Restriction map for
pF1KE0144
(Circular) (Six-base) MAPSORT of: pF1KE0144.seq Check: 589 from: 1 to: 3614
pF1KE0144 3614 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 652 652
Size: 3614
Acc65I G'GTAC_C
Cuts at: 630 630
Size: 3614
AclI AA'CG_TT
Cuts at: 3454 3454
Size: 3614
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 1439 1871 2618 2886 554
Size: 885 432 747 268 1282
Fragments arranged by size:
1282 885 747 432 268
AflIII A'CryG_T
Cuts at: 966 2070 3047 966
Size: 1104 977 1533
Fragments arranged by size:
1533 1104 977
AgeI A'CCGG_T
Cuts at: 2002 2002
Size: 3614
AlwNI CAG_nnn'CTG
Cuts at: 919 2486 919
Size: 1567 2047
ApaLI G'TGCA_C
Cuts at: 2384 2384
Size: 3614
ApoI r'AATT_y
Cuts at: 618 2967 3223 618
Size: 2349 256 1009
Fragments arranged by size:
2349 1009 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3614
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3614
AvaI C'yCGr_G
Cuts at: 182 634 182
Size: 452 3162
BamHI G'GATC_C
Cuts at: 639 639
Size: 3614
BanI G'GyrC_C
Cuts at: 630 1044 1292 1327 630
Size: 414 248 35 2917
Fragments arranged by size:
2917 414 248 35
BanII G_rGCy'C
Cuts at: 169 306 494 628 1658 169
Size: 137 188 134 1030 2125
Fragments arranged by size:
2125 1030 188 137 134
BbeI G_GCGC'C
Cuts at: 1048 1296 1048
Size: 248 3366
BciVI GTATCCnnnnn_n'
Cuts at: 1512 2273 3614 1512
Size: 761 1341 1512
Fragments arranged by size:
1512 1341 761
BclI T'GATC_A
Cuts at: 228 1134 228
Size: 906 2708
BglI GCCn_nnn'nGGC
Cuts at: 112 556 112
Size: 444 3170
BglII A'GATC_T
Cuts at: 1129 3166 1129
Size: 2037 1577
BlpI GC'TnA_GC
Cuts at: 726 726
Size: 3614
Bme1580I G_kGCm'C
Cuts at: 1239 1332 2388 1239
Size: 93 1056 2465
Fragments arranged by size:
2465 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 826 907 1242 3386 826
Size: 81 335 2144 1054
Fragments arranged by size:
2144 1054 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1981 1981
Size: 3614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 393 290
Size: 103 3511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 1669 2161 2459 790
Size: 879 492 298 1945
Fragments arranged by size:
1945 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3318 3318
Size: 3614
BsaAI yAC'GTr
Cuts at: 967 967
Size: 3614
BsaBI GATnn'nnATC
Cuts at: 6 1128 3165 6
Size: 1122 2037 455
Fragments arranged by size:
2037 1122 455
BsaHI Gr'CG_yC
Cuts at: 1045 1293 1995 3491 1045
Size: 248 702 1496 1168
Fragments arranged by size:
1496 1168 702 248
BsaWI w'CCGG_w
Cuts at: 803 1324 2002 2276 2423 2834 803
Size: 521 678 274 147 411 1583
Fragments arranged by size:
1583 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 118 1039 1797 2374 2870 118
Size: 921 758 577 496 862
Fragments arranged by size:
921 862 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 499
Size: 3614
BsiEI CG_ry'CG
Cuts at: 104 1156 1202 2410 104
Size: 1052 46 1208 1308
Fragments arranged by size:
1308 1208 1052 46
BsiHKAI G_wGCw'C
Cuts at: 494 628 1406 1596 2388 494
Size: 134 778 190 792 1720
Fragments arranged by size:
1720 792 778 190 134
Bsp1286I G_dGCh'C
Cuts at: 169 306 494 628 1239 1332 1406 1596 1658
Size: 137 188 134 611 93 74 190 62
Cuts at: 1658 2388 169
Size: 730 1395
Fragments arranged by size:
1395 730 611 190 188 137 134 93
74 62
BspEI T'CCGG_A
Cuts at: 2834 2834
Size: 3614
BspHI T'CATG_A
Cuts at: 2790 2790
Size: 3614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 664 878 1180 1561 664
Size: 214 302 381 2717
Fragments arranged by size:
2717 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1906 1960 3444 3609 50
Size: 1856 54 1484 165 55
Fragments arranged by size:
1856 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1526 1526
Size: 3614
BsrFI r'CCGG_y
Cuts at: 1612 1793 2002 1612
Size: 181 209 3224
Fragments arranged by size:
3224 209 181
BssSI C'ACGA_G
Cuts at: 1885 2243 1885
Size: 358 3256
BstAPI GCAn_nnn'nTGC
Cuts at: 991 991
Size: 3614
BstBI TT'CG_AA
Cuts at: 1975 1975
Size: 3614
BstYI r'GATC_y
Cuts at: 1 639 1106 1129 1464 1710 2722 2808 2820
Size: 638 467 23 335 246 1012 86 12
Cuts at: 2820 3166 1
Size: 346 449
Fragments arranged by size:
1012 638 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3614
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1564 1745 1908 1564
Size: 181 163 3270
Fragments arranged by size:
3270 181 163
BtsI GCAGTG_nn'
Cuts at: 516 857 516
Size: 341 3273
DraI TTT'AAA
Cuts at: 378 613 378
Size: 235 3379
DrdI GACnn_nn'nnGTC
Cuts at: 1320 2178 1320
Size: 858 2756
EaeI y'GGCC_r
Cuts at: 126 1199 1373 1764 1791 3136 126
Size: 1073 174 391 27 1345 604
Fragments arranged by size:
1345 1073 604 391 174 27
EagI C'GGCC_G
Cuts at: 1199 1199
Size: 3614
EarI CTCTTCn'nnn_
Cuts at: 1637 1847 1637
Size: 210 3404
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1822 2132 2278 3424 1822
Size: 310 146 1146 2012
Fragments arranged by size:
2012 1146 310 146
EcoICRI GAG'CTC
Cuts at: 492 626 492
Size: 134 3480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 393 554 1439 1871 2618 2886 290
Size: 103 161 885 432 747 268 1018
Fragments arranged by size:
1018 885 747 432 268 161 103
EcoO109I rG'GnC_Cy
Cuts at: 257 753 257
Size: 496 3118
EcoRI G'AATT_C
Cuts at: 618 618
Size: 3614
EcoRV GAT'ATC
Cuts at: 480 480
Size: 3614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1633 1633
Size: 3614
FspI TGC'GCA
Cuts at: 1395 1395
Size: 3614
HaeII r_GCGC'y
Cuts at: 1048 1296 2318 3297 1048
Size: 248 1022 979 1365
Fragments arranged by size:
1365 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 256 288 1456 1486 1488 1518 256
Size: 32 1168 30 2 30 2352
Fragments arranged by size:
2352 1168 32 30 30 2
HincII GTy'rAC
Cuts at: 653 653
Size: 3614
KasI G'GCGC_C
Cuts at: 1044 1292 1044
Size: 248 3366
KpnI G_GTAC'C
Cuts at: 634 634
Size: 3614
MfeI C'AATT_G
Cuts at: 401 401
Size: 3614
MluI A'CGCG_T
Cuts at: 3047 3047
Size: 3614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 264 2285 2469 264
Size: 2021 184 1409
Fragments arranged by size:
2021 1409 184
MscI TGG'CCA
Cuts at: 1375 1375
Size: 3614
MslI CAynn'nnrTG
Cuts at: 1603 1730 1603
Size: 127 3487
MspA1I CmG'CkG
Cuts at: 725 1039 1399 2412 2657 725
Size: 314 360 1013 245 1682
Fragments arranged by size:
1682 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1795 1795
Size: 3614
NarI GG'CG_CC
Cuts at: 1045 1293 1045
Size: 248 3366
NcoI C'CATG_G
Cuts at: 107 107
Size: 3614
NgoMIV G'CCGG_C
Cuts at: 1793 1793
Size: 3614
NspI r_CATG'y
Cuts at: 314 667 1167 2074 314
Size: 353 500 907 1854
Fragments arranged by size:
1854 907 500 353
PciI A'CATG_T
Cuts at: 2070 2070
Size: 3614
PflMI CCAn_nnn'nTGG
Cuts at: 313 428 313
Size: 115 3499
PmeI GTTT'AAAC
Cuts at: 613 613
Size: 3614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2753 2785 2753
Size: 32 3582
PpuMI rG'GwC_Cy
Cuts at: 257 257
Size: 3614
PstI C_TGCA'G
Cuts at: 661 1346 661
Size: 685 2929
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3614
PvuII CAG'CTG
Cuts at: 1039 1399 1039
Size: 360 3254
RsrII CG'GwC_CG
Cuts at: 1809 1809
Size: 3614
SacI G_AGCT'C
Cuts at: 494 628 494
Size: 134 3480
SalI G'TCGA_C
Cuts at: 651 651
Size: 3614
SanDI GG'GwC_CC
Cuts at: 257 257
Size: 3614
SapI GCTCTTCn'nnn_
Cuts at: 1637 1847 1637
Size: 210 3404
SbfI CC_TGCA'GG
Cuts at: 661 661
Size: 3614
ScaI AGT'ACT
Cuts at: 3131 3131
Size: 3614
SfcI C'TryA_G
Cuts at: 33 657 1342 2335 2526 33
Size: 624 685 993 191 1121
Fragments arranged by size:
1121 993 685 624 191
SfoI GGC'GCC
Cuts at: 1046 1294 1046
Size: 248 3366
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3614
SmaI CCC'GGG
Cuts at: 184 636 184
Size: 452 3162
SmlI C'TyrA_G
Cuts at: 769 1684 2176 2438 769
Size: 915 492 262 1945
Fragments arranged by size:
1945 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3614
SphI G_CATG'C
Cuts at: 667 1167 667
Size: 500 3114
StyI C'CwwG_G
Cuts at: 107 485 748 1102 107
Size: 378 263 354 2619
Fragments arranged by size:
2619 378 354 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 420 1197 1330 1789 1998 420
Size: 777 133 459 209 2036
Fragments arranged by size:
2036 777 459 209 133
TatI w'GTAC_w
Cuts at: 3129 3129
Size: 3614
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 431 463 3131 3163 431
Size: 32 2668 32 882
Fragments arranged by size:
2668 882 32 32
Tth111I GACn'n_nGTC
Cuts at: 1411 1411
Size: 3614
XbaI T'CTAG_A
Cuts at: 645 645
Size: 3614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1109 1109
Size: 3614
XmaI C'CCGG_G
Cuts at: 182 634 182
Size: 452 3162
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI