Restriction map for
pF1KE0071
(Circular) (Six-base) MAPSORT of: pF1KE0071.seq Check: 8029 from: 1 to: 5282
pF1KE0071 5282 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 916 1024 1087 1486 1519 1547 1600 916
Size: 108 63 399 33 28 53 4598
Fragments arranged by size:
4598 399 108 63 53 33 28
AccI GT'mk_AC
Cuts at: 374 2320 374
Size: 1946 3336
Acc65I G'GTAC_C
Cuts at: 2298 2298
Size: 5282
AclI AA'CG_TT
Cuts at: 5122 5122
Size: 5282
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 366 3107 3539 4286 4554 208
Size: 158 2741 432 747 268 936
Fragments arranged by size:
2741 936 747 432 268 158
AflIII A'CryG_T
Cuts at: 2634 3738 4715 2634
Size: 1104 977 3201
Fragments arranged by size:
3201 1104 977
AgeI A'CCGG_T
Cuts at: 3670 3670
Size: 5282
AleI CACnn'nnGTG
Cuts at: 264 361 264
Size: 97 5185
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1141 1173 1486 1518 1141
Size: 32 313 32 4905
Fragments arranged by size:
4905 313 32 32
AlwNI CAG_nnn'CTG
Cuts at: 594 972 1538 1662 2587 4154 594
Size: 378 566 124 925 1567 1722
Fragments arranged by size:
1722 1567 925 566 378 124
ApaI G_GGCC'C
Cuts at: 764 1687 764
Size: 923 4359
ApaLI G'TGCA_C
Cuts at: 4052 4052
Size: 5282
ApoI r'AATT_y
Cuts at: 2286 4635 4891 2286
Size: 2349 256 2677
Fragments arranged by size:
2677 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5282
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5282
AvaI C'yCGr_G
Cuts at: 325 689 1144 1413 1631 1669 1680 2187 2302
Size: 364 455 269 218 38 11 507 115
Cuts at: 2302 325
Size: 3305
Fragments arranged by size:
3305 507 455 364 269 218 115 38
11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 861 894 1056 1089 1146 1179 861
Size: 33 162 33 57 33 4964
Fragments arranged by size:
4964 162 57 33 33 33
BamHI G'GATC_C
Cuts at: 2307 2307
Size: 5282
BanI G'GyrC_C
Cuts at: 403 460 560 904 1072 1357 1474 2298 2712
Size: 57 100 344 168 285 117 824 414
Cuts at: 2712 2960 2995 403
Size: 248 35 2690
Fragments arranged by size:
2690 824 414 344 285 248 168 117
100 57 35
BanII G_rGCy'C
Cuts at: 172 764 1687 2116 2296 3326 172
Size: 592 923 429 180 1030 2128
Fragments arranged by size:
2128 1030 923 592 429 180
BbeI G_GCGC'C
Cuts at: 407 2716 2964 407
Size: 2309 248 2725
Fragments arranged by size:
2725 2309 248
BbvCI CC'TCA_GC
Cuts at: 1819 1819
Size: 5282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 768 802 1125 1159 768
Size: 34 323 34 4891
Fragments arranged by size:
4891 323 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 889 3180 3941 5282 889
Size: 2291 761 1341 889
Fragments arranged by size:
2291 1341 889 761
BclI T'GATC_A
Cuts at: 2802 2802
Size: 5282
BglI GCCn_nnn'nGGC
Cuts at: 248 941 1175 1292 1406 1487 1520 248
Size: 693 234 117 114 81 33 4010
Fragments arranged by size:
4010 693 234 117 114 81 33
BglII A'GATC_T
Cuts at: 2797 4834 2797
Size: 2037 3245
BlpI GC'TnA_GC
Cuts at: 2394 2394
Size: 5282
Bme1580I G_kGCm'C
Cuts at: 463 764 1477 1548 1632 1687 2907 3000 4056
Size: 301 713 71 84 55 1220 93 1056
Cuts at: 4056 463
Size: 1689
Fragments arranged by size:
1689 1220 1056 713 301 93 84 71
55
BmrI ACTGGGnnnn_n'
Cuts at: 2494 2575 2910 5054 2494
Size: 81 335 2144 2722
Fragments arranged by size:
2722 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 687 3649 687
Size: 2962 2320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 186
Size: 5282
Bpu10I CC'TnA_GC
Cuts at: 1819 1819
Size: 5282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 604 2032 2458 3337 3829 4127 283
Size: 321 1428 426 879 492 298 1438
Fragments arranged by size:
1438 1428 879 492 426 321 298
BsaI GGTCTCn'nnnn_
Cuts at: 1864 4986 1864
Size: 3122 2160
BsaAI yAC'GTr
Cuts at: 2635 2635
Size: 5282
BsaBI GATnn'nnATC
Cuts at: 6 2796 4833 6
Size: 2790 2037 455
Fragments arranged by size:
2790 2037 455
BsaHI Gr'CG_yC
Cuts at: 371 404 2713 2961 3663 5159 371
Size: 33 2309 248 702 1496 494
Fragments arranged by size:
2309 1496 702 494 248 33
BsaWI w'CCGG_w
Cuts at: 2471 2992 3670 3944 4091 4502 2471
Size: 521 678 274 147 411 3251
Fragments arranged by size:
3251 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 483 513 724 754 1141 1171 1410 1435 1440
Size: 30 211 30 387 30 239 25 5
Cuts at: 1440 1465 1486 1516 483
Size: 25 21 30 4249
Fragments arranged by size:
4249 387 239 211 30 30 30 30
25 25 21 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 592 1443 2106 2140 2257 592
Size: 851 663 34 117 3617
Fragments arranged by size:
3617 851 663 117 34
BseYI C'CCAG_C
Cuts at: 222 366 944 1086 1108 1686 1785 1842 2016
Size: 144 578 142 22 578 99 57 174
Cuts at: 2016 2707 3465 4042 4538 222
Size: 691 758 577 496 966
Fragments arranged by size:
966 758 691 578 578 577 496 174
144 142 99 57 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 580 753 861 966 1002 1119 1491 2059
Size: 259 173 108 105 36 117 372 568
Cuts at: 2059 321
Size: 3544
Fragments arranged by size:
3544 568 372 259 173 117 108 105
36
BsiEI CG_ry'CG
Cuts at: 104 609 2824 2870 4078 104
Size: 505 2215 46 1208 1308
Fragments arranged by size:
2215 1308 1208 505 46
BsiHKAI G_wGCw'C
Cuts at: 143 172 1252 1568 2296 3074 3264 4056 143
Size: 29 1080 316 728 778 190 792 1369
Fragments arranged by size:
1369 1080 792 778 728 316 190 29
BsmI GAATG_Cn'
Cuts at: 838 838
Size: 5282
BsmBI CGTCTCn'nnnn_
Cuts at: 1858 1858
Size: 5282
Bsp1286I G_dGCh'C
Cuts at: 143 172 463 764 1252 1477 1548 1568 1632
Size: 29 291 301 488 225 71 20 64
Cuts at: 1632 1687 2116 2296 2907 3000 3074 3264 3326
Size: 55 429 180 611 93 74 190 62
Cuts at: 3326 4056 143
Size: 730 1369
Fragments arranged by size:
1369 730 611 488 429 301 291 225
190 180 93 74 71 64 62 55
29 20
BspEI T'CCGG_A
Cuts at: 4502 4502
Size: 5282
BspHI T'CATG_A
Cuts at: 4458 4458
Size: 5282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 602 916 1024 1087 1174 1486 1519 1547 1600
Size: 314 108 63 87 312 33 28 53
Cuts at: 1600 2332 2546 2848 3229 602
Size: 732 214 302 381 2655
Fragments arranged by size:
2655 732 381 314 312 302 214 108
87 63 53 33 28
BsrBI CCG'CTC
Cuts at: 50 3574 3628 5112 5277 50
Size: 3524 54 1484 165 55
Fragments arranged by size:
3524 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3194 3194
Size: 5282
BsrFI r'CCGG_y
Cuts at: 860 1055 1405 1835 3280 3461 3670 860
Size: 195 350 430 1445 181 209 2472
Fragments arranged by size:
2472 1445 430 350 209 195 181
BssHII G'CGCG_C
Cuts at: 122 122
Size: 5282
BssSI C'ACGA_G
Cuts at: 1597 2035 3553 3911 1597
Size: 438 1518 358 2968
Fragments arranged by size:
2968 1518 438 358
BstAPI GCAn_nnn'nTGC
Cuts at: 119 239 1538 2659 119
Size: 120 1299 1121 2742
Fragments arranged by size:
2742 1299 1121 120
BstBI TT'CG_AA
Cuts at: 182 3643 182
Size: 3461 1821
BstXI CCAn_nnnn'nTGG
Cuts at: 1368 1739 1368
Size: 371 4911
BstYI r'GATC_y
Cuts at: 1 1140 2307 2774 2797 3132 3378 4390 4476
Size: 1139 1167 467 23 335 246 1012 86
Cuts at: 4476 4488 4834 1
Size: 12 346 449
Fragments arranged by size:
1167 1139 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2118 2118
Size: 5282
BtgI C'CryG_G
Cuts at: 107 243 900 1289 1971 107
Size: 136 657 389 682 3418
Fragments arranged by size:
3418 682 657 389 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3232 3413 3576 3232
Size: 181 163 4938
Fragments arranged by size:
4938 181 163
BtsI GCAGTG_nn'
Cuts at: 931 1548 2525 931
Size: 617 977 3688
Fragments arranged by size:
3688 977 617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2255 2290 2255
Size: 35 5247
DraI TTT'AAA
Cuts at: 2281 2281
Size: 5282
DraIII CAC_nnn'GTG
Cuts at: 2099 2099
Size: 5282
DrdI GACnn_nn'nnGTC
Cuts at: 1915 2065 2988 3846 1915
Size: 150 923 858 3351
Fragments arranged by size:
3351 923 858 150
EaeI y'GGCC_r
Cuts at: 454 548 606 705 1745 1886 2867 3041 3432
Size: 94 58 99 1040 141 981 174 391
Cuts at: 3432 3459 4804 454
Size: 27 1345 932
Fragments arranged by size:
1345 1040 981 932 391 174 141 99
94 58 27
EagI C'GGCC_G
Cuts at: 606 2867 606
Size: 2261 3021
EarI CTCTTCn'nnn_
Cuts at: 1344 3305 3515 1344
Size: 1961 210 3111
Fragments arranged by size:
3111 1961 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1978 3490 3800 3946 5092 1978
Size: 1512 310 146 1146 2168
Fragments arranged by size:
2168 1512 1146 310 146
EcoICRI GAG'CTC
Cuts at: 170 2294 170
Size: 2124 3158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 208 366 3107 3539 4286 4554 186
Size: 22 158 2741 432 747 268 914
Fragments arranged by size:
2741 914 747 432 268 158 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2038 2038
Size: 5282
EcoO109I rG'GnC_Cy
Cuts at: 591 760 761 951 969 1645 2043 2135 2421
Size: 169 1 190 18 676 398 92 286
Cuts at: 2421 591
Size: 3452
Fragments arranged by size:
3452 676 398 286 190 169 92 18
1
EcoRI G'AATT_C
Cuts at: 2286 2286
Size: 5282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1983 2016 3301 1983
Size: 33 1285 3964
Fragments arranged by size:
3964 1285 33
FspI TGC'GCA
Cuts at: 3063 3063
Size: 5282
HaeII r_GCGC'y
Cuts at: 353 407 1988 2716 2964 3986 4965 353
Size: 54 1581 728 248 1022 979 670
Fragments arranged by size:
1581 1022 979 728 670 248 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 568 600 1435 1467 3124 3154 3156 3186 568
Size: 32 835 32 1657 30 2 30 2664
Fragments arranged by size:
2664 1657 835 32 32 30 30 2
HincII GTy'rAC
Cuts at: 207 375 2321 207
Size: 168 1946 3168
Fragments arranged by size:
3168 1946 168
KasI G'GCGC_C
Cuts at: 403 2712 2960 403
Size: 2309 248 2725
Fragments arranged by size:
2725 2309 248
KpnI G_GTAC'C
Cuts at: 2302 2302
Size: 5282
MluI A'CGCG_T
Cuts at: 4715 4715
Size: 5282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3953 4137 3953
Size: 184 5098
MscI TGG'CCA
Cuts at: 550 1888 3043 550
Size: 1338 1155 2789
Fragments arranged by size:
2789 1338 1155
MslI CAynn'nnrTG
Cuts at: 264 361 1613 3271 3398 264
Size: 97 1252 1658 127 2148
Fragments arranged by size:
2148 1658 1252 127 97
MspA1I CmG'CkG
Cuts at: 245 605 1277 1283 1397 1690 1798 2020 2393
Size: 360 672 6 114 293 108 222 373
Cuts at: 2393 2707 3067 4080 4325 245
Size: 314 360 1013 245 1202
Fragments arranged by size:
1202 1013 672 373 360 360 314 293
245 222 114 108 6
NaeI GCC'GGC
Cuts at: 1057 1407 3463 1057
Size: 350 2056 2876
Fragments arranged by size:
2876 2056 350
NarI GG'CG_CC
Cuts at: 404 2713 2961 404
Size: 2309 248 2725
Fragments arranged by size:
2725 2309 248
NcoI C'CATG_G
Cuts at: 107 900 107
Size: 793 4489
NgoMIV G'CCGG_C
Cuts at: 1055 1405 3461 1055
Size: 350 2056 2876
Fragments arranged by size:
2876 2056 350
NotI GC'GGCC_GC
Cuts at: 606 606
Size: 5282
NspI r_CATG'y
Cuts at: 2335 2835 3742 2335
Size: 500 907 3875
Fragments arranged by size:
3875 907 500
PasI CC'CwG_GG
Cuts at: 1739 2109 1739
Size: 370 4912
PciI A'CATG_T
Cuts at: 3738 3738
Size: 5282
PmeI GTTT'AAAC
Cuts at: 2281 2281
Size: 5282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1141 1173 1486 1518 4421 4453 1141
Size: 32 313 32 2903 32 1970
Fragments arranged by size:
2903 1970 313 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 591 969 1645 2043 2135 591
Size: 378 676 398 92 3738
Fragments arranged by size:
3738 676 398 378 92
PspOMI G'GGCC_C
Cuts at: 760 1683 760
Size: 923 4359
PstI C_TGCA'G
Cuts at: 197 1336 1543 1829 2329 3014 197
Size: 1139 207 286 500 685 2465
Fragments arranged by size:
2465 1139 685 500 286 207
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5282
PvuII CAG'CTG
Cuts at: 1277 2707 3067 1277
Size: 1430 360 3492
Fragments arranged by size:
3492 1430 360
RsrII CG'GwC_CG
Cuts at: 3477 3477
Size: 5282
SacI G_AGCT'C
Cuts at: 172 2296 172
Size: 2124 3158
SacII CC_GC'GG
Cuts at: 246 246
Size: 5282
SalI G'TCGA_C
Cuts at: 373 2319 373
Size: 1946 3336
SanDI GG'GwC_CC
Cuts at: 1645 2135 1645
Size: 490 4792
SapI GCTCTTCn'nnn_
Cuts at: 1344 3305 3515 1344
Size: 1961 210 3111
Fragments arranged by size:
3111 1961 210
SbfI CC_TGCA'GG
Cuts at: 2329 2329
Size: 5282
ScaI AGT'ACT
Cuts at: 625 4799 625
Size: 4174 1108
SfcI C'TryA_G
Cuts at: 33 193 1332 1539 1825 2325 3010 4003 4194
Size: 160 1139 207 286 500 685 993 191
Cuts at: 4194 33
Size: 1121
Fragments arranged by size:
1139 1121 993 685 500 286 207 191
160
SfoI GGC'GCC
Cuts at: 405 2714 2962 405
Size: 2309 248 2725
Fragments arranged by size:
2725 2309 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5282
SmaI CCC'GGG
Cuts at: 691 1682 2304 691
Size: 991 622 3669
Fragments arranged by size:
3669 991 622
SmlI C'TyrA_G
Cuts at: 298 619 1144 2047 2437 3352 3844 4106 298
Size: 321 525 903 390 915 492 262 1474
Fragments arranged by size:
1474 915 903 525 492 390 321 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5282
SphI G_CATG'C
Cuts at: 2335 2835 2335
Size: 500 4782
StyI C'CwwG_G
Cuts at: 107 900 2416 2770 107
Size: 793 1516 354 2619
Fragments arranged by size:
2619 1516 793 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 568 1731 2865 2998 3457 3666 568
Size: 1163 1134 133 459 209 2184
Fragments arranged by size:
2184 1163 1134 459 209 133
TatI w'GTAC_w
Cuts at: 623 4797 623
Size: 4174 1108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 483 515 724 756 823 855 1410 1442 1527
Size: 32 209 32 67 32 555 32 85
Cuts at: 1527 1559 4799 4831 483
Size: 32 3240 32 934
Fragments arranged by size:
3240 934 555 209 85 67 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3079 3079
Size: 5282
XbaI T'CTAG_A
Cuts at: 2313 2313
Size: 5282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 715 1083 1881 2777 715
Size: 368 798 896 3220
Fragments arranged by size:
3220 896 798 368
XhoI C'TCGA_G
Cuts at: 1144 1144
Size: 5282
XmaI C'CCGG_G
Cuts at: 689 1680 2302 689
Size: 991 622 3669
Fragments arranged by size:
3669 991 622
XmnI GAAnn'nnTTC
Cuts at: 1498 1999 1498
Size: 501 4781
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BbsI
BfrBI BmgBI BmtI BsiWI BsrGI BstEII BstZ17I ClaI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI ZraI