034 1034
Size: 3791
DraI TTT'AAA
Cuts at: 790 790
Size: 3791
DrdI GACnn_nn'nnGTC
Cuts at: 1497 2355 1497
Size: 858 2933
EaeI y'GGCC_r
Cuts at: 681 1376 1550 1941 1968 3313 681
Size: 695 174 391 27 1345 1159
Fragments arranged by size:
1345 1159 695 391 174 27
EagI C'GGCC_G
Cuts at: 1376 1376
Size: 3791
EarI CTCTTCn'nnn_
Cuts at: 142 1814 2024 142
Size: 1672 210 1909
Fragments arranged by size:
1909 1672 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1999 2309 2455 3601 1999
Size: 310 146 1146 2189
Fragments arranged by size:
2189 1146 310 146
EcoICRI GAG'CTC
Cuts at: 135 803 135
Size: 668 3123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 322 469 749 1616 2048 2795 3063 257
Size: 65 147 280 867 432 747 268 985
Fragments arranged by size:
985 867 747 432 280 268 147 65
EcoO109I rG'GnC_Cy
Cuts at: 112 113 754 930 112
Size: 1 641 176 2973
Fragments arranged by size:
2973 641 176 1
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 384 1810 384
Size: 1426 2365
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 592 1225 1473 2495 3474 592
Size: 633 248 1022 979 909
Fragments arranged by size:
1022 979 909 633 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 318 320 350 352 355 387 442
Size: 32 150 2 30 2 3 32 55
Cuts at: 442 474 1633 1663 1665 1695 136
Size: 32 1159 30 2 30 2232
Fragments arranged by size:
2232 1159 150 55 32 32 32 30
30 30 3 2 2 2
HincII GTy'rAC
Cuts at: 830 830
Size: 3791
KasI G'GCGC_C
Cuts at: 1221 1469 1221
Size: 248 3543
KpnI G_GTAC'C
Cuts at: 811 811
Size: 3791
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2462 2646 2462
Size: 184 3607
MscI TGG'CCA
Cuts at: 683 1552 683
Size: 869 2922
MslI CAynn'nnrTG
Cuts at: 421 664 677 1780 1907 421
Size: 243 13 1103 127 2305
Fragments arranged by size:
2305 1103 243 127 13
MspA1I CmG'CkG
Cuts at: 902 1216 1576 2589 2834 902
Size: 314 360 1013 245 1859
Fragments arranged by size:
1859 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1972 1972
Size: 3791
NarI GG'CG_CC
Cuts at: 1222 1470 1222
Size: 248 3543
NcoI C'CATG_G
Cuts at: 107 107
Size: 3791
NgoMIV G'CCGG_C
Cuts at: 1970 1970
Size: 3791
NspI r_CATG'y
Cuts at: 696 844 1344 2251 696
Size: 148 500 907 2236
Fragments arranged by size:
2236 907 500 148
PasI CC'CwG_GG
Cuts at: 564 564
Size: 3791
PciI A'CATG_T
Cuts at: 692 2247 692
Size: 1555 2236
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3759
PspOMI G'GGCC_C
Cuts at: 112 753 112
Size: 641 3150
PstI C_TGCA'G
Cuts at: 221 534 555 637 838 1523 221
Size: 313 21 82 201 685 2489
Fragments arranged by size:
2489 685 313 201 82 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 1216 1576 1216
Size: 360 3431
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 137 805 137
Size: 668 3123
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SapI GCTCTTCn'nnn_
Cuts at: 142 1814 2024 142
Size: 1672 210 1909
Fragments arranged by size:
1909 1672 210
SbfI CC_TGCA'GG
Cuts at: 838 838
Size: 3791
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SfcI C'TryA_G
Cuts at: 33 217 530 551 633 834 1519 2512 2703
Size: 184 313 21 82 201 685 993 191
Cuts at: 2703 33
Size: 1121
Fragments arranged by size:
1121 993 685 313 201 191 184 82
21
SfoI GGC'GCC
Cuts at: 1223 1471 1223
Size: 248 3543
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SmaI CCC'GGG
Cuts at: 463 813 463
Size: 350 3441
SmlI C'TyrA_G
Cuts at: 610 946 1861 2353 2615 610
Size: 336 915 492 262 1786
Fragments arranged by size:
1786 915 492 336 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 844 1344 844
Size: 500 3291
StyI C'CwwG_G
Cuts at: 107 359 925 1279 107
Size: 252 566 354 2619
Fragments arranged by size:
2619 566 354 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1374 1507 1966 2175 1374
Size: 133 459 209 2990
Fragments arranged by size:
2990 459 209 133
TatI w'GTAC_w
Cuts at: 3306 3306
Size: 3791
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 3759
Tth111I GACn'n_nGTC
Cuts at: 1588 1588
Size: 3791
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 697 1286 697
Size: 589 3202
XmaI C'CCGG_G
Cuts at: 461 811 461
Size: 350 3441
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI Bpu10I BseRI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI