Restriction map for
pF1KE0128
(Circular) (Six-base) MAPSORT of: pF1KE0128.seq Check: 7949 from: 1 to: 3416
pF1KE0128 3416 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 454 454
Size: 3416
Acc65I G'GTAC_C
Cuts at: 432 432
Size: 3416
AclI AA'CG_TT
Cuts at: 3256 3256
Size: 3416
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 1241 1673 2420 2688 208
Size: 1033 432 747 268 936
Fragments arranged by size:
1033 936 747 432 268
AflIII A'CryG_T
Cuts at: 768 1872 2849 768
Size: 1104 977 1335
Fragments arranged by size:
1335 1104 977
AgeI A'CCGG_T
Cuts at: 1804 1804
Size: 3416
AleI CACnn'nnGTG
Cuts at: 387 387
Size: 3416
AlwNI CAG_nnn'CTG
Cuts at: 721 2288 721
Size: 1567 1849
ApaLI G'TGCA_C
Cuts at: 2186 2186
Size: 3416
ApoI r'AATT_y
Cuts at: 420 2769 3025 420
Size: 2349 256 811
Fragments arranged by size:
2349 811 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3416
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3416
AvaI C'yCGr_G
Cuts at: 436 436
Size: 3416
BamHI G'GATC_C
Cuts at: 441 441
Size: 3416
BanI G'GyrC_C
Cuts at: 271 432 846 1094 1129 271
Size: 161 414 248 35 2558
Fragments arranged by size:
2558 414 248 161 35
BanII G_rGCy'C
Cuts at: 430 1460 430
Size: 1030 2386
BbeI G_GCGC'C
Cuts at: 275 850 1098 275
Size: 575 248 2593
Fragments arranged by size:
2593 575 248
BbsI GAAGACnn'nnnn_
Cuts at: 196 196
Size: 3416
BciVI GTATCCnnnnn_n'
Cuts at: 1314 2075 3416 1314
Size: 761 1341 1314
Fragments arranged by size:
1341 1314 761
BclI T'GATC_A
Cuts at: 218 936 218
Size: 718 2698
BglII A'GATC_T
Cuts at: 931 2968 931
Size: 2037 1379
BlpI GC'TnA_GC
Cuts at: 528 528
Size: 3416
Bme1580I G_kGCm'C
Cuts at: 1041 1134 2190 1041
Size: 93 1056 2267
Fragments arranged by size:
2267 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 628 709 1044 3188 628
Size: 81 335 2144 856
Fragments arranged by size:
2144 856 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 1471 1963 2261 592
Size: 879 492 298 1747
Fragments arranged by size:
1747 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3120 3120
Size: 3416
BsaAI yAC'GTr
Cuts at: 769 769
Size: 3416
BsaBI GATnn'nnATC
Cuts at: 6 930 2967 6
Size: 924 2037 455
Fragments arranged by size:
2037 924 455
BsaHI Gr'CG_yC
Cuts at: 272 847 1095 1797 3293 272
Size: 575 248 702 1496 395
Fragments arranged by size:
1496 702 575 395 248
BsaWI w'CCGG_w
Cuts at: 605 1126 1804 2078 2225 2636 605
Size: 521 678 274 147 411 1385
Fragments arranged by size:
1385 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 841 1599 2176 2672 841
Size: 758 577 496 1585
Fragments arranged by size:
1585 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 958 1004 2212 104
Size: 854 46 1208 1308
Fragments arranged by size:
1308 1208 854 46
BsiHKAI G_wGCw'C
Cuts at: 430 1208 1398 2190 430
Size: 778 190 792 1656
Fragments arranged by size:
1656 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 430 1041 1134 1208 1398 1460 2190 430
Size: 611 93 74 190 62 730 1656
Fragments arranged by size:
1656 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2636 2636
Size: 3416
BspHI T'CATG_A
Cuts at: 2592 2592
Size: 3416
BspMI ACCTGCnnnn'nnnn_
Cuts at: 466 680 982 1363 466
Size: 214 302 381 2519
Fragments arranged by size:
2519 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1708 1762 3246 3411 50
Size: 1658 54 1484 165 55
Fragments arranged by size:
1658 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1328 1328
Size: 3416
BsrFI r'CCGG_y
Cuts at: 1414 1595 1804 1414
Size: 181 209 3026
Fragments arranged by size:
3026 209 181
BssSI C'ACGA_G
Cuts at: 1687 2045 1687
Size: 358 3058
BstAPI GCAn_nnn'nTGC
Cuts at: 793 793
Size: 3416
BstBI TT'CG_AA
Cuts at: 1777 1777
Size: 3416
BstYI r'GATC_y
Cuts at: 1 441 908 931 1266 1512 2524 2610 2622
Size: 440 467 23 335 246 1012 86 12
Cuts at: 2622 2968 1
Size: 346 449
Fragments arranged by size:
1012 467 449 440 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3416
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1366 1547 1710 1366
Size: 181 163 3072
Fragments arranged by size:
3072 181 163
BtsI GCAGTG_nn'
Cuts at: 139 329 659 139
Size: 190 330 2896
Fragments arranged by size:
2896 330 190
DraI TTT'AAA
Cuts at: 415 415
Size: 3416
DrdI GACnn_nn'nnGTC
Cuts at: 1122 1980 1122
Size: 858 2558
EaeI y'GGCC_r
Cuts at: 1001 1175 1566 1593 2938 1001
Size: 174 391 27 1345 1479
Fragments arranged by size:
1479 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1001 1001
Size: 3416
EarI CTCTTCn'nnn_
Cuts at: 1439 1649 1439
Size: 210 3206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1624 1934 2080 3226 1624
Size: 310 146 1146 1814
Fragments arranged by size:
1814 1146 310 146
EcoICRI GAG'CTC
Cuts at: 428 428
Size: 3416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 1241 1673 2420 2688 208
Size: 1033 432 747 268 936
Fragments arranged by size:
1033 936 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 555 555
Size: 3416
EcoRI G'AATT_C
Cuts at: 420 420
Size: 3416
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 292 1435 292
Size: 1143 2273
FspI TGC'GCA
Cuts at: 1197 1197
Size: 3416
HaeII r_GCGC'y
Cuts at: 117 133 275 850 1098 2120 3099 117
Size: 16 142 575 248 1022 979 434
Fragments arranged by size:
1022 979 575 434 248 142 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1258 1288 1290 1320 1258
Size: 30 2 30 3354
Fragments arranged by size:
3354 30 30 2
HincII GTy'rAC
Cuts at: 455 455
Size: 3416
KasI G'GCGC_C
Cuts at: 271 846 1094 271
Size: 575 248 2593
Fragments arranged by size:
2593 575 248
KpnI G_GTAC'C
Cuts at: 436 436
Size: 3416
MluI A'CGCG_T
Cuts at: 2849 2849
Size: 3416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2087 2271 2087
Size: 184 3232
MscI TGG'CCA
Cuts at: 1177 1177
Size: 3416
MslI CAynn'nnrTG
Cuts at: 387 1405 1532 387
Size: 1018 127 2271
Fragments arranged by size:
2271 1018 127
MspA1I CmG'CkG
Cuts at: 527 841 1201 2214 2459 527
Size: 314 360 1013 245 1484
Fragments arranged by size:
1484 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1597 1597
Size: 3416
NarI GG'CG_CC
Cuts at: 272 847 1095 272
Size: 575 248 2593
Fragments arranged by size:
2593 575 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3416
NgoMIV G'CCGG_C
Cuts at: 1595 1595
Size: 3416
NspI r_CATG'y
Cuts at: 469 969 1876 469
Size: 500 907 2009
Fragments arranged by size:
2009 907 500
PciI A'CATG_T
Cuts at: 1872 1872
Size: 3416
PmeI GTTT'AAAC
Cuts at: 415 415
Size: 3416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2555 2587 2555
Size: 32 3384
PstI C_TGCA'G
Cuts at: 146 463 1148 146
Size: 317 685 2414
Fragments arranged by size:
2414 685 317
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3416
PvuII CAG'CTG
Cuts at: 841 1201 841
Size: 360 3056
RsrII CG'GwC_CG
Cuts at: 1611 1611
Size: 3416
SacI G_AGCT'C
Cuts at: 430 430
Size: 3416
SalI G'TCGA_C
Cuts at: 453 453
Size: 3416
SapI GCTCTTCn'nnn_
Cuts at: 1439 1649 1439
Size: 210 3206
SbfI CC_TGCA'GG
Cuts at: 463 463
Size: 3416
ScaI AGT'ACT
Cuts at: 2933 2933
Size: 3416
SfcI C'TryA_G
Cuts at: 33 142 459 1144 2137 2328 33
Size: 109 317 685 993 191 1121
Fragments arranged by size:
1121 993 685 317 191 109
SfoI GGC'GCC
Cuts at: 273 848 1096 273
Size: 575 248 2593
Fragments arranged by size:
2593 575 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3416
SmaI CCC'GGG
Cuts at: 438 438
Size: 3416
SmlI C'TyrA_G
Cuts at: 571 1486 1978 2240 571
Size: 915 492 262 1747
Fragments arranged by size:
1747 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3416
SphI G_CATG'C
Cuts at: 469 969 469
Size: 500 2916
StyI C'CwwG_G
Cuts at: 107 276 550 904 107
Size: 169 274 354 2619
Fragments arranged by size:
2619 354 274 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 999 1132 1591 1800 999
Size: 133 459 209 2615
Fragments arranged by size:
2615 459 209 133
TatI w'GTAC_w
Cuts at: 2931 2931
Size: 3416
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2933 2965 2933
Size: 32 3384
Tth111I GACn'n_nGTC
Cuts at: 1213 1213
Size: 3416
XbaI T'CTAG_A
Cuts at: 447 447
Size: 3416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 911 911
Size: 3416
XmaI C'CCGG_G
Cuts at: 436 436
Size: 3416
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI