Restriction map for
pF1KE0118
(Circular) (Six-base) MAPSORT of: pF1KE0118.seq Check: 7153 from: 1 to: 3698
pF1KE0118 3698 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 736 736
Size: 3698
Acc65I G'GTAC_C
Cuts at: 714 714
Size: 3698
AclI AA'CG_TT
Cuts at: 3538 3538
Size: 3698
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1523 1955 2702 2970 646
Size: 877 432 747 268 1374
Fragments arranged by size:
1374 877 747 432 268
AflII C'TTAA_G
Cuts at: 540 540
Size: 3698
AflIII A'CryG_T
Cuts at: 1050 2154 3131 1050
Size: 1104 977 1617
Fragments arranged by size:
1617 1104 977
AgeI A'CCGG_T
Cuts at: 2086 2086
Size: 3698
AlwNI CAG_nnn'CTG
Cuts at: 1003 2570 1003
Size: 1567 2131
ApaLI G'TGCA_C
Cuts at: 2468 2468
Size: 3698
ApoI r'AATT_y
Cuts at: 686 702 3051 3307 686
Size: 16 2349 256 1077
Fragments arranged by size:
2349 1077 256 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 3698
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
AvaI C'yCGr_G
Cuts at: 718 718
Size: 3698
BamHI G'GATC_C
Cuts at: 723 723
Size: 3698
BanI G'GyrC_C
Cuts at: 714 1128 1376 1411 714
Size: 414 248 35 3001
Fragments arranged by size:
3001 414 248 35
BanII G_rGCy'C
Cuts at: 712 1742 712
Size: 1030 2668
BbeI G_GCGC'C
Cuts at: 1132 1380 1132
Size: 248 3450
BciVI GTATCCnnnnn_n'
Cuts at: 320 1596 2357 3698 320
Size: 1276 761 1341 320
Fragments arranged by size:
1341 1276 761 320
BclI T'GATC_A
Cuts at: 1218 1218
Size: 3698
BglII A'GATC_T
Cuts at: 1213 3250 1213
Size: 2037 1661
BlpI GC'TnA_GC
Cuts at: 810 810
Size: 3698
Bme1580I G_kGCm'C
Cuts at: 1323 1416 2472 1323
Size: 93 1056 2549
Fragments arranged by size:
2549 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 910 991 1326 3470 910
Size: 81 335 2144 1138
Fragments arranged by size:
2144 1138 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 3698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 586
Size: 3698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1753 2245 2543 874
Size: 879 492 298 2029
Fragments arranged by size:
2029 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3402 3402
Size: 3698
BsaAI yAC'GTr
Cuts at: 1051 1051
Size: 3698
BsaBI GATnn'nnATC
Cuts at: 6 1212 3249 6
Size: 1206 2037 455
Fragments arranged by size:
2037 1206 455
BsaHI Gr'CG_yC
Cuts at: 1129 1377 2079 3575 1129
Size: 248 702 1496 1252
Fragments arranged by size:
1496 1252 702 248
BsaWI w'CCGG_w
Cuts at: 887 1408 2086 2360 2507 2918 887
Size: 521 678 274 147 411 1667
Fragments arranged by size:
1667 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1123 1881 2458 2954 1123
Size: 758 577 496 1867
Fragments arranged by size:
1867 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1240 1286 2494 104
Size: 1136 46 1208 1308
Fragments arranged by size:
1308 1208 1136 46
BsiHKAI G_wGCw'C
Cuts at: 712 1490 1680 2472 712
Size: 778 190 792 1938
Fragments arranged by size:
1938 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 712 1323 1416 1490 1680 1742 2472 712
Size: 611 93 74 190 62 730 1938
Fragments arranged by size:
1938 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2918 2918
Size: 3698
BspHI T'CATG_A
Cuts at: 2874 2874
Size: 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 748 962 1264 1645 748
Size: 214 302 381 2801
Fragments arranged by size:
2801 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1990 2044 3528 3693 50
Size: 1940 54 1484 165 55
Fragments arranged by size:
1940 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1610 1610
Size: 3698
BsrFI r'CCGG_y
Cuts at: 1696 1877 2086 1696
Size: 181 209 3308
Fragments arranged by size:
3308 209 181
BssSI C'ACGA_G
Cuts at: 1969 2327 1969
Size: 358 3340
BstAPI GCAn_nnn'nTGC
Cuts at: 1075 1075
Size: 3698
BstBI TT'CG_AA
Cuts at: 2059 2059
Size: 3698
BstYI r'GATC_y
Cuts at: 1 723 1190 1213 1548 1794 2806 2892 2904
Size: 722 467 23 335 246 1012 86 12
Cuts at: 2904 3250 1
Size: 346 449
Fragments arranged by size:
1012 722 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3698
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1648 1829 1992 1648
Size: 181 163 3354
Fragments arranged by size:
3354 181 163
BtsI GCAGTG_nn'
Cuts at: 941 941
Size: 3698
DraI TTT'AAA
Cuts at: 697 697
Size: 3698
DraIII CAC_nnn'GTG
Cuts at: 424 424
Size: 3698
DrdI GACnn_nn'nnGTC
Cuts at: 434 1404 2262 434
Size: 970 858 1870
Fragments arranged by size:
1870 970 858
EaeI y'GGCC_r
Cuts at: 1283 1457 1848 1875 3220 1283
Size: 174 391 27 1345 1761
Fragments arranged by size:
1761 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1283 1283
Size: 3698
EarI CTCTTCn'nnn_
Cuts at: 1721 1931 1721
Size: 210 3488
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1906 2216 2362 3508 1906
Size: 310 146 1146 2096
Fragments arranged by size:
2096 1146 310 146
EcoICRI GAG'CTC
Cuts at: 710 710
Size: 3698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 646 1523 1955 2702 2970 586
Size: 60 877 432 747 268 1314
Fragments arranged by size:
1314 877 747 432 268 60
EcoO109I rG'GnC_Cy
Cuts at: 657 837 657
Size: 180 3518
EcoRI G'AATT_C
Cuts at: 702 702
Size: 3698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 392 588 1717 392
Size: 196 1129 2373
Fragments arranged by size:
2373 1129 196
FspI TGC'GCA
Cuts at: 1479 1479
Size: 3698
HaeII r_GCGC'y
Cuts at: 1132 1380 2402 3381 1132
Size: 248 1022 979 1449
Fragments arranged by size:
1449 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 279 311 1540 1570 1572 1602 279
Size: 32 1229 30 2 30 2375
Fragments arranged by size:
2375 1229 32 30 30 2
HincII GTy'rAC
Cuts at: 737 737
Size: 3698
KasI G'GCGC_C
Cuts at: 1128 1376 1128
Size: 248 3450
KpnI G_GTAC'C
Cuts at: 718 718
Size: 3698
MfeI C'AATT_G
Cuts at: 146 146
Size: 3698
MluI A'CGCG_T
Cuts at: 3131 3131
Size: 3698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 204 2369 2553 204
Size: 2165 184 1349
Fragments arranged by size:
2165 1349 184
MscI TGG'CCA
Cuts at: 1459 1459
Size: 3698
MslI CAynn'nnrTG
Cuts at: 1687 1814 1687
Size: 127 3571
MspA1I CmG'CkG
Cuts at: 809 1123 1483 2496 2741 809
Size: 314 360 1013 245 1766
Fragments arranged by size:
1766 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1879 1879
Size: 3698
NarI GG'CG_CC
Cuts at: 1129 1377 1129
Size: 248 3450
NcoI C'CATG_G
Cuts at: 107 107
Size: 3698
NgoMIV G'CCGG_C
Cuts at: 1877 1877
Size: 3698
NspI r_CATG'y
Cuts at: 751 1251 2158 751
Size: 500 907 2291
Fragments arranged by size:
2291 907 500
PciI A'CATG_T
Cuts at: 2154 2154
Size: 3698
PmeI GTTT'AAAC
Cuts at: 697 697
Size: 3698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 3666
PsiI TTA'TAA
Cuts at: 652 652
Size: 3698
PstI C_TGCA'G
Cuts at: 745 1430 745
Size: 685 3013
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3698
PvuII CAG'CTG
Cuts at: 1123 1483 1123
Size: 360 3338
RsrII CG'GwC_CG
Cuts at: 1893 1893
Size: 3698
SacI G_AGCT'C
Cuts at: 712 712
Size: 3698
SalI G'TCGA_C
Cuts at: 735 735
Size: 3698
SapI GCTCTTCn'nnn_
Cuts at: 1721 1931 1721
Size: 210 3488
SbfI CC_TGCA'GG
Cuts at: 745 745
Size: 3698
ScaI AGT'ACT
Cuts at: 3215 3215
Size: 3698
SfcI C'TryA_G
Cuts at: 33 741 1426 2419 2610 33
Size: 708 685 993 191 1121
Fragments arranged by size:
1121 993 708 685 191
SfoI GGC'GCC
Cuts at: 1130 1378 1130
Size: 248 3450
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
SmaI CCC'GGG
Cuts at: 720 720
Size: 3698
SmlI C'TyrA_G
Cuts at: 540 853 1768 2260 2522 540
Size: 313 915 492 262 1716
Fragments arranged by size:
1716 915 492 313 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3698
SphI G_CATG'C
Cuts at: 751 1251 751
Size: 500 3198
StyI C'CwwG_G
Cuts at: 107 624 832 1186 107
Size: 517 208 354 2619
Fragments arranged by size:
2619 517 354 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1281 1414 1873 2082 1281
Size: 133 459 209 2897
Fragments arranged by size:
2897 459 209 133
TatI w'GTAC_w
Cuts at: 3213 3213
Size: 3698
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 3666
Tth111I GACn'n_nGTC
Cuts at: 1495 1495
Size: 3698
XbaI T'CTAG_A
Cuts at: 729 729
Size: 3698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1193 1193
Size: 3698
XmaI C'CCGG_G
Cuts at: 718 718
Size: 3698
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI