Restriction map for
pF1KE0114
(Circular) (Six-base) MAPSORT of: pF1KE0114.seq Check: 6379 from: 1 to: 4037
pF1KE0114 4037 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AatII G_ACGT'C
Cuts at: 269 269
Size: 4037
AccI GT'mk_AC
Cuts at: 155 1075 155
Size: 920 3117
Acc65I G'GTAC_C
Cuts at: 1053 1053
Size: 4037
AclI AA'CG_TT
Cuts at: 3877 3877
Size: 4037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 634 958 1862 2294 3041 3309 598
Size: 36 324 904 432 747 268 1326
Fragments arranged by size:
1326 904 747 432 324 268 36
AflIII A'CryG_T
Cuts at: 1389 2493 3470 1389
Size: 1104 977 1956
Fragments arranged by size:
1956 1104 977
AgeI A'CCGG_T
Cuts at: 2425 2425
Size: 4037
AlwNI CAG_nnn'CTG
Cuts at: 573 630 894 1342 2909 573
Size: 57 264 448 1567 1701
Fragments arranged by size:
1701 1567 448 264 57
ApaLI G'TGCA_C
Cuts at: 549 2807 549
Size: 2258 1779
ApoI r'AATT_y
Cuts at: 1041 3390 3646 1041
Size: 2349 256 1432
Fragments arranged by size:
2349 1432 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
AvaI C'yCGr_G
Cuts at: 1057 1057
Size: 4037
BamHI G'GATC_C
Cuts at: 1062 1062
Size: 4037
BanI G'GyrC_C
Cuts at: 111 1053 1467 1715 1750 111
Size: 942 414 248 35 2398
Fragments arranged by size:
2398 942 414 248 35
BanII G_rGCy'C
Cuts at: 350 1051 2081 350
Size: 701 1030 2306
Fragments arranged by size:
2306 1030 701
BbeI G_GCGC'C
Cuts at: 115 1471 1719 115
Size: 1356 248 2433
Fragments arranged by size:
2433 1356 248
BbsI GAAGACnn'nnnn_
Cuts at: 261 261
Size: 4037
BbvCI CC'TCA_GC
Cuts at: 812 812
Size: 4037
BciVI GTATCCnnnnn_n'
Cuts at: 1935 2696 4037 1935
Size: 761 1341 1935
Fragments arranged by size:
1935 1341 761
BclI T'GATC_A
Cuts at: 1557 1557
Size: 4037
BglII A'GATC_T
Cuts at: 1552 3589 1552
Size: 2037 2000
BlpI GC'TnA_GC
Cuts at: 1149 1149
Size: 4037
Bme1580I G_kGCm'C
Cuts at: 553 1662 1755 2811 553
Size: 1109 93 1056 1779
Fragments arranged by size:
1779 1109 1056 93
BmgBI CAC'GTC
Cuts at: 255 447 255
Size: 192 3845
BmrI ACTGGGnnnn_n'
Cuts at: 1249 1330 1665 3809 1249
Size: 81 335 2144 1477
Fragments arranged by size:
2144 1477 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 565 334
Size: 231 3806
Bpu10I CC'TnA_GC
Cuts at: 344 812 344
Size: 468 3569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 2092 2584 2882 1213
Size: 879 492 298 2368
Fragments arranged by size:
2368 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3741 3741
Size: 4037
BsaAI yAC'GTr
Cuts at: 1390 1390
Size: 4037
BsaBI GATnn'nnATC
Cuts at: 6 1551 3588 6
Size: 1545 2037 455
Fragments arranged by size:
2037 1545 455
BsaHI Gr'CG_yC
Cuts at: 112 266 1468 1716 2418 3914 112
Size: 154 1202 248 702 1496 235
Fragments arranged by size:
1496 1202 702 248 235 154
BsaWI w'CCGG_w
Cuts at: 1226 1747 2425 2699 2846 3257 1226
Size: 521 678 274 147 411 2006
Fragments arranged by size:
2006 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 324 324
Size: 4037
BseYI C'CCAG_C
Cuts at: 653 800 887 932 1024 1462 2220 2797 3293
Size: 147 87 45 92 438 758 577 496
Cuts at: 3293 653
Size: 1397
Fragments arranged by size:
1397 758 577 496 438 147 92 87
45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 586 511
Size: 75 3962
BsiEI CG_ry'CG
Cuts at: 104 1579 1625 2833 104
Size: 1475 46 1208 1308
Fragments arranged by size:
1475 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 350 553 745 1051 1829 2019 2811 350
Size: 203 192 306 778 190 792 1576
Fragments arranged by size:
1576 792 778 306 203 192 190
BsmI GAATG_Cn'
Cuts at: 626 626
Size: 4037
BsmBI CGTCTCn'nnnn_
Cuts at: 245 853 245
Size: 608 3429
Bsp1286I G_dGCh'C
Cuts at: 350 553 745 1051 1662 1755 1829 2019 2081
Size: 203 192 306 611 93 74 190 62
Cuts at: 2081 2811 350
Size: 730 1576
Fragments arranged by size:
1576 730 611 306 203 192 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3257 3257
Size: 4037
BspHI T'CATG_A
Cuts at: 3213 3213
Size: 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1087 1301 1603 1984 1087
Size: 214 302 381 3140
Fragments arranged by size:
3140 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2329 2383 3867 4032 50
Size: 2279 54 1484 165 55
Fragments arranged by size:
2279 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1949 1949
Size: 4037
BsrFI r'CCGG_y
Cuts at: 2035 2216 2425 2035
Size: 181 209 3647
Fragments arranged by size:
3647 209 181
BssSI C'ACGA_G
Cuts at: 385 2308 2666 385
Size: 1923 358 1756
Fragments arranged by size:
1923 1756 358
BstAPI GCAn_nnn'nTGC
Cuts at: 573 1414 573
Size: 841 3196
BstBI TT'CG_AA
Cuts at: 2398 2398
Size: 4037
BstYI r'GATC_y
Cuts at: 1 569 1062 1529 1552 1887 2133 3145 3231
Size: 568 493 467 23 335 246 1012 86
Cuts at: 3231 3243 3589 1
Size: 12 346 449
Fragments arranged by size:
1012 568 493 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 908 908
Size: 4037
BtgI C'CryG_G
Cuts at: 107 683 107
Size: 576 3461
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1987 2168 2331 1987
Size: 181 163 3693
Fragments arranged by size:
3693 181 163
BtsI GCAGTG_nn'
Cuts at: 211 662 1280 211
Size: 451 618 2968
Fragments arranged by size:
2968 618 451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 828 863 828
Size: 35 4002
DraI TTT'AAA
Cuts at: 1036 1036
Size: 4037
DrdI GACnn_nn'nnGTC
Cuts at: 1743 2601 1743
Size: 858 3179
EaeI y'GGCC_r
Cuts at: 410 473 680 1622 1796 2187 2214 3559 410
Size: 63 207 942 174 391 27 1345 888
Fragments arranged by size:
1345 942 888 391 207 174 63 27
EagI C'GGCC_G
Cuts at: 1622 1622
Size: 4037
EarI CTCTTCn'nnn_
Cuts at: 610 2060 2270 610
Size: 1450 210 2377
Fragments arranged by size:
2377 1450 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2245 2555 2701 3847 2245
Size: 310 146 1146 2435
Fragments arranged by size:
2435 1146 310 146
EcoICRI GAG'CTC
Cuts at: 348 1049 348
Size: 701 3336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 565 598 634 958 1862 2294 3041 3309
Size: 231 33 36 324 904 432 747 268
Cuts at: 3309 334
Size: 1062
Fragments arranged by size:
1062 904 747 432 324 268 231 36
33
EcoNI CCTnn'n_nnAGG
Cuts at: 634 823 634
Size: 189 3848
EcoO109I rG'GnC_Cy
Cuts at: 627 816 953 1176 627
Size: 189 137 223 3488
Fragments arranged by size:
3488 223 189 137
EcoRI G'AATT_C
Cuts at: 1041 1041
Size: 4037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 198 2056 198
Size: 1858 2179
FspI TGC'GCA
Cuts at: 361 1818 361
Size: 1457 2580
FspAI rTGC'GCAy
Cuts at: 361 361
Size: 4037
HaeII r_GCGC'y
Cuts at: 115 835 1031 1471 1719 2741 3720 115
Size: 720 196 440 248 1022 979 432
Fragments arranged by size:
1022 979 720 440 432 248 196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 727 759 1879 1909 1911 1941 727
Size: 32 1120 30 2 30 2823
Fragments arranged by size:
2823 1120 32 30 30 2
HincII GTy'rAC
Cuts at: 870 1076 870
Size: 206 3831
KasI G'GCGC_C
Cuts at: 111 1467 1715 111
Size: 1356 248 2433
Fragments arranged by size:
2433 1356 248
KpnI G_GTAC'C
Cuts at: 1057 1057
Size: 4037
MluI A'CGCG_T
Cuts at: 3470 3470
Size: 4037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 373 2708 2892 373
Size: 2335 184 1518
Fragments arranged by size:
2335 1518 184
MscI TGG'CCA
Cuts at: 682 1798 682
Size: 1116 2921
MslI CAynn'nnrTG
Cuts at: 556 562 2026 2153 556
Size: 6 1464 127 2440
Fragments arranged by size:
2440 1464 127 6
MspA1I CmG'CkG
Cuts at: 225 800 811 936 1148 1462 1822 2835 3080
Size: 575 11 125 212 314 360 1013 245
Cuts at: 3080 225
Size: 1182
Fragments arranged by size:
1182 1013 575 360 314 245 212 125
11
NaeI GCC'GGC
Cuts at: 2218 2218
Size: 4037
NarI GG'CG_CC
Cuts at: 112 1468 1716 112
Size: 1356 248 2433
Fragments arranged by size:
2433 1356 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4037
NgoMIV G'CCGG_C
Cuts at: 2216 2216
Size: 4037
NspI r_CATG'y
Cuts at: 366 1090 1590 2497 366
Size: 724 500 907 1906
Fragments arranged by size:
1906 907 724 500
PasI CC'CwG_GG
Cuts at: 792 792
Size: 4037
PciI A'CATG_T
Cuts at: 2493 2493
Size: 4037
PflMI CCAn_nnn'nTGG
Cuts at: 668 729 894 668
Size: 61 165 3811
Fragments arranged by size:
3811 165 61
PfoI T'CCnGG_A
Cuts at: 314 722 314
Size: 408 3629
PmeI GTTT'AAAC
Cuts at: 1036 1036
Size: 4037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 4005
PpuMI rG'GwC_Cy
Cuts at: 627 953 627
Size: 326 3711
PshAI GACnn'nnGTC
Cuts at: 865 865
Size: 4037
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 169 201 169
Size: 32 4005
PstI C_TGCA'G
Cuts at: 704 827 1084 1769 704
Size: 123 257 685 2972
Fragments arranged by size:
2972 685 257 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4037
PvuII CAG'CTG
Cuts at: 936 1462 1822 936
Size: 526 360 3151
Fragments arranged by size:
3151 526 360
RsrII CG'GwC_CG
Cuts at: 2232 2232
Size: 4037
SacI G_AGCT'C
Cuts at: 350 1051 350
Size: 701 3336
SalI G'TCGA_C
Cuts at: 1074 1074
Size: 4037
SapI GCTCTTCn'nnn_
Cuts at: 2060 2270 2060
Size: 210 3827
SbfI CC_TGCA'GG
Cuts at: 1084 1084
Size: 4037
ScaI AGT'ACT
Cuts at: 3554 3554
Size: 4037
SfcI C'TryA_G
Cuts at: 33 700 823 1012 1080 1765 2758 2949 33
Size: 667 123 189 68 685 993 191 1121
Fragments arranged by size:
1121 993 685 667 191 189 123 68
SfoI GGC'GCC
Cuts at: 113 1469 1717 113
Size: 1356 248 2433
Fragments arranged by size:
2433 1356 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
SmaI CCC'GGG
Cuts at: 1059 1059
Size: 4037
SmlI C'TyrA_G
Cuts at: 1192 2107 2599 2861 1192
Size: 915 492 262 2368
Fragments arranged by size:
2368 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4037
SphI G_CATG'C
Cuts at: 1090 1590 1090
Size: 500 3537
StyI C'CwwG_G
Cuts at: 107 983 1171 1525 107
Size: 876 188 354 2619
Fragments arranged by size:
2619 876 354 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 437 667 1620 1753 2212 2421 437
Size: 230 953 133 459 209 2053
Fragments arranged by size:
2053 953 459 230 209 133
TatI w'GTAC_w
Cuts at: 3552 3552
Size: 4037
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 291 323 3554 3586 291
Size: 32 3231 32 742
Fragments arranged by size:
3231 742 32 32
Tth111I GACn'n_nGTC
Cuts at: 1834 1834
Size: 4037
XbaI T'CTAG_A
Cuts at: 1068 1068
Size: 4037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1532 1532
Size: 4037
XmaI C'CCGG_G
Cuts at: 1057 1057
Size: 4037
XmnI GAAnn'nnTTC
Cuts at: 181 181
Size: 4037
ZraI GAC'GTC
Cuts at: 267 267
Size: 4037
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsaXI
BsiWI BsrGI BssHII BstEII BstXI BstZ17I ClaI DraIII
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI