Restriction map for
pF1KE0066
(Circular) (Six-base) MAPSORT of: pF1KE0066.seq Check: 8301 from: 1 to: 5792
pF1KE0066 5792 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 743 785 800 2830 743
Size: 42 15 2030 3705
Fragments arranged by size:
3705 2030 42 15
Acc65I G'GTAC_C
Cuts at: 916 2808 916
Size: 1892 3900
AclI AA'CG_TT
Cuts at: 5632 5632
Size: 5792
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 414 811 1198 1891 2495 2735 3617 4049 4796
Size: 397 387 693 604 240 882 432 747
Cuts at: 4796 5064 414
Size: 268 1142
Fragments arranged by size:
1142 882 747 693 604 432 397 387
268 240
AfeI AGC'GCT
Cuts at: 1924 1924
Size: 5792
AflII C'TTAA_G
Cuts at: 1234 1234
Size: 5792
AflIII A'CryG_T
Cuts at: 635 2547 2549 3144 4248 5225 635
Size: 1912 2 595 1104 977 1202
Fragments arranged by size:
1912 1202 1104 977 595 2
AgeI A'CCGG_T
Cuts at: 4180 4180
Size: 5792
AlwNI CAG_nnn'CTG
Cuts at: 598 2110 2211 2328 3097 4664 598
Size: 1512 101 117 769 1567 1726
Fragments arranged by size:
1726 1567 1512 769 117 101
ApaI G_GGCC'C
Cuts at: 277 277
Size: 5792
ApaLI G'TGCA_C
Cuts at: 4562 4562
Size: 5792
ApoI r'AATT_y
Cuts at: 2796 5145 5401 2796
Size: 2349 256 3187
Fragments arranged by size:
3187 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5792
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5792
AvaI C'yCGr_G
Cuts at: 122 190 263 325 352 1941 2812 122
Size: 68 73 62 27 1589 871 3102
Fragments arranged by size:
3102 1589 871 73 68 62 27
BamHI G'GATC_C
Cuts at: 2817 2817
Size: 5792
BanI G'GyrC_C
Cuts at: 259 286 312 916 2808 3222 3470 3505 259
Size: 27 26 604 1892 414 248 35 2546
Fragments arranged by size:
2546 1892 604 414 248 35 27 26
BanII G_rGCy'C
Cuts at: 277 425 1397 2684 2806 3836 277
Size: 148 972 1287 122 1030 2233
Fragments arranged by size:
2233 1287 1030 972 148 122
BbeI G_GCGC'C
Cuts at: 263 290 316 3226 3474 263
Size: 27 26 2910 248 2581
Fragments arranged by size:
2910 2581 248 27 26
BbsI GAAGACnn'nnnn_
Cuts at: 533 1412 2483 533
Size: 879 1071 3842
Fragments arranged by size:
3842 1071 879
BbvCI CC'TCA_GC
Cuts at: 1882 2341 1882
Size: 459 5333
BciVI GTATCCnnnnn_n'
Cuts at: 913 1181 3690 4451 5792 913
Size: 268 2509 761 1341 913
Fragments arranged by size:
2509 1341 913 761 268
BclI T'GATC_A
Cuts at: 2580 3312 2580
Size: 732 5060
BglI GCCn_nnn'nGGC
Cuts at: 229 295 2114 229
Size: 66 1819 3907
Fragments arranged by size:
3907 1819 66
BglII A'GATC_T
Cuts at: 3307 5344 3307
Size: 2037 3755
BlpI GC'TnA_GC
Cuts at: 182 337 424 2904 182
Size: 155 87 2480 3070
Fragments arranged by size:
3070 2480 155 87
Bme1580I G_kGCm'C
Cuts at: 277 1727 3417 3510 4566 277
Size: 1450 1690 93 1056 1503
Fragments arranged by size:
1690 1503 1450 1056 93
BmgBI CAC'GTC
Cuts at: 747 747
Size: 5792
BmrI ACTGGGnnnn_n'
Cuts at: 2392 2733 3004 3085 3420 5564 2392
Size: 341 271 81 335 2144 2620
Fragments arranged by size:
2620 2144 341 335 271 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4159 4159
Size: 5792
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1552 1997 2087 2220 1552
Size: 445 90 133 5124
Fragments arranged by size:
5124 445 133 90
Bpu10I CC'TnA_GC
Cuts at: 718 1205 1882 2341 718
Size: 487 677 459 4169
Fragments arranged by size:
4169 677 487 459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1545 2018 2968 3847 4339 4637 472
Size: 1073 473 950 879 492 298 1627
Fragments arranged by size:
1627 1073 950 879 492 473 298
BsaI GGTCTCn'nnnn_
Cuts at: 2105 5496 2105
Size: 3391 2401
BsaAI yAC'GTr
Cuts at: 2550 3145 2550
Size: 595 5197
BsaBI GATnn'nnATC
Cuts at: 6 3306 5343 6
Size: 3300 2037 455
Fragments arranged by size:
3300 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 260 287 313 3223 3471 4173 5669 112
Size: 148 27 26 2910 248 702 1496 235
Fragments arranged by size:
2910 1496 702 248 235 148 27 26
BsaWI w'CCGG_w
Cuts at: 2981 3502 4180 4454 4601 5012 2981
Size: 521 678 274 147 411 3761
Fragments arranged by size:
3761 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 433 547 2223 2637 433
Size: 114 1676 414 3588
Fragments arranged by size:
3588 1676 414 114
BseYI C'CCAG_C
Cuts at: 209 298 408 434 2183 2284 3217 3975 4552
Size: 89 110 26 1749 101 933 758 577
Cuts at: 4552 5048 209
Size: 496 953
Fragments arranged by size:
1749 953 933 758 577 496 110 101
89 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2197 2197
Size: 5792
BsiEI CG_ry'CG
Cuts at: 104 224 3334 3380 4588 104
Size: 120 3110 46 1208 1308
Fragments arranged by size:
3110 1308 1208 120 46
BsiHKAI G_wGCw'C
Cuts at: 208 1397 1988 2806 3584 3774 4566 208
Size: 1189 591 818 778 190 792 1434
Fragments arranged by size:
1434 1189 818 792 778 591 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1832 1832
Size: 5792
Bsp1286I G_dGCh'C
Cuts at: 208 277 425 1397 1727 1988 2684 2806 3417
Size: 69 148 972 330 261 696 122 611
Cuts at: 3417 3510 3584 3774 3836 4566 208
Size: 93 74 190 62 730 1434
Fragments arranged by size:
1434 972 730 696 611 330 261 190
148 122 93 74 69 62
BspEI T'CCGG_A
Cuts at: 5012 5012
Size: 5792
BspHI T'CATG_A
Cuts at: 4968 4968
Size: 5792
BspMI ACCTGCnnnn'nnnn_
Cuts at: 578 2842 3056 3358 3739 578
Size: 2264 214 302 381 2631
Fragments arranged by size:
2631 2264 381 302 214
BsrBI CCG'CTC
Cuts at: 50 257 4084 4138 5622 5787 50
Size: 207 3827 54 1484 165 55
Fragments arranged by size:
3827 1484 207 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1630 2462 3704 1630
Size: 832 1242 3718
Fragments arranged by size:
3718 1242 832
BsrFI r'CCGG_y
Cuts at: 289 2197 3790 3971 4180 289
Size: 1908 1593 181 209 1901
Fragments arranged by size:
1908 1901 1593 209 181
BssHII G'CGCG_C
Cuts at: 509 558 509
Size: 49 5743
BssSI C'ACGA_G
Cuts at: 4063 4421 4063
Size: 358 5434
BstAPI GCAn_nnn'nTGC
Cuts at: 3169 3169
Size: 5792
BstBI TT'CG_AA
Cuts at: 4153 4153
Size: 5792
BstXI CCAn_nnnn'nTGG
Cuts at: 913 2244 913
Size: 1331 4461
BstYI r'GATC_y
Cuts at: 1 1209 2390 2817 3284 3307 3642 3888 4900
Size: 1208 1181 427 467 23 335 246 1012
Cuts at: 4900 4986 4998 5344 1
Size: 86 12 346 449
Fragments arranged by size:
1208 1181 1012 467 449 427 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 786 786
Size: 5792
Bsu36I CC'TnA_GG
Cuts at: 763 988 2192 2204 2774 763
Size: 225 1204 12 570 3781
Fragments arranged by size:
3781 1204 570 225 12
BtgI C'CryG_G
Cuts at: 480 480
Size: 5792
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 265 2274 2298 2794 3742 3923 4086 265
Size: 2009 24 496 948 181 163 1971
Fragments arranged by size:
2009 1971 948 496 181 163 24
BtsI GCAGTG_nn'
Cuts at: 240 2160 3035 240
Size: 1920 875 2997
Fragments arranged by size:
2997 1920 875
DraI TTT'AAA
Cuts at: 2791 2791
Size: 5792
DrdI GACnn_nn'nnGTC
Cuts at: 117 2494 3498 4356 117
Size: 2377 1004 858 1553
Fragments arranged by size:
2377 1553 1004 858
EaeI y'GGCC_r
Cuts at: 221 3377 3551 3942 3969 5314 221
Size: 3156 174 391 27 1345 699
Fragments arranged by size:
3156 1345 699 391 174 27
EagI C'GGCC_G
Cuts at: 221 3377 221
Size: 3156 2636
EarI CTCTTCn'nnn_
Cuts at: 3815 4025 3815
Size: 210 5582
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 142 159 4000 4310 4456 5602 142
Size: 17 3841 310 146 1146 332
Fragments arranged by size:
3841 1146 332 310 146 17
EcoICRI GAG'CTC
Cuts at: 1395 2804 1395
Size: 1409 4383
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 414 811 1198 1552 1891 1997 2087 2220 2495
Size: 397 387 354 339 106 90 133 275
Cuts at: 2495 2735 3617 4049 4796 5064 414
Size: 240 882 432 747 268 1142
Fragments arranged by size:
1142 882 747 432 397 387 354 339
275 268 240 133 106 90
EcoNI CCTnn'n_nnAGG
Cuts at: 1570 1570
Size: 5792
EcoO109I rG'GnC_Cy
Cuts at: 273 492 1818 1875 2107 2931 273
Size: 219 1326 57 232 824 3134
Fragments arranged by size:
3134 1326 824 232 219 57
EcoRI G'AATT_C
Cuts at: 2796 2796
Size: 5792
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1440 1539 2701 3811 1440
Size: 99 1162 1110 3421
Fragments arranged by size:
3421 1162 1110 99
FseI GG_CCGG'CC
Cuts at: 2201 2201
Size: 5792
FspI TGC'GCA
Cuts at: 3573 3573
Size: 5792
HaeII r_GCGC'y
Cuts at: 171 263 290 316 520 1473 1815 1926 3226
Size: 92 27 26 204 953 342 111 1300
Cuts at: 3226 3474 4496 5475 171
Size: 248 1022 979 488
Fragments arranged by size:
1300 1022 979 953 488 342 248 204
111 92 27 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1072 1104 2757 2789 3634 3664 3666 3696 1072
Size: 32 1653 32 845 30 2 30 3168
Fragments arranged by size:
3168 1653 845 32 32 30 30 2
HincII GTy'rAC
Cuts at: 744 2831 744
Size: 2087 3705
HindIII A'AGCT_T
Cuts at: 2428 2586 2428
Size: 158 5634
KasI G'GCGC_C
Cuts at: 259 286 312 3222 3470 259
Size: 27 26 2910 248 2581
Fragments arranged by size:
2910 2581 248 27 26
KpnI G_GTAC'C
Cuts at: 920 2812 920
Size: 1892 3900
MluI A'CGCG_T
Cuts at: 5225 5225
Size: 5792
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 945 4463 4647 945
Size: 3518 184 2090
Fragments arranged by size:
3518 2090 184
MscI TGG'CCA
Cuts at: 3553 3553
Size: 5792
MslI CAynn'nnrTG
Cuts at: 925 1546 1912 1963 3781 3908 925
Size: 621 366 51 1818 127 2809
Fragments arranged by size:
2809 1818 621 366 127 51
MspA1I CmG'CkG
Cuts at: 369 434 482 1890 2349 2397 2903 3217 3577
Size: 65 48 1408 459 48 506 314 360
Cuts at: 3577 4590 4835 369
Size: 1013 245 1326
Fragments arranged by size:
1408 1326 1013 506 459 360 314 245
65 48 48
NaeI GCC'GGC
Cuts at: 291 2199 3973 291
Size: 1908 1774 2110
Fragments arranged by size:
2110 1908 1774
NarI GG'CG_CC
Cuts at: 260 287 313 3223 3471 260
Size: 27 26 2910 248 2581
Fragments arranged by size:
2910 2581 248 27 26
NdeI CA'TA_TG
Cuts at: 2090 2090
Size: 5792
NgoMIV G'CCGG_C
Cuts at: 289 2197 3971 289
Size: 1908 1774 2110
Fragments arranged by size:
2110 1908 1774
NspI r_CATG'y
Cuts at: 639 2449 2505 2845 3345 4252 639
Size: 1810 56 340 500 907 2179
Fragments arranged by size:
2179 1810 907 500 340 56
PasI CC'CwG_GG
Cuts at: 1860 2367 1860
Size: 507 5285
PciI A'CATG_T
Cuts at: 635 4248 635
Size: 3613 2179
PflMI CCAn_nnn'nTGG
Cuts at: 2110 2146 2732 2110
Size: 36 586 5170
Fragments arranged by size:
5170 586 36
PfoI T'CCnGG_A
Cuts at: 932 1371 932
Size: 439 5353
PmeI GTTT'AAAC
Cuts at: 2791 2791
Size: 5792
PmlI CAC'GTG
Cuts at: 2550 2550
Size: 5792
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4931 4963 4931
Size: 32 5760
PpuMI rG'GwC_Cy
Cuts at: 1818 1875 1818
Size: 57 5735
PspOMI G'GGCC_C
Cuts at: 273 273
Size: 5792
PstI C_TGCA'G
Cuts at: 2455 2839 3524 2455
Size: 384 685 4723
Fragments arranged by size:
4723 685 384
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5792
PvuII CAG'CTG
Cuts at: 369 434 1890 2349 2397 3217 3577 369
Size: 65 1456 459 48 820 360 2584
Fragments arranged by size:
2584 1456 820 459 360 65 48
RsrII CG'GwC_CG
Cuts at: 3987 3987
Size: 5792
SacI G_AGCT'C
Cuts at: 1397 2806 1397
Size: 1409 4383
SacII CC_GC'GG
Cuts at: 483 483
Size: 5792
SalI G'TCGA_C
Cuts at: 742 2829 742
Size: 2087 3705
SapI GCTCTTCn'nnn_
Cuts at: 3815 4025 3815
Size: 210 5582
SbfI CC_TGCA'GG
Cuts at: 2839 2839
Size: 5792
ScaI AGT'ACT
Cuts at: 5309 5309
Size: 5792
SfcI C'TryA_G
Cuts at: 33 683 2451 2835 3520 4513 4704 33
Size: 650 1768 384 685 993 191 1121
Fragments arranged by size:
1768 1121 993 685 650 384 191
SfoI GGC'GCC
Cuts at: 261 288 314 3224 3472 261
Size: 27 26 2910 248 2581
Fragments arranged by size:
2910 2581 248 27 26
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5792
SgrAI Cr'CCGG_yG
Cuts at: 289 289
Size: 5792
SmaI CCC'GGG
Cuts at: 124 192 265 327 354 2814 124
Size: 68 73 62 27 2460 3102
Fragments arranged by size:
3102 2460 73 68 62 27
SmlI C'TyrA_G
Cuts at: 487 1234 1524 2033 2947 3862 4354 4616 487
Size: 747 290 509 914 915 492 262 1663
Fragments arranged by size:
1663 915 914 747 509 492 290 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5792
SphI G_CATG'C
Cuts at: 2449 2845 3345 2449
Size: 396 500 4896
Fragments arranged by size:
4896 500 396
SrfI GCCC'GGGC
Cuts at: 354 354
Size: 5792
StuI AGG'CCT
Cuts at: 2010 2772 2010
Size: 762 5030
StyI C'CwwG_G
Cuts at: 1508 2685 2926 3280 1508
Size: 1177 241 354 4020
Fragments arranged by size:
4020 1177 354 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 223 727 2335 3375 3508 3967 4176 223
Size: 504 1608 1040 133 459 209 1839
Fragments arranged by size:
1839 1608 1040 504 459 209 133
TatI w'GTAC_w
Cuts at: 1643 5307 1643
Size: 3664 2128
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 759 791 1840 1872 5309 5341 759
Size: 32 1049 32 3437 32 1210
Fragments arranged by size:
3437 1210 1049 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3589 3589
Size: 5792
XbaI T'CTAG_A
Cuts at: 2823 2823
Size: 5792
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1612 1717 3287 1612
Size: 105 1570 4117
Fragments arranged by size:
4117 1570 105
XmaI C'CCGG_G
Cuts at: 122 190 263 325 352 2812 122
Size: 68 73 62 27 2460 3102
Fragments arranged by size:
3102 2460 73 68 62 27
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsaXI BsiWI BsmI BsrGI
BstEII ClaI CspCI DraIII EcoRV FspAI HpaI MfeI
NcoI NheI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SnaBI SspI SwaI
XhoI XmnI ZraI