Restriction map for
pF1KE0138
(Circular) (Six-base) MAPSORT of: pF1KE0138.seq Check: 8021 from: 1 to: 3923
pF1KE0138 3923 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 299 299
Size: 3923
AccI GT'mk_AC
Cuts at: 961 961
Size: 3923
Acc65I G'GTAC_C
Cuts at: 939 939
Size: 3923
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1748 2180 2927 3195 1748
Size: 432 747 268 2476
Fragments arranged by size:
2476 747 432 268
AflIII A'CryG_T
Cuts at: 1275 2379 3356 1275
Size: 1104 977 1842
Fragments arranged by size:
1842 1104 977
AgeI A'CCGG_T
Cuts at: 2311 2311
Size: 3923
AlwNI CAG_nnn'CTG
Cuts at: 123 612 632 1228 2795 123
Size: 489 20 596 1567 1251
Fragments arranged by size:
1567 1251 596 489 20
ApaI G_GGCC'C
Cuts at: 893 893
Size: 3923
ApaLI G'TGCA_C
Cuts at: 2693 2693
Size: 3923
ApoI r'AATT_y
Cuts at: 927 3276 3532 927
Size: 2349 256 1318
Fragments arranged by size:
2349 1318 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 265 884 943 265
Size: 619 59 3245
Fragments arranged by size:
3245 619 59
BamHI G'GATC_C
Cuts at: 948 948
Size: 3923
BanI G'GyrC_C
Cuts at: 270 384 939 1353 1601 1636 270
Size: 114 555 414 248 35 2557
Fragments arranged by size:
2557 555 414 248 114 35
BanII G_rGCy'C
Cuts at: 893 937 1967 893
Size: 44 1030 2849
Fragments arranged by size:
2849 1030 44
BbeI G_GCGC'C
Cuts at: 274 1357 1605 274
Size: 1083 248 2592
Fragments arranged by size:
2592 1083 248
BbsI GAAGACnn'nnnn_
Cuts at: 653 653
Size: 3923
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3889
BciVI GTATCCnnnnn_n'
Cuts at: 1821 2582 3923 1821
Size: 761 1341 1821
Fragments arranged by size:
1821 1341 761
BclI T'GATC_A
Cuts at: 1443 1443
Size: 3923
BglI GCCn_nnn'nGGC
Cuts at: 223 320 223
Size: 97 3826
BglII A'GATC_T
Cuts at: 1438 3475 1438
Size: 2037 1886
BlpI GC'TnA_GC
Cuts at: 1035 1035
Size: 3923
Bme1580I G_kGCm'C
Cuts at: 389 893 1548 1641 2697 389
Size: 504 655 93 1056 1615
Fragments arranged by size:
1615 1056 655 504 93
BmgBI CAC'GTC
Cuts at: 705 705
Size: 3923
BmrI ACTGGGnnnn_n'
Cuts at: 642 1135 1216 1551 3695 642
Size: 493 81 335 2144 870
Fragments arranged by size:
2144 870 493 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 3923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 381 400 609 757 841 381
Size: 19 209 148 84 3463
Fragments arranged by size:
3463 209 148 84 19
Bpu10I CC'TnA_GC
Cuts at: 199 199
Size: 3923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 533 1099 1978 2470 2768 533
Size: 566 879 492 298 1688
Fragments arranged by size:
1688 879 566 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 223 3627 223
Size: 3404 519
BsaAI yAC'GTr
Cuts at: 1276 1276
Size: 3923
BsaBI GATnn'nnATC
Cuts at: 6 1437 3474 6
Size: 1431 2037 455
Fragments arranged by size:
2037 1431 455
BsaHI Gr'CG_yC
Cuts at: 271 1354 1602 2304 3800 271
Size: 1083 248 702 1496 394
Fragments arranged by size:
1496 1083 702 394 248
BsaWI w'CCGG_w
Cuts at: 1112 1633 2311 2585 2732 3143 1112
Size: 521 678 274 147 411 1892
Fragments arranged by size:
1892 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 657 706 709 657
Size: 49 3 3871
Fragments arranged by size:
3871 49 3
BseYI C'CCAG_C
Cuts at: 153 1348 2106 2683 3179 153
Size: 1195 758 577 496 897
Fragments arranged by size:
1195 897 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 694 244
Size: 450 3473
BsiEI CG_ry'CG
Cuts at: 104 338 1465 1511 2719 104
Size: 234 1127 46 1208 1308
Fragments arranged by size:
1308 1208 1127 234 46
BsiHKAI G_wGCw'C
Cuts at: 667 937 1715 1905 2697 667
Size: 270 778 190 792 1893
Fragments arranged by size:
1893 792 778 270 190
Bsp1286I G_dGCh'C
Cuts at: 389 667 893 937 1548 1641 1715 1905 1967
Size: 278 226 44 611 93 74 190 62
Cuts at: 1967 2697 389
Size: 730 1615
Fragments arranged by size:
1615 730 611 278 226 190 93 74
62 44
BspEI T'CCGG_A
Cuts at: 3143 3143
Size: 3923
BspHI T'CATG_A
Cuts at: 3099 3099
Size: 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 299 917 973 1187 1489 1870 299
Size: 618 56 214 302 381 2352
Fragments arranged by size:
2352 618 381 302 214 56
BsrBI CCG'CTC
Cuts at: 50 2215 2269 3753 3918 50
Size: 2165 54 1484 165 55
Fragments arranged by size:
2165 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1835 1835
Size: 3923
BsrFI r'CCGG_y
Cuts at: 310 391 412 749 1921 2102 2311 310
Size: 81 21 337 1172 181 209 1922
Fragments arranged by size:
1922 1172 337 209 181 81 21
BssHII G'CGCG_C
Cuts at: 163 165 863 163
Size: 2 698 3223
Fragments arranged by size:
3223 698 2
BssSI C'ACGA_G
Cuts at: 429 2194 2552 429
Size: 1765 358 1800
Fragments arranged by size:
1800 1765 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1300 1300
Size: 3923
BstBI TT'CG_AA
Cuts at: 2284 2284
Size: 3923
BstXI CCAn_nnnn'nTGG
Cuts at: 252 252
Size: 3923
BstYI r'GATC_y
Cuts at: 1 948 1415 1438 1773 2019 3031 3117 3129
Size: 947 467 23 335 246 1012 86 12
Cuts at: 3129 3475 1
Size: 346 449
Fragments arranged by size:
1012 947 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 478 478
Size: 3923
BtgI C'CryG_G
Cuts at: 446 446
Size: 3923
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1873 2054 2217 1873
Size: 181 163 3579
Fragments arranged by size:
3579 181 163
BtsI GCAGTG_nn'
Cuts at: 1166 1166
Size: 3923
DraI TTT'AAA
Cuts at: 922 922
Size: 3923
DraIII CAC_nnn'GTG
Cuts at: 447 447
Size: 3923
DrdI GACnn_nn'nnGTC
Cuts at: 1629 2487 1629
Size: 858 3065
EaeI y'GGCC_r
Cuts at: 224 312 335 728 1508 1682 2073 2100 3445
Size: 88 23 393 780 174 391 27 1345
Cuts at: 3445 224
Size: 702
Fragments arranged by size:
1345 780 702 393 391 174 88 27
23
EagI C'GGCC_G
Cuts at: 335 1508 335
Size: 1173 2750
EarI CTCTTCn'nnn_
Cuts at: 331 1946 2156 331
Size: 1615 210 2098
Fragments arranged by size:
2098 1615 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2131 2441 2587 3733 2131
Size: 310 146 1146 2321
Fragments arranged by size:
2321 1146 310 146
EcoICRI GAG'CTC
Cuts at: 935 935
Size: 3923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 381 400 609 757 841 1748 2180 2927 3195
Size: 19 209 148 84 907 432 747 268
Cuts at: 3195 381
Size: 1109
Fragments arranged by size:
1109 907 747 432 268 209 148 84
19
EcoNI CCTnn'n_nnAGG
Cuts at: 202 202
Size: 3923
EcoO109I rG'GnC_Cy
Cuts at: 148 194 321 785 889 1062 148
Size: 46 127 464 104 173 3009
Fragments arranged by size:
3009 464 173 127 104 46
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 804 1942 804
Size: 1138 2785
FspI TGC'GCA
Cuts at: 1704 1704
Size: 3923
HaeII r_GCGC'y
Cuts at: 160 259 274 1357 1605 2627 3606 160
Size: 99 15 1083 248 1022 979 477
Fragments arranged by size:
1083 1022 979 477 248 99 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1765 1795 1797 1827 1765
Size: 30 2 30 3861
Fragments arranged by size:
3861 30 30 2
HincII GTy'rAC
Cuts at: 962 962
Size: 3923
KasI G'GCGC_C
Cuts at: 270 1353 1601 270
Size: 1083 248 2592
Fragments arranged by size:
2592 1083 248
KpnI G_GTAC'C
Cuts at: 943 943
Size: 3923
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2778 2594
Size: 184 3739
MscI TGG'CCA
Cuts at: 730 1684 730
Size: 954 2969
MslI CAynn'nnrTG
Cuts at: 1912 2039 1912
Size: 127 3796
MspA1I CmG'CkG
Cuts at: 120 177 612 1034 1348 1708 2721 2966 120
Size: 57 435 422 314 360 1013 245 1077
Fragments arranged by size:
1077 1013 435 422 360 314 245 57
NaeI GCC'GGC
Cuts at: 312 393 2104 312
Size: 81 1711 2131
Fragments arranged by size:
2131 1711 81
NarI GG'CG_CC
Cuts at: 271 1354 1602 271
Size: 1083 248 2592
Fragments arranged by size:
2592 1083 248
NgoMIV G'CCGG_C
Cuts at: 310 391 2102 310
Size: 81 1711 2131
Fragments arranged by size:
2131 1711 81
NspI r_CATG'y
Cuts at: 378 976 1476 2383 378
Size: 598 500 907 1918
Fragments arranged by size:
1918 907 598 500
PciI A'CATG_T
Cuts at: 2379 2379
Size: 3923
PflMI CCAn_nnn'nTGG
Cuts at: 447 632 447
Size: 185 3738
PfoI T'CCnGG_A
Cuts at: 452 641 758 452
Size: 189 117 3617
Fragments arranged by size:
3617 189 117
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 3891
PpuMI rG'GwC_Cy
Cuts at: 148 785 148
Size: 637 3286
PsiI TTA'TAA
Cuts at: 497 497
Size: 3923
PspOMI G'GGCC_C
Cuts at: 889 889
Size: 3923
PstI C_TGCA'G
Cuts at: 719 970 1655 719
Size: 251 685 2987
Fragments arranged by size:
2987 685 251
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 120 177 612 1348 1708 120
Size: 57 435 736 360 2335
Fragments arranged by size:
2335 736 435 360 57
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 937 937
Size: 3923
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SanDI GG'GwC_CC
Cuts at: 148 148
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 3440 3440
Size: 3923
SfcI C'TryA_G
Cuts at: 33 715 966 1651 2644 2835 33
Size: 682 251 685 993 191 1121
Fragments arranged by size:
1121 993 685 682 251 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 320 320
Size: 3923
SfoI GGC'GCC
Cuts at: 272 1355 1603 272
Size: 1083 248 2592
Fragments arranged by size:
2592 1083 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 886 945 886
Size: 59 3864
SmlI C'TyrA_G
Cuts at: 548 1078 1993 2485 2747 548
Size: 530 915 492 262 1724
Fragments arranged by size:
1724 915 530 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 378 976 1476 378
Size: 598 500 2825
Fragments arranged by size:
2825 598 500
StuI AGG'CCT
Cuts at: 682 682
Size: 3923
StyI C'CwwG_G
Cuts at: 1057 1411 1057
Size: 354 3569
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1506 1639 2098 2307 1506
Size: 133 459 209 3122
Fragments arranged by size:
3122 459 209 133
TatI w'GTAC_w
Cuts at: 3438 3438
Size: 3923
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 3891
Tth111I GACn'n_nGTC
Cuts at: 703 1720 703
Size: 1017 2906
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1418 1418
Size: 3923
XmaI C'CCGG_G
Cuts at: 884 943 884
Size: 59 3864
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BstEII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PspXI PsrI
SacII SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI