Restriction map for
pF1KE0069
(Circular) (Six-base) MAPSORT of: pF1KE0069.seq Check: 8303 from: 1 to: 5327
pF1KE0069 5327 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AatII G_ACGT'C
Cuts at: 1625 1625
Size: 5327
AccI GT'mk_AC
Cuts at: 1352 2365 1352
Size: 1013 4314
Acc65I G'GTAC_C
Cuts at: 418 2343 418
Size: 1925 3402
AclI AA'CG_TT
Cuts at: 5167 5167
Size: 5327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 1770 3152 3584 4331 4599 400
Size: 1370 1382 432 747 268 1128
Fragments arranged by size:
1382 1370 1128 747 432 268
AfeI AGC'GCT
Cuts at: 1528 1528
Size: 5327
AflIII A'CryG_T
Cuts at: 2679 3783 4760 2679
Size: 1104 977 3246
Fragments arranged by size:
3246 1104 977
AgeI A'CCGG_T
Cuts at: 3715 3715
Size: 5327
AleI CACnn'nnGTG
Cuts at: 1633 1633
Size: 5327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 190 190
Size: 5327
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1675 1707 1675
Size: 32 5295
AlwNI CAG_nnn'CTG
Cuts at: 849 1011 2049 2632 4199 849
Size: 162 1038 583 1567 1977
Fragments arranged by size:
1977 1567 1038 583 162
ApaI G_GGCC'C
Cuts at: 1929 1929
Size: 5327
ApaLI G'TGCA_C
Cuts at: 1703 4097 1703
Size: 2394 2933
ApoI r'AATT_y
Cuts at: 2331 4680 4936 2331
Size: 2349 256 2722
Fragments arranged by size:
2722 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5327
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5327
AvaI C'yCGr_G
Cuts at: 627 929 1228 1928 2235 2257 2347 627
Size: 302 299 700 307 22 90 3607
Fragments arranged by size:
3607 700 307 302 299 90 22
BamHI G'GATC_C
Cuts at: 1334 2352 1334
Size: 1018 4309
BanI G'GyrC_C
Cuts at: 277 418 752 933 1579 1780 1790 2148 2253
Size: 141 334 181 646 201 10 358 105
Cuts at: 2253 2262 2343 2757 3005 3040 277
Size: 9 81 414 248 35 2564
Fragments arranged by size:
2564 646 414 358 334 248 201 181
141 105 81 35 10 9
BanII G_rGCy'C
Cuts at: 129 457 715 1874 1889 1929 2341 3371 129
Size: 328 258 1159 15 40 412 1030 2085
Fragments arranged by size:
2085 1159 1030 412 328 258 40 15
BbeI G_GCGC'C
Cuts at: 1784 2257 2266 2761 3009 1784
Size: 473 9 495 248 4102
Fragments arranged by size:
4102 495 473 248 9
BbsI GAAGACnn'nnnn_
Cuts at: 829 1402 1448 829
Size: 573 46 4708
Fragments arranged by size:
4708 573 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 141 175 90
Size: 34 17 34 5242
Fragments arranged by size:
5242 34 34 17
BciVI GTATCCnnnnn_n'
Cuts at: 3225 3986 5327 3225
Size: 761 1341 3225
Fragments arranged by size:
3225 1341 761
BclI T'GATC_A
Cuts at: 2847 2847
Size: 5327
BglI GCCn_nnn'nGGC
Cuts at: 319 503 1025 1559 319
Size: 184 522 534 4087
Fragments arranged by size:
4087 534 522 184
BglII A'GATC_T
Cuts at: 2842 4879 2842
Size: 2037 3290
BlpI GC'TnA_GC
Cuts at: 1343 1883 2439 1343
Size: 540 556 4231
Fragments arranged by size:
4231 556 540
Bme1580I G_kGCm'C
Cuts at: 280 536 1109 1584 1667 1707 1918 1929 2952
Size: 256 573 475 83 40 211 11 1023
Cuts at: 2952 3045 4101 280
Size: 93 1056 1506
Fragments arranged by size:
1506 1056 1023 573 475 256 211 93
83 40 11
BmgBI CAC'GTC
Cuts at: 777 777
Size: 5327
BmrI ACTGGGnnnn_n'
Cuts at: 1938 2539 2620 2955 5099 1938
Size: 601 81 335 2144 2166
Fragments arranged by size:
2166 2144 601 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 143 3694 143
Size: 3551 1776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 826 739
Size: 87 5240
Bpu10I CC'TnA_GC
Cuts at: 1888 1888
Size: 5327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1264 2503 3382 3874 4172 1264
Size: 1239 879 492 298 2419
Fragments arranged by size:
2419 1239 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 634 5031 634
Size: 4397 930
BsaAI yAC'GTr
Cuts at: 1464 2199 2680 1464
Size: 735 481 4111
Fragments arranged by size:
4111 735 481
BsaBI GATnn'nnATC
Cuts at: 6 2841 4878 6
Size: 2835 2037 455
Fragments arranged by size:
2835 2037 455
BsaHI Gr'CG_yC
Cuts at: 326 1622 1781 2254 2263 2758 3006 3708 5204
Size: 1296 159 473 9 495 248 702 1496
Cuts at: 5204 326
Size: 449
Fragments arranged by size:
1496 1296 702 495 473 449 248 159
9
BsaWI w'CCGG_w
Cuts at: 749 2516 3037 3715 3989 4136 4547 749
Size: 1767 521 678 274 147 411 1529
Fragments arranged by size:
1767 1529 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 163 193 1675 1705 163
Size: 30 1482 30 3785
Fragments arranged by size:
3785 1482 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 359 465 1683 2085 253
Size: 106 106 1218 402 3495
Fragments arranged by size:
3495 1218 402 106 106
BseYI C'CCAG_C
Cuts at: 1137 1562 2184 2752 3510 4087 4583 1137
Size: 425 622 568 758 577 496 1881
Fragments arranged by size:
1881 758 622 577 568 496 425
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 1138
Size: 5327
BsiEI CG_ry'CG
Cuts at: 104 561 2869 2915 4123 104
Size: 457 2308 46 1208 1308
Fragments arranged by size:
2308 1308 1208 457 46
BsiHKAI G_wGCw'C
Cuts at: 161 374 457 1220 1430 1707 1889 2236 2341
Size: 213 83 763 210 277 182 347 105
Cuts at: 2341 3119 3309 4101 161
Size: 778 190 792 1387
Fragments arranged by size:
1387 792 778 763 347 277 213 210
190 182 105 83
BsiWI C'GTAC_G
Cuts at: 1400 1400
Size: 5327
BsmI GAATG_Cn'
Cuts at: 1809 1809
Size: 5327
BsmBI CGTCTCn'nnnn_
Cuts at: 1473 2051 2228 1473
Size: 578 177 4572
Fragments arranged by size:
4572 578 177
Bsp1286I G_dGCh'C
Cuts at: 129 161 280 374 457 536 715 1109 1220
Size: 32 119 94 83 79 179 394 111
Cuts at: 1220 1430 1584 1667 1707 1874 1889 1918 1929
Size: 210 154 83 40 167 15 29 11
Cuts at: 1929 2236 2341 2952 3045 3119 3309 3371 4101
Size: 307 105 611 93 74 190 62 730
Cuts at: 4101 129
Size: 1355
Fragments arranged by size:
1355 730 611 394 307 210 190 179
167 154 119 111 105 94 93 83
83 79 74 62 40 32 29 15
11
BspEI T'CCGG_A
Cuts at: 4547 4547
Size: 5327
BspHI T'CATG_A
Cuts at: 4503 4503
Size: 5327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1160 1211 1338 2126 2377 2591 2893 3274 1160
Size: 51 127 788 251 214 302 381 3213
Fragments arranged by size:
3213 788 381 302 251 214 127 51
BsrBI CCG'CTC
Cuts at: 50 122 557 1332 1761 3619 3673 5157 5322
Size: 72 435 775 429 1858 54 1484 165
Cuts at: 5322 50
Size: 55
Fragments arranged by size:
1858 1484 775 435 429 165 72 55
54
BsrDI GCAATG_nn'
Cuts at: 3239 3239
Size: 5327
BsrFI r'CCGG_y
Cuts at: 384 1187 1268 1540 3325 3506 3715 384
Size: 803 81 272 1785 181 209 1996
Fragments arranged by size:
1996 1785 803 272 209 181 81
BssSI C'ACGA_G
Cuts at: 1021 3598 3956 1021
Size: 2577 358 2392
Fragments arranged by size:
2577 2392 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2704 2704
Size: 5327
BstBI TT'CG_AA
Cuts at: 3688 3688
Size: 5327
BstXI CCAn_nnnn'nTGG
Cuts at: 336 1243 336
Size: 907 4420
BstYI r'GATC_y
Cuts at: 1 1071 1232 1289 1334 2352 2819 2842 3177
Size: 1070 161 57 45 1018 467 23 335
Cuts at: 3177 3423 4435 4521 4533 4879 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1070 1018 1012 467 449 346 335 246
161 86 57 45 23 12
BtgI C'CryG_G
Cuts at: 434 536 947 1256 1472 1574 1776 2175 434
Size: 102 411 309 216 102 202 399 3586
Fragments arranged by size:
3586 411 399 309 216 202 102 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 291 596 1095 3277 3458 3621 291
Size: 305 499 2182 181 163 1997
Fragments arranged by size:
2182 1997 499 305 181 163
BtsI GCAGTG_nn'
Cuts at: 291 922 1320 1640 2236 2570 291
Size: 631 398 320 596 334 3048
Fragments arranged by size:
3048 631 596 398 334 320
DraI TTT'AAA
Cuts at: 2326 2326
Size: 5327
DraIII CAC_nnn'GTG
Cuts at: 908 948 1986 908
Size: 40 1038 4249
Fragments arranged by size:
4249 1038 40
DrdI GACnn_nn'nnGTC
Cuts at: 295 1621 3033 3891 295
Size: 1326 1412 858 1731
Fragments arranged by size:
1731 1412 1326 858
EaeI y'GGCC_r
Cuts at: 558 1062 1253 1469 1857 2109 2912 3086 3477
Size: 504 191 216 388 252 803 174 391
Cuts at: 3477 3504 4849 558
Size: 27 1345 1036
Fragments arranged by size:
1345 1036 803 504 391 388 252 216
191 174 27
EagI C'GGCC_G
Cuts at: 558 2912 558
Size: 2354 2973
EarI CTCTTCn'nnn_
Cuts at: 1084 3350 3560 1084
Size: 2266 210 2851
Fragments arranged by size:
2851 2266 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 273 775 1255 3535 3845 3991 5137 273
Size: 502 480 2280 310 146 1146 463
Fragments arranged by size:
2280 1146 502 480 463 310 146
EcoICRI GAG'CTC
Cuts at: 455 1887 2339 455
Size: 1432 452 3443
Fragments arranged by size:
3443 1432 452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 739 826 1770 3152 3584 4331 4599 400
Size: 339 87 944 1382 432 747 268 1128
Fragments arranged by size:
1382 1128 944 747 432 339 268 87
EcoNI CCTnn'n_nnAGG
Cuts at: 1824 1824
Size: 5327
EcoO109I rG'GnC_Cy
Cuts at: 743 846 975 1925 2046 2466 743
Size: 103 129 950 121 420 3604
Fragments arranged by size:
3604 950 420 129 121 103
EcoRI G'AATT_C
Cuts at: 2331 2331
Size: 5327
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 3346 2137
Size: 1209 4118
FspI TGC'GCA
Cuts at: 151 3108 151
Size: 2957 2370
HaeII r_GCGC'y
Cuts at: 1530 1784 2209 2257 2266 2761 3009 4031 5010
Size: 254 425 48 9 495 248 1022 979
Cuts at: 5010 1530
Size: 1847
Fragments arranged by size:
1847 1022 979 495 425 254 248 48
9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 335 367 1078 1110 1586 1618 1876 1908 2208
Size: 32 711 32 476 32 258 32 300
Cuts at: 2208 2240 3169 3199 3201 3231 335
Size: 32 929 30 2 30 2431
Fragments arranged by size:
2431 929 711 476 300 258 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1353 2366 1353
Size: 1013 4314
KasI G'GCGC_C
Cuts at: 1780 2253 2262 2757 3005 1780
Size: 473 9 495 248 4102
Fragments arranged by size:
4102 495 473 248 9
KpnI G_GTAC'C
Cuts at: 422 2347 422
Size: 1925 3402
MluI A'CGCG_T
Cuts at: 4760 4760
Size: 5327
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2123 2136 3998 4182 2123
Size: 13 1862 184 3268
Fragments arranged by size:
3268 1862 184 13
MscI TGG'CCA
Cuts at: 1064 2111 3088 1064
Size: 1047 977 3303
Fragments arranged by size:
3303 1047 977
MslI CAynn'nnrTG
Cuts at: 172 1255 1633 3316 3443 172
Size: 1083 378 1683 127 2056
Fragments arranged by size:
2056 1683 1083 378 127
MspA1I CmG'CkG
Cuts at: 620 740 1275 1658 1778 2177 2245 2277 2438
Size: 120 535 383 120 399 68 32 161
Cuts at: 2438 2752 3112 4125 4370 620
Size: 314 360 1013 245 1577
Fragments arranged by size:
1577 1013 535 399 383 360 314 245
161 120 120 68 32
NaeI GCC'GGC
Cuts at: 1270 3508 1270
Size: 2238 3089
NarI GG'CG_CC
Cuts at: 1781 2254 2263 2758 3006 1781
Size: 473 9 495 248 4102
Fragments arranged by size:
4102 495 473 248 9
NgoMIV G'CCGG_C
Cuts at: 1268 3506 1268
Size: 2238 3089
NotI GC'GGCC_GC
Cuts at: 558 558
Size: 5327
NspI r_CATG'y
Cuts at: 2380 2880 3787 2380
Size: 500 907 3920
Fragments arranged by size:
3920 907 500
PasI CC'CwG_GG
Cuts at: 1874 1874
Size: 5327
PciI A'CATG_T
Cuts at: 3783 3783
Size: 5327
PfoI T'CCnGG_A
Cuts at: 236 236
Size: 5327
PmeI GTTT'AAAC
Cuts at: 2326 2326
Size: 5327
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1675 1707 4466 4498 1675
Size: 32 2759 32 2504
Fragments arranged by size:
2759 2504 32 32
PpuMI rG'GwC_Cy
Cuts at: 2046 2046
Size: 5327
PspOMI G'GGCC_C
Cuts at: 1925 1925
Size: 5327
PstI C_TGCA'G
Cuts at: 676 1560 2374 3059 676
Size: 884 814 685 2944
Fragments arranged by size:
2944 884 814 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5327
PvuII CAG'CTG
Cuts at: 620 1658 2752 3112 620
Size: 1038 1094 360 2835
Fragments arranged by size:
2835 1094 1038 360
RsrII CG'GwC_CG
Cuts at: 3522 3522
Size: 5327
SacI G_AGCT'C
Cuts at: 457 1889 2341 457
Size: 1432 452 3443
Fragments arranged by size:
3443 1432 452
SacII CC_GC'GG
Cuts at: 1779 2178 1779
Size: 399 4928
SalI G'TCGA_C
Cuts at: 1351 2364 1351
Size: 1013 4314
SapI GCTCTTCn'nnn_
Cuts at: 3350 3560 3350
Size: 210 5117
SbfI CC_TGCA'GG
Cuts at: 1560 2374 1560
Size: 814 4513
ScaI AGT'ACT
Cuts at: 4844 4844
Size: 5327
SexAI A'CCwGG_T
Cuts at: 545 545
Size: 5327
SfcI C'TryA_G
Cuts at: 33 672 735 1556 2370 3055 4048 4239 33
Size: 639 63 821 814 685 993 191 1121
Fragments arranged by size:
1121 993 821 814 685 639 191 63
SfoI GGC'GCC
Cuts at: 1782 2255 2264 2759 3007 1782
Size: 473 9 495 248 4102
Fragments arranged by size:
4102 495 473 248 9
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5327
SmaI CCC'GGG
Cuts at: 629 931 2259 2349 629
Size: 302 1328 90 3607
Fragments arranged by size:
3607 1328 302 90
SmlI C'TyrA_G
Cuts at: 1279 2482 3397 3889 4151 1279
Size: 1203 915 492 262 2455
Fragments arranged by size:
2455 1203 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5327
SphI G_CATG'C
Cuts at: 2380 2880 2380
Size: 500 4827
StuI AGG'CCT
Cuts at: 232 1057 232
Size: 825 4502
StyI C'CwwG_G
Cuts at: 1505 1610 2461 2815 1505
Size: 105 851 354 4017
Fragments arranged by size:
4017 851 354 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1567 2910 3043 3502 3711 1567
Size: 1343 133 459 209 3183
Fragments arranged by size:
3183 1343 459 209 133
TatI w'GTAC_w
Cuts at: 585 4842 585
Size: 4257 1070
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 163 195 685 717 4844 4876 163
Size: 32 490 32 4127 32 614
Fragments arranged by size:
4127 614 490 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3124 3124
Size: 5327
XbaI T'CTAG_A
Cuts at: 2358 2358
Size: 5327
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2050 2822 2050
Size: 772 4555
XmaI C'CCGG_G
Cuts at: 627 929 2257 2347 627
Size: 302 1328 90 3607
Fragments arranged by size:
3607 1328 302 90
XmnI GAAnn'nnTTC
Cuts at: 2077 2077
Size: 5327
ZraI GAC'GTC
Cuts at: 1623 1623
Size: 5327
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NotI NspI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AscI AvrII BaeI BbvCI BfrBI
BmtI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI