Restriction map for
pF1KE0077
(Circular) (Six-base) MAPSORT of: pF1KE0077.seq Check: 832 from: 1 to: 6722
pF1KE0077 6722 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 3760 3760
Size: 6722
Acc65I G'GTAC_C
Cuts at: 3738 3738
Size: 6722
AclI AA'CG_TT
Cuts at: 6562 6562
Size: 6722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 1301 1998 2379 4547 4979 5726 5994 217
Size: 1084 697 381 2168 432 747 268 945
Fragments arranged by size:
2168 1084 945 747 697 432 381 268
AfeI AGC'GCT
Cuts at: 2392 2392
Size: 6722
AflIII A'CryG_T
Cuts at: 4074 5178 6155 4074
Size: 1104 977 4641
Fragments arranged by size:
4641 1104 977
AgeI A'CCGG_T
Cuts at: 5110 5110
Size: 6722
AleI CACnn'nnGTG
Cuts at: 2224 2224
Size: 6722
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1084 1250 2104 2467 3299 1084
Size: 166 854 363 832 4507
Fragments arranged by size:
4507 854 832 363 166
AlwNI CAG_nnn'CTG
Cuts at: 234 396 450 771 1482 1820 1845 1857 2979
Size: 162 54 321 711 338 25 12 1122
Cuts at: 2979 3640 3712 4027 5594 234
Size: 661 72 315 1567 1362
Fragments arranged by size:
1567 1362 1122 711 661 338 321 315
162 72 54 25 12
ApaI G_GGCC'C
Cuts at: 312 2562 2898 3454 312
Size: 2250 336 556 3580
Fragments arranged by size:
3580 2250 556 336
ApaLI G'TGCA_C
Cuts at: 2932 3027 5492 2932
Size: 95 2465 4162
Fragments arranged by size:
4162 2465 95
ApoI r'AATT_y
Cuts at: 973 3726 6075 6331 973
Size: 2753 2349 256 1364
Fragments arranged by size:
2753 2349 1364 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6722
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6722
AvaI C'yCGr_G
Cuts at: 141 2261 2665 2897 3742 141
Size: 2120 404 232 845 3121
Fragments arranged by size:
3121 2120 845 404 232
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3460 3493 3460
Size: 33 6689
BamHI G'GATC_C
Cuts at: 257 756 3747 257
Size: 499 2991 3232
Fragments arranged by size:
3232 2991 499
BanI G'GyrC_C
Cuts at: 1558 1603 1775 1977 2256 2266 2436 3619 3738
Size: 45 172 202 279 10 170 1183 119
Cuts at: 3738 4152 4400 4435 1558
Size: 414 248 35 3845
Fragments arranged by size:
3845 1183 414 279 248 202 172 170
119 45 35 10
BanII G_rGCy'C
Cuts at: 312 712 1436 2005 2012 2562 2672 2898 3454
Size: 400 724 569 7 550 110 226 556
Cuts at: 3454 3650 3736 4766 312
Size: 196 86 1030 2268
Fragments arranged by size:
2268 1030 724 569 556 550 400 226
196 110 86 7
BbeI G_GCGC'C
Cuts at: 1779 4156 4404 1779
Size: 2377 248 4097
Fragments arranged by size:
4097 2377 248
BbsI GAAGACnn'nnnn_
Cuts at: 1734 2869 1734
Size: 1135 5587
BbvCI CC'TCA_GC
Cuts at: 1039 1756 1039
Size: 717 6005
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1593 1627 3018 3052 1593
Size: 34 1391 34 5263
Fragments arranged by size:
5263 1391 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4620 5381 6722 4620
Size: 761 1341 4620
Fragments arranged by size:
4620 1341 761
BclI T'GATC_A
Cuts at: 4242 4242
Size: 6722
BglI GCCn_nnn'nGGC
Cuts at: 307 1201 2434 2731 2923 307
Size: 894 1233 297 192 4106
Fragments arranged by size:
4106 1233 894 297 192
BglII A'GATC_T
Cuts at: 4237 6274 4237
Size: 2037 4685
BlpI GC'TnA_GC
Cuts at: 2813 3834 2813
Size: 1021 5701
Bme1580I G_kGCm'C
Cuts at: 312 900 1443 1606 1982 2100 2269 2562 2898
Size: 588 543 163 376 118 169 293 336
Cuts at: 2898 2936 3031 3454 4347 4440 5496 312
Size: 38 95 423 893 93 1056 1538
Fragments arranged by size:
1538 1056 893 588 543 423 376 336
293 169 163 118 95 93 38
BmrI ACTGGGnnnn_n'
Cuts at: 1359 2008 2514 2826 3722 3934 4015 4350 6494
Size: 649 506 312 896 212 81 335 2144
Cuts at: 6494 1359
Size: 1587
Fragments arranged by size:
2144 1587 896 649 506 335 312 212
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 715 5089 715
Size: 4374 2348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 793 1155 1552 1797 2042 2134 2609 2842
Size: 294 362 397 245 245 92 475 233
Cuts at: 2842 499
Size: 4379
Fragments arranged by size:
4379 475 397 362 294 245 245 233
92
Bpu10I CC'TnA_GC
Cuts at: 1039 1756 1829 2162 2203 2287 2299 3529 1039
Size: 717 73 333 41 84 12 1230 4232
Fragments arranged by size:
4232 1230 717 333 84 73 41 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 2389 3292 3898 4777 5269 5567 820
Size: 1569 903 606 879 492 298 1975
Fragments arranged by size:
1975 1569 903 879 606 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 328 1013 6426 328
Size: 685 5413 624
Fragments arranged by size:
5413 685 624
BsaAI yAC'GTr
Cuts at: 849 3213 4075 849
Size: 2364 862 3496
Fragments arranged by size:
3496 2364 862
BsaBI GATnn'nnATC
Cuts at: 6 1840 4236 6273 6
Size: 1834 2396 2037 455
Fragments arranged by size:
2396 2037 1834 455
BsaHI Gr'CG_yC
Cuts at: 1776 2594 4153 4401 5103 6599 1776
Size: 818 1559 248 702 1496 1899
Fragments arranged by size:
1899 1559 1496 818 702 248
BsaWI w'CCGG_w
Cuts at: 3911 4432 5110 5384 5531 5942 3911
Size: 521 678 274 147 411 4691
Fragments arranged by size:
4691 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 491 521 2344 2374 491
Size: 30 1823 30 4839
Fragments arranged by size:
4839 1823 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 439 790 1156 1233 2068 2071 3046 3397 3537
Size: 351 366 77 835 3 975 351 140
Cuts at: 3537 439
Size: 3624
Fragments arranged by size:
3624 975 835 366 351 351 140 77
3
BseYI C'CCAG_C
Cuts at: 156 187 417 780 1002 1223 1746 1857 2294
Size: 31 230 363 222 221 523 111 437
Cuts at: 2294 2926 2944 3318 4147 4905 5482 5978 156
Size: 632 18 374 829 758 577 496 900
Fragments arranged by size:
900 829 758 632 577 523 496 437
374 363 230 222 221 111 31 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 1207 1701 2368 2437 2749 850
Size: 357 494 667 69 312 4823
Fragments arranged by size:
4823 667 494 357 312 69
BsiEI CG_ry'CG
Cuts at: 104 118 729 4264 4310 5518 104
Size: 14 611 3535 46 1208 1308
Fragments arranged by size:
3535 1308 1208 611 46 14
BsiHKAI G_wGCw'C
Cuts at: 284 1063 1802 2012 2183 2193 2672 2936 3031
Size: 779 739 210 171 10 479 264 95
Cuts at: 3031 3048 3278 3392 3736 4514 4704 5496 284
Size: 17 230 114 344 778 190 792 1510
Fragments arranged by size:
1510 792 779 778 739 479 344 264
230 210 190 171 114 95 17 10
BsmBI CGTCTCn'nnnn_
Cuts at: 2585 2585
Size: 6722
Bsp1286I G_dGCh'C
Cuts at: 284 312 712 900 1063 1436 1443 1606 1802
Size: 28 400 188 163 373 7 163 196
Cuts at: 1802 1982 2005 2012 2100 2183 2193 2269 2562
Size: 180 23 7 88 83 10 76 293
Cuts at: 2562 2672 2898 2936 3031 3048 3278 3392 3454
Size: 110 226 38 95 17 230 114 62
Cuts at: 3454 3650 3736 4347 4440 4514 4704 4766 5496
Size: 196 86 611 93 74 190 62 730
Cuts at: 5496 284
Size: 1510
Fragments arranged by size:
1510 730 611 400 373 293 230 226
196 196 190 188 180 163 163 114
110 95 93 88 86 83 76 74
62 62 38 28 23 17 10 7
7
BspEI T'CCGG_A
Cuts at: 5942 5942
Size: 6722
BspHI T'CATG_A
Cuts at: 5898 5898
Size: 6722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 728 3260 3772 3986 4288 4669 728
Size: 2532 512 214 302 381 2781
Fragments arranged by size:
2781 2532 512 381 302 214
BsrBI CCG'CTC
Cuts at: 50 630 5014 5068 6552 6717 50
Size: 580 4384 54 1484 165 55
Fragments arranged by size:
4384 1484 580 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1883 2547 3313 4634 1883
Size: 664 766 1321 3971
Fragments arranged by size:
3971 1321 766 664
BsrFI r'CCGG_y
Cuts at: 2424 2917 4720 4901 5110 2424
Size: 493 1803 181 209 4036
Fragments arranged by size:
4036 1803 493 209 181
BsrGI T'GTAC_A
Cuts at: 3476 3476
Size: 6722
BssSI C'ACGA_G
Cuts at: 937 3418 4993 5351 937
Size: 2481 1575 358 2308
Fragments arranged by size:
2481 2308 1575 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4099 4099
Size: 6722
BstBI TT'CG_AA
Cuts at: 5083 5083
Size: 6722
BstEII G'GTnAC_C
Cuts at: 1947 2331 1947
Size: 384 6338
BstXI CCAn_nnnn'nTGG
Cuts at: 1254 2175 3162 3318 3591 1254
Size: 921 987 156 273 4385
Fragments arranged by size:
4385 987 921 273 156
BstYI r'GATC_y
Cuts at: 1 179 257 756 1229 1841 3494 3747 4214
Size: 178 78 499 473 612 1653 253 467
Cuts at: 4214 4237 4572 4818 5830 5916 5928 6274 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1653 1012 612 499 473 467 449 346
335 253 246 178 86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 1422 1422
Size: 6722
BtgI C'CryG_G
Cuts at: 957 1379 2783 3161 3411 3590 957
Size: 422 1404 378 250 179 4089
Fragments arranged by size:
4089 1404 422 378 250 179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3112 4672 4853 5016 3112
Size: 1560 181 163 4818
Fragments arranged by size:
4818 1560 181 163
BtsI GCAGTG_nn'
Cuts at: 527 882 1779 1895 2709 3965 527
Size: 355 897 116 814 1256 3284
Fragments arranged by size:
3284 1256 897 814 355 116
DraI TTT'AAA
Cuts at: 3721 3721
Size: 6722
DraIII CAC_nnn'GTG
Cuts at: 895 1977 895
Size: 1082 5640
DrdI GACnn_nn'nnGTC
Cuts at: 4428 5286 4428
Size: 858 5864
EaeI y'GGCC_r
Cuts at: 332 726 1013 1637 1700 2426 2919 3005 3593
Size: 394 287 624 63 726 493 86 588
Cuts at: 3593 4307 4481 4872 4899 6244 332
Size: 714 174 391 27 1345 810
Fragments arranged by size:
1345 810 726 714 624 588 493 394
391 287 174 86 63 27
EagI C'GGCC_G
Cuts at: 726 4307 726
Size: 3581 3141
EarI CTCTTCn'nnn_
Cuts at: 244 2017 2198 2677 2968 4745 4955 244
Size: 1773 181 479 291 1777 210 2011
Fragments arranged by size:
2011 1777 1773 479 291 210 181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 702 4930 5240 5386 6532 702
Size: 4228 310 146 1146 892
Fragments arranged by size:
4228 1146 892 310 146
EcoICRI GAG'CTC
Cuts at: 2010 2670 3734 2010
Size: 660 1064 4998
Fragments arranged by size:
4998 1064 660
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 499 793 1155 1301 1552 1797 1998 2042
Size: 282 294 362 146 251 245 201 44
Cuts at: 2042 2134 2379 2609 2842 4547 4979 5726 5994
Size: 92 245 230 233 1705 432 747 268
Cuts at: 5994 217
Size: 945
Fragments arranged by size:
1705 945 747 432 362 294 282 268
251 245 245 233 230 201 146 92
44
EcoNI CCTnn'n_nnAGG
Cuts at: 1849 2317 1849
Size: 468 6254
EcoO109I rG'GnC_Cy
Cuts at: 231 308 309 784 1050 1203 2037 2558 2559
Size: 77 1 475 266 153 834 521 1
Cuts at: 2559 2894 3861 231
Size: 335 967 3092
Fragments arranged by size:
3092 967 834 521 475 335 266 153
77 1 1
EcoRI G'AATT_C
Cuts at: 3726 3726
Size: 6722
EcoRV GAT'ATC
Cuts at: 738 738
Size: 6722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4741 4741
Size: 6722
FseI GG_CCGG'CC
Cuts at: 2921 2921
Size: 6722
FspI TGC'GCA
Cuts at: 2910 4503 2910
Size: 1593 5129
HaeII r_GCGC'y
Cuts at: 1182 1779 1791 2294 2394 2994 3382 4156 4404
Size: 597 12 503 100 600 388 774 248
Cuts at: 4404 5426 6405 1182
Size: 1022 979 1499
Fragments arranged by size:
1499 1022 979 774 600 597 503 388
248 100 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 242 274 467 499 3486 3518 4564 4594 4596
Size: 32 193 32 2987 32 1046 30 2
Cuts at: 4596 4626 242
Size: 30 2338
Fragments arranged by size:
2987 2338 1046 193 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3761 3761
Size: 6722
KasI G'GCGC_C
Cuts at: 1775 4152 4400 1775
Size: 2377 248 4097
Fragments arranged by size:
4097 2377 248
KpnI G_GTAC'C
Cuts at: 3742 3742
Size: 6722
MluI A'CGCG_T
Cuts at: 6155 6155
Size: 6722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5393 5577 5393
Size: 184 6538
MscI TGG'CCA
Cuts at: 334 1015 3595 4483 334
Size: 681 2580 888 2573
Fragments arranged by size:
2580 2573 888 681
MslI CAynn'nnrTG
Cuts at: 872 2224 3160 3589 4711 4838 872
Size: 1352 936 429 1122 127 2756
Fragments arranged by size:
2756 1352 1122 936 429 127
MspA1I CmG'CkG
Cuts at: 768 1089 1176 1191 2102 2622 2948 3833 4147
Size: 321 87 15 911 520 326 885 314
Cuts at: 4147 4507 5520 5765 768
Size: 360 1013 245 1725
Fragments arranged by size:
1725 1013 911 885 520 360 326 321
314 245 87 15
NaeI GCC'GGC
Cuts at: 2919 4903 2919
Size: 1984 4738
NarI GG'CG_CC
Cuts at: 1776 4153 4401 1776
Size: 2377 248 4097
Fragments arranged by size:
4097 2377 248
NcoI C'CATG_G
Cuts at: 957 3411 957
Size: 2454 4268
NgoMIV G'CCGG_C
Cuts at: 2917 4901 2917
Size: 1984 4738
NspI r_CATG'y
Cuts at: 1273 1537 1945 2161 2281 2909 3775 4275 5182
Size: 264 408 216 120 628 866 500 907
Cuts at: 5182 1273
Size: 2813
Fragments arranged by size:
2813 907 866 628 500 408 264 216
120
PasI CC'CwG_GG
Cuts at: 1371 2337 2712 3237 1371
Size: 966 375 525 4856
Fragments arranged by size:
4856 966 525 375
PciI A'CATG_T
Cuts at: 5178 5178
Size: 6722
PflMI CCAn_nnn'nTGG
Cuts at: 1857 1944 3712 1857
Size: 87 1768 4867
Fragments arranged by size:
4867 1768 87
PfoI T'CCnGG_A
Cuts at: 809 1568 809
Size: 759 5963
PmeI GTTT'AAAC
Cuts at: 3721 3721
Size: 6722
PmlI CAC'GTG
Cuts at: 3213 3213
Size: 6722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5861 5893 5861
Size: 32 6690
PpuMI rG'GwC_Cy
Cuts at: 784 2037 784
Size: 1253 5469
PspOMI G'GGCC_C
Cuts at: 308 2558 2894 3450 308
Size: 2250 336 556 3580
Fragments arranged by size:
3580 2250 556 336
PstI C_TGCA'G
Cuts at: 1058 1595 2871 3769 4454 1058
Size: 537 1276 898 685 3326
Fragments arranged by size:
3326 1276 898 685 537
PvuI CG_AT'CG
Cuts at: 104 118 104
Size: 14 6708
PvuII CAG'CTG
Cuts at: 768 1089 1191 2622 4147 4507 768
Size: 321 102 1431 1525 360 2983
Fragments arranged by size:
2983 1525 1431 360 321 102
RsrII CG'GwC_CG
Cuts at: 4917 4917
Size: 6722
SacI G_AGCT'C
Cuts at: 2012 2672 3736 2012
Size: 660 1064 4998
Fragments arranged by size:
4998 1064 660
SalI G'TCGA_C
Cuts at: 3759 3759
Size: 6722
SanDI GG'GwC_CC
Cuts at: 784 784
Size: 6722
SapI GCTCTTCn'nnn_
Cuts at: 244 2017 2198 2677 4745 4955 244
Size: 1773 181 479 2068 210 2011
Fragments arranged by size:
2068 2011 1773 479 210 181
SbfI CC_TGCA'GG
Cuts at: 3769 3769
Size: 6722
ScaI AGT'ACT
Cuts at: 6239 6239
Size: 6722
SexAI A'CCwGG_T
Cuts at: 1973 3699 1973
Size: 1726 4996
SfcI C'TryA_G
Cuts at: 33 1054 1591 1693 2473 2867 3182 3765 4450
Size: 1021 537 102 780 394 315 583 685
Cuts at: 4450 5443 5634 33
Size: 993 191 1121
Fragments arranged by size:
1121 1021 993 780 685 583 537 394
315 191 102
SfiI GGCCn_nnn'nGGCC
Cuts at: 307 1201 307
Size: 894 5828
SfoI GGC'GCC
Cuts at: 1777 4154 4402 1777
Size: 2377 248 4097
Fragments arranged by size:
4097 2377 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6722
SmaI CCC'GGG
Cuts at: 2899 3744 2899
Size: 845 5877
SmlI C'TyrA_G
Cuts at: 799 2404 3271 3877 4792 5284 5546 799
Size: 1605 867 606 915 492 262 1975
Fragments arranged by size:
1975 1605 915 867 606 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6722
SphI G_CATG'C
Cuts at: 1273 1537 2281 2909 3775 4275 1273
Size: 264 744 628 866 500 3720
Fragments arranged by size:
3720 866 744 628 500 264
SrfI GCCC'GGGC
Cuts at: 2899 2899
Size: 6722
StuI AGG'CCT
Cuts at: 844 1427 844
Size: 583 6139
StyI C'CwwG_G
Cuts at: 407 702 957 1026 1428 2222 2270 2819 3411
Size: 295 255 69 402 794 48 549 592
Cuts at: 3411 3652 3856 4210 407
Size: 241 204 354 2919
Fragments arranged by size:
2919 794 592 549 402 354 295 255
241 204 69 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2446 2685 3250 3310 3617 4305 4438 4897 5106
Size: 239 565 60 307 688 133 459 209
Cuts at: 5106 2446
Size: 4062
Fragments arranged by size:
4062 688 565 459 307 239 209 133
60
TatI w'GTAC_w
Cuts at: 3476 6237 3476
Size: 2761 3961
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 164 196 491 523 727 759 1493 1525 6239
Size: 32 295 32 204 32 734 32 4714
Cuts at: 6239 6271 164
Size: 32 615
Fragments arranged by size:
4714 734 615 295 204 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3701 4519 3701
Size: 818 5904
XbaI T'CTAG_A
Cuts at: 3753 3753
Size: 6722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 184 268 414 1632 1758 2634 2756 4217 184
Size: 84 146 1218 126 876 122 1461 2689
Fragments arranged by size:
2689 1461 1218 876 146 126 122 84
XmaI C'CCGG_G
Cuts at: 2897 3742 2897
Size: 845 5877
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI AvrII BfrBI
BmgBI BmtI BsiWI BsmI BssHII BstZ17I ClaI CspCI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PspXI PsrI SacII SgrAI
SnaBI SspI SwaI XhoI XmnI ZraI