Restriction map for
pF1KE0100
(Circular) (Six-base) MAPSORT of: pF1KE0100.seq Check: 7283 from: 1 to: 5609
pF1KE0100 5609 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 821 2310 821
Size: 1489 4120
AccI GT'mk_AC
Cuts at: 999 1483 2647 999
Size: 484 1164 3961
Fragments arranged by size:
3961 1164 484
Acc65I G'GTAC_C
Cuts at: 2625 2625
Size: 5609
AclI AA'CG_TT
Cuts at: 1667 5449 1667
Size: 3782 1827
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2553 3434 3866 4219 4881 2553
Size: 881 432 353 662 3281
Fragments arranged by size:
3281 881 662 432 353
AfeI AGC'GCT
Cuts at: 2340 2340
Size: 5609
AflIII A'CryG_T
Cuts at: 2453 2961 4761 5042 2453
Size: 508 1800 281 3020
Fragments arranged by size:
3020 1800 508 281
AleI CACnn'nnGTG
Cuts at: 1021 1021
Size: 5609
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1657 1689 2361 2393 1657
Size: 32 672 32 4873
Fragments arranged by size:
4873 672 32 32
AlwNI CAG_nnn'CTG
Cuts at: 283 2914 4352 283
Size: 2631 1438 1540
Fragments arranged by size:
2631 1540 1438
ApaI G_GGCC'C
Cuts at: 173 173
Size: 5609
ApaLI G'TGCA_C
Cuts at: 4447 4447
Size: 5609
ApoI r'AATT_y
Cuts at: 802 1150 1591 2080 2147 2560 2613 4962 5218
Size: 348 441 489 67 413 53 2349 256
Cuts at: 5218 802
Size: 1193
Fragments arranged by size:
2349 1193 489 441 413 348 256 67
53
AseI AT'TA_AT
Cuts at: 20 1465 2593 20
Size: 1445 1128 3036
Fragments arranged by size:
3036 1445 1128
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5609
AvaI C'yCGr_G
Cuts at: 172 252 1436 2629 172
Size: 80 1184 1193 3152
Fragments arranged by size:
3152 1193 1184 80
BamHI G'GATC_C
Cuts at: 2066 2634 2066
Size: 568 5041
BanI G'GyrC_C
Cuts at: 256 891 2625 3039 3287 3322 256
Size: 635 1734 414 248 35 2543
Fragments arranged by size:
2543 1734 635 414 248 35
BanII G_rGCy'C
Cuts at: 173 226 253 2273 2623 3653 173
Size: 53 27 2020 350 1030 2129
Fragments arranged by size:
2129 2020 1030 350 53 27
BbeI G_GCGC'C
Cuts at: 260 3043 3291 260
Size: 2783 248 2578
Fragments arranged by size:
2783 2578 248
BbsI GAAGACnn'nnnn_
Cuts at: 912 1044 1815 912
Size: 132 771 4706
Fragments arranged by size:
4706 771 132
BciVI GTATCCnnnnn_n'
Cuts at: 931 1945 3507 4563 5609 931
Size: 1014 1562 1056 1046 931
Fragments arranged by size:
1562 1056 1046 1014 931
BclI T'GATC_A
Cuts at: 3129 3129
Size: 5609
BglI GCCn_nnn'nGGC
Cuts at: 409 1244 409
Size: 835 4774
BglII A'GATC_T
Cuts at: 3124 5161 3124
Size: 2037 3572
BlpI GC'TnA_GC
Cuts at: 2721 2721
Size: 5609
Bme1580I G_kGCm'C
Cuts at: 173 295 3234 3327 4451 173
Size: 122 2939 93 1124 1331
Fragments arranged by size:
2939 1331 1124 122 93
BmrI ACTGGGnnnn_n'
Cuts at: 2821 2902 3237 5381 2821
Size: 81 335 2144 3049
Fragments arranged by size:
3049 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3976 3976
Size: 5609
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 577 907 1438 2299 2363 2575 400
Size: 177 330 531 861 64 212 3434
Fragments arranged by size:
3434 861 531 330 212 177 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 708 1336 1390 1787 2287 2422 2785 3664 4378
Size: 628 54 397 500 135 363 879 714
Cuts at: 4378 4676 708
Size: 298 1641
Fragments arranged by size:
1641 879 714 628 500 397 363 298
135 54
BsaI GGTCTCn'nnnn_
Cuts at: 1513 2270 5313 1513
Size: 757 3043 1809
Fragments arranged by size:
3043 1809 757
BsaAI yAC'GTr
Cuts at: 1599 2454 2962 1599
Size: 855 508 4246
Fragments arranged by size:
4246 855 508
BsaBI GATnn'nnATC
Cuts at: 6 3123 5160 6
Size: 3117 2037 455
Fragments arranged by size:
3117 2037 455
BsaHI Gr'CG_yC
Cuts at: 257 3040 3288 3990 5486 257
Size: 2783 248 702 1496 380
Fragments arranged by size:
2783 1496 702 380 248
BsaWI w'CCGG_w
Cuts at: 2798 3319 3997 4408 4555 4829 2798
Size: 521 678 411 147 274 3578
Fragments arranged by size:
3578 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 550 580 1326 1356 1657 1687 2361 2391 550
Size: 30 746 30 301 30 674 30 3768
Fragments arranged by size:
3768 746 674 301 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 430 1435 430
Size: 1005 4604
BseYI C'CCAG_C
Cuts at: 1279 3034 3792 4457 4865 1279
Size: 1755 758 665 408 2023
Fragments arranged by size:
2023 1755 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 814
Size: 5609
BsiEI CG_ry'CG
Cuts at: 104 299 3151 3197 4427 104
Size: 195 2852 46 1230 1286
Fragments arranged by size:
2852 1286 1230 195 46
BsiHKAI G_wGCw'C
Cuts at: 2623 3401 3591 4451 2623
Size: 778 190 860 3781
Fragments arranged by size:
3781 860 778 190
BsmI GAATG_Cn'
Cuts at: 1697 2213 1697
Size: 516 5093
Bsp1286I G_dGCh'C
Cuts at: 173 226 253 295 2273 2623 3234 3327 3401
Size: 53 27 42 1978 350 611 93 74
Cuts at: 3401 3591 3653 4451 173
Size: 190 62 798 1331
Fragments arranged by size:
1978 1331 798 611 350 190 93 74
62 53 42 27
BspHI T'CATG_A
Cuts at: 4041 4041
Size: 5609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 488 821 2310 2659 2873 3175 3556 488
Size: 333 1489 349 214 302 381 2541
Fragments arranged by size:
2541 1489 381 349 333 302 214
BsrBI CCG'CTC
Cuts at: 50 361 3901 3955 5439 5604 50
Size: 311 3540 54 1484 165 55
Fragments arranged by size:
3540 1484 311 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1036 1511 3521 1036
Size: 475 2010 3124
Fragments arranged by size:
3124 2010 475
BsrFI r'CCGG_y
Cuts at: 3607 3788 3607
Size: 181 5428
BssSI C'ACGA_G
Cuts at: 3880 4588 3880
Size: 708 4901
BstAPI GCAn_nnn'nTGC
Cuts at: 283 2986 283
Size: 2703 2906
BstBI TT'CG_AA
Cuts at: 2151 3970 2151
Size: 1819 3790
BstXI CCAn_nnnn'nTGG
Cuts at: 378 2043 2553 378
Size: 1665 510 3434
Fragments arranged by size:
3434 1665 510
BstYI r'GATC_y
Cuts at: 1 1637 2066 2412 2634 3101 3124 3459 3705
Size: 1636 429 346 222 467 23 335 246
Cuts at: 3705 4011 4023 4109 5161 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1636 1052 467 449 429 346 335 306
246 222 86 23 12
BtgI C'CryG_G
Cuts at: 364 2062 2408 364
Size: 1698 346 3565
Fragments arranged by size:
3565 1698 346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3559 3740 3903 3559
Size: 181 163 5265
Fragments arranged by size:
5265 181 163
BtsI GCAGTG_nn'
Cuts at: 284 2852 284
Size: 2568 3041
DraI TTT'AAA
Cuts at: 2181 2608 2181
Size: 427 5182
DraIII CAC_nnn'GTG
Cuts at: 959 959
Size: 5609
DrdI GACnn_nn'nnGTC
Cuts at: 3315 4659 3315
Size: 1344 4265
EaeI y'GGCC_r
Cuts at: 296 1544 1761 3194 3368 3759 3786 5131 296
Size: 1248 217 1433 174 391 27 1345 774
Fragments arranged by size:
1433 1345 1248 774 391 217 174 27
EagI C'GGCC_G
Cuts at: 296 3194 296
Size: 2898 2711
EarI CTCTTCn'nnn_
Cuts at: 1230 1365 1565 2320 2359 3632 3842 1230
Size: 135 200 755 39 1273 210 2997
Fragments arranged by size:
2997 1273 755 210 200 135 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 194 390 3817 4559 4705 5419 194
Size: 196 3427 742 146 714 384
Fragments arranged by size:
3427 742 714 384 196 146
EcoICRI GAG'CTC
Cuts at: 2621 2621
Size: 5609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 577 907 1438 2299 2363 2553 2575 3434
Size: 177 330 531 861 64 190 22 859
Cuts at: 3434 3866 4219 4881 400
Size: 432 353 662 1128
Fragments arranged by size:
1128 861 859 662 531 432 353 330
190 177 64 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2317 2317
Size: 5609
EcoO109I rG'GnC_Cy
Cuts at: 2748 2748
Size: 5609
EcoRI G'AATT_C
Cuts at: 2613 2613
Size: 5609
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3628 3628
Size: 5609
FspI TGC'GCA
Cuts at: 3390 3390
Size: 5609
HaeII r_GCGC'y
Cuts at: 181 202 260 2342 3043 3291 4521 5292 181
Size: 21 58 2082 701 248 1230 771 498
Fragments arranged by size:
2082 1230 771 701 498 248 58 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 550 582 880 912 1201 1233 1326 1358 1392
Size: 32 298 32 289 32 93 32 34
Cuts at: 1392 1424 1465 1497 3451 3481 3483 3513 550
Size: 32 41 32 1954 30 2 30 2646
Fragments arranged by size:
2646 1954 298 289 93 41 34 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2648 2648
Size: 5609
KasI G'GCGC_C
Cuts at: 256 3039 3287 256
Size: 2783 248 2578
Fragments arranged by size:
2783 2578 248
KpnI G_GTAC'C
Cuts at: 2629 2629
Size: 5609
MfeI C'AATT_G
Cuts at: 1946 2499 1946
Size: 553 5056
MluI A'CGCG_T
Cuts at: 5042 5042
Size: 5609
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 496 1547 4368 4552 496
Size: 1051 2821 184 1553
Fragments arranged by size:
2821 1553 1051 184
MscI TGG'CCA
Cuts at: 3370 3370
Size: 5609
MslI CAynn'nnrTG
Cuts at: 1021 1060 2041 3598 3725 1021
Size: 39 981 1557 127 2905
Fragments arranged by size:
2905 1557 981 127 39
MspA1I CmG'CkG
Cuts at: 238 289 366 1683 2720 3034 3394 4178 4423
Size: 51 77 1317 1037 314 360 784 245
Cuts at: 4423 238
Size: 1424
Fragments arranged by size:
1424 1317 1037 784 360 314 245 77
51
NaeI GCC'GGC
Cuts at: 3790 3790
Size: 5609
NarI GG'CG_CC
Cuts at: 257 3040 3288 257
Size: 2783 248 2578
Fragments arranged by size:
2783 2578 248
NcoI C'CATG_G
Cuts at: 2408 2408
Size: 5609
NdeI CA'TA_TG
Cuts at: 1929 1929
Size: 5609
NgoMIV G'CCGG_C
Cuts at: 3788 3788
Size: 5609
NspI r_CATG'y
Cuts at: 2662 3162 4765 2662
Size: 500 1603 3506
Fragments arranged by size:
3506 1603 500
PacI TTA_AT'TAA
Cuts at: 1754 1754
Size: 5609
PciI A'CATG_T
Cuts at: 4761 4761
Size: 5609
PfoI T'CCnGG_A
Cuts at: 1076 2217 2379 2552 1076
Size: 1141 162 173 4133
Fragments arranged by size:
4133 1141 173 162
PmeI GTTT'AAAC
Cuts at: 2608 2608
Size: 5609
PmlI CAC'GTG
Cuts at: 1599 1599
Size: 5609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1657 1689 2361 2393 4055 4087 1657
Size: 32 672 32 1662 32 3179
Fragments arranged by size:
3179 1662 672 32 32 32
PsiI TTA'TAA
Cuts at: 2398 2398
Size: 5609
PspOMI G'GGCC_C
Cuts at: 169 169
Size: 5609
PstI C_TGCA'G
Cuts at: 288 485 1503 2656 3341 288
Size: 197 1018 1153 685 2556
Fragments arranged by size:
2556 1153 1018 685 197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5609
PvuII CAG'CTG
Cuts at: 1683 3034 3394 1683
Size: 1351 360 3898
Fragments arranged by size:
3898 1351 360
RsrII CG'GwC_CG
Cuts at: 265 3804 265
Size: 3539 2070
SacI G_AGCT'C
Cuts at: 2623 2623
Size: 5609
SacII CC_GC'GG
Cuts at: 367 367
Size: 5609
SalI G'TCGA_C
Cuts at: 2646 2646
Size: 5609
SapI GCTCTTCn'nnn_
Cuts at: 1565 2320 3632 3842 1565
Size: 755 1312 210 3332
Fragments arranged by size:
3332 1312 755 210
SbfI CC_TGCA'GG
Cuts at: 2656 2656
Size: 5609
ScaI AGT'ACT
Cuts at: 5126 5126
Size: 5609
SfcI C'TryA_G
Cuts at: 33 284 481 1499 1660 2204 2652 3337 4305
Size: 251 197 1018 161 544 448 685 968
Cuts at: 4305 4496 33
Size: 191 1146
Fragments arranged by size:
1146 1018 968 685 544 448 251 197
191 161
SfoI GGC'GCC
Cuts at: 258 3041 3289 258
Size: 2783 248 2578
Fragments arranged by size:
2783 2578 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5609
SmaI CCC'GGG
Cuts at: 174 2631 174
Size: 2457 3152
SmlI C'TyrA_G
Cuts at: 687 1315 1369 1802 2302 2437 2764 3679 4393
Size: 628 54 433 500 135 327 915 714
Cuts at: 4393 4655 687
Size: 262 1641
Fragments arranged by size:
1641 915 714 628 500 433 327 262
135 54
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5609
SphI G_CATG'C
Cuts at: 2662 3162 2662
Size: 500 5109
StyI C'CwwG_G
Cuts at: 2408 2743 3097 2408
Size: 335 354 4920
Fragments arranged by size:
4920 354 335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 474 1038 3192 3325 3784 3993 474
Size: 564 2154 133 459 209 2090
Fragments arranged by size:
2154 2090 564 459 209 133
TatI w'GTAC_w
Cuts at: 395 797 2510 5124 395
Size: 402 1713 2614 880
Fragments arranged by size:
2614 1713 880 402
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 704 736 2232 2264 5126 5158 704
Size: 32 1496 32 2862 32 1155
Fragments arranged by size:
2862 1496 1155 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3406 3406
Size: 5609
XbaI T'CTAG_A
Cuts at: 2640 2640
Size: 5609
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3104 3104
Size: 5609
XmaI C'CCGG_G
Cuts at: 172 2629 172
Size: 2457 3152
XmnI GAAnn'nnTTC
Cuts at: 1669 2213 2467 2563 1669
Size: 544 254 96 4715
Fragments arranged by size:
4715 544 254 96
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PacI PciI PfoI PmeI PmlI PpiI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI
BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI NheI NotI NruI
NsiI PasI PflMI PpuMI PshAI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI