Restriction map for
pF1KE0094
(Circular) (Six-base) MAPSORT of: pF1KE0094.seq Check: 394 from: 1 to: 4718
pF1KE0094 4718 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 863 1756 863
Size: 893 3825
Acc65I G'GTAC_C
Cuts at: 335 1734 335
Size: 1399 3319
AclI AA'CG_TT
Cuts at: 4558 4558
Size: 4718
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 1069 1195 1249 2543 2975 3722 3990 456
Size: 613 126 54 1294 432 747 268 1184
Fragments arranged by size:
1294 1184 747 613 432 268 126 54
AflIII A'CryG_T
Cuts at: 2070 3174 4151 2070
Size: 1104 977 2637
Fragments arranged by size:
2637 1104 977
AgeI A'CCGG_T
Cuts at: 1476 3106 1476
Size: 1630 3088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 223 682 223
Size: 459 4259
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 338 370 338
Size: 32 4686
AlwNI CAG_nnn'CTG
Cuts at: 792 960 1227 2023 3590 792
Size: 168 267 796 1567 1920
Fragments arranged by size:
1920 1567 796 267 168
ApaI G_GGCC'C
Cuts at: 813 813
Size: 4718
ApaLI G'TGCA_C
Cuts at: 3488 3488
Size: 4718
ApoI r'AATT_y
Cuts at: 1722 4071 4327 1722
Size: 2349 256 2113
Fragments arranged by size:
2349 2113 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4718
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4718
AvaI C'yCGr_G
Cuts at: 151 1738 151
Size: 1587 3131
BamHI G'GATC_C
Cuts at: 456 575 1743 456
Size: 119 1168 3431
Fragments arranged by size:
3431 1168 119
BanI G'GyrC_C
Cuts at: 335 1354 1734 2148 2396 2431 335
Size: 1019 380 414 248 35 2622
Fragments arranged by size:
2622 1019 414 380 248 35
BanII G_rGCy'C
Cuts at: 182 383 813 1732 2762 182
Size: 201 430 919 1030 2138
Fragments arranged by size:
2138 1030 919 430 201
BbeI G_GCGC'C
Cuts at: 2152 2400 2152
Size: 248 4470
BbsI GAAGACnn'nnnn_
Cuts at: 325 325
Size: 4718
BbvCI CC'TCA_GC
Cuts at: 926 926
Size: 4718
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1591 1625 1654 1688 1591
Size: 34 29 34 4621
Fragments arranged by size:
4621 34 34 29
BciVI GTATCCnnnnn_n'
Cuts at: 2616 3377 4718 2616
Size: 761 1341 2616
Fragments arranged by size:
2616 1341 761
BclI T'GATC_A
Cuts at: 2238 2238
Size: 4718
BglI GCCn_nnn'nGGC
Cuts at: 1015 1015
Size: 4718
BglII A'GATC_T
Cuts at: 1118 1148 1457 1556 2233 4270 1118
Size: 30 309 99 677 2037 1566
Fragments arranged by size:
2037 1566 677 309 99 30
BlpI GC'TnA_GC
Cuts at: 1830 1830
Size: 4718
Bme1580I G_kGCm'C
Cuts at: 525 813 1357 1652 2343 2436 3492 525
Size: 288 544 295 691 93 1056 1751
Fragments arranged by size:
1751 1056 691 544 295 288 93
BmrI ACTGGGnnnn_n'
Cuts at: 323 1930 2011 2346 4490 323
Size: 1607 81 335 2144 551
Fragments arranged by size:
2144 1607 551 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 261 1080 3085 261
Size: 819 2005 1894
Fragments arranged by size:
2005 1894 819
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 565 1264 1418 1663 277
Size: 288 699 154 245 3332
Fragments arranged by size:
3332 699 288 245 154
Bpu10I CC'TnA_GC
Cuts at: 377 926 377
Size: 549 4169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 652 712 1894 2773 3265 3563 166
Size: 486 60 1182 879 492 298 1321
Fragments arranged by size:
1321 1182 879 492 486 298 60
BsaI GGTCTCn'nnnn_
Cuts at: 4422 4422
Size: 4718
BsaAI yAC'GTr
Cuts at: 2071 2071
Size: 4718
BsaBI GATnn'nnATC
Cuts at: 6 2232 4269 6
Size: 2226 2037 455
Fragments arranged by size:
2226 2037 455
BsaHI Gr'CG_yC
Cuts at: 2149 2397 3099 4595 2149
Size: 248 702 1496 2272
Fragments arranged by size:
2272 1496 702 248
BsaWI w'CCGG_w
Cuts at: 908 1476 1907 2428 3106 3380 3527 3938 908
Size: 568 431 521 678 274 147 411 1688
Fragments arranged by size:
1688 678 568 521 431 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 856 886 856
Size: 30 4688
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 883 1321 883
Size: 438 4280
BseYI C'CCAG_C
Cuts at: 120 524 1280 1548 2143 2901 3478 3974 120
Size: 404 756 268 595 758 577 496 864
Fragments arranged by size:
864 758 756 595 577 496 404 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 1345
Size: 4718
BsiEI CG_ry'CG
Cuts at: 104 135 2260 2306 3514 104
Size: 31 2125 46 1208 1308
Fragments arranged by size:
2125 1308 1208 46 31
BsiHKAI G_wGCw'C
Cuts at: 1145 1732 2510 2700 3492 1145
Size: 587 778 190 792 2371
Fragments arranged by size:
2371 792 778 587 190
BsmBI CGTCTCn'nnnn_
Cuts at: 775 775
Size: 4718
Bsp1286I G_dGCh'C
Cuts at: 182 383 525 813 1145 1357 1652 1732 2343
Size: 201 142 288 332 212 295 80 611
Cuts at: 2343 2436 2510 2700 2762 3492 182
Size: 93 74 190 62 730 1408
Fragments arranged by size:
1408 730 611 332 295 288 212 201
190 142 93 80 74 62
BspEI T'CCGG_A
Cuts at: 3938 3938
Size: 4718
BspHI T'CATG_A
Cuts at: 3894 3894
Size: 4718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 347 1768 1982 2284 2665 347
Size: 1421 214 302 381 2400
Fragments arranged by size:
2400 1421 381 302 214
BsrBI CCG'CTC
Cuts at: 50 648 1137 3010 3064 4548 4713 50
Size: 598 489 1873 54 1484 165 55
Fragments arranged by size:
1873 1484 598 489 165 55 54
BsrDI GCAATG_nn'
Cuts at: 398 1274 2630 398
Size: 876 1356 2486
Fragments arranged by size:
2486 1356 876
BsrFI r'CCGG_y
Cuts at: 1476 2716 2897 3106 1476
Size: 1240 181 209 3088
Fragments arranged by size:
3088 1240 209 181
BssSI C'ACGA_G
Cuts at: 235 736 1651 2989 3347 235
Size: 501 915 1338 358 1606
Fragments arranged by size:
1606 1338 915 501 358
BstAPI GCAn_nnn'nTGC
Cuts at: 795 1656 2095 795
Size: 861 439 3418
Fragments arranged by size:
3418 861 439
BstBI TT'CG_AA
Cuts at: 3079 3079
Size: 4718
BstXI CCAn_nnnn'nTGG
Cuts at: 1329 1329
Size: 4718
BstYI r'GATC_y
Cuts at: 1 456 575 1118 1148 1457 1556 1743 2210
Size: 455 119 543 30 309 99 187 467
Cuts at: 2210 2233 2568 2814 3826 3912 3924 4270 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 543 467 455 449 346 335 309
246 187 119 99 86 30 23 12
BtgI C'CryG_G
Cuts at: 284 284
Size: 4718
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2668 2849 3012 2668
Size: 181 163 4374
Fragments arranged by size:
4374 181 163
BtsI GCAGTG_nn'
Cuts at: 1961 1961
Size: 4718
ClaI AT'CG_AT
Cuts at: 1409 1409
Size: 4718
DraI TTT'AAA
Cuts at: 1717 1717
Size: 4718
DrdI GACnn_nn'nnGTC
Cuts at: 2424 3282 2424
Size: 858 3860
EaeI y'GGCC_r
Cuts at: 1016 1112 1125 1430 1568 1616 2303 2477 2868
Size: 96 13 305 138 48 687 174 391
Cuts at: 2868 2895 4240 1016
Size: 27 1345 1494
Fragments arranged by size:
1494 1345 687 391 305 174 138 96
48 27 13
EagI C'GGCC_G
Cuts at: 2303 2303
Size: 4718
EarI CTCTTCn'nnn_
Cuts at: 212 676 1171 1225 2741 2951 212
Size: 464 495 54 1516 210 1979
Fragments arranged by size:
1979 1516 495 464 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1355 2926 3236 3382 4528 1355
Size: 1571 310 146 1146 1545
Fragments arranged by size:
1571 1545 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1730 1730
Size: 4718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 456 565 1069 1195 1249 1264 1418 1663
Size: 179 109 504 126 54 15 154 245
Cuts at: 1663 2543 2975 3722 3990 277
Size: 880 432 747 268 1005
Fragments arranged by size:
1005 880 747 504 432 268 245 179
154 126 109 54 15
EcoNI CCTnn'n_nnAGG
Cuts at: 330 330
Size: 4718
EcoO109I rG'GnC_Cy
Cuts at: 147 809 810 936 1857 147
Size: 662 1 126 921 3008
Fragments arranged by size:
3008 921 662 126 1
EcoRI G'AATT_C
Cuts at: 1722 1722
Size: 4718
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 342 486 2737 342
Size: 144 2251 2323
Fragments arranged by size:
2323 2251 144
FspI TGC'GCA
Cuts at: 982 2499 982
Size: 1517 3201
HaeII r_GCGC'y
Cuts at: 1502 2152 2400 3422 4401 1502
Size: 650 248 1022 979 1819
Fragments arranged by size:
1819 1022 979 650 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 691 723 1391 1423 2560 2590 2592 2622 691
Size: 32 668 32 1137 30 2 30 2787
Fragments arranged by size:
2787 1137 668 32 32 30 30 2
HincII GTy'rAC
Cuts at: 654 1104 1757 654
Size: 450 653 3615
Fragments arranged by size:
3615 653 450
KasI G'GCGC_C
Cuts at: 2148 2396 2148
Size: 248 4470
KpnI G_GTAC'C
Cuts at: 339 1738 339
Size: 1399 3319
MluI A'CGCG_T
Cuts at: 4151 4151
Size: 4718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3389 3573 3389
Size: 184 4534
MscI TGG'CCA
Cuts at: 1018 1432 1618 2479 1018
Size: 414 186 861 3257
Fragments arranged by size:
3257 861 414 186
MslI CAynn'nnrTG
Cuts at: 403 1327 1382 2707 2834 403
Size: 924 55 1325 127 2287
Fragments arranged by size:
2287 1325 924 127 55
MspA1I CmG'CkG
Cuts at: 116 625 792 1194 1227 1317 1829 2143 2503
Size: 509 167 402 33 90 512 314 360
Cuts at: 2503 3516 3761 116
Size: 1013 245 1073
Fragments arranged by size:
1073 1013 512 509 402 360 314 245
167 90 33
NaeI GCC'GGC
Cuts at: 2899 2899
Size: 4718
NarI GG'CG_CC
Cuts at: 2149 2397 2149
Size: 248 4470
NcoI C'CATG_G
Cuts at: 284 284
Size: 4718
NgoMIV G'CCGG_C
Cuts at: 2897 2897
Size: 4718
NspI r_CATG'y
Cuts at: 1263 1593 1771 2271 3178 1263
Size: 330 178 500 907 2803
Fragments arranged by size:
2803 907 500 330 178
PasI CC'CwG_GG
Cuts at: 436 436
Size: 4718
PciI A'CATG_T
Cuts at: 3174 3174
Size: 4718
PflMI CCAn_nnn'nTGG
Cuts at: 687 1287 687
Size: 600 4118
PmeI GTTT'AAAC
Cuts at: 1717 1717
Size: 4718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 3857
Size: 32 4686
PpuMI rG'GwC_Cy
Cuts at: 147 936 147
Size: 789 3929
PspOMI G'GGCC_C
Cuts at: 809 809
Size: 4718
PstI C_TGCA'G
Cuts at: 1199 1765 2450 1199
Size: 566 685 3467
Fragments arranged by size:
3467 685 566
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4718
PvuII CAG'CTG
Cuts at: 116 625 792 1194 2143 2503 116
Size: 509 167 402 949 360 2331
Fragments arranged by size:
2331 949 509 402 360 167
RsrII CG'GwC_CG
Cuts at: 2913 2913
Size: 4718
SacI G_AGCT'C
Cuts at: 1732 1732
Size: 4718
SalI G'TCGA_C
Cuts at: 1755 1755
Size: 4718
SapI GCTCTTCn'nnn_
Cuts at: 212 676 1225 2741 2951 212
Size: 464 549 1516 210 1979
Fragments arranged by size:
1979 1516 549 464 210
SbfI CC_TGCA'GG
Cuts at: 1765 1765
Size: 4718
ScaI AGT'ACT
Cuts at: 307 4235 307
Size: 3928 790
SfcI C'TryA_G
Cuts at: 33 1195 1761 2446 3439 3630 33
Size: 1162 566 685 993 191 1121
Fragments arranged by size:
1162 1121 993 685 566 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1015 1015
Size: 4718
SfoI GGC'GCC
Cuts at: 2150 2398 2150
Size: 248 4470
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4718
SgrAI Cr'CCGG_yG
Cuts at: 1476 1476
Size: 4718
SmaI CCC'GGG
Cuts at: 1740 1740
Size: 4718
SmlI C'TyrA_G
Cuts at: 181 631 727 1873 2788 3280 3542 181
Size: 450 96 1146 915 492 262 1357
Fragments arranged by size:
1357 1146 915 492 450 262 96
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4718
SphI G_CATG'C
Cuts at: 1593 1771 2271 1593
Size: 178 500 4040
Fragments arranged by size:
4040 500 178
StyI C'CwwG_G
Cuts at: 212 284 824 1019 1852 2206 212
Size: 72 540 195 833 354 2724
Fragments arranged by size:
2724 833 540 354 195 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1413 2301 2434 2893 3102 1413
Size: 888 133 459 209 3029
Fragments arranged by size:
3029 888 459 209 133
TatI w'GTAC_w
Cuts at: 305 4233 305
Size: 3928 790
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 560 592 4235 4267 560
Size: 32 3643 32 1011
Fragments arranged by size:
3643 1011 32 32
Tth111I GACn'n_nGTC
Cuts at: 2515 2515
Size: 4718
XbaI T'CTAG_A
Cuts at: 1749 1749
Size: 4718
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 291 310 1153 1515 1545 2213 291
Size: 19 843 362 30 668 2796
Fragments arranged by size:
2796 843 668 362 30 19
XmaI C'CCGG_G
Cuts at: 1738 1738
Size: 4718
XmnI GAAnn'nnTTC
Cuts at: 469 511 469
Size: 42 4676
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI