Restriction map for
pF1KE0081
(Circular) (Six-base) MAPSORT of: pF1KE0081.seq Check: 2003 from: 1 to: 7259
pF1KE0081 7259 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 967 967
Size: 7259
AccI GT'mk_AC
Cuts at: 2483 4297 2483
Size: 1814 5445
Acc65I G'GTAC_C
Cuts at: 4275 4275
Size: 7259
AclI AA'CG_TT
Cuts at: 7099 7099
Size: 7259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 1344 2556 3182 5084 5516 6263 6531 539
Size: 805 1212 626 1902 432 747 268 1267
Fragments arranged by size:
1902 1267 1212 805 747 626 432 268
AflIII A'CryG_T
Cuts at: 2327 4611 5715 6692 2327
Size: 2284 1104 977 2894
Fragments arranged by size:
2894 2284 1104 977
AgeI A'CCGG_T
Cuts at: 901 5647 901
Size: 4746 2513
AhdI GACnn_n'nnGTC
Cuts at: 2088 3750 2088
Size: 1662 5597
AleI CACnn'nnGTG
Cuts at: 4120 4120
Size: 7259
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 306 338 306
Size: 32 7227
AlwNI CAG_nnn'CTG
Cuts at: 363 1260 2382 2579 2931 4564 6131 363
Size: 897 1122 197 352 1633 1567 1491
Fragments arranged by size:
1633 1567 1491 1122 897 352 197
ApaI G_GGCC'C
Cuts at: 1681 1681
Size: 7259
ApaLI G'TGCA_C
Cuts at: 6029 6029
Size: 7259
ApoI r'AATT_y
Cuts at: 1434 2197 4171 4263 6612 6868 1434
Size: 763 1974 92 2349 256 1825
Fragments arranged by size:
2349 1974 1825 763 256 92
AseI AT'TA_AT
Cuts at: 20 20
Size: 7259
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7259
AvaI C'yCGr_G
Cuts at: 643 1570 1648 1798 2610 4279 643
Size: 927 78 150 812 1669 3623
Fragments arranged by size:
3623 1669 927 812 150 78
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3430 3463 3430
Size: 33 7226
BamHI G'GATC_C
Cuts at: 4284 4284
Size: 7259
BanI G'GyrC_C
Cuts at: 132 4275 4689 4937 4972 132
Size: 4143 414 248 35 2419
Fragments arranged by size:
4143 2419 414 248 35
BanII G_rGCy'C
Cuts at: 1486 1681 1807 2611 2711 3139 3643 3689 4273
Size: 195 126 804 100 428 504 46 584
Cuts at: 4273 5303 1486
Size: 1030 3442
Fragments arranged by size:
3442 1030 804 584 504 428 195 126
100 46
BbeI G_GCGC'C
Cuts at: 4693 4941 4693
Size: 248 7011
BbsI GAAGACnn'nnnn_
Cuts at: 1211 2149 2164 2550 3170 1211
Size: 938 15 386 620 5300
Fragments arranged by size:
5300 938 620 386 15
BbvCI CC'TCA_GC
Cuts at: 574 574
Size: 7259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4077 4111 4077
Size: 34 7225
BciVI GTATCCnnnnn_n'
Cuts at: 1000 3431 5157 5918 7259 1000
Size: 2431 1726 761 1341 1000
Fragments arranged by size:
2431 1726 1341 1000 761
BclI T'GATC_A
Cuts at: 953 4779 953
Size: 3826 3433
BglI GCCn_nnn'nGGC
Cuts at: 4228 4228
Size: 7259
BglII A'GATC_T
Cuts at: 2066 3845 4774 6811 2066
Size: 1779 929 2037 2514
Fragments arranged by size:
2514 2037 1779 929
BlpI GC'TnA_GC
Cuts at: 1088 2587 4371 1088
Size: 1499 1784 3976
Fragments arranged by size:
3976 1784 1499
Bme1580I G_kGCm'C
Cuts at: 137 1590 1681 4884 4977 6033 137
Size: 1453 91 3203 93 1056 1363
Fragments arranged by size:
3203 1453 1363 1056 93 91
BmrI ACTGGGnnnn_n'
Cuts at: 138 4471 4552 4887 7031 138
Size: 4333 81 335 2144 366
Fragments arranged by size:
4333 2144 366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 736 1663 2625 5626 736
Size: 927 962 3001 2369
Fragments arranged by size:
3001 2369 962 927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 255 756 1459 1612 1618 1801 1903 2528
Size: 7 501 703 153 6 183 102 625
Cuts at: 2528 3097 3277 3760 248
Size: 569 180 483 3747
Fragments arranged by size:
3747 703 625 569 501 483 183 180
153 102 7 6
Bpu10I CC'TnA_GC
Cuts at: 574 3133 574
Size: 2559 4700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 516 2490 3758 4435 5314 5806 6104 330
Size: 186 1974 1268 677 879 492 298 1485
Fragments arranged by size:
1974 1485 1268 879 677 492 298 186
BsaI GGTCTCn'nnnn_
Cuts at: 1432 2526 3098 6963 1432
Size: 1094 572 3865 1728
Fragments arranged by size:
3865 1728 1094 572
BsaAI yAC'GTr
Cuts at: 2109 4612 2109
Size: 2503 4756
BsaBI GATnn'nnATC
Cuts at: 6 4773 6810 6
Size: 4767 2037 455
Fragments arranged by size:
4767 2037 455
BsaHI Gr'CG_yC
Cuts at: 2102 3370 4690 4938 5640 7136 2102
Size: 1268 1320 248 702 1496 2225
Fragments arranged by size:
2225 1496 1320 1268 702 248
BsaWI w'CCGG_w
Cuts at: 901 4448 4969 5647 5921 6068 6479 901
Size: 3547 521 678 274 147 411 1681
Fragments arranged by size:
3547 1681 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 444 474 1921 1951 444
Size: 30 1447 30 5752
Fragments arranged by size:
5752 1447 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 759 1552 1798 2143 2461 2464 2713 2736 759
Size: 793 246 345 318 3 249 23 5282
Fragments arranged by size:
5282 793 345 318 249 246 23 3
BseYI C'CCAG_C
Cuts at: 356 1516 1841 4684 5442 6019 6515 356
Size: 1160 325 2843 758 577 496 1100
Fragments arranged by size:
2843 1160 1100 758 577 496 325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1837 2776 4071 1837
Size: 939 1295 5025
Fragments arranged by size:
5025 1295 939
BsiEI CG_ry'CG
Cuts at: 104 2061 2165 4183 4801 4847 6055 104
Size: 1957 104 2018 618 46 1208 1308
Fragments arranged by size:
2018 1957 1308 1208 618 104 46
BsiHKAI G_wGCw'C
Cuts at: 1928 3139 4097 4273 5051 5241 6033 1928
Size: 1211 958 176 778 190 792 3154
Fragments arranged by size:
3154 1211 958 792 778 190 176
Bsp1286I G_dGCh'C
Cuts at: 137 1486 1590 1681 1807 1928 2611 2711 3139
Size: 1349 104 91 126 121 683 100 428
Cuts at: 3139 3643 3689 4097 4273 4884 4977 5051 5241
Size: 504 46 408 176 611 93 74 190
Cuts at: 5241 5303 6033 137
Size: 62 730 1363
Fragments arranged by size:
1363 1349 730 683 611 504 428 408
190 176 126 121 104 100 93 91
74 62 46
BspEI T'CCGG_A
Cuts at: 6479 6479
Size: 7259
BspHI T'CATG_A
Cuts at: 416 1064 3263 6435 416
Size: 648 2199 3172 1240
Fragments arranged by size:
3172 2199 1240 648
BspMI ACCTGCnnnn'nnnn_
Cuts at: 967 2081 3221 4309 4523 4825 5206 967
Size: 1114 1140 1088 214 302 381 3020
Fragments arranged by size:
3020 1140 1114 1088 381 302 214
BsrBI CCG'CTC
Cuts at: 50 414 1995 5551 5605 7089 7254 50
Size: 364 1581 3556 54 1484 165 55
Fragments arranged by size:
3556 1581 1484 364 165 55 54
BsrDI GCAATG_nn'
Cuts at: 629 2643 2663 3968 4100 4161 5171 629
Size: 2014 20 1305 132 61 1010 2717
Fragments arranged by size:
2717 2014 1305 1010 132 61 20
BsrFI r'CCGG_y
Cuts at: 901 2858 3089 5257 5438 5647 901
Size: 1957 231 2168 181 209 2513
Fragments arranged by size:
2513 2168 1957 231 209 181
BsrGI T'GTAC_A
Cuts at: 1169 1169
Size: 7259
BssSI C'ACGA_G
Cuts at: 154 2769 5530 5888 154
Size: 2615 2761 358 1525
Fragments arranged by size:
2761 2615 1525 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1021 4636 1021
Size: 3615 3644
BstBI TT'CG_AA
Cuts at: 5620 5620
Size: 7259
BstEII G'GTnAC_C
Cuts at: 2172 2172
Size: 7259
BstXI CCAn_nnnn'nTGG
Cuts at: 2394 2506 3015 3151 4035 2394
Size: 112 509 136 884 5618
Fragments arranged by size:
5618 884 509 136 112
BstYI r'GATC_y
Cuts at: 1 668 2066 3777 3816 3845 4284 4751 4774
Size: 667 1398 1711 39 29 439 467 23
Cuts at: 4774 5109 5355 6367 6453 6465 6811 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1711 1398 1012 667 467 449 439 346
335 246 86 39 29 23 12
Bsu36I CC'TnA_GG
Cuts at: 257 876 2748 257
Size: 619 1872 4768
Fragments arranged by size:
4768 1872 619
BtgI C'CryG_G
Cuts at: 286 708 2645 2783 3603 3689 286
Size: 422 1937 138 820 86 3856
Fragments arranged by size:
3856 1937 820 422 138 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 194 950 5209 5390 5553 194
Size: 756 4259 181 163 1900
Fragments arranged by size:
4259 1900 756 181 163
BtsI GCAGTG_nn'
Cuts at: 1010 1454 1929 3386 4502 1010
Size: 444 475 1457 1116 3767
Fragments arranged by size:
3767 1457 1116 475 444
ClaI AT'CG_AT
Cuts at: 4010 4010
Size: 7259
DraI TTT'AAA
Cuts at: 3336 4258 3336
Size: 922 6337
DraIII CAC_nnn'GTG
Cuts at: 3615 3615
Size: 7259
DrdI GACnn_nn'nnGTC
Cuts at: 493 4965 5823 493
Size: 4472 858 1929
Fragments arranged by size:
4472 1929 858
EaeI y'GGCC_r
Cuts at: 1295 2205 2384 3511 3606 4180 4229 4844 5018
Size: 910 179 1127 95 574 49 615 174
Cuts at: 5018 5409 5436 6781 1295
Size: 391 27 1345 1773
Fragments arranged by size:
1773 1345 1127 910 615 574 391 179
174 95 49 27
EagI C'GGCC_G
Cuts at: 4180 4844 4180
Size: 664 6595
EarI CTCTTCn'nnn_
Cuts at: 112 147 680 2393 2809 3676 5282 5492 112
Size: 35 533 1713 416 867 1606 210 1879
Fragments arranged by size:
1879 1713 1606 867 533 416 210 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3664 5467 5777 5923 7069 3664
Size: 1803 310 146 1146 3854
Fragments arranged by size:
3854 1803 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3137 4271 3137
Size: 1134 6125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 255 539 756 1344 1459 1612 1618 1801
Size: 7 284 217 588 115 153 6 183
Cuts at: 1801 1903 2528 2556 3097 3182 3277 3760 5084
Size: 102 625 28 541 85 95 483 1324
Cuts at: 5084 5516 6263 6531 248
Size: 432 747 268 976
Fragments arranged by size:
1324 976 747 625 588 541 483 432
284 268 217 183 153 115 102 95
85 28 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3861 3877 3861
Size: 16 7243
EcoO109I rG'GnC_Cy
Cuts at: 1950 1998 4398 1950
Size: 48 2400 4811
Fragments arranged by size:
4811 2400 48
EcoRI G'AATT_C
Cuts at: 1434 4263 1434
Size: 2829 4430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3672 5278 3672
Size: 1606 5653
FspI TGC'GCA
Cuts at: 963 3200 5040 963
Size: 2237 1840 3182
Fragments arranged by size:
3182 2237 1840
HaeII r_GCGC'y
Cuts at: 4693 4941 5963 6942 4693
Size: 248 1022 979 5010
Fragments arranged by size:
5010 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 872 904 2074 2106 2996 3028 3736 3768 5101
Size: 32 1170 32 890 32 708 32 1333
Cuts at: 5101 5131 5133 5163 872
Size: 30 2 30 2968
Fragments arranged by size:
2968 1333 1170 890 708 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3351 4298 3351
Size: 947 6312
HindIII A'AGCT_T
Cuts at: 426 551 426
Size: 125 7134
KasI G'GCGC_C
Cuts at: 4689 4937 4689
Size: 248 7011
KpnI G_GTAC'C
Cuts at: 4279 4279
Size: 7259
MluI A'CGCG_T
Cuts at: 6692 6692
Size: 7259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 757 2081 2201 2455 2579 5930 6114 757
Size: 1324 120 254 124 3351 184 1902
Fragments arranged by size:
3351 1902 1324 254 184 124 120
MscI TGG'CCA
Cuts at: 1297 2207 2386 3513 4231 5020 1297
Size: 910 179 1127 718 789 3536
Fragments arranged by size:
3536 1127 910 789 718 179
MslI CAynn'nnrTG
Cuts at: 1126 1885 2731 3013 3061 3149 3454 3914 4033
Size: 759 846 282 48 88 305 460 119
Cuts at: 4033 4120 5248 5375 1126
Size: 87 1128 127 3010
Fragments arranged by size:
3010 1128 846 759 460 305 282 127
119 88 87 48
MspA1I CmG'CkG
Cuts at: 277 817 1303 1862 2184 2506 2697 2822 2895
Size: 540 486 559 322 322 191 125 73
Cuts at: 2895 3366 3605 3960 4370 4684 5044 6057 6302
Size: 471 239 355 410 314 360 1013 245
Cuts at: 6302 277
Size: 1234
Fragments arranged by size:
1234 1013 559 540 486 471 410 360
355 322 322 314 245 239 191 125
73
NaeI GCC'GGC
Cuts at: 2860 5440 2860
Size: 2580 4679
NarI GG'CG_CC
Cuts at: 4690 4938 4690
Size: 248 7011
NcoI C'CATG_G
Cuts at: 708 2645 3689 708
Size: 1937 1044 4278
Fragments arranged by size:
4278 1937 1044
NdeI CA'TA_TG
Cuts at: 1899 1899
Size: 7259
NgoMIV G'CCGG_C
Cuts at: 2858 5438 2858
Size: 2580 4679
NspI r_CATG'y
Cuts at: 1368 1615 2331 4312 4812 5719 1368
Size: 247 716 1981 500 907 2908
Fragments arranged by size:
2908 1981 907 716 500 247
PasI CC'CwG_GG
Cuts at: 3504 3504
Size: 7259
PciI A'CATG_T
Cuts at: 2327 5715 2327
Size: 3388 3871
PflMI CCAn_nnn'nTGG
Cuts at: 363 714 3239 3520 3615 4143 363
Size: 351 2525 281 95 528 3479
Fragments arranged by size:
3479 2525 528 351 281 95
PfoI T'CCnGG_A
Cuts at: 671 1475 1778 671
Size: 804 303 6152
Fragments arranged by size:
6152 804 303
PmeI GTTT'AAAC
Cuts at: 4258 4258
Size: 7259
PmlI CAC'GTG
Cuts at: 2109 2109
Size: 7259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3403 3435 6398 6430 3403
Size: 32 2963 32 4232
Fragments arranged by size:
4232 2963 32 32
PpuMI rG'GwC_Cy
Cuts at: 1950 1950
Size: 7259
PshAI GACnn'nnGTC
Cuts at: 2164 2164
Size: 7259
PsiI TTA'TAA
Cuts at: 206 206
Size: 7259
PspOMI G'GGCC_C
Cuts at: 1677 1677
Size: 7259
PspXI vC'TCGA_Gb
Cuts at: 1798 1798
Size: 7259
PstI C_TGCA'G
Cuts at: 982 1449 2189 3365 3446 4306 4991 982
Size: 467 740 1176 81 860 685 3250
Fragments arranged by size:
3250 1176 860 740 685 467 81
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7259
PvuII CAG'CTG
Cuts at: 277 817 2184 2506 2697 2895 3366 3960 4684
Size: 540 1367 322 191 198 471 594 724
Cuts at: 4684 5044 277
Size: 360 2492
Fragments arranged by size:
2492 1367 724 594 540 471 360 322
198 191
RsrII CG'GwC_CG
Cuts at: 5454 5454
Size: 7259
SacI G_AGCT'C
Cuts at: 3139 4273 3139
Size: 1134 6125
SacII CC_GC'GG
Cuts at: 3606 3606
Size: 7259
SalI G'TCGA_C
Cuts at: 4296 4296
Size: 7259
SapI GCTCTTCn'nnn_
Cuts at: 680 2809 5282 5492 680
Size: 2129 2473 210 2447
Fragments arranged by size:
2473 2447 2129 210
SbfI CC_TGCA'GG
Cuts at: 4306 4306
Size: 7259
ScaI AGT'ACT
Cuts at: 950 2191 6776 950
Size: 1241 4585 1433
Fragments arranged by size:
4585 1433 1241
SexAI A'CCwGG_T
Cuts at: 1700 1700
Size: 7259
SfcI C'TryA_G
Cuts at: 33 978 1445 2185 3084 3361 3428 3442 4302
Size: 945 467 740 899 277 67 14 860
Cuts at: 4302 4987 5980 6171 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 945 899 860 740 685 467
277 191 67 14
SfoI GGC'GCC
Cuts at: 4691 4939 4691
Size: 248 7011
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7259
SgrAI Cr'CCGG_yG
Cuts at: 901 901
Size: 7259
SmaI CCC'GGG
Cuts at: 1572 4281 1572
Size: 2709 4550
SmlI C'TyrA_G
Cuts at: 309 531 1798 2469 3773 4414 5329 5821 6083
Size: 222 1267 671 1304 641 915 492 262
Cuts at: 6083 309
Size: 1485
Fragments arranged by size:
1485 1304 1267 915 671 641 492 262
222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7259
SphI G_CATG'C
Cuts at: 1368 1615 4312 4812 1368
Size: 247 2697 500 3815
Fragments arranged by size:
3815 2697 500 247
SspI AAT'ATT
Cuts at: 653 679 653
Size: 26 7233
StuI AGG'CCT
Cuts at: 1635 1849 3131 1635
Size: 214 1282 5763
Fragments arranged by size:
5763 1282 214
StyI C'CwwG_G
Cuts at: 708 1712 2645 2762 3281 3656 3689 3857 3878
Size: 1004 933 117 519 375 33 168 21
Cuts at: 3878 4393 4747 708
Size: 515 354 3220
Fragments arranged by size:
3220 1004 933 519 515 375 354 168
117 33 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 120 3585 4842 4975 5434 5643 120
Size: 3465 1257 133 459 209 1736
Fragments arranged by size:
3465 1736 1257 459 209 133
TatI w'GTAC_w
Cuts at: 639 948 1169 2189 3446 6774 639
Size: 309 221 1020 1257 3328 1124
Fragments arranged by size:
3328 1257 1124 1020 309 221
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 444 455 476 487 1921 1953 2223 2255 2405
Size: 11 21 11 1434 32 270 32 150
Cuts at: 2405 2437 3483 3515 6776 6808 444
Size: 32 1046 32 3261 32 895
Fragments arranged by size:
3261 1434 1046 895 270 150 32 32
32 32 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 5056 5056
Size: 7259
XbaI T'CTAG_A
Cuts at: 4290 4290
Size: 7259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1524 2652 3240 4754 1524
Size: 1128 588 1514 4029
Fragments arranged by size:
4029 1514 1128 588
XhoI C'TCGA_G
Cuts at: 1798 1798
Size: 7259
XmaI C'CCGG_G
Cuts at: 1570 4279 1570
Size: 2709 4550
XmnI GAAnn'nnTTC
Cuts at: 1468 2872 1468
Size: 1404 5855
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AscI AvrII BfrBI BmgBI
BmtI BsiWI BsmI BsmBI BssHII BstZ17I CspCI EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PsrI SanDI SfiI SnaBI SrfI SwaI ZraI