Restriction map for
pF1KE0082
(Circular) (Six-base) MAPSORT of: pF1KE0082.seq Check: 7630 from: 1 to: 5099
pF1KE0082 5099 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 209 209
Size: 5099
AccI GT'mk_AC
Cuts at: 2137 2137
Size: 5099
Acc65I G'GTAC_C
Cuts at: 2115 2115
Size: 5099
AclI AA'CG_TT
Cuts at: 4939 4939
Size: 5099
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 855 961 1582 2924 3356 4103 4371 406
Size: 449 106 621 1342 432 747 268 1134
Fragments arranged by size:
1342 1134 747 621 449 432 268 106
AflIII A'CryG_T
Cuts at: 370 2451 3555 4532 370
Size: 2081 1104 977 937
Fragments arranged by size:
2081 1104 977 937
AgeI A'CCGG_T
Cuts at: 3487 3487
Size: 5099
AleI CACnn'nnGTG
Cuts at: 1670 1670
Size: 5099
AlwNI CAG_nnn'CTG
Cuts at: 2404 3971 2404
Size: 1567 3532
ApaLI G'TGCA_C
Cuts at: 1773 3869 1773
Size: 2096 3003
ApoI r'AATT_y
Cuts at: 176 905 1073 1201 1285 1453 1537 1661 1705
Size: 729 168 128 84 168 84 124 44
Cuts at: 1705 1803 1873 1997 2055 2103 4452 4708 176
Size: 98 70 124 58 48 2349 256 567
Fragments arranged by size:
2349 729 567 256 168 168 128 124
124 98 84 84 70 58 48 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 5099
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5099
AvaI C'yCGr_G
Cuts at: 2119 2119
Size: 5099
BamHI G'GATC_C
Cuts at: 2124 2124
Size: 5099
BanI G'GyrC_C
Cuts at: 279 1820 2115 2529 2777 2812 279
Size: 1541 295 414 248 35 2566
Fragments arranged by size:
2566 1541 414 295 248 35
BanII G_rGCy'C
Cuts at: 2113 3143 2113
Size: 1030 4069
BbeI G_GCGC'C
Cuts at: 2533 2781 2533
Size: 248 4851
BciVI GTATCCnnnnn_n'
Cuts at: 2997 3758 5099 2997
Size: 761 1341 2997
Fragments arranged by size:
2997 1341 761
BclI T'GATC_A
Cuts at: 2619 2619
Size: 5099
BfrBI ATG'CAT
Cuts at: 554 554
Size: 5099
BglII A'GATC_T
Cuts at: 588 2614 4651 588
Size: 2026 2037 1036
Fragments arranged by size:
2037 2026 1036
BlpI GC'TnA_GC
Cuts at: 407 2211 407
Size: 1804 3295
Bme1580I G_kGCm'C
Cuts at: 1777 2724 2817 3873 1777
Size: 947 93 1056 3003
Fragments arranged by size:
3003 1056 947 93
BmgBI CAC'GTC
Cuts at: 144 144
Size: 5099
BmrI ACTGGGnnnn_n'
Cuts at: 2311 2392 2727 4871 2311
Size: 81 335 2144 2539
Fragments arranged by size:
2539 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 496 3466 496
Size: 2970 2129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 851 935 1103 1187 1271 1355 1439 1523
Size: 610 84 168 84 84 84 84 84
Cuts at: 1523 1607 1943 1959 2027 241
Size: 84 336 16 68 3313
Fragments arranged by size:
3313 610 336 168 84 84 84 84
84 84 84 68 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2275 3154 3646 3944 2275
Size: 879 492 298 3430
Fragments arranged by size:
3430 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2007 4803 2007
Size: 2796 2303
BsaAI yAC'GTr
Cuts at: 2452 2452
Size: 5099
BsaBI GATnn'nnATC
Cuts at: 6 587 1652 2613 4650 6
Size: 581 1065 961 2037 455
Fragments arranged by size:
2037 1065 961 581 455
BsaHI Gr'CG_yC
Cuts at: 2530 2778 3480 4976 2530
Size: 248 702 1496 2653
Fragments arranged by size:
2653 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2288 2809 3487 3761 3908 4319 2288
Size: 521 678 274 147 411 3068
Fragments arranged by size:
3068 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 289 181
Size: 108 4991
BseYI C'CCAG_C
Cuts at: 356 2524 3282 3859 4355 356
Size: 2168 758 577 496 1100
Fragments arranged by size:
2168 1100 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 436 666 786 954 1203 1962 2046 187
Size: 249 230 120 168 249 759 84 3240
Fragments arranged by size:
3240 759 249 249 230 168 120 84
BsiEI CG_ry'CG
Cuts at: 104 2641 2687 3895 104
Size: 2537 46 1208 1308
Fragments arranged by size:
2537 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 731 1777 2113 2891 3081 3873 731
Size: 1046 336 778 190 792 1957
Fragments arranged by size:
1957 1046 792 778 336 190
BsmI GAATG_Cn'
Cuts at: 1273 1441 1525 1609 1273
Size: 168 84 84 4763
Fragments arranged by size:
4763 168 84 84
Bsp1286I G_dGCh'C
Cuts at: 731 1777 2113 2724 2817 2891 3081 3143 3873
Size: 1046 336 611 93 74 190 62 730
Cuts at: 3873 731
Size: 1957
Fragments arranged by size:
1957 1046 730 611 336 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4319 4319
Size: 5099
BspHI T'CATG_A
Cuts at: 1905 4275 1905
Size: 2370 2729
BspMI ACCTGCnnnn'nnnn_
Cuts at: 209 458 2149 2363 2665 3046 209
Size: 249 1691 214 302 381 2262
Fragments arranged by size:
2262 1691 381 302 249 214
BsrBI CCG'CTC
Cuts at: 50 3391 3445 4929 5094 50
Size: 3341 54 1484 165 55
Fragments arranged by size:
3341 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 298 1110 3011 298
Size: 812 1901 2386
Fragments arranged by size:
2386 1901 812
BsrFI r'CCGG_y
Cuts at: 3097 3278 3487 3097
Size: 181 209 4709
Fragments arranged by size:
4709 209 181
BsrGI T'GTAC_A
Cuts at: 1127 1555 1127
Size: 428 4671
BssSI C'ACGA_G
Cuts at: 3370 3728 3370
Size: 358 4741
BstAPI GCAn_nnn'nTGC
Cuts at: 2476 2476
Size: 5099
BstBI TT'CG_AA
Cuts at: 3460 3460
Size: 5099
BstEII G'GTnAC_C
Cuts at: 1063 1063
Size: 5099
BstYI r'GATC_y
Cuts at: 1 588 2124 2591 2614 2949 3195 4207 4293
Size: 587 1536 467 23 335 246 1012 86
Cuts at: 4293 4305 4651 1
Size: 12 346 449
Fragments arranged by size:
1536 1012 587 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 224 3049 3230 3393 224
Size: 2825 181 163 1930
Fragments arranged by size:
2825 1930 181 163
BtsI GCAGTG_nn'
Cuts at: 774 942 1530 1950 2034 2342 774
Size: 168 588 420 84 308 3531
Fragments arranged by size:
3531 588 420 308 168 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1092 1127 1260 1295 1428 1463 1092
Size: 35 133 35 133 35 4728
Fragments arranged by size:
4728 133 133 35 35 35
DraI TTT'AAA
Cuts at: 329 2098 329
Size: 1769 3330
DraIII CAC_nnn'GTG
Cuts at: 1781 1781
Size: 5099
DrdI GACnn_nn'nnGTC
Cuts at: 2805 3663 2805
Size: 858 4241
EaeI y'GGCC_r
Cuts at: 146 2684 2858 3249 3276 4621 146
Size: 2538 174 391 27 1345 624
Fragments arranged by size:
2538 1345 624 391 174 27
EagI C'GGCC_G
Cuts at: 2684 2684
Size: 5099
EarI CTCTTCn'nnn_
Cuts at: 611 1558 2034 3122 3332 611
Size: 947 476 1088 210 2378
Fragments arranged by size:
2378 1088 947 476 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3307 3617 3763 4909 3307
Size: 310 146 1146 3497
Fragments arranged by size:
3497 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2111 2111
Size: 5099
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 406 851 855 935 961 1103 1187 1271
Size: 165 445 4 80 26 142 84 84
Cuts at: 1271 1355 1439 1523 1582 1607 1943 1959 2027
Size: 84 84 84 59 25 336 16 68
Cuts at: 2027 2924 3356 4103 4371 241
Size: 897 432 747 268 969
Fragments arranged by size:
969 897 747 445 432 336 268 165
142 84 84 84 84 84 80 68
59 26 25 16 4
EcoNI CCTnn'n_nnAGG
Cuts at: 118 1045 118
Size: 927 4172
EcoO109I rG'GnC_Cy
Cuts at: 2238 2238
Size: 5099
EcoRI G'AATT_C
Cuts at: 176 1661 1997 2103 176
Size: 1485 336 106 3172
Fragments arranged by size:
3172 1485 336 106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 973 3118 973
Size: 2145 2954
FspI TGC'GCA
Cuts at: 2880 2880
Size: 5099
HaeII r_GCGC'y
Cuts at: 2533 2781 3803 4782 2533
Size: 248 1022 979 2850
Fragments arranged by size:
2850 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2004 2036 2941 2971 2973 3003 2004
Size: 32 905 30 2 30 4100
Fragments arranged by size:
4100 905 32 30 30 2
HincII GTy'rAC
Cuts at: 2138 2138
Size: 5099
HindIII A'AGCT_T
Cuts at: 442 950 442
Size: 508 4591
KasI G'GCGC_C
Cuts at: 2529 2777 2529
Size: 248 4851
KpnI G_GTAC'C
Cuts at: 2119 2119
Size: 5099
MluI A'CGCG_T
Cuts at: 4532 4532
Size: 5099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 824 3770 3954 824
Size: 2946 184 1969
Fragments arranged by size:
2946 1969 184
MscI TGG'CCA
Cuts at: 148 2860 148
Size: 2712 2387
MslI CAynn'nnrTG
Cuts at: 209 553 1670 3088 3215 209
Size: 344 1117 1418 127 2093
Fragments arranged by size:
2093 1418 1117 344 127
MspA1I CmG'CkG
Cuts at: 680 1217 1553 2210 2524 2884 3897 4142 680
Size: 537 336 657 314 360 1013 245 1637
Fragments arranged by size:
1637 1013 657 537 360 336 314 245
NaeI GCC'GGC
Cuts at: 3280 3280
Size: 5099
NarI GG'CG_CC
Cuts at: 2530 2778 2530
Size: 248 4851
NgoMIV G'CCGG_C
Cuts at: 3278 3278
Size: 5099
NsiI A_TGCA'T
Cuts at: 556 556
Size: 5099
NspI r_CATG'y
Cuts at: 374 554 558 2152 2652 3559 374
Size: 180 4 1594 500 907 1914
Fragments arranged by size:
1914 1594 907 500 180 4
PciI A'CATG_T
Cuts at: 370 3555 370
Size: 3185 1914
PmeI GTTT'AAAC
Cuts at: 2098 2098
Size: 5099
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4238 4270 4238
Size: 32 5067
PsiI TTA'TAA
Cuts at: 845 845
Size: 5099
PstI C_TGCA'G
Cuts at: 2146 2831 2146
Size: 685 4414
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5099
PvuII CAG'CTG
Cuts at: 680 1217 1553 2524 2884 680
Size: 537 336 971 360 2895
Fragments arranged by size:
2895 971 537 360 336
RsrII CG'GwC_CG
Cuts at: 3294 3294
Size: 5099
SacI G_AGCT'C
Cuts at: 2113 2113
Size: 5099
SalI G'TCGA_C
Cuts at: 2136 2136
Size: 5099
SapI GCTCTTCn'nnn_
Cuts at: 611 3122 3332 611
Size: 2511 210 2378
Fragments arranged by size:
2511 2378 210
SbfI CC_TGCA'GG
Cuts at: 2146 2146
Size: 5099
ScaI AGT'ACT
Cuts at: 4616 4616
Size: 5099
SfcI C'TryA_G
Cuts at: 33 302 2142 2827 3820 4011 33
Size: 269 1840 685 993 191 1121
Fragments arranged by size:
1840 1121 993 685 269 191
SfoI GGC'GCC
Cuts at: 2531 2779 2531
Size: 248 4851
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5099
SmaI CCC'GGG
Cuts at: 2121 2121
Size: 5099
SmlI C'TyrA_G
Cuts at: 2254 3169 3661 3923 2254
Size: 915 492 262 3430
Fragments arranged by size:
3430 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5099
SphI G_CATG'C
Cuts at: 558 2152 2652 558
Size: 1594 500 3005
Fragments arranged by size:
3005 1594 500
StuI AGG'CCT
Cuts at: 504 652 756 840 924 1008 1036 1176 1512
Size: 148 104 84 84 84 28 140 336
Cuts at: 1512 1680 504
Size: 168 3923
Fragments arranged by size:
3923 336 168 148 140 104 84 84
84 28
StyI C'CwwG_G
Cuts at: 266 1003 2233 2587 266
Size: 737 1230 354 2778
Fragments arranged by size:
2778 1230 737 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 361 605 700 2682 2815 3274 3483 361
Size: 244 95 1982 133 459 209 1977
Fragments arranged by size:
1982 1977 459 244 209 133 95
TatI w'GTAC_w
Cuts at: 1127 1555 4614 1127
Size: 428 3059 1612
Fragments arranged by size:
3059 1612 428
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4616 4648 4616
Size: 32 5067
Tth111I GACn'n_nGTC
Cuts at: 2896 2896
Size: 5099
XbaI T'CTAG_A
Cuts at: 2130 2130
Size: 5099
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2594 2594
Size: 5099
XmaI C'CCGG_G
Cuts at: 2119 2119
Size: 5099
XmnI GAAnn'nnTTC
Cuts at: 1036 1036
Size: 5099
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmtI Bpu10I
BsaXI BsiWI BsmBI BssHII BstXI BstZ17I Bsu36I BtgI
ClaI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI