Restriction map for
pF1KE0084
(Circular) (Six-base) MAPSORT of: pF1KE0084.seq Check: 9342 from: 1 to: 5729
pF1KE0084 5729 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2049 2049
Size: 5729
AatII G_ACGT'C
Cuts at: 965 965
Size: 5729
AccI GT'mk_AC
Cuts at: 944 2767 944
Size: 1823 3906
Acc65I G'GTAC_C
Cuts at: 2600 2745 2600
Size: 145 5584
AclI AA'CG_TT
Cuts at: 5569 5569
Size: 5729
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1323 1589 1672 1783 2129 2511 3554 3986
Size: 623 266 83 111 346 382 1043 432
Cuts at: 3986 4733 5001 700
Size: 747 268 1428
Fragments arranged by size:
1428 1043 747 623 432 382 346 268
266 111 83
AflIII A'CryG_T
Cuts at: 485 3081 4185 5162 485
Size: 2596 1104 977 1052
Fragments arranged by size:
2596 1104 1052 977
AgeI A'CCGG_T
Cuts at: 4117 4117
Size: 5729
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1775 1775
Size: 5729
AlwNI CAG_nnn'CTG
Cuts at: 810 1234 3034 4601 810
Size: 424 1800 1567 1938
Fragments arranged by size:
1938 1800 1567 424
ApaI G_GGCC'C
Cuts at: 2413 2413
Size: 5729
ApaLI G'TGCA_C
Cuts at: 4499 4499
Size: 5729
ApoI r'AATT_y
Cuts at: 1676 1705 2733 5082 5338 1676
Size: 29 1028 2349 256 2067
Fragments arranged by size:
2349 2067 1028 256 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 5729
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5729
AvaI C'yCGr_G
Cuts at: 277 561 2270 2375 2749 277
Size: 284 1709 105 374 3257
Fragments arranged by size:
3257 1709 374 284 105
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 894 927 894
Size: 33 5696
BamHI G'GATC_C
Cuts at: 2754 2754
Size: 5729
BanI G'GyrC_C
Cuts at: 243 841 2092 2600 2745 3159 3407 3442 243
Size: 598 1251 508 145 414 248 35 2530
Fragments arranged by size:
2530 1251 598 508 414 248 145 35
BanII G_rGCy'C
Cuts at: 116 368 862 1241 2413 2743 3773 116
Size: 252 494 379 1172 330 1030 2072
Fragments arranged by size:
2072 1172 1030 494 379 330 252
BbeI G_GCGC'C
Cuts at: 247 3163 3411 247
Size: 2916 248 2565
Fragments arranged by size:
2916 2565 248
BbsI GAAGACnn'nnnn_
Cuts at: 1607 1640 1955 1607
Size: 33 315 5381
Fragments arranged by size:
5381 315 33
BbvCI CC'TCA_GC
Cuts at: 2316 2316
Size: 5729
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2366 2400 2366
Size: 34 5695
BciVI GTATCCnnnnn_n'
Cuts at: 3627 4388 5729 3627
Size: 761 1341 3627
Fragments arranged by size:
3627 1341 761
BclI T'GATC_A
Cuts at: 1040 3249 1040
Size: 2209 3520
BglII A'GATC_T
Cuts at: 3244 5281 3244
Size: 2037 3692
BlpI GC'TnA_GC
Cuts at: 385 861 1235 2841 385
Size: 476 374 1606 3273
Fragments arranged by size:
3273 1606 476 374
Bme1580I G_kGCm'C
Cuts at: 876 2095 2413 3354 3447 4503 876
Size: 1219 318 941 93 1056 2102
Fragments arranged by size:
2102 1219 1056 941 318 93
BmgBI CAC'GTC
Cuts at: 255 255
Size: 5729
BmrI ACTGGGnnnn_n'
Cuts at: 388 2276 2941 3022 3357 5501 388
Size: 1888 665 81 335 2144 616
Fragments arranged by size:
2144 1888 665 616 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 576 4096 576
Size: 3520 2209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 472 604 1275 1375 2527 334
Size: 138 132 671 100 1152 3536
Fragments arranged by size:
3536 1152 671 138 132 100
Bpu10I CC'TnA_GC
Cuts at: 2316 2316
Size: 5729
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 100 1162 2905 3784 4276 4574 100
Size: 1062 1743 879 492 298 1255
Fragments arranged by size:
1743 1255 1062 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 450 1003 1387 2519 5433 450
Size: 553 384 1132 2914 746
Fragments arranged by size:
2914 1132 746 553 384
BsaAI yAC'GTr
Cuts at: 3082 3082
Size: 5729
BsaBI GATnn'nnATC
Cuts at: 6 3243 5280 6
Size: 3237 2037 455
Fragments arranged by size:
3237 2037 455
BsaHI Gr'CG_yC
Cuts at: 244 446 962 1127 3160 3408 4110 5606 244
Size: 202 516 165 2033 248 702 1496 367
Fragments arranged by size:
2033 1496 702 516 367 248 202 165
BsaWI w'CCGG_w
Cuts at: 830 2918 3439 4117 4391 4538 4949 830
Size: 2088 521 678 274 147 411 1610
Fragments arranged by size:
2088 1610 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2413 2443 2584 2614 2413
Size: 30 141 30 5528
Fragments arranged by size:
5528 141 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 1003 1015 2088 2297 2515 238
Size: 765 12 1073 209 218 3452
Fragments arranged by size:
3452 1073 765 218 209 12
BseYI C'CCAG_C
Cuts at: 189 467 1377 1892 2647 3154 3912 4489 4985
Size: 278 910 515 755 507 758 577 496
Cuts at: 4985 189
Size: 933
Fragments arranged by size:
933 910 758 755 577 515 507 496
278
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 2083 1204
Size: 879 4850
BsiEI CG_ry'CG
Cuts at: 104 215 327 3271 3317 4525 104
Size: 111 112 2944 46 1208 1308
Fragments arranged by size:
2944 1308 1208 112 111 46
BsiHKAI G_wGCw'C
Cuts at: 1241 2743 3521 3711 4503 1241
Size: 1502 778 190 792 2467
Fragments arranged by size:
2467 1502 792 778 190
BsmI GAATG_Cn'
Cuts at: 1876 1876
Size: 5729
BsmBI CGTCTCn'nnnn_
Cuts at: 1134 1329 1696 2436 2611 1134
Size: 195 367 740 175 4252
Fragments arranged by size:
4252 740 367 195 175
Bsp1286I G_dGCh'C
Cuts at: 116 368 862 876 1241 2095 2413 2743 3354
Size: 252 494 14 365 854 318 330 611
Cuts at: 3354 3447 3521 3711 3773 4503 116
Size: 93 74 190 62 730 1342
Fragments arranged by size:
1342 854 730 611 494 365 330 318
252 190 93 74 62 14
BspEI T'CCGG_A
Cuts at: 4949 4949
Size: 5729
BspHI T'CATG_A
Cuts at: 4905 4905
Size: 5729
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2049 2055 2273 2411 2779 2993 3295 3676 2049
Size: 6 218 138 368 214 302 381 4102
Fragments arranged by size:
4102 381 368 302 218 214 138 6
BsrBI CCG'CTC
Cuts at: 50 228 4021 4075 5559 5724 50
Size: 178 3793 54 1484 165 55
Fragments arranged by size:
3793 1484 178 165 55 54
BsrDI GCAATG_nn'
Cuts at: 771 3641 771
Size: 2870 2859
BsrFI r'CCGG_y
Cuts at: 214 419 3727 3908 4117 214
Size: 205 3308 181 209 1826
Fragments arranged by size:
3308 1826 209 205 181
BssHII G'CGCG_C
Cuts at: 121 121
Size: 5729
BssSI C'ACGA_G
Cuts at: 1412 2023 2497 4000 4358 1412
Size: 611 474 1503 358 2783
Fragments arranged by size:
2783 1503 611 474 358
BstAPI GCAn_nnn'nTGC
Cuts at: 810 3106 810
Size: 2296 3433
BstBI TT'CG_AA
Cuts at: 4090 4090
Size: 5729
BstEII G'GTnAC_C
Cuts at: 1585 2211 1585
Size: 626 5103
BstYI r'GATC_y
Cuts at: 1 629 806 2754 3221 3244 3579 3825 4837
Size: 628 177 1948 467 23 335 246 1012
Cuts at: 4837 4923 4935 5281 1
Size: 86 12 346 449
Fragments arranged by size:
1948 1012 628 467 449 346 335 246
177 86 23 12
BtgI C'CryG_G
Cuts at: 107 460 1592 2342 107
Size: 353 1132 750 3494
Fragments arranged by size:
3494 1132 750 353
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3679 3860 4023 3679
Size: 181 163 5385
Fragments arranged by size:
5385 181 163
BtsI GCAGTG_nn'
Cuts at: 2972 2972
Size: 5729
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2312 2347 2312
Size: 35 5694
DraI TTT'AAA
Cuts at: 2728 2728
Size: 5729
DraIII CAC_nnn'GTG
Cuts at: 2211 2211
Size: 5729
DrdI GACnn_nn'nnGTC
Cuts at: 3435 4293 3435
Size: 858 4871
EaeI y'GGCC_r
Cuts at: 212 324 371 773 1049 3314 3488 3879 3906
Size: 112 47 402 276 2265 174 391 27
Cuts at: 3906 5251 212
Size: 1345 690
Fragments arranged by size:
2265 1345 690 402 391 276 174 112
47 27
EagI C'GGCC_G
Cuts at: 212 324 3314 212
Size: 112 2990 2627
Fragments arranged by size:
2990 2627 112
EarI CTCTTCn'nnn_
Cuts at: 2369 3752 3962 2369
Size: 1383 210 4136
Fragments arranged by size:
4136 1383 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 182 1612 3937 4247 4393 5539 182
Size: 1430 2325 310 146 1146 372
Fragments arranged by size:
2325 1430 1146 372 310 146
EcoICRI GAG'CTC
Cuts at: 1239 2741 1239
Size: 1502 4227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 472 604 700 1275 1323 1375 1589 1672
Size: 138 132 96 575 48 52 214 83
Cuts at: 1672 1783 2129 2511 2527 3554 3986 4733 5001
Size: 111 346 382 16 1027 432 747 268
Cuts at: 5001 334
Size: 1062
Fragments arranged by size:
1062 1027 747 575 432 382 346 268
214 138 132 111 96 83 52 48
16
EcoNI CCTnn'n_nnAGG
Cuts at: 2608 2608
Size: 5729
EcoO109I rG'GnC_Cy
Cuts at: 302 347 1035 2179 2278 2409 2410 2868 302
Size: 45 688 1144 99 131 1 458 3163
Fragments arranged by size:
3163 1144 688 458 131 99 45 1
EcoRI G'AATT_C
Cuts at: 1676 2733 1676
Size: 1057 4672
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2034 3748 2034
Size: 1714 4015
FspI TGC'GCA
Cuts at: 814 3510 814
Size: 2696 3033
HaeII r_GCGC'y
Cuts at: 247 277 475 3163 3411 4433 5412 247
Size: 30 198 2688 248 1022 979 564
Fragments arranged by size:
2688 1022 979 564 248 198 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1348 1380 2413 2435 2445 2467 3571 3601 3603
Size: 32 1033 22 10 22 1104 30 2
Cuts at: 3603 3633 1348
Size: 30 3444
Fragments arranged by size:
3444 1104 1033 32 30 30 22 22
10 2
HincII GTy'rAC
Cuts at: 2493 2768 2493
Size: 275 5454
HindIII A'AGCT_T
Cuts at: 850 850
Size: 5729
KasI G'GCGC_C
Cuts at: 243 3159 3407 243
Size: 2916 248 2565
Fragments arranged by size:
2916 2565 248
KpnI G_GTAC'C
Cuts at: 2604 2749 2604
Size: 145 5584
MluI A'CGCG_T
Cuts at: 485 5162 485
Size: 4677 1052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 689 4400 4584 689
Size: 3711 184 1834
Fragments arranged by size:
3711 1834 184
MscI TGG'CCA
Cuts at: 373 775 1051 3490 373
Size: 402 276 2439 2612
Fragments arranged by size:
2612 2439 402 276
MslI CAynn'nnrTG
Cuts at: 718 3718 3845 718
Size: 3000 127 2602
Fragments arranged by size:
3000 2602 127
MspA1I CmG'CkG
Cuts at: 342 384 462 1150 1231 1353 2107 2651 2840
Size: 42 78 688 81 122 754 544 189
Cuts at: 2840 3154 3514 4527 4772 342
Size: 314 360 1013 245 1299
Fragments arranged by size:
1299 1013 754 688 544 360 314 245
189 122 81 78 42
NaeI GCC'GGC
Cuts at: 216 3910 216
Size: 3694 2035
NarI GG'CG_CC
Cuts at: 244 3160 3408 244
Size: 2916 248 2565
Fragments arranged by size:
2916 2565 248
NcoI C'CATG_G
Cuts at: 107 1592 107
Size: 1485 4244
NdeI CA'TA_TG
Cuts at: 1917 1917
Size: 5729
NgoMIV G'CCGG_C
Cuts at: 214 3908 214
Size: 3694 2035
NotI GC'GGCC_GC
Cuts at: 324 324
Size: 5729
NspI r_CATG'y
Cuts at: 708 819 1779 2040 2782 3282 4189 708
Size: 111 960 261 742 500 907 2248
Fragments arranged by size:
2248 960 907 742 500 261 111
PciI A'CATG_T
Cuts at: 4185 4185
Size: 5729
PflMI CCAn_nnn'nTGG
Cuts at: 489 1085 1377 489
Size: 596 292 4841
Fragments arranged by size:
4841 596 292
PfoI T'CCnGG_A
Cuts at: 1783 1783
Size: 5729
PmeI GTTT'AAAC
Cuts at: 2728 2728
Size: 5729
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4868 4900 4868
Size: 32 5697
PpuMI rG'GwC_Cy
Cuts at: 302 347 2278 302
Size: 45 1931 3753
Fragments arranged by size:
3753 1931 45
PspOMI G'GGCC_C
Cuts at: 2409 2409
Size: 5729
PstI C_TGCA'G
Cuts at: 806 2408 2776 3461 806
Size: 1602 368 685 3074
Fragments arranged by size:
3074 1602 685 368
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5729
PvuII CAG'CTG
Cuts at: 1231 1353 2107 3154 3514 1231
Size: 122 754 1047 360 3446
Fragments arranged by size:
3446 1047 754 360 122
RsrII CG'GwC_CG
Cuts at: 2421 3924 2421
Size: 1503 4226
SacI G_AGCT'C
Cuts at: 1241 2743 1241
Size: 1502 4227
SacII CC_GC'GG
Cuts at: 463 463
Size: 5729
SalI G'TCGA_C
Cuts at: 2766 2766
Size: 5729
SanDI GG'GwC_CC
Cuts at: 302 2278 302
Size: 1976 3753
SapI GCTCTTCn'nnn_
Cuts at: 3752 3962 3752
Size: 210 5519
SbfI CC_TGCA'GG
Cuts at: 2408 2776 2408
Size: 368 5361
ScaI AGT'ACT
Cuts at: 5246 5246
Size: 5729
SexAI A'CCwGG_T
Cuts at: 947 1289 2207 947
Size: 342 918 4469
Fragments arranged by size:
4469 918 342
SfcI C'TryA_G
Cuts at: 33 802 2196 2404 2772 3457 4450 4641 33
Size: 769 1394 208 368 685 993 191 1121
Fragments arranged by size:
1394 1121 993 769 685 368 208 191
SfoI GGC'GCC
Cuts at: 245 3161 3409 245
Size: 2916 248 2565
Fragments arranged by size:
2916 2565 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5729
SmaI CCC'GGG
Cuts at: 2751 2751
Size: 5729
SmlI C'TyrA_G
Cuts at: 115 1177 2884 3799 4291 4553 115
Size: 1062 1707 915 492 262 1291
Fragments arranged by size:
1707 1291 1062 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5729
SphI G_CATG'C
Cuts at: 819 2782 3282 819
Size: 1963 500 3266
Fragments arranged by size:
3266 1963 500
StuI AGG'CCT
Cuts at: 1725 1725
Size: 5729
StyI C'CwwG_G
Cuts at: 107 269 759 1592 2087 2863 3217 107
Size: 162 490 833 495 776 354 2619
Fragments arranged by size:
2619 833 776 495 490 354 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1092 2438 2478 3312 3445 3904 4113 1092
Size: 1346 40 834 133 459 209 2708
Fragments arranged by size:
2708 1346 834 459 209 133 40
TatI w'GTAC_w
Cuts at: 5244 5244
Size: 5729
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5246 5278 5246
Size: 32 5697
Tth111I GACn'n_nGTC
Cuts at: 2224 3526 2224
Size: 1302 4427
XbaI T'CTAG_A
Cuts at: 2760 2760
Size: 5729
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1374 3224 1374
Size: 1850 3879
XmaI C'CCGG_G
Cuts at: 2749 2749
Size: 5729
XmnI GAAnn'nnTTC
Cuts at: 1621 1621
Size: 5729
ZraI GAC'GTC
Cuts at: 963 963
Size: 5729
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI AscI AvrII BfrBI
BglI BmtI BsiWI BsrGI BstXI BstZ17I Bsu36I ClaI
EcoRV FseI FspAI HpaI MfeI NheI NruI NsiI
PacI PasI PmlI PshAI PsiI PspXI PsrI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI