Restriction map for
pF1KE0107
(Circular) (Six-base) MAPSORT of: pF1KE0107.seq Check: 7682 from: 1 to: 3962
pF1KE0107 3962 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AatII G_ACGT'C
Cuts at: 491 491
Size: 3962
AccI GT'mk_AC
Cuts at: 1000 1000
Size: 3962
Acc65I G'GTAC_C
Cuts at: 978 978
Size: 3962
AclI AA'CG_TT
Cuts at: 3802 3802
Size: 3962
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 647 1787 2219 2966 3234 172
Size: 475 1140 432 747 268 900
Fragments arranged by size:
1140 900 747 475 432 268
AflIII A'CryG_T
Cuts at: 424 682 1314 2418 3395 424
Size: 258 632 1104 977 991
Fragments arranged by size:
1104 991 977 632 258
AgeI A'CCGG_T
Cuts at: 2350 2350
Size: 3962
AleI CACnn'nnGTG
Cuts at: 532 532
Size: 3962
AlwNI CAG_nnn'CTG
Cuts at: 459 520 950 1267 2834 459
Size: 61 430 317 1567 1587
Fragments arranged by size:
1587 1567 430 317 61
ApaLI G'TGCA_C
Cuts at: 2732 2732
Size: 3962
ApoI r'AATT_y
Cuts at: 966 3315 3571 966
Size: 2349 256 1357
Fragments arranged by size:
2349 1357 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3962
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
AvaI C'yCGr_G
Cuts at: 845 982 845
Size: 137 3825
BamHI G'GATC_C
Cuts at: 987 987
Size: 3962
BanI G'GyrC_C
Cuts at: 978 1392 1640 1675 978
Size: 414 248 35 3265
Fragments arranged by size:
3265 414 248 35
BanII G_rGCy'C
Cuts at: 349 976 2006 349
Size: 627 1030 2305
Fragments arranged by size:
2305 1030 627
BbeI G_GCGC'C
Cuts at: 1396 1644 1396
Size: 248 3714
BbsI GAAGACnn'nnnn_
Cuts at: 274 274
Size: 3962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 892 926 892
Size: 34 3928
BciVI GTATCCnnnnn_n'
Cuts at: 793 1860 2621 3962 793
Size: 1067 761 1341 793
Fragments arranged by size:
1341 1067 793 761
BclI T'GATC_A
Cuts at: 1482 1482
Size: 3962
BglI GCCn_nnn'nGGC
Cuts at: 176 176
Size: 3962
BglII A'GATC_T
Cuts at: 1477 3514 1477
Size: 2037 1925
BlpI GC'TnA_GC
Cuts at: 1074 1074
Size: 3962
Bme1580I G_kGCm'C
Cuts at: 432 1587 1680 2736 432
Size: 1155 93 1056 1658
Fragments arranged by size:
1658 1155 1056 93
BmgBI CAC'GTC
Cuts at: 441 441
Size: 3962
BmrI ACTGGGnnnn_n'
Cuts at: 638 1174 1255 1590 3734 638
Size: 536 81 335 2144 866
Fragments arranged by size:
2144 866 536 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 3962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 205
Size: 3962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1138 2017 2509 2807 157
Size: 981 879 492 298 1312
Fragments arranged by size:
1312 981 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 855 3666 855
Size: 2811 1151
BsaAI yAC'GTr
Cuts at: 683 1315 683
Size: 632 3330
BsaBI GATnn'nnATC
Cuts at: 6 1476 3513 6
Size: 1470 2037 455
Fragments arranged by size:
2037 1470 455
BsaHI Gr'CG_yC
Cuts at: 145 488 1393 1641 2343 3839 145
Size: 343 905 248 702 1496 268
Fragments arranged by size:
1496 905 702 343 268 248
BsaWI w'CCGG_w
Cuts at: 632 1151 1672 2350 2624 2771 3182 632
Size: 519 521 678 274 147 411 1412
Fragments arranged by size:
1412 678 521 519 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 562 592 562
Size: 30 3932
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 205 348 202
Size: 3 143 3816
Fragments arranged by size:
3816 143 3
BseYI C'CCAG_C
Cuts at: 1387 2145 2722 3218 1387
Size: 758 577 496 2131
Fragments arranged by size:
2131 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 190 205 613 109
Size: 81 15 408 3458
Fragments arranged by size:
3458 408 81 15
BsiEI CG_ry'CG
Cuts at: 104 1504 1550 2758 104
Size: 1400 46 1208 1308
Fragments arranged by size:
1400 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 349 976 1754 1944 2736 349
Size: 627 778 190 792 1575
Fragments arranged by size:
1575 792 778 627 190
BsmBI CGTCTCn'nnnn_
Cuts at: 130 130
Size: 3962
Bsp1286I G_dGCh'C
Cuts at: 349 432 976 1587 1680 1754 1944 2006 2736
Size: 83 544 611 93 74 190 62 730
Cuts at: 2736 349
Size: 1575
Fragments arranged by size:
1575 730 611 544 190 93 83 74
62
BspEI T'CCGG_A
Cuts at: 3182 3182
Size: 3962
BspHI T'CATG_A
Cuts at: 263 3138 263
Size: 2875 1087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 707 1012 1226 1528 1909 707
Size: 305 214 302 381 2760
Fragments arranged by size:
2760 381 305 302 214
BsrBI CCG'CTC
Cuts at: 50 237 413 449 2254 2308 3792 3957 50
Size: 187 176 36 1805 54 1484 165 55
Fragments arranged by size:
1805 1484 187 176 165 55 54 36
BsrDI GCAATG_nn'
Cuts at: 1874 1874
Size: 3962
BsrFI r'CCGG_y
Cuts at: 1960 2141 2350 1960
Size: 181 209 3572
Fragments arranged by size:
3572 209 181
BssSI C'ACGA_G
Cuts at: 127 2233 2591 127
Size: 2106 358 1498
Fragments arranged by size:
2106 1498 358
BstAPI GCAn_nnn'nTGC
Cuts at: 459 1339 459
Size: 880 3082
BstBI TT'CG_AA
Cuts at: 2323 2323
Size: 3962
BstXI CCAn_nnnn'nTGG
Cuts at: 478 478
Size: 3962
BstYI r'GATC_y
Cuts at: 1 398 987 1454 1477 1812 2058 3070 3156
Size: 397 589 467 23 335 246 1012 86
Cuts at: 3156 3168 3514 1
Size: 12 346 449
Fragments arranged by size:
1012 589 467 449 397 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 291 471 560 107
Size: 184 180 89 3509
Fragments arranged by size:
3509 184 180 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 431 1912 2093 2256 431
Size: 1481 181 163 2137
Fragments arranged by size:
2137 1481 181 163
BtsI GCAGTG_nn'
Cuts at: 1205 1205
Size: 3962
DraI TTT'AAA
Cuts at: 961 961
Size: 3962
DraIII CAC_nnn'GTG
Cuts at: 213 228 213
Size: 15 3947
DrdI GACnn_nn'nnGTC
Cuts at: 144 1668 2526 144
Size: 1524 858 1580
Fragments arranged by size:
1580 1524 858
EaeI y'GGCC_r
Cuts at: 582 1547 1721 2112 2139 3484 582
Size: 965 174 391 27 1345 1060
Fragments arranged by size:
1345 1060 965 391 174 27
EagI C'GGCC_G
Cuts at: 1547 1547
Size: 3962
EarI CTCTTCn'nnn_
Cuts at: 148 1985 2195 148
Size: 1837 210 1915
Fragments arranged by size:
1915 1837 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 247 2170 2480 2626 3772 247
Size: 1923 310 146 1146 437
Fragments arranged by size:
1923 1146 437 310 146
EcoICRI GAG'CTC
Cuts at: 347 974 347
Size: 627 3335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 205 647 1787 2219 2966 3234 172
Size: 33 442 1140 432 747 268 900
Fragments arranged by size:
1140 900 747 442 432 268 33
EcoO109I rG'GnC_Cy
Cuts at: 246 278 842 1101 246
Size: 32 564 259 3107
Fragments arranged by size:
3107 564 259 32
EcoRI G'AATT_C
Cuts at: 966 966
Size: 3962
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1981 1981
Size: 3962
FspI TGC'GCA
Cuts at: 1743 1743
Size: 3962
HaeII r_GCGC'y
Cuts at: 1396 1644 2666 3645 1396
Size: 248 1022 979 1713
Fragments arranged by size:
1713 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 562 594 1804 1834 1836 1866 562
Size: 32 1210 30 2 30 2658
Fragments arranged by size:
2658 1210 32 30 30 2
HincII GTy'rAC
Cuts at: 1001 1001
Size: 3962
KasI G'GCGC_C
Cuts at: 1392 1640 1392
Size: 248 3714
KpnI G_GTAC'C
Cuts at: 982 982
Size: 3962
MluI A'CGCG_T
Cuts at: 3395 3395
Size: 3962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2633 2817 2633
Size: 184 3778
MscI TGG'CCA
Cuts at: 1723 1723
Size: 3962
MslI CAynn'nnrTG
Cuts at: 476 532 1951 2078 476
Size: 56 1419 127 2360
Fragments arranged by size:
2360 1419 127 56
MspA1I CmG'CkG
Cuts at: 456 1073 1387 1747 2760 3005 456
Size: 617 314 360 1013 245 1413
Fragments arranged by size:
1413 1013 617 360 314 245
NaeI GCC'GGC
Cuts at: 2143 2143
Size: 3962
NarI GG'CG_CC
Cuts at: 1393 1641 1393
Size: 248 3714
NcoI C'CATG_G
Cuts at: 107 560 107
Size: 453 3509
NgoMIV G'CCGG_C
Cuts at: 2141 2141
Size: 3962
NspI r_CATG'y
Cuts at: 428 576 1015 1515 2422 428
Size: 148 439 500 907 1968
Fragments arranged by size:
1968 907 500 439 148
PasI CC'CwG_GG
Cuts at: 249 249
Size: 3962
PciI A'CATG_T
Cuts at: 424 2418 424
Size: 1994 1968
PflMI CCAn_nnn'nTGG
Cuts at: 566 566
Size: 3962
PmeI GTTT'AAAC
Cuts at: 961 961
Size: 3962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3101 3133 3101
Size: 32 3930
PpuMI rG'GwC_Cy
Cuts at: 278 842 278
Size: 564 3398
PstI C_TGCA'G
Cuts at: 464 1009 1694 464
Size: 545 685 2732
Fragments arranged by size:
2732 685 545
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3962
PvuII CAG'CTG
Cuts at: 456 1387 1747 456
Size: 931 360 2671
Fragments arranged by size:
2671 931 360
RsrII CG'GwC_CG
Cuts at: 2157 2157
Size: 3962
SacI G_AGCT'C
Cuts at: 349 976 349
Size: 627 3335
SalI G'TCGA_C
Cuts at: 999 999
Size: 3962
SanDI GG'GwC_CC
Cuts at: 842 842
Size: 3962
SapI GCTCTTCn'nnn_
Cuts at: 148 1985 2195 148
Size: 1837 210 1915
Fragments arranged by size:
1915 1837 210
SbfI CC_TGCA'GG
Cuts at: 1009 1009
Size: 3962
ScaI AGT'ACT
Cuts at: 3479 3479
Size: 3962
SexAI A'CCwGG_T
Cuts at: 224 224
Size: 3962
SfcI C'TryA_G
Cuts at: 33 460 1005 1690 2683 2874 33
Size: 427 545 685 993 191 1121
Fragments arranged by size:
1121 993 685 545 427 191
SfoI GGC'GCC
Cuts at: 1394 1642 1394
Size: 248 3714
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
SmaI CCC'GGG
Cuts at: 847 984 847
Size: 137 3825
SmlI C'TyrA_G
Cuts at: 172 1117 2032 2524 2786 172
Size: 945 915 492 262 1348
Fragments arranged by size:
1348 945 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3962
SphI G_CATG'C
Cuts at: 1015 1515 1015
Size: 500 3462
StuI AGG'CCT
Cuts at: 598 598
Size: 3962
StyI C'CwwG_G
Cuts at: 107 560 1096 1450 107
Size: 453 536 354 2619
Fragments arranged by size:
2619 536 453 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1545 1678 2137 2346 1545
Size: 133 459 209 3161
Fragments arranged by size:
3161 459 209 133
TatI w'GTAC_w
Cuts at: 3477 3477
Size: 3962
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3479 3511 3479
Size: 32 3930
Tth111I GACn'n_nGTC
Cuts at: 1759 1759
Size: 3962
XbaI T'CTAG_A
Cuts at: 993 993
Size: 3962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1457 1457
Size: 3962
XmaI C'CCGG_G
Cuts at: 845 982 845
Size: 137 3825
ZraI GAC'GTC
Cuts at: 489 489
Size: 3962
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI