Restriction map for
pF1KE0070
(Circular) (Six-base) MAPSORT of: pF1KE0070.seq Check: 2101 from: 1 to: 4793
pF1KE0070 4793 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 668 668
Size: 4793
AccI GT'mk_AC
Cuts at: 1831 1831
Size: 4793
Acc65I G'GTAC_C
Cuts at: 1809 1809
Size: 4793
AclI AA'CG_TT
Cuts at: 4633 4633
Size: 4793
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 372 643 2618 3050 3797 4065 300
Size: 72 271 1975 432 747 268 1028
Fragments arranged by size:
1975 1028 747 432 271 268 72
AflIII A'CryG_T
Cuts at: 527 1322 2145 3249 4226 527
Size: 795 823 1104 977 1094
Fragments arranged by size:
1104 1094 977 823 795
AgeI A'CCGG_T
Cuts at: 3181 3181
Size: 4793
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1027 1246 1027
Size: 219 4574
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 763 795 763
Size: 32 4761
AlwNI CAG_nnn'CTG
Cuts at: 540 990 1468 2098 3665 540
Size: 450 478 630 1567 1668
Fragments arranged by size:
1668 1567 630 478 450
ApaI G_GGCC'C
Cuts at: 1400 1500 1531 1540 1629 1400
Size: 100 31 9 89 4564
Fragments arranged by size:
4564 100 89 31 9
ApaLI G'TGCA_C
Cuts at: 3563 3563
Size: 4793
ApoI r'AATT_y
Cuts at: 1797 4146 4402 1797
Size: 2349 256 2188
Fragments arranged by size:
2349 2188 256
AseI AT'TA_AT
Cuts at: 20 1765 20
Size: 1745 3048
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4793
AvaI C'yCGr_G
Cuts at: 139 213 694 811 951 1524 1532 1813 139
Size: 74 481 117 140 573 8 281 3119
Fragments arranged by size:
3119 573 481 281 140 117 74 8
AvrII C'CTAG_G
Cuts at: 1425 1425
Size: 4793
BamHI G'GATC_C
Cuts at: 1818 1818
Size: 4793
BanI G'GyrC_C
Cuts at: 382 760 1105 1809 2223 2471 2506 382
Size: 378 345 704 414 248 35 2669
Fragments arranged by size:
2669 704 414 378 345 248 35
BanII G_rGCy'C
Cuts at: 560 950 1081 1130 1335 1400 1451 1500 1531
Size: 390 131 49 205 65 51 49 31
Cuts at: 1531 1540 1629 1782 1807 2837 560
Size: 9 89 153 25 1030 2516
Fragments arranged by size:
2516 1030 390 205 153 131 89 65
51 49 49 31 25 9
BbeI G_GCGC'C
Cuts at: 764 1109 2227 2475 764
Size: 345 1118 248 3082
Fragments arranged by size:
3082 1118 345 248
BbsI GAAGACnn'nnnn_
Cuts at: 183 183
Size: 4793
BbvCI CC'TCA_GC
Cuts at: 112 112
Size: 4793
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4759
BciVI GTATCCnnnnn_n'
Cuts at: 2691 3452 4793 2691
Size: 761 1341 2691
Fragments arranged by size:
2691 1341 761
BclI T'GATC_A
Cuts at: 1709 1718 2313 1709
Size: 9 595 4189
Fragments arranged by size:
4189 595 9
BglI GCCn_nnn'nGGC
Cuts at: 1436 1535 1436
Size: 99 4694
BglII A'GATC_T
Cuts at: 2308 4345 2308
Size: 2037 2756
BlpI GC'TnA_GC
Cuts at: 689 1087 1905 689
Size: 398 818 3577
Fragments arranged by size:
3577 818 398
Bme1580I G_kGCm'C
Cuts at: 1251 1367 1400 1500 1525 1531 1540 1629 2418
Size: 116 33 100 25 6 9 89 789
Cuts at: 2418 2511 3567 1251
Size: 93 1056 2477
Fragments arranged by size:
2477 1056 789 116 100 93 89 33
25 9 6
BmgBI CAC'GTC
Cuts at: 225 1323 225
Size: 1098 3695
BmrI ACTGGGnnnn_n'
Cuts at: 2005 2086 2421 4565 2005
Size: 81 335 2144 2233
Fragments arranged by size:
2233 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 137 3160 137
Size: 3023 1770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 634 1114 1294 1324 499
Size: 135 480 180 30 3968
Fragments arranged by size:
3968 480 180 135 30
Bpu10I CC'TnA_GC
Cuts at: 112 508 1045 112
Size: 396 537 3860
Fragments arranged by size:
3860 537 396
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1969 2848 3340 3638 1969
Size: 879 492 298 3124
Fragments arranged by size:
3124 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1300 4497 1300
Size: 3197 1596
BsaAI yAC'GTr
Cuts at: 2146 2146
Size: 4793
BsaBI GATnn'nnATC
Cuts at: 6 2307 4344 6
Size: 2301 2037 455
Fragments arranged by size:
2301 2037 455
BsaHI Gr'CG_yC
Cuts at: 200 296 761 1106 2224 2472 3174 4670 200
Size: 96 465 345 1118 248 702 1496 323
Fragments arranged by size:
1496 1118 702 465 345 323 248 96
BsaWI w'CCGG_w
Cuts at: 1982 2503 3181 3455 3602 4013 1982
Size: 521 678 274 147 411 2762
Fragments arranged by size:
2762 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 472 502 692 722 763 793 1342 1372 472
Size: 30 190 30 41 30 549 30 3893
Fragments arranged by size:
3893 549 190 41 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1557 1557
Size: 4793
BseYI C'CCAG_C
Cuts at: 155 1007 1587 1628 2218 2976 3553 4049 155
Size: 852 580 41 590 758 577 496 899
Fragments arranged by size:
899 852 758 590 580 577 496 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 1129
Size: 4793
BsiEI CG_ry'CG
Cuts at: 104 825 2335 2381 3589 104
Size: 721 1510 46 1208 1308
Fragments arranged by size:
1510 1308 1208 721 46
BsiHKAI G_wGCw'C
Cuts at: 140 214 476 560 684 950 962 1130 1139
Size: 74 262 84 124 266 12 168 9
Cuts at: 1139 1807 2585 2775 3567 140
Size: 668 778 190 792 1366
Fragments arranged by size:
1366 792 778 668 266 262 190 168
124 84 74 12 9
Bsp1286I G_dGCh'C
Cuts at: 140 214 476 560 684 950 962 1081 1130
Size: 74 262 84 124 266 12 119 49
Cuts at: 1130 1139 1251 1335 1367 1400 1451 1500 1525
Size: 9 112 84 32 33 51 49 25
Cuts at: 1525 1531 1540 1629 1782 1807 2418 2511 2585
Size: 6 9 89 153 25 611 93 74
Cuts at: 2585 2775 2837 3567 140
Size: 190 62 730 1366
Fragments arranged by size:
1366 730 611 266 262 190 153 124
119 112 93 89 84 84 74 74
62 51 49 49 33 32 25 25
12 9 9 6
BspEI T'CCGG_A
Cuts at: 4013 4013
Size: 4793
BspHI T'CATG_A
Cuts at: 344 3969 344
Size: 3625 1168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 557 668 1843 2057 2359 2740 557
Size: 111 1175 214 302 381 2610
Fragments arranged by size:
2610 1175 381 302 214 111
BsrBI CCG'CTC
Cuts at: 50 1491 3085 3139 4623 4788 50
Size: 1441 1594 54 1484 165 55
Fragments arranged by size:
1594 1484 1441 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2705 2705
Size: 4793
BsrFI r'CCGG_y
Cuts at: 824 1480 1632 2791 2972 3181 824
Size: 656 152 1159 181 209 2436
Fragments arranged by size:
2436 1159 656 209 181 152
BssHII G'CGCG_C
Cuts at: 150 229 1091 1233 150
Size: 79 862 142 3710
Fragments arranged by size:
3710 862 142 79
BssSI C'ACGA_G
Cuts at: 1432 3064 3422 1432
Size: 1632 358 2803
Fragments arranged by size:
2803 1632 358
BstAPI GCAn_nnn'nTGC
Cuts at: 273 2170 273
Size: 1897 2896
BstBI TT'CG_AA
Cuts at: 1758 3154 1758
Size: 1396 3397
BstEII G'GTnAC_C
Cuts at: 1635 1635
Size: 4793
BstXI CCAn_nnnn'nTGG
Cuts at: 1120 1120
Size: 4793
BstYI r'GATC_y
Cuts at: 1 1818 2285 2308 2643 2889 3901 3987 3999
Size: 1817 467 23 335 246 1012 86 12
Cuts at: 3999 4345 1
Size: 346 449
Fragments arranged by size:
1817 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 415 415
Size: 4793
BtgI C'CryG_G
Cuts at: 291 1081 1453 1640 291
Size: 790 372 187 3444
Fragments arranged by size:
3444 790 372 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 789 2743 2924 3087 789
Size: 1954 181 163 2495
Fragments arranged by size:
2495 1954 181 163
BtsI GCAGTG_nn'
Cuts at: 214 2036 214
Size: 1822 2971
DraI TTT'AAA
Cuts at: 1792 1792
Size: 4793
DrdI GACnn_nn'nnGTC
Cuts at: 2499 3357 2499
Size: 858 3935
EaeI y'GGCC_r
Cuts at: 1746 2378 2552 2943 2970 4315 1746
Size: 632 174 391 27 1345 2224
Fragments arranged by size:
2224 1345 632 391 174 27
EagI C'GGCC_G
Cuts at: 2378 2378
Size: 4793
EarI CTCTTCn'nnn_
Cuts at: 283 607 967 1258 1439 2816 3026 283
Size: 324 360 291 181 1377 210 2050
Fragments arranged by size:
2050 1377 360 324 291 210 181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 161 357 3001 3311 3457 4603 161
Size: 196 2644 310 146 1146 351
Fragments arranged by size:
2644 1146 351 310 196 146
EcoICRI GAG'CTC
Cuts at: 558 948 1128 1805 558
Size: 390 180 677 3546
Fragments arranged by size:
3546 677 390 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 372 499 634 643 1114 1294 1324 2618
Size: 72 127 135 9 471 180 30 1294
Cuts at: 2618 3050 3797 4065 300
Size: 432 747 268 1028
Fragments arranged by size:
1294 1028 747 471 432 268 180 135
127 72 30 9
EcoNI CCTnn'n_nnAGG
Cuts at: 664 664
Size: 4793
EcoO109I rG'GnC_Cy
Cuts at: 815 978 1116 1353 1396 1397 1465 1497 1528
Size: 163 138 237 43 1 68 32 31
Cuts at: 1528 1536 1932 815
Size: 8 396 3676
Fragments arranged by size:
3676 396 237 163 138 68 43 32
31 8 1
EcoRI G'AATT_C
Cuts at: 1797 1797
Size: 4793
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 207 2812 207
Size: 2605 2188
FspI TGC'GCA
Cuts at: 1015 1461 2574 1015
Size: 446 1113 3234
Fragments arranged by size:
3234 1113 446
HaeII r_GCGC'y
Cuts at: 342 764 1109 1380 2227 2475 3497 4476 342
Size: 422 345 271 847 248 1022 979 659
Fragments arranged by size:
1022 979 847 659 422 345 271 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 692 724 1552 1584 2635 2665 2667 2697 692
Size: 32 828 32 1051 30 2 30 2788
Fragments arranged by size:
2788 1051 828 32 32 30 30 2
HincII GTy'rAC
Cuts at: 546 920 1832 546
Size: 374 912 3507
Fragments arranged by size:
3507 912 374
HpaI GTT'AAC
Cuts at: 546 546
Size: 4793
KasI G'GCGC_C
Cuts at: 760 1105 2223 2471 760
Size: 345 1118 248 3082
Fragments arranged by size:
3082 1118 345 248
KpnI G_GTAC'C
Cuts at: 1813 1813
Size: 4793
MluI A'CGCG_T
Cuts at: 4226 4226
Size: 4793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 377 611 1175 3464 3648 377
Size: 234 564 2289 184 1522
Fragments arranged by size:
2289 1522 564 234 184
MscI TGG'CCA
Cuts at: 1748 2554 1748
Size: 806 3987
MslI CAynn'nnrTG
Cuts at: 2782 2909 2782
Size: 127 4666
MspA1I CmG'CkG
Cuts at: 293 320 939 1011 1083 1302 1555 1904 2218
Size: 27 619 72 72 219 253 349 314
Cuts at: 2218 2578 3591 3836 293
Size: 360 1013 245 1250
Fragments arranged by size:
1250 1013 619 360 349 314 253 245
219 72 72 27
NaeI GCC'GGC
Cuts at: 1482 2974 1482
Size: 1492 3301
NarI GG'CG_CC
Cuts at: 761 1106 2224 2472 761
Size: 345 1118 248 3082
Fragments arranged by size:
3082 1118 345 248
NgoMIV G'CCGG_C
Cuts at: 1480 2972 1480
Size: 1492 3301
NspI r_CATG'y
Cuts at: 531 1846 2346 3253 531
Size: 1315 500 907 2071
Fragments arranged by size:
2071 1315 907 500
PasI CC'CwG_GG
Cuts at: 1468 1616 1468
Size: 148 4645
PciI A'CATG_T
Cuts at: 527 3249 527
Size: 2722 2071
PfoI T'CCnGG_A
Cuts at: 194 476 194
Size: 282 4511
PmeI GTTT'AAAC
Cuts at: 1792 1792
Size: 4793
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 763 795 3932 3964 763
Size: 32 3137 32 1592
Fragments arranged by size:
3137 1592 32 32
PpuMI rG'GwC_Cy
Cuts at: 1353 1353
Size: 4793
PshAI GACnn'nnGTC
Cuts at: 1351 1351
Size: 4793
PspOMI G'GGCC_C
Cuts at: 1396 1496 1527 1536 1625 1396
Size: 100 31 9 89 4564
Fragments arranged by size:
4564 100 89 31 9
PstI C_TGCA'G
Cuts at: 305 338 554 779 986 1840 2525 305
Size: 33 216 225 207 854 685 2573
Fragments arranged by size:
2573 854 685 225 216 207 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4793
PvuII CAG'CTG
Cuts at: 1011 1302 2218 2578 1011
Size: 291 916 360 3226
Fragments arranged by size:
3226 916 360 291
RsrII CG'GwC_CG
Cuts at: 2988 2988
Size: 4793
SacI G_AGCT'C
Cuts at: 560 950 1130 1807 560
Size: 390 180 677 3546
Fragments arranged by size:
3546 677 390 180
SacII CC_GC'GG
Cuts at: 294 1084 294
Size: 790 4003
SalI G'TCGA_C
Cuts at: 1830 1830
Size: 4793
SapI GCTCTTCn'nnn_
Cuts at: 283 967 1439 2816 3026 283
Size: 684 472 1377 210 2050
Fragments arranged by size:
2050 1377 684 472 210
SbfI CC_TGCA'GG
Cuts at: 554 986 1840 554
Size: 432 854 3507
Fragments arranged by size:
3507 854 432
ScaI AGT'ACT
Cuts at: 4310 4310
Size: 4793
SfcI C'TryA_G
Cuts at: 33 301 334 550 775 982 1836 2521 3514
Size: 268 33 216 225 207 854 685 993
Cuts at: 3514 3705 33
Size: 191 1121
Fragments arranged by size:
1121 993 854 685 268 225 216 207
191 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 1535 1535
Size: 4793
SfoI GGC'GCC
Cuts at: 762 1107 2225 2473 762
Size: 345 1118 248 3082
Fragments arranged by size:
3082 1118 345 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4793
SmaI CCC'GGG
Cuts at: 813 953 1526 1534 1815 813
Size: 140 573 8 281 3791
Fragments arranged by size:
3791 573 281 140 8
SmlI C'TyrA_G
Cuts at: 694 1948 2863 3355 3617 694
Size: 1254 915 492 262 1870
Fragments arranged by size:
1870 1254 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4793
SphI G_CATG'C
Cuts at: 1846 2346 1846
Size: 500 4293
SrfI GCCC'GGGC
Cuts at: 1526 1526
Size: 4793
StuI AGG'CCT
Cuts at: 248 1430 248
Size: 1182 3611
StyI C'CwwG_G
Cuts at: 665 1119 1425 1592 1782 1927 2281 665
Size: 454 306 167 190 145 354 3177
Fragments arranged by size:
3177 454 354 306 190 167 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1021 1601 1608 2376 2509 2968 3177 1021
Size: 580 7 768 133 459 209 2637
Fragments arranged by size:
2637 768 580 459 209 133 7
TatI w'GTAC_w
Cuts at: 4308 4308
Size: 4793
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 429 461 784 816 4310 4342 429
Size: 32 323 32 3494 32 880
Fragments arranged by size:
3494 880 323 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2590 2590
Size: 4793
XbaI T'CTAG_A
Cuts at: 1824 1824
Size: 4793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2288 2288
Size: 4793
XhoI C'TCGA_G
Cuts at: 694 694
Size: 4793
XmaI C'CCGG_G
Cuts at: 811 951 1524 1532 1813 811
Size: 140 573 8 281 3791
Fragments arranged by size:
3791 573 281 140 8
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI BaeI BfrBI
BmtI BsiWI BsmI BsmBI BsrGI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PsiI
PspXI PsrI SanDI SexAI SgrAI SnaBI SspI SwaI
XmnI ZraI