Restriction map for
pF1KE0122
(Circular) (Six-base) MAPSORT of: pF1KE0122.seq Check: 2141 from: 1 to: 3908
pF1KE0122 3908 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 946 946
Size: 3908
Acc65I G'GTAC_C
Cuts at: 581 924 581
Size: 343 3565
AclI AA'CG_TT
Cuts at: 3748 3748
Size: 3908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 1733 2165 2912 3180 373
Size: 1360 432 747 268 1101
Fragments arranged by size:
1360 1101 747 432 268
AfeI AGC'GCT
Cuts at: 257 257
Size: 3908
AflIII A'CryG_T
Cuts at: 1260 2364 3341 1260
Size: 1104 977 1827
Fragments arranged by size:
1827 1104 977
AgeI A'CCGG_T
Cuts at: 2296 2296
Size: 3908
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 499 531 499
Size: 32 3876
AlwNI CAG_nnn'CTG
Cuts at: 402 459 738 840 1213 2780 402
Size: 57 279 102 373 1567 1530
Fragments arranged by size:
1567 1530 373 279 102 57
ApaLI G'TGCA_C
Cuts at: 592 2678 592
Size: 2086 1822
ApoI r'AATT_y
Cuts at: 912 3261 3517 912
Size: 2349 256 1303
Fragments arranged by size:
2349 1303 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3908
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
AvaI C'yCGr_G
Cuts at: 153 527 928 153
Size: 374 401 3133
Fragments arranged by size:
3133 401 374
BamHI G'GATC_C
Cuts at: 381 933 381
Size: 552 3356
BanI G'GyrC_C
Cuts at: 205 391 581 924 1338 1586 1621 205
Size: 186 190 343 414 248 35 2492
Fragments arranged by size:
2492 414 343 248 190 186 35
BanII G_rGCy'C
Cuts at: 152 795 849 922 1952 152
Size: 643 54 73 1030 2108
Fragments arranged by size:
2108 1030 643 73 54
BbeI G_GCGC'C
Cuts at: 209 1342 1590 209
Size: 1133 248 2527
Fragments arranged by size:
2527 1133 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 102 136 102
Size: 34 3874
BciVI GTATCCnnnnn_n'
Cuts at: 1806 2567 3908 1806
Size: 761 1341 1806
Fragments arranged by size:
1806 1341 761
BclI T'GATC_A
Cuts at: 1428 1428
Size: 3908
BglII A'GATC_T
Cuts at: 1423 3460 1423
Size: 2037 1871
BlpI GC'TnA_GC
Cuts at: 1020 1020
Size: 3908
Bme1580I G_kGCm'C
Cuts at: 396 596 1533 1626 2682 396
Size: 200 937 93 1056 1622
Fragments arranged by size:
1622 1056 937 200 93
BmgBI CAC'GTC
Cuts at: 888 888
Size: 3908
BmrI ACTGGGnnnn_n'
Cuts at: 1120 1201 1536 3680 1120
Size: 81 335 2144 1348
Fragments arranged by size:
2144 1348 335 81
BmtI G_CTAG'C
Cuts at: 565 565
Size: 3908
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2275 2275
Size: 3908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 436
Size: 3908
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1084 1963 2455 2753 1084
Size: 879 492 298 2239
Fragments arranged by size:
2239 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 325 519 756 3612 325
Size: 194 237 2856 621
Fragments arranged by size:
2856 621 237 194
BsaAI yAC'GTr
Cuts at: 1261 1261
Size: 3908
BsaBI GATnn'nnATC
Cuts at: 6 1422 3459 6
Size: 1416 2037 455
Fragments arranged by size:
2037 1416 455
BsaHI Gr'CG_yC
Cuts at: 206 1339 1587 2289 3785 206
Size: 1133 248 702 1496 329
Fragments arranged by size:
1496 1133 702 329 248
BsaWI w'CCGG_w
Cuts at: 1097 1618 2296 2570 2717 3128 1097
Size: 521 678 274 147 411 1877
Fragments arranged by size:
1877 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 499 529 550 580 499
Size: 30 21 30 3827
Fragments arranged by size:
3827 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 390 571 871 390
Size: 181 300 3427
Fragments arranged by size:
3427 300 181
BseYI C'CCAG_C
Cuts at: 615 827 861 1333 2091 2668 3164 615
Size: 212 34 472 758 577 496 1359
Fragments arranged by size:
1359 758 577 496 472 212 34
BsiEI CG_ry'CG
Cuts at: 104 480 1450 1496 2704 104
Size: 376 970 46 1208 1308
Fragments arranged by size:
1308 1208 970 376 46
BsiHKAI G_wGCw'C
Cuts at: 534 596 922 1700 1890 2682 534
Size: 62 326 778 190 792 1760
Fragments arranged by size:
1760 792 778 326 190 62
BsmI GAATG_Cn'
Cuts at: 703 703
Size: 3908
Bsp1286I G_dGCh'C
Cuts at: 152 396 534 596 795 849 922 1533 1626
Size: 244 138 62 199 54 73 611 93
Cuts at: 1626 1700 1890 1952 2682 152
Size: 74 190 62 730 1378
Fragments arranged by size:
1378 730 611 244 199 190 138 93
74 73 62 62 54
BspEI T'CCGG_A
Cuts at: 3128 3128
Size: 3908
BspHI T'CATG_A
Cuts at: 3084 3084
Size: 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 958 1172 1474 1855 958
Size: 214 302 381 3011
Fragments arranged by size:
3011 381 302 214
BsrBI CCG'CTC
Cuts at: 50 822 2200 2254 3738 3903 50
Size: 772 1378 54 1484 165 55
Fragments arranged by size:
1484 1378 772 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1820 1820
Size: 3908
BsrFI r'CCGG_y
Cuts at: 208 268 1906 2087 2296 208
Size: 60 1638 181 209 1820
Fragments arranged by size:
1820 1638 209 181 60
BssSI C'ACGA_G
Cuts at: 2179 2537 2179
Size: 358 3550
BstAPI GCAn_nnn'nTGC
Cuts at: 600 1285 600
Size: 685 3223
BstBI TT'CG_AA
Cuts at: 2269 2269
Size: 3908
BstXI CCAn_nnnn'nTGG
Cuts at: 636 636
Size: 3908
BstYI r'GATC_y
Cuts at: 1 381 398 933 1400 1423 1758 2004 3016
Size: 380 17 535 467 23 335 246 1012
Cuts at: 3016 3102 3114 3460 1
Size: 86 12 346 449
Fragments arranged by size:
1012 535 467 449 380 346 335 246
86 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 361 841 361
Size: 480 3428
BtgI C'CryG_G
Cuts at: 107 275 766 107
Size: 168 491 3249
Fragments arranged by size:
3249 491 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 295 1858 2039 2202 295
Size: 1563 181 163 2001
Fragments arranged by size:
2001 1563 181 163
BtsI GCAGTG_nn'
Cuts at: 1151 1151
Size: 3908
DraI TTT'AAA
Cuts at: 907 907
Size: 3908
DrdI GACnn_nn'nnGTC
Cuts at: 1614 2472 1614
Size: 858 3050
EaeI y'GGCC_r
Cuts at: 263 899 1493 1667 2058 2085 3430 263
Size: 636 594 174 391 27 1345 741
Fragments arranged by size:
1345 741 636 594 391 174 27
EagI C'GGCC_G
Cuts at: 1493 1493
Size: 3908
EarI CTCTTCn'nnn_
Cuts at: 1931 2141 1931
Size: 210 3698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2116 2426 2572 3718 2116
Size: 310 146 1146 2306
Fragments arranged by size:
2306 1146 310 146
EcoICRI GAG'CTC
Cuts at: 920 920
Size: 3908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 436 1733 2165 2912 3180 373
Size: 63 1297 432 747 268 1101
Fragments arranged by size:
1297 1101 747 432 268 63
EcoO109I rG'GnC_Cy
Cuts at: 357 1047 357
Size: 690 3218
EcoRI G'AATT_C
Cuts at: 912 912
Size: 3908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1927 1927
Size: 3908
FspI TGC'GCA
Cuts at: 1689 1689
Size: 3908
HaeII r_GCGC'y
Cuts at: 209 259 289 731 828 1342 1590 2612 3591
Size: 50 30 442 97 514 248 1022 979
Cuts at: 3591 209
Size: 526
Fragments arranged by size:
1022 979 526 514 442 248 97 50
30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 366 398 1750 1780 1782 1812 366
Size: 32 1352 30 2 30 2462
Fragments arranged by size:
2462 1352 32 30 30 2
HincII GTy'rAC
Cuts at: 947 947
Size: 3908
KasI G'GCGC_C
Cuts at: 205 1338 1586 205
Size: 1133 248 2527
Fragments arranged by size:
2527 1133 248
KpnI G_GTAC'C
Cuts at: 585 928 585
Size: 343 3565
MluI A'CGCG_T
Cuts at: 3341 3341
Size: 3908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 744 2579 2763 744
Size: 1835 184 1889
Fragments arranged by size:
1889 1835 184
MscI TGG'CCA
Cuts at: 1669 1669
Size: 3908
MslI CAynn'nnrTG
Cuts at: 1897 2024 1897
Size: 127 3781
MspA1I CmG'CkG
Cuts at: 165 246 277 579 1019 1333 1693 2706 2951
Size: 81 31 302 440 314 360 1013 245
Cuts at: 2951 165
Size: 1122
Fragments arranged by size:
1122 1013 440 360 314 302 245 81
31
NaeI GCC'GGC
Cuts at: 210 270 2089 210
Size: 60 1819 2029
Fragments arranged by size:
2029 1819 60
NarI GG'CG_CC
Cuts at: 206 1339 1587 206
Size: 1133 248 2527
Fragments arranged by size:
2527 1133 248
NcoI C'CATG_G
Cuts at: 107 766 107
Size: 659 3249
NgoMIV G'CCGG_C
Cuts at: 208 268 2087 208
Size: 60 1819 2029
Fragments arranged by size:
2029 1819 60
NheI G'CTAG_C
Cuts at: 561 561
Size: 3908
NruI TCG'CGA
Cuts at: 482 482
Size: 3908
NspI r_CATG'y
Cuts at: 603 961 1461 2368 603
Size: 358 500 907 2143
Fragments arranged by size:
2143 907 500 358
PciI A'CATG_T
Cuts at: 2364 2364
Size: 3908
PflMI CCAn_nnn'nTGG
Cuts at: 312 897 312
Size: 585 3323
PfoI T'CCnGG_A
Cuts at: 497 497
Size: 3908
PmeI GTTT'AAAC
Cuts at: 907 907
Size: 3908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 499 531 3047 3079 499
Size: 32 2516 32 1328
Fragments arranged by size:
2516 1328 32 32
PpuMI rG'GwC_Cy
Cuts at: 357 357
Size: 3908
PstI C_TGCA'G
Cuts at: 251 407 955 1640 251
Size: 156 548 685 2519
Fragments arranged by size:
2519 685 548 156
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3908
PvuII CAG'CTG
Cuts at: 1333 1693 1333
Size: 360 3548
RsrII CG'GwC_CG
Cuts at: 2103 2103
Size: 3908
SacI G_AGCT'C
Cuts at: 922 922
Size: 3908
SacII CC_GC'GG
Cuts at: 278 278
Size: 3908
SalI G'TCGA_C
Cuts at: 945 945
Size: 3908
SapI GCTCTTCn'nnn_
Cuts at: 1931 2141 1931
Size: 210 3698
SbfI CC_TGCA'GG
Cuts at: 407 955 407
Size: 548 3360
ScaI AGT'ACT
Cuts at: 3425 3425
Size: 3908
SfcI C'TryA_G
Cuts at: 33 160 247 403 951 1636 2629 2820 33
Size: 127 87 156 548 685 993 191 1121
Fragments arranged by size:
1121 993 685 548 191 156 127 87
SfoI GGC'GCC
Cuts at: 207 1340 1588 207
Size: 1133 248 2527
Fragments arranged by size:
2527 1133 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
SmaI CCC'GGG
Cuts at: 155 930 155
Size: 775 3133
SmlI C'TyrA_G
Cuts at: 1063 1978 2470 2732 1063
Size: 915 492 262 2239
Fragments arranged by size:
2239 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3908
SphI G_CATG'C
Cuts at: 961 1461 961
Size: 500 3408
StyI C'CwwG_G
Cuts at: 107 766 1042 1396 107
Size: 659 276 354 2619
Fragments arranged by size:
2619 659 354 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 466 549 688 729 894 1491 1624 2083 2292
Size: 83 139 41 165 597 133 459 209
Cuts at: 2292 466
Size: 2082
Fragments arranged by size:
2082 597 459 209 165 139 133 83
41
TatI w'GTAC_w
Cuts at: 812 3423 812
Size: 2611 1297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 3876
Tth111I GACn'n_nGTC
Cuts at: 313 1705 313
Size: 1392 2516
XbaI T'CTAG_A
Cuts at: 939 939
Size: 3908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 438 543 633 693 1403 438
Size: 105 90 60 710 2943
Fragments arranged by size:
2943 710 105 90 60
XmaI C'CCGG_G
Cuts at: 153 928 153
Size: 775 3133
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NruI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI Bpu10I BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI