Restriction map for
pF1KE0090
(Circular) (Six-base) MAPSORT of: pF1KE0090.seq Check: 9368 from: 1 to: 4562
pF1KE0090 4562 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 1301 1600 1301
Size: 299 4263
Acc65I G'GTAC_C
Cuts at: 1578 1578
Size: 4562
AclI AA'CG_TT
Cuts at: 4402 4402
Size: 4562
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1424 2387 2819 3566 3834 1424
Size: 963 432 747 268 2152
Fragments arranged by size:
2152 963 747 432 268
AflIII A'CryG_T
Cuts at: 1914 3018 3995 1914
Size: 1104 977 2481
Fragments arranged by size:
2481 1104 977
AgeI A'CCGG_T
Cuts at: 2950 2950
Size: 4562
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1307 1307
Size: 4562
AlwNI CAG_nnn'CTG
Cuts at: 206 1867 3434 206
Size: 1661 1567 1334
Fragments arranged by size:
1661 1567 1334
ApaLI G'TGCA_C
Cuts at: 3332 3332
Size: 4562
ApoI r'AATT_y
Cuts at: 787 1097 1411 1566 3915 4171 787
Size: 310 314 155 2349 256 1178
Fragments arranged by size:
2349 1178 314 310 256 155
AseI AT'TA_AT
Cuts at: 20 20
Size: 4562
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4562
AvaI C'yCGr_G
Cuts at: 662 1582 662
Size: 920 3642
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 220 253 823 856 220
Size: 33 570 33 3926
Fragments arranged by size:
3926 570 33 33
BamHI G'GATC_C
Cuts at: 902 1587 902
Size: 685 3877
BanI G'GyrC_C
Cuts at: 1578 1992 2240 2275 1578
Size: 414 248 35 3865
Fragments arranged by size:
3865 414 248 35
BanII G_rGCy'C
Cuts at: 1043 1576 2606 1043
Size: 533 1030 2999
Fragments arranged by size:
2999 1030 533
BbeI G_GCGC'C
Cuts at: 1996 2244 1996
Size: 248 4314
BbsI GAAGACnn'nnnn_
Cuts at: 132 1327 132
Size: 1195 3367
BciVI GTATCCnnnnn_n'
Cuts at: 2460 3221 4562 2460
Size: 761 1341 2460
Fragments arranged by size:
2460 1341 761
BclI T'GATC_A
Cuts at: 389 2082 389
Size: 1693 2869
BfrBI ATG'CAT
Cuts at: 682 682
Size: 4562
BglI GCCn_nnn'nGGC
Cuts at: 547 547
Size: 4562
BglII A'GATC_T
Cuts at: 2077 4114 2077
Size: 2037 2525
BlpI GC'TnA_GC
Cuts at: 1674 1674
Size: 4562
Bme1580I G_kGCm'C
Cuts at: 1180 2187 2280 3336 1180
Size: 1007 93 1056 2406
Fragments arranged by size:
2406 1056 1007 93
BmrI ACTGGGnnnn_n'
Cuts at: 1774 1855 2190 4334 1774
Size: 81 335 2144 2002
Fragments arranged by size:
2144 2002 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2929 2929
Size: 4562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 573 713 984 1089 228
Size: 345 140 271 105 3701
Fragments arranged by size:
3701 345 271 140 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1738 2617 3109 3407 1738
Size: 879 492 298 2893
Fragments arranged by size:
2893 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4266 4266
Size: 4562
BsaAI yAC'GTr
Cuts at: 1278 1915 1278
Size: 637 3925
BsaBI GATnn'nnATC
Cuts at: 6 644 2076 4113 6
Size: 638 1432 2037 455
Fragments arranged by size:
2037 1432 638 455
BsaHI Gr'CG_yC
Cuts at: 1993 2241 2943 4439 1993
Size: 248 702 1496 2116
Fragments arranged by size:
2116 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1058 1751 2272 2950 3224 3371 3782 1058
Size: 693 521 678 274 147 411 1838
Fragments arranged by size:
1838 693 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 722 1232 722
Size: 510 4052
BseYI C'CCAG_C
Cuts at: 1029 1105 1130 1987 2745 3322 3818 1029
Size: 76 25 857 758 577 496 1773
Fragments arranged by size:
1773 857 758 577 496 76 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1300 1300
Size: 4562
BsiEI CG_ry'CG
Cuts at: 104 2104 2150 3358 104
Size: 2000 46 1208 1308
Fragments arranged by size:
2000 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 119 1043 1576 2354 2544 3336 119
Size: 924 533 778 190 792 1345
Fragments arranged by size:
1345 924 792 778 533 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1065 1065
Size: 4562
Bsp1286I G_dGCh'C
Cuts at: 119 1043 1180 1576 2187 2280 2354 2544 2606
Size: 924 137 396 611 93 74 190 62
Cuts at: 2606 3336 119
Size: 730 1345
Fragments arranged by size:
1345 924 730 611 396 190 137 93
74 62
BspEI T'CCGG_A
Cuts at: 3782 3782
Size: 4562
BspHI T'CATG_A
Cuts at: 783 3738 783
Size: 2955 1607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1068 1612 1826 2128 2509 1068
Size: 544 214 302 381 3121
Fragments arranged by size:
3121 544 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2854 2908 4392 4557 50
Size: 2804 54 1484 165 55
Fragments arranged by size:
2804 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2474 2474
Size: 4562
BsrFI r'CCGG_y
Cuts at: 2560 2741 2950 2560
Size: 181 209 4172
Fragments arranged by size:
4172 209 181
BssSI C'ACGA_G
Cuts at: 2833 3191 2833
Size: 358 4204
BstAPI GCAn_nnn'nTGC
Cuts at: 1939 1939
Size: 4562
BstBI TT'CG_AA
Cuts at: 2923 2923
Size: 4562
BstYI r'GATC_y
Cuts at: 1 902 1587 2054 2077 2412 2658 3670 3756
Size: 901 685 467 23 335 246 1012 86
Cuts at: 3756 3768 4114 1
Size: 12 346 449
Fragments arranged by size:
1012 901 685 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1306 1306
Size: 4562
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2512 2693 2856 2512
Size: 181 163 4218
Fragments arranged by size:
4218 181 163
BtsI GCAGTG_nn'
Cuts at: 167 536 1178 1805 167
Size: 369 642 627 2924
Fragments arranged by size:
2924 642 627 369
ClaI AT'CG_AT
Cuts at: 1436 1436
Size: 4562
DraI TTT'AAA
Cuts at: 1561 1561
Size: 4562
DrdI GACnn_nn'nnGTC
Cuts at: 2268 3126 2268
Size: 858 3704
EaeI y'GGCC_r
Cuts at: 422 792 1428 2147 2321 2712 2739 4084 422
Size: 370 636 719 174 391 27 1345 900
Fragments arranged by size:
1345 900 719 636 391 370 174 27
EagI C'GGCC_G
Cuts at: 2147 2147
Size: 4562
EarI CTCTTCn'nnn_
Cuts at: 442 1054 2585 2795 442
Size: 612 1531 210 2209
Fragments arranged by size:
2209 1531 612 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2770 3080 3226 4372 2770
Size: 310 146 1146 2960
Fragments arranged by size:
2960 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1041 1574 1041
Size: 533 4029
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 573 713 984 1089 1424 2387 2819 3566
Size: 345 140 271 105 335 963 432 747
Cuts at: 3566 3834 228
Size: 268 956
Fragments arranged by size:
963 956 747 432 345 335 271 268
140 105
EcoNI CCTnn'n_nnAGG
Cuts at: 198 229 695 198
Size: 31 466 4065
Fragments arranged by size:
4065 466 31
EcoO109I rG'GnC_Cy
Cuts at: 203 1198 1237 1701 203
Size: 995 39 464 3064
Fragments arranged by size:
3064 995 464 39
EcoRI G'AATT_C
Cuts at: 1097 1566 1097
Size: 469 4093
EcoRV GAT'ATC
Cuts at: 614 614
Size: 4562
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 4562
FspI TGC'GCA
Cuts at: 2343 2343
Size: 4562
HaeII r_GCGC'y
Cuts at: 563 1996 2244 3266 4245 563
Size: 1433 248 1022 979 880
Fragments arranged by size:
1433 1022 979 880 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 863 895 988 1020 2404 2434 2436 2466 863
Size: 32 93 32 1384 30 2 30 2959
Fragments arranged by size:
2959 1384 93 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1601 1601
Size: 4562
KasI G'GCGC_C
Cuts at: 1992 2240 1992
Size: 248 4314
KpnI G_GTAC'C
Cuts at: 1582 1582
Size: 4562
MluI A'CGCG_T
Cuts at: 3995 3995
Size: 4562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3233 3417 3233
Size: 184 4378
MscI TGG'CCA
Cuts at: 424 794 1430 2323 424
Size: 370 636 893 2663
Fragments arranged by size:
2663 893 636 370
MslI CAynn'nnrTG
Cuts at: 112 2551 2678 112
Size: 2439 127 1996
Fragments arranged by size:
2439 1996 127
MspA1I CmG'CkG
Cuts at: 1673 1987 2347 3360 3605 1673
Size: 314 360 1013 245 2630
Fragments arranged by size:
2630 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2743 2743
Size: 4562
NarI GG'CG_CC
Cuts at: 1993 2241 1993
Size: 248 4314
NgoMIV G'CCGG_C
Cuts at: 2741 2741
Size: 4562
NsiI A_TGCA'T
Cuts at: 684 684
Size: 4562
NspI r_CATG'y
Cuts at: 1615 2115 3022 1615
Size: 500 907 3155
Fragments arranged by size:
3155 907 500
PasI CC'CwG_GG
Cuts at: 801 801
Size: 4562
PciI A'CATG_T
Cuts at: 3018 3018
Size: 4562
PflMI CCAn_nnn'nTGG
Cuts at: 206 655 801 206
Size: 449 146 3967
Fragments arranged by size:
3967 449 146
PfoI T'CCnGG_A
Cuts at: 690 1543 690
Size: 853 3709
PmeI GTTT'AAAC
Cuts at: 1561 1561
Size: 4562
PmlI CAC'GTG
Cuts at: 1278 1278
Size: 4562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 3701
Size: 32 4530
PpuMI rG'GwC_Cy
Cuts at: 203 1198 1237 203
Size: 995 39 3528
Fragments arranged by size:
3528 995 39
PsiI TTA'TAA
Cuts at: 851 851
Size: 4562
PstI C_TGCA'G
Cuts at: 174 1609 2294 174
Size: 1435 685 2442
Fragments arranged by size:
2442 1435 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4562
PvuII CAG'CTG
Cuts at: 1987 2347 1987
Size: 360 4202
RsrII CG'GwC_CG
Cuts at: 2757 2757
Size: 4562
SacI G_AGCT'C
Cuts at: 1043 1576 1043
Size: 533 4029
SalI G'TCGA_C
Cuts at: 1599 1599
Size: 4562
SapI GCTCTTCn'nnn_
Cuts at: 1054 2585 2795 1054
Size: 1531 210 2821
Fragments arranged by size:
2821 1531 210
SbfI CC_TGCA'GG
Cuts at: 1609 1609
Size: 4562
ScaI AGT'ACT
Cuts at: 4079 4079
Size: 4562
SfcI C'TryA_G
Cuts at: 33 170 1350 1445 1605 2290 3283 3474 33
Size: 137 1180 95 160 685 993 191 1121
Fragments arranged by size:
1180 1121 993 685 191 160 137 95
SfiI GGCCn_nnn'nGGCC
Cuts at: 547 547
Size: 4562
SfoI GGC'GCC
Cuts at: 1994 2242 1994
Size: 248 4314
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4562
SmaI CCC'GGG
Cuts at: 1584 1584
Size: 4562
SmlI C'TyrA_G
Cuts at: 1717 2632 3124 3386 1717
Size: 915 492 262 2893
Fragments arranged by size:
2893 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4562
SphI G_CATG'C
Cuts at: 1615 2115 1615
Size: 500 4062
SspI AAT'ATT
Cuts at: 250 250
Size: 4562
StuI AGG'CCT
Cuts at: 550 701 550
Size: 151 4411
StyI C'CwwG_G
Cuts at: 1270 1696 2050 1270
Size: 426 354 3782
Fragments arranged by size:
3782 426 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2145 2278 2737 2946 2145
Size: 133 459 209 3761
Fragments arranged by size:
3761 459 209 133
TatI w'GTAC_w
Cuts at: 854 4077 854
Size: 3223 1339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 625 657 961 993 4079 4111 625
Size: 32 304 32 3086 32 1076
Fragments arranged by size:
3086 1076 304 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2359 2359
Size: 4562
XbaI T'CTAG_A
Cuts at: 1593 1593
Size: 4562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2057 2057
Size: 4562
XmaI C'CCGG_G
Cuts at: 1582 1582
Size: 4562
XmnI GAAnn'nnTTC
Cuts at: 303 931 303
Size: 628 3934
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BbvCI BcgI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I BtgI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SwaI
XhoI ZraI