Restriction map for
pF1KE0131
(Circular) (Six-base) MAPSORT of: pF1KE0131.seq Check: 6851 from: 1 to: 3662
pF1KE0131 3662 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 700 700
Size: 3662
Acc65I G'GTAC_C
Cuts at: 678 678
Size: 3662
AclI AA'CG_TT
Cuts at: 3502 3502
Size: 3662
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 1487 1919 2666 2934 278
Size: 1209 432 747 268 1006
Fragments arranged by size:
1209 1006 747 432 268
AflIII A'CryG_T
Cuts at: 1014 2118 3095 1014
Size: 1104 977 1581
Fragments arranged by size:
1581 1104 977
AgeI A'CCGG_T
Cuts at: 2050 2050
Size: 3662
AlwNI CAG_nnn'CTG
Cuts at: 967 2534 967
Size: 1567 2095
ApaLI G'TGCA_C
Cuts at: 2432 2432
Size: 3662
ApoI r'AATT_y
Cuts at: 666 3015 3271 666
Size: 2349 256 1057
Fragments arranged by size:
2349 1057 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3662
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
AvaI C'yCGr_G
Cuts at: 239 682 239
Size: 443 3219
BamHI G'GATC_C
Cuts at: 687 687
Size: 3662
BanI G'GyrC_C
Cuts at: 244 571 581 678 1092 1340 1375 244
Size: 327 10 97 414 248 35 2531
Fragments arranged by size:
2531 414 327 248 97 35 10
BanII G_rGCy'C
Cuts at: 676 1706 676
Size: 1030 2632
BbeI G_GCGC'C
Cuts at: 575 585 1096 1344 575
Size: 10 511 248 2893
Fragments arranged by size:
2893 511 248 10
BbsI GAAGACnn'nnnn_
Cuts at: 528 528
Size: 3662
BciVI GTATCCnnnnn_n'
Cuts at: 1560 2321 3662 1560
Size: 761 1341 1560
Fragments arranged by size:
1560 1341 761
BclI T'GATC_A
Cuts at: 599 1182 599
Size: 583 3079
BglII A'GATC_T
Cuts at: 1177 3214 1177
Size: 2037 1625
BlpI GC'TnA_GC
Cuts at: 774 774
Size: 3662
Bme1580I G_kGCm'C
Cuts at: 1287 1380 2436 1287
Size: 93 1056 2513
Fragments arranged by size:
2513 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 874 955 1290 3434 874
Size: 81 335 2144 1102
Fragments arranged by size:
2144 1102 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 3662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 523 580 385
Size: 138 57 3467
Fragments arranged by size:
3467 138 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1717 2209 2507 838
Size: 879 492 298 1993
Fragments arranged by size:
1993 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 586 3366 586
Size: 2780 882
BsaAI yAC'GTr
Cuts at: 1015 1015
Size: 3662
BsaBI GATnn'nnATC
Cuts at: 6 395 1176 3213 6
Size: 389 781 2037 455
Fragments arranged by size:
2037 781 455 389
BsaHI Gr'CG_yC
Cuts at: 177 546 572 582 1093 1341 2043 3539 177
Size: 369 26 10 511 248 702 1496 300
Fragments arranged by size:
1496 702 511 369 300 248 26 10
BsaWI w'CCGG_w
Cuts at: 851 1372 2050 2324 2471 2882 851
Size: 521 678 274 147 411 1631
Fragments arranged by size:
1631 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 347 1087 1845 2422 2918 347
Size: 740 758 577 496 1091
Fragments arranged by size:
1091 758 740 577 496
BsiEI CG_ry'CG
Cuts at: 104 1204 1250 2458 104
Size: 1100 46 1208 1308
Fragments arranged by size:
1308 1208 1100 46
BsiHKAI G_wGCw'C
Cuts at: 200 676 1454 1644 2436 200
Size: 476 778 190 792 1426
Fragments arranged by size:
1426 792 778 476 190
BsmI GAATG_Cn'
Cuts at: 371 371
Size: 3662
Bsp1286I G_dGCh'C
Cuts at: 200 676 1287 1380 1454 1644 1706 2436 200
Size: 476 611 93 74 190 62 730 1426
Fragments arranged by size:
1426 730 611 476 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2882 2882
Size: 3662
BspHI T'CATG_A
Cuts at: 515 2838 515
Size: 2323 1339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 712 926 1228 1609 712
Size: 214 302 381 2765
Fragments arranged by size:
2765 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1954 2008 3492 3657 50
Size: 1904 54 1484 165 55
Fragments arranged by size:
1904 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 626 1574 626
Size: 948 2714
BsrFI r'CCGG_y
Cuts at: 542 1660 1841 2050 542
Size: 1118 181 209 2154
Fragments arranged by size:
2154 1118 209 181
BssHII G'CGCG_C
Cuts at: 552 552
Size: 3662
BssSI C'ACGA_G
Cuts at: 1933 2291 1933
Size: 358 3304
BstAPI GCAn_nnn'nTGC
Cuts at: 1039 1039
Size: 3662
BstBI TT'CG_AA
Cuts at: 2023 2023
Size: 3662
BstXI CCAn_nnnn'nTGG
Cuts at: 339 339
Size: 3662
BstYI r'GATC_y
Cuts at: 1 229 687 1154 1177 1512 1758 2770 2856
Size: 228 458 467 23 335 246 1012 86
Cuts at: 2856 2868 3214 1
Size: 12 346 449
Fragments arranged by size:
1012 467 458 449 346 335 246 228
86 23 12
BtgI C'CryG_G
Cuts at: 107 384 107
Size: 277 3385
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 588 1612 1793 1956 588
Size: 1024 181 163 2294
Fragments arranged by size:
2294 1024 181 163
BtsI GCAGTG_nn'
Cuts at: 473 905 473
Size: 432 3230
DraI TTT'AAA
Cuts at: 661 661
Size: 3662
DraIII CAC_nnn'GTG
Cuts at: 204 204
Size: 3662
DrdI GACnn_nn'nnGTC
Cuts at: 1368 2226 1368
Size: 858 2804
EaeI y'GGCC_r
Cuts at: 620 1247 1421 1812 1839 3184 620
Size: 627 174 391 27 1345 1098
Fragments arranged by size:
1345 1098 627 391 174 27
EagI C'GGCC_G
Cuts at: 1247 1247
Size: 3662
EarI CTCTTCn'nnn_
Cuts at: 482 1685 1895 482
Size: 1203 210 2249
Fragments arranged by size:
2249 1203 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 151 1870 2180 2326 3472 151
Size: 1719 310 146 1146 341
Fragments arranged by size:
1719 1146 341 310 146
EcoICRI GAG'CTC
Cuts at: 674 674
Size: 3662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 385 523 580 1487 1919 2666 2934 278
Size: 107 138 57 907 432 747 268 1006
Fragments arranged by size:
1006 907 747 432 268 138 107 57
EcoO109I rG'GnC_Cy
Cuts at: 118 344 801 118
Size: 226 457 2979
Fragments arranged by size:
2979 457 226
EcoRI G'AATT_C
Cuts at: 666 666
Size: 3662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1681 1681
Size: 3662
FspI TGC'GCA
Cuts at: 1443 1443
Size: 3662
HaeII r_GCGC'y
Cuts at: 575 585 1096 1344 2366 3345 575
Size: 10 511 248 1022 979 892
Fragments arranged by size:
1022 979 892 511 248 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 214 246 1504 1534 1536 1566 214
Size: 32 1258 30 2 30 2310
Fragments arranged by size:
2310 1258 32 30 30 2
HincII GTy'rAC
Cuts at: 540 701 540
Size: 161 3501
KasI G'GCGC_C
Cuts at: 571 581 1092 1340 571
Size: 10 511 248 2893
Fragments arranged by size:
2893 511 248 10
KpnI G_GTAC'C
Cuts at: 682 682
Size: 3662
MluI A'CGCG_T
Cuts at: 3095 3095
Size: 3662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 2333 2517 248
Size: 2085 184 1393
Fragments arranged by size:
2085 1393 184
MscI TGG'CCA
Cuts at: 1423 1423
Size: 3662
MslI CAynn'nnrTG
Cuts at: 389 607 1651 1778 389
Size: 218 1044 127 2273
Fragments arranged by size:
2273 1044 218 127
MspA1I CmG'CkG
Cuts at: 351 528 773 1087 1447 2460 2705 351
Size: 177 245 314 360 1013 245 1308
Fragments arranged by size:
1308 1013 360 314 245 245 177
NaeI GCC'GGC
Cuts at: 1843 1843
Size: 3662
NarI GG'CG_CC
Cuts at: 572 582 1093 1341 572
Size: 10 511 248 2893
Fragments arranged by size:
2893 511 248 10
NcoI C'CATG_G
Cuts at: 107 384 107
Size: 277 3385
NgoMIV G'CCGG_C
Cuts at: 1841 1841
Size: 3662
NspI r_CATG'y
Cuts at: 715 1215 2122 715
Size: 500 907 2255
Fragments arranged by size:
2255 907 500
PasI CC'CwG_GG
Cuts at: 454 454
Size: 3662
PciI A'CATG_T
Cuts at: 2118 2118
Size: 3662
PflMI CCAn_nnn'nTGG
Cuts at: 638 638
Size: 3662
PfoI T'CCnGG_A
Cuts at: 401 401
Size: 3662
PmeI GTTT'AAAC
Cuts at: 661 661
Size: 3662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2801 2833 2801
Size: 32 3630
PpuMI rG'GwC_Cy
Cuts at: 344 344
Size: 3662
PstI C_TGCA'G
Cuts at: 709 1394 709
Size: 685 2977
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3662
PvuII CAG'CTG
Cuts at: 351 528 1087 1447 351
Size: 177 559 360 2566
Fragments arranged by size:
2566 559 360 177
RsrII CG'GwC_CG
Cuts at: 1857 1857
Size: 3662
SacI G_AGCT'C
Cuts at: 676 676
Size: 3662
SalI G'TCGA_C
Cuts at: 699 699
Size: 3662
SanDI GG'GwC_CC
Cuts at: 344 344
Size: 3662
SapI GCTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
SbfI CC_TGCA'GG
Cuts at: 709 709
Size: 3662
ScaI AGT'ACT
Cuts at: 3179 3179
Size: 3662
SexAI A'CCwGG_T
Cuts at: 200 653 200
Size: 453 3209
SfcI C'TryA_G
Cuts at: 33 705 1390 2383 2574 33
Size: 672 685 993 191 1121
Fragments arranged by size:
1121 993 685 672 191
SfoI GGC'GCC
Cuts at: 573 583 1094 1342 573
Size: 10 511 248 2893
Fragments arranged by size:
2893 511 248 10
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
SmaI CCC'GGG
Cuts at: 241 684 241
Size: 443 3219
SmlI C'TyrA_G
Cuts at: 817 1732 2224 2486 817
Size: 915 492 262 1993
Fragments arranged by size:
1993 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3662
SphI G_CATG'C
Cuts at: 715 1215 715
Size: 500 3162
StyI C'CwwG_G
Cuts at: 107 131 180 384 423 796 1150 107
Size: 24 49 204 39 373 354 2619
Fragments arranged by size:
2619 373 354 204 49 39 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 224 1245 1378 1837 2046 224
Size: 1021 133 459 209 1840
Fragments arranged by size:
1840 1021 459 209 133
TatI w'GTAC_w
Cuts at: 498 3177 498
Size: 2679 983
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 3630
Tth111I GACn'n_nGTC
Cuts at: 1459 1459
Size: 3662
XbaI T'CTAG_A
Cuts at: 693 693
Size: 3662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 138 409 1157 138
Size: 271 748 2643
Fragments arranged by size:
2643 748 271
XmaI C'CCGG_G
Cuts at: 239 682 239
Size: 443 3219
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI