Restriction map for
pF1KE0098
(Circular) (Six-base) MAPSORT of: pF1KE0098.seq Check: 8198 from: 1 to: 4211
pF1KE0098 4211 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 1249 1249
Size: 4211
Acc65I G'GTAC_C
Cuts at: 1227 1227
Size: 4211
AclI AA'CG_TT
Cuts at: 4051 4051
Size: 4211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 2036 2468 3215 3483 162
Size: 1874 432 747 268 890
Fragments arranged by size:
1874 890 747 432 268
AflIII A'CryG_T
Cuts at: 1563 2667 3644 1563
Size: 1104 977 2130
Fragments arranged by size:
2130 1104 977
AgeI A'CCGG_T
Cuts at: 2599 2599
Size: 4211
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 670 702 670
Size: 32 4179
AlwNI CAG_nnn'CTG
Cuts at: 138 1516 3083 138
Size: 1378 1567 1266
Fragments arranged by size:
1567 1378 1266
ApaI G_GGCC'C
Cuts at: 758 758
Size: 4211
ApaLI G'TGCA_C
Cuts at: 2981 2981
Size: 4211
ApoI r'AATT_y
Cuts at: 1215 3564 3820 1215
Size: 2349 256 1606
Fragments arranged by size:
2349 1606 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4211
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
AvaI C'yCGr_G
Cuts at: 151 751 792 1231 151
Size: 600 41 439 3131
Fragments arranged by size:
3131 600 439 41
BamHI G'GATC_C
Cuts at: 502 1236 502
Size: 734 3477
BanI G'GyrC_C
Cuts at: 899 961 1227 1641 1889 1924 899
Size: 62 266 414 248 35 3186
Fragments arranged by size:
3186 414 266 248 62 35
BanII G_rGCy'C
Cuts at: 752 758 1225 2255 752
Size: 6 467 1030 2708
Fragments arranged by size:
2708 1030 467 6
BbeI G_GCGC'C
Cuts at: 965 1645 1893 965
Size: 680 248 3283
Fragments arranged by size:
3283 680 248
BbvCI CC'TCA_GC
Cuts at: 314 314
Size: 4211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1058 1092 90
Size: 34 934 34 3209
Fragments arranged by size:
3209 934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2109 2870 4211 2109
Size: 761 1341 2109
Fragments arranged by size:
2109 1341 761
BclI T'GATC_A
Cuts at: 1731 1731
Size: 4211
BglII A'GATC_T
Cuts at: 839 1726 3763 839
Size: 887 2037 1287
Fragments arranged by size:
2037 1287 887
BlpI GC'TnA_GC
Cuts at: 1323 1323
Size: 4211
Bme1580I G_kGCm'C
Cuts at: 758 982 1836 1929 2985 758
Size: 224 854 93 1056 1984
Fragments arranged by size:
1984 1056 854 224 93
BmrI ACTGGGnnnn_n'
Cuts at: 496 1423 1504 1839 3983 496
Size: 927 81 335 2144 724
Fragments arranged by size:
2144 927 724 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 4211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 572 754 873 1085 1102 428
Size: 144 182 119 212 17 3537
Fragments arranged by size:
3537 212 182 144 119 17
Bpu10I CC'TnA_GC
Cuts at: 314 314
Size: 4211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 112 232 715 1387 2266 2758 3056 112
Size: 120 483 672 879 492 298 1267
Fragments arranged by size:
1267 879 672 492 483 298 120
BsaI GGTCTCn'nnnn_
Cuts at: 3915 3915
Size: 4211
BsaAI yAC'GTr
Cuts at: 1564 1564
Size: 4211
BsaBI GATnn'nnATC
Cuts at: 6 1725 3762 6
Size: 1719 2037 455
Fragments arranged by size:
2037 1719 455
BsaHI Gr'CG_yC
Cuts at: 395 962 1642 1890 2592 4088 395
Size: 567 680 248 702 1496 518
Fragments arranged by size:
1496 702 680 567 518 248
BsaWI w'CCGG_w
Cuts at: 546 1400 1921 2599 2873 3020 3431 546
Size: 854 521 678 274 147 411 1326
Fragments arranged by size:
1326 854 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1100 1130 1100
Size: 30 4181
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 640 643 640
Size: 3 4208
BseYI C'CCAG_C
Cuts at: 538 689 777 1033 1636 2394 2971 3467 538
Size: 151 88 256 603 758 577 496 1282
Fragments arranged by size:
1282 758 603 577 496 256 151 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 226
Size: 4211
BsiEI CG_ry'CG
Cuts at: 104 1753 1799 3007 104
Size: 1649 46 1208 1308
Fragments arranged by size:
1649 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 221 660 1225 2003 2193 2985 221
Size: 439 565 778 190 792 1447
Fragments arranged by size:
1447 792 778 565 439 190
BsiWI C'GTAC_G
Cuts at: 1202 1202
Size: 4211
BsmI GAATG_Cn'
Cuts at: 542 542
Size: 4211
BsmBI CGTCTCn'nnnn_
Cuts at: 899 899
Size: 4211
Bsp1286I G_dGCh'C
Cuts at: 221 660 752 758 982 1225 1836 1929 2003
Size: 439 92 6 224 243 611 93 74
Cuts at: 2003 2193 2255 2985 221
Size: 190 62 730 1447
Fragments arranged by size:
1447 730 611 439 243 224 190 93
92 74 62 6
BspEI T'CCGG_A
Cuts at: 546 3431 546
Size: 2885 1326
BspHI T'CATG_A
Cuts at: 3387 3387
Size: 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1261 1475 1777 2158 1261
Size: 214 302 381 3314
Fragments arranged by size:
3314 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2503 2557 4041 4206 50
Size: 2453 54 1484 165 55
Fragments arranged by size:
2453 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 852 2123 852
Size: 1271 2940
BsrFI r'CCGG_y
Cuts at: 893 2209 2390 2599 893
Size: 1316 181 209 2505
Fragments arranged by size:
2505 1316 209 181
BssSI C'ACGA_G
Cuts at: 2482 2840 2482
Size: 358 3853
BstAPI GCAn_nnn'nTGC
Cuts at: 138 1588 138
Size: 1450 2761
BstBI TT'CG_AA
Cuts at: 2572 2572
Size: 4211
BstXI CCAn_nnnn'nTGG
Cuts at: 232 1014 1041 232
Size: 782 27 3402
Fragments arranged by size:
3402 782 27
BstYI r'GATC_y
Cuts at: 1 134 411 502 839 1236 1703 1726 2061
Size: 133 277 91 337 397 467 23 335
Cuts at: 2061 2307 3319 3405 3417 3763 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 397 346 337 335 277
246 133 91 86 23 12
BtgI C'CryG_G
Cuts at: 194 473 194
Size: 279 3932
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2161 2342 2505 2161
Size: 181 163 3867
Fragments arranged by size:
3867 181 163
BtsI GCAGTG_nn'
Cuts at: 1061 1454 1061
Size: 393 3818
DraI TTT'AAA
Cuts at: 1210 1210
Size: 4211
DrdI GACnn_nn'nnGTC
Cuts at: 1917 2775 1917
Size: 858 3353
EaeI y'GGCC_r
Cuts at: 914 1796 1970 2361 2388 3733 914
Size: 882 174 391 27 1345 1392
Fragments arranged by size:
1392 1345 882 391 174 27
EagI C'GGCC_G
Cuts at: 1796 1796
Size: 4211
EarI CTCTTCn'nnn_
Cuts at: 181 617 1118 1198 2234 2444 181
Size: 436 501 80 1036 210 1948
Fragments arranged by size:
1948 1036 501 436 210 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2419 2729 2875 4021 2419
Size: 310 146 1146 2609
Fragments arranged by size:
2609 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1223 1223
Size: 4211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 428 572 754 873 1085 1102 2036 2468
Size: 266 144 182 119 212 17 934 432
Cuts at: 2468 3215 3483 162
Size: 747 268 890
Fragments arranged by size:
934 890 747 432 268 266 212 182
144 119 17
EcoO109I rG'GnC_Cy
Cuts at: 238 781 1350 238
Size: 543 569 3099
Fragments arranged by size:
3099 569 543
EcoRI G'AATT_C
Cuts at: 1215 1215
Size: 4211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 4211
FspI TGC'GCA
Cuts at: 1178 1992 1178
Size: 814 3397
HaeII r_GCGC'y
Cuts at: 965 1645 1893 2915 3894 965
Size: 680 248 1022 979 1282
Fragments arranged by size:
1282 1022 979 680 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 230 262 1115 1147 1175 1207 2053 2083 2085
Size: 32 853 32 28 32 846 30 2
Cuts at: 2085 2115 230
Size: 30 2326
Fragments arranged by size:
2326 853 846 32 32 32 30 30
28 2
HincII GTy'rAC
Cuts at: 858 1250 858
Size: 392 3819
HindIII A'AGCT_T
Cuts at: 764 764
Size: 4211
KasI G'GCGC_C
Cuts at: 961 1641 1889 961
Size: 680 248 3283
Fragments arranged by size:
3283 680 248
KpnI G_GTAC'C
Cuts at: 1231 1231
Size: 4211
MluI A'CGCG_T
Cuts at: 3644 3644
Size: 4211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 894 2882 3066 894
Size: 1988 184 2039
Fragments arranged by size:
2039 1988 184
MscI TGG'CCA
Cuts at: 1972 1972
Size: 4211
MslI CAynn'nnrTG
Cuts at: 2200 2327 2200
Size: 127 4084
MspA1I CmG'CkG
Cuts at: 1066 1083 1322 1636 1996 3009 3254 1066
Size: 17 239 314 360 1013 245 2023
Fragments arranged by size:
2023 1013 360 314 245 239 17
NaeI GCC'GGC
Cuts at: 895 2392 895
Size: 1497 2714
NarI GG'CG_CC
Cuts at: 962 1642 1890 962
Size: 680 248 3283
Fragments arranged by size:
3283 680 248
NcoI C'CATG_G
Cuts at: 194 473 194
Size: 279 3932
NgoMIV G'CCGG_C
Cuts at: 893 2390 893
Size: 1497 2714
NspI r_CATG'y
Cuts at: 1264 1764 2671 1264
Size: 500 907 2804
Fragments arranged by size:
2804 907 500
PciI A'CATG_T
Cuts at: 2667 2667
Size: 4211
PflMI CCAn_nnn'nTGG
Cuts at: 442 442
Size: 4211
PmeI GTTT'AAAC
Cuts at: 1210 1210
Size: 4211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 3350
Size: 32 4179
PpuMI rG'GwC_Cy
Cuts at: 238 238
Size: 4211
PspOMI G'GGCC_C
Cuts at: 754 754
Size: 4211
PspXI vC'TCGA_Gb
Cuts at: 151 151
Size: 4211
PstI C_TGCA'G
Cuts at: 1056 1258 1943 1056
Size: 202 685 3324
Fragments arranged by size:
3324 685 202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4211
PvuII CAG'CTG
Cuts at: 1636 1996 1636
Size: 360 3851
RsrII CG'GwC_CG
Cuts at: 2406 2406
Size: 4211
SacI G_AGCT'C
Cuts at: 1225 1225
Size: 4211
SalI G'TCGA_C
Cuts at: 1248 1248
Size: 4211
SanDI GG'GwC_CC
Cuts at: 238 238
Size: 4211
SapI GCTCTTCn'nnn_
Cuts at: 2234 2444 2234
Size: 210 4001
SbfI CC_TGCA'GG
Cuts at: 1258 1258
Size: 4211
ScaI AGT'ACT
Cuts at: 3728 3728
Size: 4211
SfcI C'TryA_G
Cuts at: 33 186 1052 1254 1939 2932 3123 33
Size: 153 866 202 685 993 191 1121
Fragments arranged by size:
1121 993 866 685 202 191 153
SfoI GGC'GCC
Cuts at: 963 1643 1891 963
Size: 680 248 3283
Fragments arranged by size:
3283 680 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
SmaI CCC'GGG
Cuts at: 794 1233 794
Size: 439 3772
SmlI C'TyrA_G
Cuts at: 127 151 247 694 1366 2281 2773 3035 127
Size: 24 96 447 672 915 492 262 1303
Fragments arranged by size:
1303 915 672 492 447 262 96 24
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4211
SphI G_CATG'C
Cuts at: 1264 1764 1264
Size: 500 3711
StyI C'CwwG_G
Cuts at: 168 194 473 569 995 1345 1699 168
Size: 26 279 96 426 350 354 2680
Fragments arranged by size:
2680 426 354 350 279 96 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 836 1794 1927 2386 2595 836
Size: 958 133 459 209 2452
Fragments arranged by size:
2452 958 459 209 133
TatI w'GTAC_w
Cuts at: 3726 3726
Size: 4211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1100 1132 3728 3760 1100
Size: 32 2596 32 1551
Fragments arranged by size:
2596 1551 32 32
Tth111I GACn'n_nGTC
Cuts at: 2008 2008
Size: 4211
XbaI T'CTAG_A
Cuts at: 1242 1242
Size: 4211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1706 1706
Size: 4211
XhoI C'TCGA_G
Cuts at: 151 151
Size: 4211
XmaI C'CCGG_G
Cuts at: 792 1231 792
Size: 439 3772
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI