Restriction map for
pF1KE0113
(Circular) (Six-base) MAPSORT of: pF1KE0113.seq Check: 6005 from: 1 to: 3377
pF1KE0113 3377 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 415 415
Size: 3377
Acc65I G'GTAC_C
Cuts at: 393 393
Size: 3377
AclI AA'CG_TT
Cuts at: 3217 3217
Size: 3377
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1202 1634 2381 2649 1202
Size: 432 747 268 1930
Fragments arranged by size:
1930 747 432 268
AflIII A'CryG_T
Cuts at: 729 1833 2810 729
Size: 1104 977 1296
Fragments arranged by size:
1296 1104 977
AgeI A'CCGG_T
Cuts at: 1765 1765
Size: 3377
AleI CACnn'nnGTG
Cuts at: 139 139
Size: 3377
AlwNI CAG_nnn'CTG
Cuts at: 682 2249 682
Size: 1567 1810
ApaLI G'TGCA_C
Cuts at: 2147 2147
Size: 3377
ApoI r'AATT_y
Cuts at: 381 2730 2986 381
Size: 2349 256 772
Fragments arranged by size:
2349 772 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3377
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3377
AvaI C'yCGr_G
Cuts at: 397 397
Size: 3377
BamHI G'GATC_C
Cuts at: 402 402
Size: 3377
BanI G'GyrC_C
Cuts at: 393 807 1055 1090 393
Size: 414 248 35 2680
Fragments arranged by size:
2680 414 248 35
BanII G_rGCy'C
Cuts at: 391 1421 391
Size: 1030 2347
BbeI G_GCGC'C
Cuts at: 811 1059 811
Size: 248 3129
BciVI GTATCCnnnnn_n'
Cuts at: 1275 2036 3377 1275
Size: 761 1341 1275
Fragments arranged by size:
1341 1275 761
BclI T'GATC_A
Cuts at: 897 897
Size: 3377
BglI GCCn_nnn'nGGC
Cuts at: 175 184 175
Size: 9 3368
BglII A'GATC_T
Cuts at: 892 2929 892
Size: 2037 1340
BlpI GC'TnA_GC
Cuts at: 489 489
Size: 3377
Bme1580I G_kGCm'C
Cuts at: 1002 1095 2151 1002
Size: 93 1056 2228
Fragments arranged by size:
2228 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 589 670 1005 3149 589
Size: 81 335 2144 817
Fragments arranged by size:
2144 817 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1744 1744
Size: 3377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1432 1924 2222 553
Size: 879 492 298 1708
Fragments arranged by size:
1708 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 265 3081 265
Size: 2816 561
BsaAI yAC'GTr
Cuts at: 730 730
Size: 3377
BsaBI GATnn'nnATC
Cuts at: 6 891 2928 6
Size: 885 2037 455
Fragments arranged by size:
2037 885 455
BsaHI Gr'CG_yC
Cuts at: 808 1056 1758 3254 808
Size: 248 702 1496 931
Fragments arranged by size:
1496 931 702 248
BsaWI w'CCGG_w
Cuts at: 566 1087 1765 2039 2186 2597 566
Size: 521 678 274 147 411 1346
Fragments arranged by size:
1346 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 262 262
Size: 3377
BseYI C'CCAG_C
Cuts at: 802 1560 2137 2633 802
Size: 758 577 496 1546
Fragments arranged by size:
1546 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 316
Size: 3377
BsiEI CG_ry'CG
Cuts at: 104 919 965 2173 104
Size: 815 46 1208 1308
Fragments arranged by size:
1308 1208 815 46
BsiHKAI G_wGCw'C
Cuts at: 391 1169 1359 2151 391
Size: 778 190 792 1617
Fragments arranged by size:
1617 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 391 1002 1095 1169 1359 1421 2151 391
Size: 611 93 74 190 62 730 1617
Fragments arranged by size:
1617 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2597 2597
Size: 3377
BspHI T'CATG_A
Cuts at: 2553 2553
Size: 3377
BspMI ACCTGCnnnn'nnnn_
Cuts at: 221 427 641 943 1324 221
Size: 206 214 302 381 2274
Fragments arranged by size:
2274 381 302 214 206
BsrBI CCG'CTC
Cuts at: 50 1669 1723 3207 3372 50
Size: 1619 54 1484 165 55
Fragments arranged by size:
1619 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 164 1289 164
Size: 1125 2252
BsrFI r'CCGG_y
Cuts at: 1375 1556 1765 1375
Size: 181 209 2987
Fragments arranged by size:
2987 209 181
BssSI C'ACGA_G
Cuts at: 1648 2006 1648
Size: 358 3019
BstAPI GCAn_nnn'nTGC
Cuts at: 754 754
Size: 3377
BstBI TT'CG_AA
Cuts at: 1738 1738
Size: 3377
BstXI CCAn_nnnn'nTGG
Cuts at: 141 141
Size: 3377
BstYI r'GATC_y
Cuts at: 1 402 869 892 1227 1473 2485 2571 2583
Size: 401 467 23 335 246 1012 86 12
Cuts at: 2583 2929 1
Size: 346 449
Fragments arranged by size:
1012 467 449 401 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 134 366 134
Size: 232 3145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 156 1327 1508 1671 156
Size: 1171 181 163 1862
Fragments arranged by size:
1862 1171 181 163
BtsI GCAGTG_nn'
Cuts at: 620 620
Size: 3377
DraI TTT'AAA
Cuts at: 376 376
Size: 3377
DrdI GACnn_nn'nnGTC
Cuts at: 1083 1941 1083
Size: 858 2519
EaeI y'GGCC_r
Cuts at: 305 962 1136 1527 1554 2899 305
Size: 657 174 391 27 1345 783
Fragments arranged by size:
1345 783 657 391 174 27
EagI C'GGCC_G
Cuts at: 962 962
Size: 3377
EarI CTCTTCn'nnn_
Cuts at: 1400 1610 1400
Size: 210 3167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1585 1895 2041 3187 1585
Size: 310 146 1146 1775
Fragments arranged by size:
1775 1146 310 146
EcoICRI GAG'CTC
Cuts at: 389 389
Size: 3377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1202 1634 2381 2649 1202
Size: 432 747 268 1930
Fragments arranged by size:
1930 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 185 516 185
Size: 331 3046
EcoRI G'AATT_C
Cuts at: 381 381
Size: 3377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 300 1396 300
Size: 1096 2281
FspI TGC'GCA
Cuts at: 1158 1158
Size: 3377
HaeII r_GCGC'y
Cuts at: 811 1059 2081 3060 811
Size: 248 1022 979 1128
Fragments arranged by size:
1128 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1219 1249 1251 1281 1219
Size: 30 2 30 3315
Fragments arranged by size:
3315 30 30 2
HincII GTy'rAC
Cuts at: 416 416
Size: 3377
KasI G'GCGC_C
Cuts at: 807 1055 807
Size: 248 3129
KpnI G_GTAC'C
Cuts at: 397 397
Size: 3377
MluI A'CGCG_T
Cuts at: 2810 2810
Size: 3377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2048 2232 2048
Size: 184 3193
MscI TGG'CCA
Cuts at: 1138 1138
Size: 3377
MslI CAynn'nnrTG
Cuts at: 139 1366 1493 139
Size: 1227 127 2023
Fragments arranged by size:
2023 1227 127
MspA1I CmG'CkG
Cuts at: 488 802 1162 2175 2420 488
Size: 314 360 1013 245 1445
Fragments arranged by size:
1445 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1558 1558
Size: 3377
NarI GG'CG_CC
Cuts at: 808 1056 808
Size: 248 3129
NgoMIV G'CCGG_C
Cuts at: 1556 1556
Size: 3377
NspI r_CATG'y
Cuts at: 430 930 1837 430
Size: 500 907 1970
Fragments arranged by size:
1970 907 500
PasI CC'CwG_GG
Cuts at: 180 180
Size: 3377
PciI A'CATG_T
Cuts at: 1833 1833
Size: 3377
PmeI GTTT'AAAC
Cuts at: 376 376
Size: 3377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2516 2548 2516
Size: 32 3345
PstI C_TGCA'G
Cuts at: 224 424 1109 224
Size: 200 685 2492
Fragments arranged by size:
2492 685 200
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3377
PvuII CAG'CTG
Cuts at: 802 1162 802
Size: 360 3017
RsrII CG'GwC_CG
Cuts at: 1572 1572
Size: 3377
SacI G_AGCT'C
Cuts at: 391 391
Size: 3377
SalI G'TCGA_C
Cuts at: 414 414
Size: 3377
SapI GCTCTTCn'nnn_
Cuts at: 1400 1610 1400
Size: 210 3167
SbfI CC_TGCA'GG
Cuts at: 424 424
Size: 3377
ScaI AGT'ACT
Cuts at: 2894 2894
Size: 3377
SfcI C'TryA_G
Cuts at: 33 220 420 1105 2098 2289 33
Size: 187 200 685 993 191 1121
Fragments arranged by size:
1121 993 685 200 191 187
SfiI GGCCn_nnn'nGGCC
Cuts at: 184 184
Size: 3377
SfoI GGC'GCC
Cuts at: 809 1057 809
Size: 248 3129
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3377
SmaI CCC'GGG
Cuts at: 399 399
Size: 3377
SmlI C'TyrA_G
Cuts at: 532 1447 1939 2201 532
Size: 915 492 262 1708
Fragments arranged by size:
1708 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3377
SphI G_CATG'C
Cuts at: 430 930 430
Size: 500 2877
StyI C'CwwG_G
Cuts at: 275 308 511 865 275
Size: 33 203 354 2787
Fragments arranged by size:
2787 354 203 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 960 1093 1552 1761 960
Size: 133 459 209 2576
Fragments arranged by size:
2576 459 209 133
TatI w'GTAC_w
Cuts at: 2892 2892
Size: 3377
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 305 337 2894 2926 305
Size: 32 2557 32 756
Fragments arranged by size:
2557 756 32 32
Tth111I GACn'n_nGTC
Cuts at: 1174 1174
Size: 3377
XbaI T'CTAG_A
Cuts at: 408 408
Size: 3377
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 138 177 872 114
Size: 24 39 695 2619
Fragments arranged by size:
2619 695 39 24
XmaI C'CCGG_G
Cuts at: 397 397
Size: 3377
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI