Restriction map for
pF1KE0108
(Circular) (Six-base) MAPSORT of: pF1KE0108.seq Check: 2015 from: 1 to: 4067
pF1KE0108 4067 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AatII G_ACGT'C
Cuts at: 399 908 399
Size: 509 3558
AccI GT'mk_AC
Cuts at: 1105 1105
Size: 4067
Acc65I G'GTAC_C
Cuts at: 121 1083 121
Size: 962 3105
AclI AA'CG_TT
Cuts at: 3907 3907
Size: 4067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1021 1892 2324 3071 3339 718
Size: 303 871 432 747 268 1446
Fragments arranged by size:
1446 871 747 432 303 268
AflIII A'CryG_T
Cuts at: 1419 2523 3500 1419
Size: 1104 977 1986
Fragments arranged by size:
1986 1104 977
AgeI A'CCGG_T
Cuts at: 113 2455 113
Size: 2342 1725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 308 846 308
Size: 538 3529
AlwNI CAG_nnn'CTG
Cuts at: 1372 2939 1372
Size: 1567 2500
ApaLI G'TGCA_C
Cuts at: 2837 2837
Size: 4067
ApoI r'AATT_y
Cuts at: 252 336 443 1071 3420 3676 252
Size: 84 107 628 2349 256 643
Fragments arranged by size:
2349 643 628 256 107 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4067
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 1087 1087
Size: 4067
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 854 887 854
Size: 33 4034
BamHI G'GATC_C
Cuts at: 995 1092 995
Size: 97 3970
BanI G'GyrC_C
Cuts at: 121 292 1083 1497 1745 1780 121
Size: 171 791 414 248 35 2408
Fragments arranged by size:
2408 791 414 248 171 35
BanII G_rGCy'C
Cuts at: 1081 2111 1081
Size: 1030 3037
BbeI G_GCGC'C
Cuts at: 1501 1749 1501
Size: 248 3819
BbsI GAAGACnn'nnnn_
Cuts at: 279 279
Size: 4067
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2726 4067 1965
Size: 761 1341 1965
Fragments arranged by size:
1965 1341 761
BclI T'GATC_A
Cuts at: 1587 1587
Size: 4067
BglII A'GATC_T
Cuts at: 1582 3619 1582
Size: 2037 2030
BlpI GC'TnA_GC
Cuts at: 595 1179 595
Size: 584 3483
Bme1580I G_kGCm'C
Cuts at: 297 1692 1785 2841 297
Size: 1395 93 1056 1523
Fragments arranged by size:
1523 1395 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 705 829 1279 1360 1695 3839 705
Size: 124 450 81 335 2144 933
Fragments arranged by size:
2144 933 450 335 124 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1036 2434 1036
Size: 1398 2669
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 839 265
Size: 574 3493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1243 2122 2614 2912 562
Size: 681 879 492 298 1717
Fragments arranged by size:
1717 879 681 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 576 3771 576
Size: 3195 872
BsaAI yAC'GTr
Cuts at: 1420 1420
Size: 4067
BsaBI GATnn'nnATC
Cuts at: 6 1581 3618 6
Size: 1575 2037 455
Fragments arranged by size:
2037 1575 455
BsaHI Gr'CG_yC
Cuts at: 396 905 1498 1746 2448 3944 396
Size: 509 593 248 702 1496 519
Fragments arranged by size:
1496 702 593 519 509 248
BsaWI w'CCGG_w
Cuts at: 113 1256 1777 2455 2729 2876 3287 113
Size: 1143 521 678 274 147 411 893
Fragments arranged by size:
1143 893 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 268
Size: 4067
BseYI C'CCAG_C
Cuts at: 296 1492 2250 2827 3323 296
Size: 1196 758 577 496 1040
Fragments arranged by size:
1196 1040 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 724
Size: 4067
BsiEI CG_ry'CG
Cuts at: 104 1609 1655 2863 104
Size: 1505 46 1208 1308
Fragments arranged by size:
1505 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1081 1859 2049 2841 1081
Size: 778 190 792 2307
Fragments arranged by size:
2307 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 387 387
Size: 4067
Bsp1286I G_dGCh'C
Cuts at: 297 1081 1692 1785 1859 2049 2111 2841 297
Size: 784 611 93 74 190 62 730 1523
Fragments arranged by size:
1523 784 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3287 3287
Size: 4067
BspHI T'CATG_A
Cuts at: 3243 3243
Size: 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1117 1331 1633 2014 1117
Size: 214 302 381 3170
Fragments arranged by size:
3170 381 302 214
BsrBI CCG'CTC
Cuts at: 50 987 2359 2413 3897 4062 50
Size: 937 1372 54 1484 165 55
Fragments arranged by size:
1484 1372 937 165 55 54
BsrDI GCAATG_nn'
Cuts at: 183 835 1979 183
Size: 652 1144 2271
Fragments arranged by size:
2271 1144 652
BsrFI r'CCGG_y
Cuts at: 113 2065 2246 2455 113
Size: 1952 181 209 1725
Fragments arranged by size:
1952 1725 209 181
BssSI C'ACGA_G
Cuts at: 2338 2696 2338
Size: 358 3709
BstAPI GCAn_nnn'nTGC
Cuts at: 1444 1444
Size: 4067
BstBI TT'CG_AA
Cuts at: 447 2428 447
Size: 1981 2086
BstXI CCAn_nnnn'nTGG
Cuts at: 289 289
Size: 4067
BstYI r'GATC_y
Cuts at: 1 995 1092 1559 1582 1917 2163 3175 3261
Size: 994 97 467 23 335 246 1012 86
Cuts at: 3261 3273 3619 1
Size: 12 346 449
Fragments arranged by size:
1012 994 467 449 346 335 246 97
86 23 12
BtgI C'CryG_G
Cuts at: 140 1055 140
Size: 915 3152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 333 2017 2198 2361 333
Size: 1684 181 163 2039
Fragments arranged by size:
2039 1684 181 163
BtsI GCAGTG_nn'
Cuts at: 830 1310 830
Size: 480 3587
DraI TTT'AAA
Cuts at: 1066 1066
Size: 4067
DrdI GACnn_nn'nnGTC
Cuts at: 1773 2631 1773
Size: 858 3209
EaeI y'GGCC_r
Cuts at: 1652 1826 2217 2244 3589 1652
Size: 174 391 27 1345 2130
Fragments arranged by size:
2130 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4067
EarI CTCTTCn'nnn_
Cuts at: 369 684 2090 2300 369
Size: 315 1406 210 2136
Fragments arranged by size:
2136 1406 315 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 783 2275 2585 2731 3877 783
Size: 1492 310 146 1146 973
Fragments arranged by size:
1492 1146 973 310 146
EcoICRI GAG'CTC
Cuts at: 1079 1079
Size: 4067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 718 839 1021 1892 2324 3071 3339 265
Size: 453 121 182 871 432 747 268 993
Fragments arranged by size:
993 871 747 453 432 268 182 121
EcoO109I rG'GnC_Cy
Cuts at: 1206 1206
Size: 4067
EcoRI G'AATT_C
Cuts at: 336 443 1071 336
Size: 107 628 3332
Fragments arranged by size:
3332 628 107
EcoRV GAT'ATC
Cuts at: 216 216
Size: 4067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 4067
FspI TGC'GCA
Cuts at: 1848 1848
Size: 4067
HaeII r_GCGC'y
Cuts at: 1501 1749 2771 3750 1501
Size: 248 1022 979 1818
Fragments arranged by size:
1818 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 311 343 744 776 1909 1939 1941 1971 311
Size: 32 401 32 1133 30 2 30 2407
Fragments arranged by size:
2407 1133 401 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1106 1106
Size: 4067
KasI G'GCGC_C
Cuts at: 1497 1745 1497
Size: 248 3819
KpnI G_GTAC'C
Cuts at: 125 1087 125
Size: 962 3105
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 354 936 2738 2922 354
Size: 582 1802 184 1499
Fragments arranged by size:
1802 1499 582 184
MscI TGG'CCA
Cuts at: 1828 1828
Size: 4067
MslI CAynn'nnrTG
Cuts at: 2056 2183 2056
Size: 127 3940
MspA1I CmG'CkG
Cuts at: 1178 1492 1852 2865 3110 1178
Size: 314 360 1013 245 2135
Fragments arranged by size:
2135 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2248 2248
Size: 4067
NarI GG'CG_CC
Cuts at: 1498 1746 1498
Size: 248 3819
NgoMIV G'CCGG_C
Cuts at: 2246 2246
Size: 4067
NspI r_CATG'y
Cuts at: 1120 1620 2527 1120
Size: 500 907 2660
Fragments arranged by size:
2660 907 500
PciI A'CATG_T
Cuts at: 2523 2523
Size: 4067
PfoI T'CCnGG_A
Cuts at: 816 816
Size: 4067
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 4035
PstI C_TGCA'G
Cuts at: 938 1114 1799 938
Size: 176 685 3206
Fragments arranged by size:
3206 685 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 1492 1852 1492
Size: 360 3707
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 1081 1081
Size: 4067
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 3584 3584
Size: 4067
SfcI C'TryA_G
Cuts at: 33 934 1110 1795 2788 2979 33
Size: 901 176 685 993 191 1121
Fragments arranged by size:
1121 993 901 685 191 176
SfoI GGC'GCC
Cuts at: 1499 1747 1499
Size: 248 3819
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 577 1222 2137 2629 2891 577
Size: 645 915 492 262 1753
Fragments arranged by size:
1753 915 645 492 262
SpeI A'CTAG_T
Cuts at: 67 355 67
Size: 288 3779
SphI G_CATG'C
Cuts at: 1120 1620 1120
Size: 500 3567
SspI AAT'ATT
Cuts at: 505 505
Size: 4067
StuI AGG'CCT
Cuts at: 695 695
Size: 4067
StyI C'CwwG_G
Cuts at: 1201 1555 1201
Size: 354 3713
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1650 1783 2242 2451 1650
Size: 133 459 209 3266
Fragments arranged by size:
3266 459 209 133
TatI w'GTAC_w
Cuts at: 3582 3582
Size: 4067
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 4035
Tth111I GACn'n_nGTC
Cuts at: 1864 1864
Size: 4067
XbaI T'CTAG_A
Cuts at: 1098 1098
Size: 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 337 1562 337
Size: 1225 2842
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
ZraI GAC'GTC
Cuts at: 397 906 397
Size: 509 3558
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI XmnI