Restriction map for
pF1KE0078
(Circular) (Six-base) MAPSORT of: pF1KE0078.seq Check: 887 from: 1 to: 5669
pF1KE0078 5669 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 903 2454 2546 903
Size: 1551 92 4026
Fragments arranged by size:
4026 1551 92
AatII G_ACGT'C
Cuts at: 884 884
Size: 5669
AccI GT'mk_AC
Cuts at: 1286 2707 1286
Size: 1421 4248
Acc65I G'GTAC_C
Cuts at: 2201 2685 2201
Size: 484 5185
AclI AA'CG_TT
Cuts at: 5509 5509
Size: 5669
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 561 1315 1495 1576 3494 3926 4673 4941 561
Size: 754 180 81 1918 432 747 268 1289
Fragments arranged by size:
1918 1289 754 747 432 268 180 81
AfeI AGC'GCT
Cuts at: 1213 1213
Size: 5669
AflIII A'CryG_T
Cuts at: 1394 1571 3021 4125 5102 1394
Size: 177 1450 1104 977 1961
Fragments arranged by size:
1961 1450 1104 977 177
AgeI A'CCGG_T
Cuts at: 4057 4057
Size: 5669
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 640 1255 640
Size: 615 5054
AlwNI CAG_nnn'CTG
Cuts at: 140 774 1188 1317 1386 2974 4541 140
Size: 634 414 129 69 1588 1567 1268
Fragments arranged by size:
1588 1567 1268 634 414 129 69
ApaI G_GGCC'C
Cuts at: 118 171 539 1922 2144 2608 118
Size: 53 368 1383 222 464 3179
Fragments arranged by size:
3179 1383 464 368 222 53
ApaLI G'TGCA_C
Cuts at: 291 937 2193 2266 4439 291
Size: 646 1256 73 2173 1521
Fragments arranged by size:
2173 1521 1256 646 73
ApoI r'AATT_y
Cuts at: 2673 5022 5278 2673
Size: 2349 256 3064
Fragments arranged by size:
3064 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5669
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5669
AvaI C'yCGr_G
Cuts at: 379 635 1911 2584 2689 379
Size: 256 1276 673 105 3359
Fragments arranged by size:
3359 1276 673 256 105
BamHI G'GATC_C
Cuts at: 2694 2694
Size: 5669
BanI G'GyrC_C
Cuts at: 834 1090 2201 2494 2685 3099 3347 3382 834
Size: 256 1111 293 191 414 248 35 3121
Fragments arranged by size:
3121 1111 414 293 256 248 191 35
BanII G_rGCy'C
Cuts at: 118 147 171 539 554 1498 1922 2144 2576
Size: 29 24 368 15 944 424 222 432
Cuts at: 2576 2608 2683 3713 118
Size: 32 75 1030 2074
Fragments arranged by size:
2074 1030 944 432 424 368 222 75
32 29 24 15
BbeI G_GCGC'C
Cuts at: 2498 3103 3351 2498
Size: 605 248 4816
Fragments arranged by size:
4816 605 248
BbsI GAAGACnn'nnnn_
Cuts at: 1129 1129
Size: 5669
BbvCI CC'TCA_GC
Cuts at: 133 1963 133
Size: 1830 3839
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 798 832 90
Size: 34 674 34 4927
Fragments arranged by size:
4927 674 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3567 4328 5669 3567
Size: 761 1341 3567
Fragments arranged by size:
3567 1341 761
BclI T'GATC_A
Cuts at: 3189 3189
Size: 5669
BglI GCCn_nnn'nGGC
Cuts at: 250 770 1009 1622 2326 2372 2413 250
Size: 520 239 613 704 46 41 3506
Fragments arranged by size:
3506 704 613 520 239 46 41
BglII A'GATC_T
Cuts at: 2078 3184 5221 2078
Size: 1106 2037 2526
Fragments arranged by size:
2526 2037 1106
BlpI GC'TnA_GC
Cuts at: 628 1834 2781 628
Size: 1206 947 3516
Fragments arranged by size:
3516 1206 947
Bme1580I G_kGCm'C
Cuts at: 118 171 295 327 539 671 941 1066 1095
Size: 53 124 32 212 132 270 125 29
Cuts at: 1095 1505 1922 2144 2197 2270 2350 2391 2447
Size: 410 417 222 53 73 80 41 56
Cuts at: 2447 2608 3294 3387 4443 118
Size: 161 686 93 1056 1344
Fragments arranged by size:
1344 1056 686 417 410 270 222 212
161 132 125 124 93 80 73 56
53 53 41 32 29
BmgBI CAC'GTC
Cuts at: 1200 1518 1574 1200
Size: 318 56 5295
Fragments arranged by size:
5295 318 56
BmrI ACTGGGnnnn_n'
Cuts at: 738 1107 2881 2962 3297 5441 738
Size: 369 1774 81 335 2144 966
Fragments arranged by size:
2144 1774 966 369 335 81
BmtI G_CTAG'C
Cuts at: 617 617
Size: 5669
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4036 4036
Size: 5669
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1210 1408 1066
Size: 144 198 5327
Fragments arranged by size:
5327 198 144
Bpu10I CC'TnA_GC
Cuts at: 133 775 1963 2050 133
Size: 642 1188 87 3752
Fragments arranged by size:
3752 1188 642 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2845 3724 4216 4514 2845
Size: 879 492 298 4000
Fragments arranged by size:
4000 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 634 1787 2439 5373 634
Size: 1153 652 2934 930
Fragments arranged by size:
2934 1153 930 652
BsaAI yAC'GTr
Cuts at: 213 413 1119 1416 3022 213
Size: 200 706 297 1606 2860
Fragments arranged by size:
2860 1606 706 297 200
BsaBI GATnn'nnATC
Cuts at: 6 3183 5220 6
Size: 3177 2037 455
Fragments arranged by size:
3177 2037 455
BsaHI Gr'CG_yC
Cuts at: 881 2495 3100 3348 4050 5546 881
Size: 1614 605 248 702 1496 1004
Fragments arranged by size:
1614 1496 1004 702 605 248
BsaWI w'CCGG_w
Cuts at: 2858 3379 4057 4331 4478 4889 2858
Size: 521 678 274 147 411 3638
Fragments arranged by size:
3638 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1381 1411 1381
Size: 30 5639
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 262 294 364 1207 262
Size: 32 70 843 4724
Fragments arranged by size:
4724 843 70 32
BseYI C'CCAG_C
Cuts at: 111 216 277 442 670 864 1094 1845 1990
Size: 105 61 165 228 194 230 751 145
Cuts at: 1990 2012 2108 3094 3852 4429 4925 111
Size: 22 96 986 758 577 496 855
Fragments arranged by size:
986 855 758 751 577 496 230 228
194 165 145 105 96 61 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 703 871 895 931 1084 1303 1704 2482
Size: 567 168 24 36 153 219 401 778
Cuts at: 2482 2506 136
Size: 24 3299
Fragments arranged by size:
3299 778 567 401 219 168 153 36
24 24
BsiEI CG_ry'CG
Cuts at: 104 678 695 765 2075 3211 3257 4465 104
Size: 574 17 70 1310 1136 46 1208 1308
Fragments arranged by size:
1310 1308 1208 1136 574 70 46 17
BsiHKAI G_wGCw'C
Cuts at: 295 418 554 941 1606 2056 2197 2270 2683
Size: 123 136 387 665 450 141 73 413
Cuts at: 2683 3461 3651 4443 295
Size: 778 190 792 1521
Fragments arranged by size:
1521 792 778 665 450 413 387 190
141 136 123 73
Bsp1286I G_dGCh'C
Cuts at: 118 147 171 295 327 418 539 554 671
Size: 29 24 124 32 91 121 15 117
Cuts at: 671 941 1066 1095 1498 1505 1606 1922 2056
Size: 270 125 29 403 7 101 316 134
Cuts at: 2056 2144 2197 2270 2350 2391 2447 2576 2608
Size: 88 53 73 80 41 56 129 32
Cuts at: 2608 2683 3294 3387 3461 3651 3713 4443 118
Size: 75 611 93 74 190 62 730 1344
Fragments arranged by size:
1344 730 611 403 316 270 190 134
129 125 124 121 117 101 93 91
88 80 75 74 73 62 56 53
41 32 32 29 29 24 15 7
BspEI T'CCGG_A
Cuts at: 4889 4889
Size: 5669
BspHI T'CATG_A
Cuts at: 4845 4845
Size: 5669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 903 1514 1769 2454 2546 2561 2719 2933 3235
Size: 611 255 685 92 15 158 214 302
Cuts at: 3235 3616 903
Size: 381 2956
Fragments arranged by size:
2956 685 611 381 302 255 214 158
92 15
BsrBI CCG'CTC
Cuts at: 50 2037 3961 4015 5499 5664 50
Size: 1987 1924 54 1484 165 55
Fragments arranged by size:
1987 1924 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3581 3581
Size: 5669
BsrFI r'CCGG_y
Cuts at: 565 764 1694 1806 2117 3667 3848 4057 565
Size: 199 930 112 311 1550 181 209 2177
Fragments arranged by size:
2177 1550 930 311 209 199 181 112
BsrGI T'GTAC_A
Cuts at: 1397 1397
Size: 5669
BssSI C'ACGA_G
Cuts at: 823 1102 3940 4298 823
Size: 279 2838 358 2194
Fragments arranged by size:
2838 2194 358 279
BstAPI GCAn_nnn'nTGC
Cuts at: 918 1766 2247 3046 918
Size: 848 481 799 3541
Fragments arranged by size:
3541 848 799 481
BstBI TT'CG_AA
Cuts at: 4030 4030
Size: 5669
BstEII G'GTnAC_C
Cuts at: 561 561
Size: 5669
BstXI CCAn_nnnn'nTGG
Cuts at: 339 2178 339
Size: 1839 3830
BstYI r'GATC_y
Cuts at: 1 369 1559 2078 2694 3161 3184 3519 3765
Size: 368 1190 519 616 467 23 335 246
Cuts at: 3765 4777 4863 4875 5221 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1190 1012 616 519 467 449 368 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1344 1540 2473 1344
Size: 196 933 4540
Fragments arranged by size:
4540 933 196
BtgI C'CryG_G
Cuts at: 344 557 698 797 2252 2391 344
Size: 213 141 99 1455 139 3622
Fragments arranged by size:
3622 1455 213 141 139 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 465 801 1698 3619 3800 3963 465
Size: 336 897 1921 181 163 2171
Fragments arranged by size:
2171 1921 897 336 181 163
BtsI GCAGTG_nn'
Cuts at: 1228 1618 2268 2912 1228
Size: 390 650 644 3985
Fragments arranged by size:
3985 650 644 390
DraI TTT'AAA
Cuts at: 2668 2668
Size: 5669
DraIII CAC_nnn'GTG
Cuts at: 894 1518 894
Size: 624 5045
DrdI GACnn_nn'nnGTC
Cuts at: 3375 4233 3375
Size: 858 4811
EaeI y'GGCC_r
Cuts at: 329 341 431 458 692 734 762 794 1001
Size: 12 90 27 234 42 28 32 207
Cuts at: 1001 1139 1731 1983 2072 2168 2453 3254 3428
Size: 138 592 252 89 96 285 801 174
Cuts at: 3428 3819 3846 5191 329
Size: 391 27 1345 807
Fragments arranged by size:
1345 807 801 592 391 285 252 234
207 174 138 96 90 89 42 32
28 27 27 12
EagI C'GGCC_G
Cuts at: 692 762 2072 3254 692
Size: 70 1310 1182 3107
Fragments arranged by size:
3107 1310 1182 70
EarI CTCTTCn'nnn_
Cuts at: 961 1334 2065 3692 3902 961
Size: 373 731 1627 210 2728
Fragments arranged by size:
2728 1627 731 373 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1762 3877 4187 4333 5479 1762
Size: 2115 310 146 1146 1952
Fragments arranged by size:
2115 1952 1146 310 146
EcoICRI GAG'CTC
Cuts at: 552 2681 552
Size: 2129 3540
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 561 1066 1210 1315 1408 1495 1576 3494 3926
Size: 505 144 105 93 87 81 1918 432
Cuts at: 3926 4673 4941 561
Size: 747 268 1289
Fragments arranged by size:
1918 1289 747 505 432 268 144 105
93 87 81
EcoNI CCTnn'n_nnAGG
Cuts at: 238 460 889 238
Size: 222 429 5018
Fragments arranged by size:
5018 429 222
EcoO109I rG'GnC_Cy
Cuts at: 115 167 168 536 771 828 1314 1362 1918
Size: 52 1 368 235 57 486 48 556
Cuts at: 1918 1919 2140 2141 2604 2605 2808 115
Size: 1 221 1 463 1 203 2976
Fragments arranged by size:
2976 556 486 463 368 235 221 203
57 52 48 1 1 1 1
EcoRI G'AATT_C
Cuts at: 2673 2673
Size: 5669
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3688 3688
Size: 5669
FspI TGC'GCA
Cuts at: 3450 3450
Size: 5669
HaeII r_GCGC'y
Cuts at: 635 1215 1554 1846 2263 2498 3103 3351 4373
Size: 580 339 292 417 235 605 248 1022
Cuts at: 4373 5352 635
Size: 979 952
Fragments arranged by size:
1022 979 952 605 580 417 339 292
248 235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 400 432 1729 1761 3511 3541 3543 3573 400
Size: 32 1297 32 1750 30 2 30 2496
Fragments arranged by size:
2496 1750 1297 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2708 2708
Size: 5669
KasI G'GCGC_C
Cuts at: 2494 3099 3347 2494
Size: 605 248 4816
Fragments arranged by size:
4816 605 248
KpnI G_GTAC'C
Cuts at: 2205 2689 2205
Size: 484 5185
MluI A'CGCG_T
Cuts at: 5102 5102
Size: 5669
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 347 4340 4524 347
Size: 3993 184 1492
Fragments arranged by size:
3993 1492 184
MscI TGG'CCA
Cuts at: 331 433 460 1003 1141 1733 1985 2170 2455
Size: 102 27 543 138 592 252 185 285
Cuts at: 2455 3430 331
Size: 975 2570
Fragments arranged by size:
2570 975 592 543 285 252 185 138
102 27
MslI CAynn'nnrTG
Cuts at: 337 2176 3658 3785 337
Size: 1839 1482 127 2221
Fragments arranged by size:
2221 1839 1482 127
MspA1I CmG'CkG
Cuts at: 687 700 799 840 1098 1294 1431 1448 1565
Size: 13 99 41 258 196 137 17 117
Cuts at: 1565 2780 3094 3454 4467 4712 687
Size: 1215 314 360 1013 245 1644
Fragments arranged by size:
1644 1215 1013 360 314 258 245 196
137 117 99 41 17 13
NaeI GCC'GGC
Cuts at: 766 1808 3850 766
Size: 1042 2042 2585
Fragments arranged by size:
2585 2042 1042
NarI GG'CG_CC
Cuts at: 2495 3100 3348 2495
Size: 605 248 4816
Fragments arranged by size:
4816 605 248
NcoI C'CATG_G
Cuts at: 557 557
Size: 5669
NgoMIV G'CCGG_C
Cuts at: 764 1806 3848 764
Size: 1042 2042 2585
Fragments arranged by size:
2585 2042 1042
NheI G'CTAG_C
Cuts at: 613 613
Size: 5669
NspI r_CATG'y
Cuts at: 1398 2247 2722 3222 4129 1398
Size: 849 475 500 907 2938
Fragments arranged by size:
2938 907 849 500 475
PasI CC'CwG_GG
Cuts at: 157 234 741 1167 2144 2608 157
Size: 77 507 426 977 464 3218
Fragments arranged by size:
3218 977 507 464 426 77
PciI A'CATG_T
Cuts at: 1394 4125 1394
Size: 2731 2938
PflMI CCAn_nnn'nTGG
Cuts at: 995 1518 995
Size: 523 5146
PfoI T'CCnGG_A
Cuts at: 1385 1385
Size: 5669
PmeI GTTT'AAAC
Cuts at: 2668 2668
Size: 5669
PmlI CAC'GTG
Cuts at: 1416 1416
Size: 5669
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4808 4840 4808
Size: 32 5637
PpuMI rG'GwC_Cy
Cuts at: 1362 1362
Size: 5669
PspOMI G'GGCC_C
Cuts at: 114 167 535 1918 2140 2604 114
Size: 53 368 1383 222 464 3179
Fragments arranged by size:
3179 1383 464 368 222 53
PstI C_TGCA'G
Cuts at: 713 1244 2069 2716 3401 713
Size: 531 825 647 685 2981
Fragments arranged by size:
2981 825 685 647 531
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5669
PvuII CAG'CTG
Cuts at: 687 1098 1294 3094 3454 687
Size: 411 196 1800 360 2902
Fragments arranged by size:
2902 1800 411 360 196
RsrII CG'GwC_CG
Cuts at: 3864 3864
Size: 5669
SacI G_AGCT'C
Cuts at: 554 2683 554
Size: 2129 3540
SacII CC_GC'GG
Cuts at: 701 800 701
Size: 99 5570
SalI G'TCGA_C
Cuts at: 2706 2706
Size: 5669
SapI GCTCTTCn'nnn_
Cuts at: 3692 3902 3692
Size: 210 5459
SbfI CC_TGCA'GG
Cuts at: 2069 2716 2069
Size: 647 5022
ScaI AGT'ACT
Cuts at: 5186 5186
Size: 5669
SexAI A'CCwGG_T
Cuts at: 295 890 1028 2197 2204 295
Size: 595 138 1169 7 3760
Fragments arranged by size:
3760 1169 595 138 7
SfcI C'TryA_G
Cuts at: 33 709 963 1240 2065 2712 3397 4390 4581
Size: 676 254 277 825 647 685 993 191
Cuts at: 4581 33
Size: 1121
Fragments arranged by size:
1121 993 825 685 676 647 277 254
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 770 1009 770
Size: 239 5430
SfoI GGC'GCC
Cuts at: 2496 3101 3349 2496
Size: 605 248 4816
Fragments arranged by size:
4816 605 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5669
SmaI CCC'GGG
Cuts at: 381 637 2691 381
Size: 256 2054 3359
Fragments arranged by size:
3359 2054 256
SmlI C'TyrA_G
Cuts at: 2824 3739 4231 4493 2824
Size: 915 492 262 4000
Fragments arranged by size:
4000 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5669
SphI G_CATG'C
Cuts at: 2722 3222 2722
Size: 500 5169
SrfI GCCC'GGGC
Cuts at: 381 381
Size: 5669
StuI AGG'CCT
Cuts at: 1012 1909 1012
Size: 897 4772
StyI C'CwwG_G
Cuts at: 461 557 989 2619 2803 3157 461
Size: 96 432 1630 184 354 2973
Fragments arranged by size:
2973 1630 432 354 184 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 720 987 1473 3252 3385 3844 4053 720
Size: 267 486 1779 133 459 209 2336
Fragments arranged by size:
2336 1779 486 459 267 209 133
TatI w'GTAC_w
Cuts at: 1397 5184 1397
Size: 3787 1882
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1754 1786 5186 5218 1754
Size: 32 3400 32 2205
Fragments arranged by size:
3400 2205 32 32
Tth111I GACn'n_nGTC
Cuts at: 1030 3466 1030
Size: 2436 3233
XbaI T'CTAG_A
Cuts at: 2700 2700
Size: 5669
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 996 2259 2322 2463 3164 996
Size: 1263 63 141 701 3501
Fragments arranged by size:
3501 1263 701 141 63
XmaI C'CCGG_G
Cuts at: 379 635 2689 379
Size: 256 2054 3359
Fragments arranged by size:
3359 2054 256
ZraI GAC'GTC
Cuts at: 882 882
Size: 5669
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AloI AscI AvrII BaeI BfrBI
BsiWI BsmI BsmBI BssHII BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PshAI PsiI PspXI PsrI SanDI SgrAI
SnaBI SspI SwaI XhoI XmnI