Restriction map for
pF1KE0072
(Circular) (Six-base) MAPSORT of: pF1KE0072.seq Check: 6267 from: 1 to: 7133
pF1KE0072 7133 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3269 3269
Size: 7133
AccI GT'mk_AC
Cuts at: 975 4171 975
Size: 3196 3937
Acc65I G'GTAC_C
Cuts at: 1842 3210 4149 1842
Size: 1368 939 4826
Fragments arranged by size:
4826 1368 939
AclI AA'CG_TT
Cuts at: 6973 6973
Size: 7133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 986 2475 3425 4958 5390 6137 6405 986
Size: 1489 950 1533 432 747 268 1714
Fragments arranged by size:
1714 1533 1489 950 747 432 268
AflII C'TTAA_G
Cuts at: 2728 2728
Size: 7133
AflIII A'CryG_T
Cuts at: 4485 5589 6566 4485
Size: 1104 977 5052
Fragments arranged by size:
5052 1104 977
AgeI A'CCGG_T
Cuts at: 5521 5521
Size: 7133
AhdI GACnn_n'nnGTC
Cuts at: 1482 1482
Size: 7133
AleI CACnn'nnGTG
Cuts at: 1003 3118 1003
Size: 2115 5018
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3788 3998 3788
Size: 210 6923
AlwNI CAG_nnn'CTG
Cuts at: 691 1030 1525 4438 6005 691
Size: 339 495 2913 1567 1819
Fragments arranged by size:
2913 1819 1567 495 339
ApaLI G'TGCA_C
Cuts at: 5903 5903
Size: 7133
ApoI r'AATT_y
Cuts at: 302 1412 1738 1930 2754 3050 3072 4137 6486
Size: 1110 326 192 824 296 22 1065 2349
Cuts at: 6486 6742 302
Size: 256 693
Fragments arranged by size:
2349 1110 1065 824 693 326 296 256
192 22
AseI AT'TA_AT
Cuts at: 20 2549 20
Size: 2529 4604
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7133
AvaI C'yCGr_G
Cuts at: 4153 4153
Size: 7133
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 833 866 3202 3235 3718 3751 833
Size: 33 2336 33 483 33 4215
Fragments arranged by size:
4215 2336 483 33 33 33
BamHI G'GATC_C
Cuts at: 4158 4158
Size: 7133
BanI G'GyrC_C
Cuts at: 268 1842 2662 2718 3210 4149 4563 4811 4846
Size: 1574 820 56 492 939 414 248 35
Cuts at: 4846 268
Size: 2555
Fragments arranged by size:
2555 1574 939 820 492 414 248 56
35
BanII G_rGCy'C
Cuts at: 4147 5177 4147
Size: 1030 6103
BbeI G_GCGC'C
Cuts at: 272 4567 4815 272
Size: 4295 248 2590
Fragments arranged by size:
4295 2590 248
BbvCI CC'TCA_GC
Cuts at: 2138 3807 2138
Size: 1669 5464
BciVI GTATCCnnnnn_n'
Cuts at: 435 511 834 2622 5031 5792 7133 435
Size: 76 323 1788 2409 761 1341 435
Fragments arranged by size:
2409 1788 1341 761 435 323 76
BclI T'GATC_A
Cuts at: 1887 3963 4653 1887
Size: 2076 690 4367
Fragments arranged by size:
4367 2076 690
BglII A'GATC_T
Cuts at: 452 2367 4648 6685 452
Size: 1915 2281 2037 900
Fragments arranged by size:
2281 2037 1915 900
BlpI GC'TnA_GC
Cuts at: 4245 4245
Size: 7133
Bme1580I G_kGCm'C
Cuts at: 4758 4851 5907 4758
Size: 93 1056 5984
Fragments arranged by size:
5984 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 937 1599 1900 3310 4019 4345 4426 4761 6905
Size: 662 301 1410 709 326 81 335 2144
Cuts at: 6905 937
Size: 1165
Fragments arranged by size:
2144 1410 1165 709 662 335 326 301
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1656 5500 1656
Size: 3844 3289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1477 1577 3668 1477
Size: 100 2091 4942
Fragments arranged by size:
4942 2091 100
Bpu10I CC'TnA_GC
Cuts at: 2138 3807 2138
Size: 1669 5464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3135 3491 4309 5188 5680 5978 3135
Size: 356 818 879 492 298 4290
Fragments arranged by size:
4290 879 818 492 356 298
BsaI GGTCTCn'nnnn_
Cuts at: 3299 6837 3299
Size: 3538 3595
BsaAI yAC'GTr
Cuts at: 4486 4486
Size: 7133
BsaBI GATnn'nnATC
Cuts at: 6 4647 6684 6
Size: 4641 2037 455
Fragments arranged by size:
4641 2037 455
BsaHI Gr'CG_yC
Cuts at: 269 4564 4812 5514 7010 269
Size: 4295 248 702 1496 392
Fragments arranged by size:
4295 1496 702 392 248
BsaWI w'CCGG_w
Cuts at: 409 4322 4843 5521 5795 5942 6353 409
Size: 3913 521 678 274 147 411 1189
Fragments arranged by size:
3913 1189 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3121 3151 4050 4080 3121
Size: 30 899 30 6174
Fragments arranged by size:
6174 899 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 146 3382 3746 146
Size: 3236 364 3533
Fragments arranged by size:
3533 3236 364
BseYI C'CCAG_C
Cuts at: 621 684 3239 3391 4558 5316 5893 6389 621
Size: 63 2555 152 1167 758 577 496 1365
Fragments arranged by size:
2555 1365 1167 758 577 496 152 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3486 3758 3784 3486
Size: 272 26 6835
Fragments arranged by size:
6835 272 26
BsiEI CG_ry'CG
Cuts at: 104 4675 4721 5929 104
Size: 4571 46 1208 1308
Fragments arranged by size:
4571 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 4147 4925 5115 5907 4147
Size: 778 190 792 5373
Fragments arranged by size:
5373 792 778 190
BsmI GAATG_Cn'
Cuts at: 1467 2331 2829 1467
Size: 864 498 5771
Fragments arranged by size:
5771 864 498
Bsp1286I G_dGCh'C
Cuts at: 4147 4758 4851 4925 5115 5177 5907 4147
Size: 611 93 74 190 62 730 5373
Fragments arranged by size:
5373 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 6353 6353
Size: 7133
BspHI T'CATG_A
Cuts at: 1407 6309 1407
Size: 4902 2231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1721 2852 3269 4183 4397 4699 5080 1721
Size: 1131 417 914 214 302 381 3774
Fragments arranged by size:
3774 1131 914 417 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5425 5479 6963 7128 50
Size: 5375 54 1484 165 55
Fragments arranged by size:
5375 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 516 1085 1226 1559 1866 5045 516
Size: 569 141 333 307 3179 2604
Fragments arranged by size:
3179 2604 569 333 307 141
BsrFI r'CCGG_y
Cuts at: 463 3549 5131 5312 5521 463
Size: 3086 1582 181 209 2075
Fragments arranged by size:
3086 2075 1582 209 181
BsrGI T'GTAC_A
Cuts at: 401 401
Size: 7133
BssSI C'ACGA_G
Cuts at: 5404 5762 5404
Size: 358 6775
BstAPI GCAn_nnn'nTGC
Cuts at: 1678 2656 3841 4510 1678
Size: 978 1185 669 4301
Fragments arranged by size:
4301 1185 978 669
BstBI TT'CG_AA
Cuts at: 429 5494 429
Size: 5065 2068
BstXI CCAn_nnnn'nTGG
Cuts at: 2190 2190
Size: 7133
BstYI r'GATC_y
Cuts at: 1 452 889 1052 2367 2639 3162 4158 4625
Size: 451 437 163 1315 272 523 996 467
Cuts at: 4625 4648 4983 5229 6241 6327 6339 6685 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1315 1012 996 523 467 451 449 437
346 335 272 246 163 86 23 12
BstZ17I GTA'TAC
Cuts at: 976 976
Size: 7133
Bsu36I CC'TnA_GG
Cuts at: 3362 3362
Size: 7133
BtgI C'CryG_G
Cuts at: 3339 3339
Size: 7133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3684 5083 5264 5427 3684
Size: 1399 181 163 5390
Fragments arranged by size:
5390 1399 181 163
BtsI GCAGTG_nn'
Cuts at: 2693 3294 4376 2693
Size: 601 1082 5450
Fragments arranged by size:
5450 1082 601
DraI TTT'AAA
Cuts at: 4132 4132
Size: 7133
DraIII CAC_nnn'GTG
Cuts at: 2448 2448
Size: 7133
DrdI GACnn_nn'nnGTC
Cuts at: 4839 5697 4839
Size: 858 6275
EaeI y'GGCC_r
Cuts at: 3525 4718 4892 5283 5310 6655 3525
Size: 1193 174 391 27 1345 4003
Fragments arranged by size:
4003 1345 1193 391 174 27
EagI C'GGCC_G
Cuts at: 4718 4718
Size: 7133
EarI CTCTTCn'nnn_
Cuts at: 150 164 431 827 1564 2494 2933 3479 3579
Size: 14 267 396 737 930 439 546 100
Cuts at: 3579 3867 5156 5366 150
Size: 288 1289 210 1917
Fragments arranged by size:
1917 1289 930 737 546 439 396 288
267 210 100 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5341 5651 5797 6943 5341
Size: 310 146 1146 5531
Fragments arranged by size:
5531 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4145 4145
Size: 7133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 986 1477 1577 2475 3425 3668 4958 5390 6137
Size: 491 100 898 950 243 1290 432 747
Cuts at: 6137 6405 986
Size: 268 1714
Fragments arranged by size:
1714 1290 950 898 747 491 432 268
243 100
EcoNI CCTnn'n_nnAGG
Cuts at: 704 704
Size: 7133
EcoO109I rG'GnC_Cy
Cuts at: 1720 4272 1720
Size: 2552 4581
EcoRI G'AATT_C
Cuts at: 1738 2754 4137 1738
Size: 1016 1383 4734
Fragments arranged by size:
4734 1383 1016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1074 5152 1074
Size: 4078 3055
FspI TGC'GCA
Cuts at: 2563 4914 2563
Size: 2351 4782
HaeII r_GCGC'y
Cuts at: 272 470 4567 4815 5837 6816 272
Size: 198 4097 248 1022 979 589
Fragments arranged by size:
4097 1022 979 589 248 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1268 1300 1468 1500 2438 2470 2599 2631 3121
Size: 32 168 32 938 32 129 32 490
Cuts at: 3121 3153 3403 3415 3435 3447 4975 5005 5007
Size: 32 250 12 20 12 1528 30 2
Cuts at: 5007 5037 1268
Size: 30 3364
Fragments arranged by size:
3364 1528 938 490 250 168 129 32
32 32 32 32 30 30 20 12
12 2
HincII GTy'rAC
Cuts at: 2307 2892 4172 2307
Size: 585 1280 5268
Fragments arranged by size:
5268 1280 585
KasI G'GCGC_C
Cuts at: 268 4563 4811 268
Size: 4295 248 2590
Fragments arranged by size:
4295 2590 248
KpnI G_GTAC'C
Cuts at: 1846 3214 4153 1846
Size: 1368 939 4826
Fragments arranged by size:
4826 1368 939
MfeI C'AATT_G
Cuts at: 2961 3141 2961
Size: 180 6953
MluI A'CGCG_T
Cuts at: 6566 6566
Size: 7133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1106 2103 5804 5988 1106
Size: 997 3701 184 2251
Fragments arranged by size:
3701 2251 997 184
MscI TGG'CCA
Cuts at: 3527 4894 3527
Size: 1367 5766
MslI CAynn'nnrTG
Cuts at: 1003 1109 1354 1567 2188 2320 3118 3533 4025
Size: 106 245 213 621 132 798 415 492
Cuts at: 4025 5122 5249 1003
Size: 1097 127 2887
Fragments arranged by size:
2887 1097 798 621 492 415 245 213
132 127 106
MspA1I CmG'CkG
Cuts at: 688 2653 3243 4244 4558 4918 5931 6176 688
Size: 1965 590 1001 314 360 1013 245 1645
Fragments arranged by size:
1965 1645 1013 1001 590 360 314 245
NaeI GCC'GGC
Cuts at: 3551 5314 3551
Size: 1763 5370
NarI GG'CG_CC
Cuts at: 269 4564 4812 269
Size: 4295 248 2590
Fragments arranged by size:
4295 2590 248
NcoI C'CATG_G
Cuts at: 3339 3339
Size: 7133
NgoMIV G'CCGG_C
Cuts at: 3549 5312 3549
Size: 1763 5370
NspI r_CATG'y
Cuts at: 1397 1767 2751 4186 4686 5593 1397
Size: 370 984 1435 500 907 2937
Fragments arranged by size:
2937 1435 984 907 500 370
PciI A'CATG_T
Cuts at: 5589 5589
Size: 7133
PflMI CCAn_nnn'nTGG
Cuts at: 1960 1960
Size: 7133
PfoI T'CCnGG_A
Cuts at: 217 1454 217
Size: 1237 5896
PmeI GTTT'AAAC
Cuts at: 4132 4132
Size: 7133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3089 3121 6272 6304 3089
Size: 32 3151 32 3918
Fragments arranged by size:
3918 3151 32 32
PstI C_TGCA'G
Cuts at: 1521 2016 4180 4865 1521
Size: 495 2164 685 3789
Fragments arranged by size:
3789 2164 685 495
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7133
PvuII CAG'CTG
Cuts at: 2653 4558 4918 2653
Size: 1905 360 4868
Fragments arranged by size:
4868 1905 360
RsrII CG'GwC_CG
Cuts at: 5328 5328
Size: 7133
SacI G_AGCT'C
Cuts at: 4147 4147
Size: 7133
SalI G'TCGA_C
Cuts at: 4170 4170
Size: 7133
SapI GCTCTTCn'nnn_
Cuts at: 1564 2933 3579 3867 5156 5366 1564
Size: 1369 646 288 1289 210 3331
Fragments arranged by size:
3331 1369 1289 646 288 210
SbfI CC_TGCA'GG
Cuts at: 4180 4180
Size: 7133
ScaI AGT'ACT
Cuts at: 897 3373 6650 897
Size: 2476 3277 1380
Fragments arranged by size:
3277 2476 1380
SfcI C'TryA_G
Cuts at: 33 992 1517 1772 2012 2699 3168 3819 4176
Size: 959 525 255 240 687 469 651 357
Cuts at: 4176 4861 5854 6045 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 959 687 685 651 525 469
357 255 240 191
SfoI GGC'GCC
Cuts at: 270 4565 4813 270
Size: 4295 248 2590
Fragments arranged by size:
4295 2590 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7133
SmaI CCC'GGG
Cuts at: 4155 4155
Size: 7133
SmlI C'TyrA_G
Cuts at: 2728 3150 3506 4288 5203 5695 5957 2728
Size: 422 356 782 915 492 262 3904
Fragments arranged by size:
3904 915 782 492 422 356 262
SpeI A'CTAG_T
Cuts at: 67 2623 67
Size: 2556 4577
SphI G_CATG'C
Cuts at: 1767 4186 4686 1767
Size: 2419 500 4214
Fragments arranged by size:
4214 2419 500
SspI AAT'ATT
Cuts at: 560 1323 1810 2088 560
Size: 763 487 278 5605
Fragments arranged by size:
5605 763 487 278
StuI AGG'CCT
Cuts at: 3490 3589 3625 3805 3490
Size: 99 36 180 6818
Fragments arranged by size:
6818 180 99 36
StyI C'CwwG_G
Cuts at: 505 1954 2433 3339 3351 4124 4267 4621 505
Size: 1449 479 906 12 773 143 354 3017
Fragments arranged by size:
3017 1449 906 773 479 354 143 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 956 1618 3405 3921 4716 4849 5308 5517 956
Size: 662 1787 516 795 133 459 209 2572
Fragments arranged by size:
2572 1787 795 662 516 459 209 133
TatI w'GTAC_w
Cuts at: 401 895 2957 3371 6648 401
Size: 494 2062 414 3277 886
Fragments arranged by size:
3277 2062 886 494 414
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 602 634 6650 6682 602
Size: 32 6016 32 1053
Fragments arranged by size:
6016 1053 32 32
Tth111I GACn'n_nGTC
Cuts at: 2632 4930 2632
Size: 2298 4835
XbaI T'CTAG_A
Cuts at: 2934 4164 2934
Size: 1230 5903
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1509 4628 1509
Size: 3119 4014
XmaI C'CCGG_G
Cuts at: 4153 4153
Size: 7133
XmnI GAAnn'nnTTC
Cuts at: 302 1322 1949 2255 4016 302
Size: 1020 627 306 1761 3419
Fragments arranged by size:
3419 1761 1020 627 306
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbvCI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AloI ApaI AscI AvrII BbsI BcgI
BfrBI BglI BmgBI BmtI BsiWI BsmBI BssHII BstEII
ClaI CspCI EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI