Restriction map for
pF1KE0083
(Circular) (Six-base) MAPSORT of: pF1KE0083.seq Check: 6440 from: 1 to: 4688
pF1KE0083 4688 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 1726 1726
Size: 4688
Acc65I G'GTAC_C
Cuts at: 1704 1704
Size: 4688
AclI AA'CG_TT
Cuts at: 4528 4528
Size: 4688
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1154 2513 2945 3692 3960 880
Size: 274 1359 432 747 268 1608
Fragments arranged by size:
1608 1359 747 432 274 268
AflIII A'CryG_T
Cuts at: 1028 2040 3144 4121 1028
Size: 1012 1104 977 1595
Fragments arranged by size:
1595 1104 1012 977
AgeI A'CCGG_T
Cuts at: 3076 3076
Size: 4688
AleI CACnn'nnGTG
Cuts at: 1424 1424
Size: 4688
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 245 245
Size: 4688
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1472 1504 1472
Size: 32 4656
AlwNI CAG_nnn'CTG
Cuts at: 1593 1993 3560 1593
Size: 400 1567 2721
Fragments arranged by size:
2721 1567 400
ApaLI G'TGCA_C
Cuts at: 3458 3458
Size: 4688
ApoI r'AATT_y
Cuts at: 1628 1692 4041 4297 1628
Size: 64 2349 256 2019
Fragments arranged by size:
2349 2019 256 64
AseI AT'TA_AT
Cuts at: 20 493 20
Size: 473 4215
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
AvaI C'yCGr_G
Cuts at: 651 1708 651
Size: 1057 3631
AvrII C'CTAG_G
Cuts at: 606 606
Size: 4688
BamHI G'GATC_C
Cuts at: 1713 1713
Size: 4688
BanI G'GyrC_C
Cuts at: 1704 2118 2366 2401 1704
Size: 414 248 35 3991
Fragments arranged by size:
3991 414 248 35
BanII G_rGCy'C
Cuts at: 650 1702 2732 650
Size: 1052 1030 2606
Fragments arranged by size:
2606 1052 1030
BbeI G_GCGC'C
Cuts at: 2122 2370 2122
Size: 248 4440
BbsI GAAGACnn'nnnn_
Cuts at: 1604 1604
Size: 4688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1334 1368 1334
Size: 34 4654
BciVI GTATCCnnnnn_n'
Cuts at: 2586 3347 4688 2586
Size: 761 1341 2586
Fragments arranged by size:
2586 1341 761
BclI T'GATC_A
Cuts at: 414 2208 414
Size: 1794 2894
BglII A'GATC_T
Cuts at: 2203 4240 2203
Size: 2037 2651
BlpI GC'TnA_GC
Cuts at: 952 1800 952
Size: 848 3840
Bme1580I G_kGCm'C
Cuts at: 1582 2313 2406 3462 1582
Size: 731 93 1056 2808
Fragments arranged by size:
2808 1056 731 93
BmgBI CAC'GTC
Cuts at: 349 349
Size: 4688
BmrI ACTGGGnnnn_n'
Cuts at: 231 565 1900 1981 2316 4460 231
Size: 334 1335 81 335 2144 459
Fragments arranged by size:
2144 1335 459 335 334 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3055
Size: 4688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 1425 136
Size: 1289 3399
Bpu10I CC'TnA_GC
Cuts at: 1583 1583
Size: 4688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1864 2743 3235 3533 1864
Size: 879 492 298 3019
Fragments arranged by size:
3019 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4392 4392
Size: 4688
BsaAI yAC'GTr
Cuts at: 2041 2041
Size: 4688
BsaBI GATnn'nnATC
Cuts at: 6 1091 2202 4239 6
Size: 1085 1111 2037 455
Fragments arranged by size:
2037 1111 1085 455
BsaHI Gr'CG_yC
Cuts at: 2119 2367 3069 4565 2119
Size: 248 702 1496 2242
Fragments arranged by size:
2242 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1877 2398 3076 3350 3497 3908 1877
Size: 521 678 274 147 411 2657
Fragments arranged by size:
2657 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 443 709 1283 1380 1627 443
Size: 266 574 97 247 3504
Fragments arranged by size:
3504 574 266 247 97
BseYI C'CCAG_C
Cuts at: 2113 2871 3448 3944 2113
Size: 758 577 496 2857
Fragments arranged by size:
2857 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 981 1640 981
Size: 659 4029
BsiEI CG_ry'CG
Cuts at: 104 2230 2276 3484 104
Size: 2126 46 1208 1308
Fragments arranged by size:
2126 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 650 797 1702 2480 2670 3462 650
Size: 147 905 778 190 792 1876
Fragments arranged by size:
1876 905 792 778 190 147
BsmI GAATG_Cn'
Cuts at: 811 971 811
Size: 160 4528
Bsp1286I G_dGCh'C
Cuts at: 650 797 1582 1702 2313 2406 2480 2670 2732
Size: 147 785 120 611 93 74 190 62
Cuts at: 2732 3462 650
Size: 730 1876
Fragments arranged by size:
1876 785 730 611 190 147 120 93
74 62
BspEI T'CCGG_A
Cuts at: 3908 3908
Size: 4688
BspHI T'CATG_A
Cuts at: 3864 3864
Size: 4688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 947 1738 1952 2254 2635 947
Size: 791 214 302 381 3000
Fragments arranged by size:
3000 791 381 302 214
BsrBI CCG'CTC
Cuts at: 50 120 2980 3034 4518 4683 50
Size: 70 2860 54 1484 165 55
Fragments arranged by size:
2860 1484 165 70 55 54
BsrDI GCAATG_nn'
Cuts at: 270 2600 270
Size: 2330 2358
BsrFI r'CCGG_y
Cuts at: 2686 2867 3076 2686
Size: 181 209 4298
Fragments arranged by size:
4298 209 181
BssSI C'ACGA_G
Cuts at: 2959 3317 2959
Size: 358 4330
BstAPI GCAn_nnn'nTGC
Cuts at: 1031 2065 1031
Size: 1034 3654
BstBI TT'CG_AA
Cuts at: 255 924 963 3049 255
Size: 669 39 2086 1894
Fragments arranged by size:
2086 1894 669 39
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1426 114
Size: 1312 3376
BstYI r'GATC_y
Cuts at: 1 588 1713 2180 2203 2538 2784 3796 3882
Size: 587 1125 467 23 335 246 1012 86
Cuts at: 3882 3894 4240 1
Size: 12 346 449
Fragments arranged by size:
1125 1012 587 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 179 1475 107
Size: 72 1296 3320
Fragments arranged by size:
3320 1296 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2638 2819 2982 2638
Size: 181 163 4344
Fragments arranged by size:
4344 181 163
BtsI GCAGTG_nn'
Cuts at: 1931 1931
Size: 4688
DraI TTT'AAA
Cuts at: 620 1687 620
Size: 1067 3621
DrdI GACnn_nn'nnGTC
Cuts at: 2394 3252 2394
Size: 858 3830
EaeI y'GGCC_r
Cuts at: 884 2273 2447 2838 2865 4210 884
Size: 1389 174 391 27 1345 1362
Fragments arranged by size:
1389 1362 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2273 2273
Size: 4688
EarI CTCTTCn'nnn_
Cuts at: 536 2711 2921 536
Size: 2175 210 2303
Fragments arranged by size:
2303 2175 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2896 3206 3352 4498 2896
Size: 310 146 1146 3086
Fragments arranged by size:
3086 1146 310 146
EcoICRI GAG'CTC
Cuts at: 648 1700 648
Size: 1052 3636
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 880 1154 1425 2513 2945 3692 3960 136
Size: 744 274 271 1088 432 747 268 864
Fragments arranged by size:
1088 864 747 744 432 274 271 268
EcoO109I rG'GnC_Cy
Cuts at: 1590 1827 1590
Size: 237 4451
EcoRI G'AATT_C
Cuts at: 1692 1692
Size: 4688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 834 1025 2707 834
Size: 191 1682 2815
Fragments arranged by size:
2815 1682 191
FspI TGC'GCA
Cuts at: 2469 2469
Size: 4688
HaeII r_GCGC'y
Cuts at: 1389 2122 2370 3392 4371 1389
Size: 733 248 1022 979 1706
Fragments arranged by size:
1706 1022 979 733 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2530 2560 2562 2592 2530
Size: 30 2 30 4626
Fragments arranged by size:
4626 30 30 2
HincII GTy'rAC
Cuts at: 365 1727 365
Size: 1362 3326
KasI G'GCGC_C
Cuts at: 2118 2366 2118
Size: 248 4440
KpnI G_GTAC'C
Cuts at: 1708 1708
Size: 4688
MluI A'CGCG_T
Cuts at: 4121 4121
Size: 4688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1334 3359 3543 1334
Size: 2025 184 2479
Fragments arranged by size:
2479 2025 184
MscI TGG'CCA
Cuts at: 886 2449 886
Size: 1563 3125
MslI CAynn'nnrTG
Cuts at: 402 800 1424 2677 2804 402
Size: 398 624 1253 127 2286
Fragments arranged by size:
2286 1253 624 398 127
MspA1I CmG'CkG
Cuts at: 1406 1799 2113 2473 3486 3731 1406
Size: 393 314 360 1013 245 2363
Fragments arranged by size:
2363 1013 393 360 314 245
NaeI GCC'GGC
Cuts at: 2869 2869
Size: 4688
NarI GG'CG_CC
Cuts at: 2119 2367 2119
Size: 248 4440
NcoI C'CATG_G
Cuts at: 107 1475 107
Size: 1368 3320
NgoMIV G'CCGG_C
Cuts at: 2867 2867
Size: 4688
NspI r_CATG'y
Cuts at: 1032 1741 2241 3148 1032
Size: 709 500 907 2572
Fragments arranged by size:
2572 907 709 500
PciI A'CATG_T
Cuts at: 1028 3144 1028
Size: 2116 2572
PmeI GTTT'AAAC
Cuts at: 1687 1687
Size: 4688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 3827
Size: 32 4656
PstI C_TGCA'G
Cuts at: 1338 1735 2420 1338
Size: 397 685 3606
Fragments arranged by size:
3606 685 397
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4688
PvuII CAG'CTG
Cuts at: 2113 2473 2113
Size: 360 4328
RsrII CG'GwC_CG
Cuts at: 2883 2883
Size: 4688
SacI G_AGCT'C
Cuts at: 650 1702 650
Size: 1052 3636
SalI G'TCGA_C
Cuts at: 1725 1725
Size: 4688
SapI GCTCTTCn'nnn_
Cuts at: 536 2711 2921 536
Size: 2175 210 2303
Fragments arranged by size:
2303 2175 210
SbfI CC_TGCA'GG
Cuts at: 1735 1735
Size: 4688
ScaI AGT'ACT
Cuts at: 158 4205 158
Size: 4047 641
SfcI C'TryA_G
Cuts at: 33 1334 1731 2416 3409 3600 33
Size: 1301 397 685 993 191 1121
Fragments arranged by size:
1301 1121 993 685 397 191
SfoI GGC'GCC
Cuts at: 2120 2368 2120
Size: 248 4440
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
SmaI CCC'GGG
Cuts at: 1710 1710
Size: 4688
SmlI C'TyrA_G
Cuts at: 1843 2758 3250 3512 1843
Size: 915 492 262 3019
Fragments arranged by size:
3019 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4688
SphI G_CATG'C
Cuts at: 1741 2241 1741
Size: 500 4188
SspI AAT'ATT
Cuts at: 210 574 210
Size: 364 4324
StuI AGG'CCT
Cuts at: 1438 1438
Size: 4688
StyI C'CwwG_G
Cuts at: 107 606 1081 1475 1822 2176 107
Size: 499 475 394 347 354 2619
Fragments arranged by size:
2619 499 475 394 354 347
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 120 1616 2271 2404 2863 3072 120
Size: 1496 655 133 459 209 1736
Fragments arranged by size:
1736 1496 655 459 209 133
TatI w'GTAC_w
Cuts at: 156 1209 4203 156
Size: 1053 2994 641
Fragments arranged by size:
2994 1053 641
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 902 934 4205 4237 902
Size: 32 3271 32 1353
Fragments arranged by size:
3271 1353 32 32
Tth111I GACn'n_nGTC
Cuts at: 2485 2485
Size: 4688
XbaI T'CTAG_A
Cuts at: 1719 1719
Size: 4688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2183 2183
Size: 4688
XmaI C'CCGG_G
Cuts at: 1708 1708
Size: 4688
XmnI GAAnn'nnTTC
Cuts at: 843 843
Size: 4688
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI BaeI
BbvCI BfrBI BglI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI