Restriction map for
pF1KE0097
(Circular) (Six-base) MAPSORT of: pF1KE0097.seq Check: 7813 from: 1 to: 3962
pF1KE0097 3962 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 1000 1000
Size: 3962
Acc65I G'GTAC_C
Cuts at: 978 978
Size: 3962
AclI AA'CG_TT
Cuts at: 3802 3802
Size: 3962
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1787 2219 2966 3234 1787
Size: 432 747 268 2515
Fragments arranged by size:
2515 747 432 268
AflIII A'CryG_T
Cuts at: 373 617 714 1314 2418 3395 373
Size: 244 97 600 1104 977 940
Fragments arranged by size:
1104 977 940 600 244 97
AgeI A'CCGG_T
Cuts at: 2350 2350
Size: 3962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 758 773 758
Size: 15 3947
AlwNI CAG_nnn'CTG
Cuts at: 1267 2834 1267
Size: 1567 2395
ApaLI G'TGCA_C
Cuts at: 2732 2732
Size: 3962
ApoI r'AATT_y
Cuts at: 403 487 523 834 966 3315 3571 403
Size: 84 36 311 132 2349 256 794
Fragments arranged by size:
2349 794 311 256 132 84 36
AseI AT'TA_AT
Cuts at: 20 361 20
Size: 341 3621
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
AvaI C'yCGr_G
Cuts at: 982 982
Size: 3962
BamHI G'GATC_C
Cuts at: 987 987
Size: 3962
BanI G'GyrC_C
Cuts at: 978 1392 1640 1675 978
Size: 414 248 35 3265
Fragments arranged by size:
3265 414 248 35
BanII G_rGCy'C
Cuts at: 133 976 2006 133
Size: 843 1030 2089
Fragments arranged by size:
2089 1030 843
BbeI G_GCGC'C
Cuts at: 1396 1644 1396
Size: 248 3714
BbsI GAAGACnn'nnnn_
Cuts at: 620 620
Size: 3962
BciVI GTATCCnnnnn_n'
Cuts at: 692 1860 2621 3962 692
Size: 1168 761 1341 692
Fragments arranged by size:
1341 1168 761 692
BclI T'GATC_A
Cuts at: 1482 1482
Size: 3962
BglII A'GATC_T
Cuts at: 165 1477 3514 165
Size: 1312 2037 613
Fragments arranged by size:
2037 1312 613
BlpI GC'TnA_GC
Cuts at: 761 1074 761
Size: 313 3649
Bme1580I G_kGCm'C
Cuts at: 1587 1680 2736 1587
Size: 93 1056 2813
Fragments arranged by size:
2813 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1174 1255 1590 3734 1174
Size: 81 335 2144 1402
Fragments arranged by size:
2144 1402 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 3962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 571
Size: 3962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 2017 2509 2807 1138
Size: 879 492 298 2293
Fragments arranged by size:
2293 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3666 3666
Size: 3962
BsaAI yAC'GTr
Cuts at: 1315 1315
Size: 3962
BsaBI GATnn'nnATC
Cuts at: 6 1476 3513 6
Size: 1470 2037 455
Fragments arranged by size:
2037 1470 455
BsaHI Gr'CG_yC
Cuts at: 1393 1641 2343 3839 1393
Size: 248 702 1496 1516
Fragments arranged by size:
1516 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1151 1672 2350 2624 2771 3182 1151
Size: 521 678 274 147 411 1931
Fragments arranged by size:
1931 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 569 599 569
Size: 30 3932
BseYI C'CCAG_C
Cuts at: 1387 2145 2722 3218 1387
Size: 758 577 496 2131
Fragments arranged by size:
2131 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 616
Size: 3962
BsiEI CG_ry'CG
Cuts at: 104 1504 1550 2758 104
Size: 1400 46 1208 1308
Fragments arranged by size:
1400 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 133 976 1754 1944 2736 133
Size: 843 778 190 792 1359
Fragments arranged by size:
1359 843 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 133 976 1587 1680 1754 1944 2006 2736 133
Size: 843 611 93 74 190 62 730 1359
Fragments arranged by size:
1359 843 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3182 3182
Size: 3962
BspHI T'CATG_A
Cuts at: 692 3138 692
Size: 2446 1516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1012 1226 1528 1909 1012
Size: 214 302 381 3065
Fragments arranged by size:
3065 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2254 2308 3792 3957 50
Size: 2204 54 1484 165 55
Fragments arranged by size:
2204 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 151 757 1874 151
Size: 606 1117 2239
Fragments arranged by size:
2239 1117 606
BsrFI r'CCGG_y
Cuts at: 1960 2141 2350 1960
Size: 181 209 3572
Fragments arranged by size:
3572 209 181
BsrGI T'GTAC_A
Cuts at: 729 729
Size: 3962
BssSI C'ACGA_G
Cuts at: 2233 2591 2233
Size: 358 3604
BstAPI GCAn_nnn'nTGC
Cuts at: 1339 1339
Size: 3962
BstBI TT'CG_AA
Cuts at: 2323 2323
Size: 3962
BstYI r'GATC_y
Cuts at: 1 165 351 666 987 1454 1477 1812 2058
Size: 164 186 315 321 467 23 335 246
Cuts at: 2058 3070 3156 3168 3514 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 321 315 246
186 164 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3962
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1912 2093 2256 1912
Size: 181 163 3618
Fragments arranged by size:
3618 181 163
BtsI GCAGTG_nn'
Cuts at: 296 832 1205 296
Size: 536 373 3053
Fragments arranged by size:
3053 536 373
DraI TTT'AAA
Cuts at: 194 812 961 194
Size: 618 149 3195
Fragments arranged by size:
3195 618 149
DrdI GACnn_nn'nnGTC
Cuts at: 1668 2526 1668
Size: 858 3104
EaeI y'GGCC_r
Cuts at: 1547 1721 2112 2139 3484 1547
Size: 174 391 27 1345 2025
Fragments arranged by size:
2025 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1547 1547
Size: 3962
EarI CTCTTCn'nnn_
Cuts at: 1985 2195 1985
Size: 210 3752
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2170 2480 2626 3772 2170
Size: 310 146 1146 2360
Fragments arranged by size:
2360 1146 310 146
EcoICRI GAG'CTC
Cuts at: 131 974 131
Size: 843 3119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 1787 2219 2966 3234 571
Size: 1216 432 747 268 1299
Fragments arranged by size:
1299 1216 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1101 1101
Size: 3962
EcoRI G'AATT_C
Cuts at: 523 966 523
Size: 443 3519
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1981 1981
Size: 3962
FspI TGC'GCA
Cuts at: 1743 1743
Size: 3962
HaeII r_GCGC'y
Cuts at: 183 1396 1644 2666 3645 183
Size: 1213 248 1022 979 500
Fragments arranged by size:
1213 1022 979 500 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 549 569 581 601 1804 1834 1836 1866 549
Size: 20 12 20 1203 30 2 30 2645
Fragments arranged by size:
2645 1203 30 30 20 20 12 2
HincII GTy'rAC
Cuts at: 1001 1001
Size: 3962
KasI G'GCGC_C
Cuts at: 1392 1640 1392
Size: 248 3714
KpnI G_GTAC'C
Cuts at: 982 982
Size: 3962
MluI A'CGCG_T
Cuts at: 3395 3395
Size: 3962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2633 2817 2633
Size: 184 3778
MscI TGG'CCA
Cuts at: 1723 1723
Size: 3962
MslI CAynn'nnrTG
Cuts at: 1951 2078 1951
Size: 127 3835
MspA1I CmG'CkG
Cuts at: 1073 1387 1747 2760 3005 1073
Size: 314 360 1013 245 2030
Fragments arranged by size:
2030 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2143 2143
Size: 3962
NarI GG'CG_CC
Cuts at: 1393 1641 1393
Size: 248 3714
NcoI C'CATG_G
Cuts at: 107 107
Size: 3962
NdeI CA'TA_TG
Cuts at: 471 471
Size: 3962
NgoMIV G'CCGG_C
Cuts at: 2141 2141
Size: 3962
NspI r_CATG'y
Cuts at: 377 621 718 1015 1515 2422 377
Size: 244 97 297 500 907 1917
Fragments arranged by size:
1917 907 500 297 244 97
PciI A'CATG_T
Cuts at: 373 617 714 2418 373
Size: 244 97 1704 1917
Fragments arranged by size:
1917 1704 244 97
PflMI CCAn_nnn'nTGG
Cuts at: 752 752
Size: 3962
PmeI GTTT'AAAC
Cuts at: 961 961
Size: 3962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3101 3133 3101
Size: 32 3930
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 928 960 928
Size: 32 3930
PstI C_TGCA'G
Cuts at: 1009 1694 1009
Size: 685 3277
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3962
PvuII CAG'CTG
Cuts at: 1387 1747 1387
Size: 360 3602
RsrII CG'GwC_CG
Cuts at: 2157 2157
Size: 3962
SacI G_AGCT'C
Cuts at: 133 976 133
Size: 843 3119
SalI G'TCGA_C
Cuts at: 999 999
Size: 3962
SapI GCTCTTCn'nnn_
Cuts at: 1985 2195 1985
Size: 210 3752
SbfI CC_TGCA'GG
Cuts at: 1009 1009
Size: 3962
ScaI AGT'ACT
Cuts at: 3479 3479
Size: 3962
SfcI C'TryA_G
Cuts at: 33 657 945 1005 1690 2683 2874 33
Size: 624 288 60 685 993 191 1121
Fragments arranged by size:
1121 993 685 624 288 191 60
SfoI GGC'GCC
Cuts at: 1394 1642 1394
Size: 248 3714
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
SmaI CCC'GGG
Cuts at: 984 984
Size: 3962
SmlI C'TyrA_G
Cuts at: 1117 2032 2524 2786 1117
Size: 915 492 262 2293
Fragments arranged by size:
2293 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3962
SphI G_CATG'C
Cuts at: 1015 1515 1015
Size: 500 3462
SspI AAT'ATT
Cuts at: 217 233 217
Size: 16 3946
StyI C'CwwG_G
Cuts at: 107 1096 1450 107
Size: 989 354 2619
Fragments arranged by size:
2619 989 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1545 1678 2137 2346 1545
Size: 133 459 209 3161
Fragments arranged by size:
3161 459 209 133
TatI w'GTAC_w
Cuts at: 729 3477 729
Size: 2748 1214
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3479 3511 3479
Size: 32 3930
Tth111I GACn'n_nGTC
Cuts at: 1759 1759
Size: 3962
XbaI T'CTAG_A
Cuts at: 993 993
Size: 3962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1457 1457
Size: 3962
XmaI C'CCGG_G
Cuts at: 982 982
Size: 3962
XmnI GAAnn'nnTTC
Cuts at: 835 835
Size: 3962
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PmeI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI