Restriction map for
pF1KE0065
(Circular) (Six-base) MAPSORT of: pF1KE0065.seq Check: 5229 from: 1 to: 6203
pF1KE0065 6203 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 1282 3241 1282
Size: 1959 4244
Acc65I G'GTAC_C
Cuts at: 3219 3219
Size: 6203
AclI AA'CG_TT
Cuts at: 6043 6043
Size: 6203
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1367 1634 4028 4460 5207 5475 961
Size: 406 267 2394 432 747 268 1689
Fragments arranged by size:
2394 1689 747 432 406 268 267
AflIII A'CryG_T
Cuts at: 686 3108 3555 4659 5636 686
Size: 2422 447 1104 977 1253
Fragments arranged by size:
2422 1253 1104 977 447
AgeI A'CCGG_T
Cuts at: 4591 4591
Size: 6203
AhdI GACnn_n'nnGTC
Cuts at: 690 1593 690
Size: 903 5300
AlwNI CAG_nnn'CTG
Cuts at: 1152 3508 5075 1152
Size: 2356 1567 2280
Fragments arranged by size:
2356 2280 1567
ApaI G_GGCC'C
Cuts at: 1626 3141 1626
Size: 1515 4688
ApaLI G'TGCA_C
Cuts at: 4973 4973
Size: 6203
ApoI r'AATT_y
Cuts at: 142 235 899 933 1680 2712 2726 3207 5556
Size: 93 664 34 747 1032 14 481 2349
Cuts at: 5556 5812 142
Size: 256 533
Fragments arranged by size:
2349 1032 747 664 533 481 256 93
34 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 6203
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6203
AvaI C'yCGr_G
Cuts at: 1169 2575 3223 1169
Size: 1406 648 4149
Fragments arranged by size:
4149 1406 648
BamHI G'GATC_C
Cuts at: 3228 3228
Size: 6203
BanI G'GyrC_C
Cuts at: 482 2119 2570 3219 3633 3881 3916 482
Size: 1637 451 649 414 248 35 2769
Fragments arranged by size:
2769 1637 649 451 414 248 35
BanII G_rGCy'C
Cuts at: 848 1626 1640 2967 3141 3217 4247 848
Size: 778 14 1327 174 76 1030 2804
Fragments arranged by size:
2804 1327 1030 778 174 76 14
BbeI G_GCGC'C
Cuts at: 2574 3637 3885 2574
Size: 1063 248 4892
Fragments arranged by size:
4892 1063 248
BbsI GAAGACnn'nnnn_
Cuts at: 2847 2847
Size: 6203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1710 1744 2075 2109 1710
Size: 34 331 34 5804
Fragments arranged by size:
5804 331 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4101 4862 6203 4101
Size: 761 1341 4101
Fragments arranged by size:
4101 1341 761
BclI T'GATC_A
Cuts at: 3723 3723
Size: 6203
BglII A'GATC_T
Cuts at: 1126 1293 1423 2089 2582 2589 3718 5755 1126
Size: 167 130 666 493 7 1129 2037 1574
Fragments arranged by size:
2037 1574 1129 666 493 167 130 7
BlpI GC'TnA_GC
Cuts at: 718 1732 3315 718
Size: 1014 1583 3606
Fragments arranged by size:
3606 1583 1014
Bme1580I G_kGCm'C
Cuts at: 1581 1626 3034 3141 3828 3921 4977 1581
Size: 45 1408 107 687 93 1056 2807
Fragments arranged by size:
2807 1408 1056 687 107 93 45
BmgBI CAC'GTC
Cuts at: 1780 2272 1780
Size: 492 5711
BmrI ACTGGGnnnn_n'
Cuts at: 1524 1634 1903 3415 3496 3831 5975 1524
Size: 110 269 1512 81 335 2144 1752
Fragments arranged by size:
2144 1752 1512 335 269 110 81
BmtI G_CTAG'C
Cuts at: 2543 2543
Size: 6203
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2080 2601 4570 2080
Size: 521 1969 3713
Fragments arranged by size:
3713 1969 521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 764 1244 2054 2075 2094 2794 2967 2992 3025
Size: 480 810 21 19 700 173 25 33
Cuts at: 3025 764
Size: 3942
Fragments arranged by size:
3942 810 700 480 173 33 25 21
19
Bpu10I CC'TnA_GC
Cuts at: 2743 2743
Size: 6203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1393 2013 2404 3379 4258 4750 5048 292
Size: 1101 620 391 975 879 492 298 1447
Fragments arranged by size:
1447 1101 975 879 620 492 391 298
BsaI GGTCTCn'nnnn_
Cuts at: 1189 2098 5907 1189
Size: 909 3809 1485
Fragments arranged by size:
3809 1485 909
BsaAI yAC'GTr
Cuts at: 3556 3556
Size: 6203
BsaBI GATnn'nnATC
Cuts at: 6 3717 5754 6
Size: 3711 2037 455
Fragments arranged by size:
3711 2037 455
BsaHI Gr'CG_yC
Cuts at: 2571 3634 3882 4584 6080 2571
Size: 1063 248 702 1496 2694
Fragments arranged by size:
2694 1496 1063 702 248
BsaWI w'CCGG_w
Cuts at: 848 3392 3913 4591 4865 5012 5423 848
Size: 2544 521 678 274 147 411 1628
Fragments arranged by size:
2544 1628 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2229 2259 2229
Size: 30 6173
BseYI C'CCAG_C
Cuts at: 951 1471 1919 2048 3077 3628 4386 4963 5459
Size: 520 448 129 1029 551 758 577 496
Cuts at: 5459 951
Size: 1695
Fragments arranged by size:
1695 1029 758 577 551 520 496 448
129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 1850 1228
Size: 622 5581
BsiEI CG_ry'CG
Cuts at: 104 3745 3791 4999 104
Size: 3641 46 1208 1308
Fragments arranged by size:
3641 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1835 3217 3995 4185 4977 1835
Size: 1382 778 190 792 3061
Fragments arranged by size:
3061 1382 792 778 190
BsmI GAATG_Cn'
Cuts at: 1938 2371 3183 1938
Size: 433 812 4958
Fragments arranged by size:
4958 812 433
Bsp1286I G_dGCh'C
Cuts at: 848 1581 1626 1640 1835 2967 3034 3141 3217
Size: 733 45 14 195 1132 67 107 76
Cuts at: 3217 3828 3921 3995 4185 4247 4977 848
Size: 611 93 74 190 62 730 2074
Fragments arranged by size:
2074 1132 733 730 611 195 190 107
93 76 74 67 62 45 14
BspEI T'CCGG_A
Cuts at: 5423 5423
Size: 6203
BspHI T'CATG_A
Cuts at: 2166 5379 2166
Size: 3213 2990
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3253 3467 3769 4150 3253
Size: 214 302 381 5306
Fragments arranged by size:
5306 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4495 4549 6033 6198 50
Size: 4445 54 1484 165 55
Fragments arranged by size:
4445 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4115 4115
Size: 6203
BsrFI r'CCGG_y
Cuts at: 1093 4201 4382 4591 1093
Size: 3108 181 209 2705
Fragments arranged by size:
3108 2705 209 181
BssSI C'ACGA_G
Cuts at: 1719 4474 4832 1719
Size: 2755 358 3090
Fragments arranged by size:
3090 2755 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3580 3580
Size: 6203
BstBI TT'CG_AA
Cuts at: 4564 4564
Size: 6203
BstEII G'GTnAC_C
Cuts at: 2923 2923
Size: 6203
BstXI CCAn_nnnn'nTGG
Cuts at: 1980 2567 2755 1980
Size: 587 188 5428
Fragments arranged by size:
5428 587 188
BstYI r'GATC_y
Cuts at: 1 149 1126 1293 1423 2089 2582 2589 2673
Size: 148 977 167 130 666 493 7 84
Cuts at: 2673 3228 3695 3718 4053 4299 5311 5397 5409
Size: 555 467 23 335 246 1012 86 12
Cuts at: 5409 5755 1
Size: 346 449
Fragments arranged by size:
1012 977 666 555 493 467 449 346
335 246 167 148 130 86 84 23
12 7
BtgI C'CryG_G
Cuts at: 680 840 1572 2380 680
Size: 160 732 808 4503
Fragments arranged by size:
4503 808 732 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 737 4153 4334 4497 737
Size: 3416 181 163 2443
Fragments arranged by size:
3416 2443 181 163
BtsI GCAGTG_nn'
Cuts at: 1240 1477 2620 3446 1240
Size: 237 1143 826 3997
Fragments arranged by size:
3997 1143 826 237
DraI TTT'AAA
Cuts at: 3202 3202
Size: 6203
DraIII CAC_nnn'GTG
Cuts at: 1585 2948 1585
Size: 1363 4840
DrdI GACnn_nn'nnGTC
Cuts at: 3909 4767 3909
Size: 858 5345
EaeI y'GGCC_r
Cuts at: 3788 3962 4353 4380 5725 3788
Size: 174 391 27 1345 4266
Fragments arranged by size:
4266 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3788 3788
Size: 6203
EarI CTCTTCn'nnn_
Cuts at: 2130 2649 4226 4436 2130
Size: 519 1577 210 3897
Fragments arranged by size:
3897 1577 519 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4411 4721 4867 6013 4411
Size: 310 146 1146 4601
Fragments arranged by size:
4601 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3215 3215
Size: 6203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 764 961 1244 1367 1634 2054 2075 2094 2794
Size: 197 283 123 267 420 21 19 700
Cuts at: 2794 2967 2992 3025 4028 4460 5207 5475 764
Size: 173 25 33 1003 432 747 268 1492
Fragments arranged by size:
1492 1003 747 700 432 420 283 268
267 197 173 123 33 25 21 19
EcoNI CCTnn'n_nnAGG
Cuts at: 1385 1551 2746 1385
Size: 166 1195 4842
Fragments arranged by size:
4842 1195 166
EcoO109I rG'GnC_Cy
Cuts at: 1622 3137 3342 1622
Size: 1515 205 4483
Fragments arranged by size:
4483 1515 205
EcoRI G'AATT_C
Cuts at: 1680 3207 1680
Size: 1527 4676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 381 1067 1124 4222 381
Size: 686 57 3098 2362
Fragments arranged by size:
3098 2362 686 57
FspI TGC'GCA
Cuts at: 2626 3984 2626
Size: 1358 4845
HaeII r_GCGC'y
Cuts at: 665 2574 3637 3885 4907 5886 665
Size: 1909 1063 248 1022 979 982
Fragments arranged by size:
1909 1063 1022 982 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 676 708 1579 1611 2081 2113 2161 2193 2574
Size: 32 871 32 470 32 48 32 381
Cuts at: 2574 2606 4045 4075 4077 4107 676
Size: 32 1439 30 2 30 2772
Fragments arranged by size:
2772 1439 871 470 381 48 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 864 2306 3242 864
Size: 1442 936 3825
Fragments arranged by size:
3825 1442 936
HindIII A'AGCT_T
Cuts at: 814 1200 1989 814
Size: 386 789 5028
Fragments arranged by size:
5028 789 386
HpaI GTT'AAC
Cuts at: 864 864
Size: 6203
KasI G'GCGC_C
Cuts at: 2570 3633 3881 2570
Size: 1063 248 4892
Fragments arranged by size:
4892 1063 248
KpnI G_GTAC'C
Cuts at: 3223 3223
Size: 6203
MluI A'CGCG_T
Cuts at: 5636 5636
Size: 6203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 328 926 2519 4874 5058 328
Size: 598 1593 2355 184 1473
Fragments arranged by size:
2355 1593 1473 598 184
MscI TGG'CCA
Cuts at: 3964 3964
Size: 6203
MslI CAynn'nnrTG
Cuts at: 491 545 2180 3025 3176 4192 4319 491
Size: 54 1635 845 151 1016 127 2375
Fragments arranged by size:
2375 1635 1016 845 151 127 54
MspA1I CmG'CkG
Cuts at: 859 955 2814 3068 3314 3628 3988 5001 5246
Size: 96 1859 254 246 314 360 1013 245
Cuts at: 5246 859
Size: 1816
Fragments arranged by size:
1859 1816 1013 360 314 254 246 245
96
NaeI GCC'GGC
Cuts at: 1095 4384 1095
Size: 3289 2914
NarI GG'CG_CC
Cuts at: 2571 3634 3882 2571
Size: 1063 248 4892
Fragments arranged by size:
4892 1063 248
NcoI C'CATG_G
Cuts at: 840 1572 2380 840
Size: 732 808 4663
Fragments arranged by size:
4663 808 732
NgoMIV G'CCGG_C
Cuts at: 1093 4382 1093
Size: 3289 2914
NheI G'CTAG_C
Cuts at: 2539 2539
Size: 6203
NspI r_CATG'y
Cuts at: 690 3256 3756 4663 690
Size: 2566 500 907 2230
Fragments arranged by size:
2566 2230 907 500
PasI CC'CwG_GG
Cuts at: 1393 1393
Size: 6203
PciI A'CATG_T
Cuts at: 686 4659 686
Size: 3973 2230
PflMI CCAn_nnn'nTGG
Cuts at: 632 2824 632
Size: 2192 4011
PfoI T'CCnGG_A
Cuts at: 741 741
Size: 6203
PmeI GTTT'AAAC
Cuts at: 3202 3202
Size: 6203
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1426 1458 5342 5374 1426
Size: 32 3884 32 2255
Fragments arranged by size:
3884 2255 32 32
PshAI GACnn'nnGTC
Cuts at: 1181 1778 1181
Size: 597 5606
PspOMI G'GGCC_C
Cuts at: 1622 3137 1622
Size: 1515 4688
PstI C_TGCA'G
Cuts at: 1484 2597 3250 3935 1484
Size: 1113 653 685 3752
Fragments arranged by size:
3752 1113 685 653
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6203
PvuII CAG'CTG
Cuts at: 859 955 2814 3068 3628 3988 859
Size: 96 1859 254 560 360 3074
Fragments arranged by size:
3074 1859 560 360 254 96
RsrII CG'GwC_CG
Cuts at: 4398 4398
Size: 6203
SacI G_AGCT'C
Cuts at: 3217 3217
Size: 6203
SalI G'TCGA_C
Cuts at: 3240 3240
Size: 6203
SapI GCTCTTCn'nnn_
Cuts at: 4226 4436 4226
Size: 210 5993
SbfI CC_TGCA'GG
Cuts at: 3250 3250
Size: 6203
ScaI AGT'ACT
Cuts at: 1104 3005 5720 1104
Size: 1901 2715 1587
Fragments arranged by size:
2715 1901 1587
SfcI C'TryA_G
Cuts at: 33 822 1480 1551 2593 3246 3931 4924 5115
Size: 789 658 71 1042 653 685 993 191
Cuts at: 5115 33
Size: 1121
Fragments arranged by size:
1121 1042 993 789 685 658 653 191
71
SfoI GGC'GCC
Cuts at: 2572 3635 3883 2572
Size: 1063 248 4892
Fragments arranged by size:
4892 1063 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6203
SmaI CCC'GGG
Cuts at: 1171 3225 1171
Size: 2054 4149
SmlI C'TyrA_G
Cuts at: 307 1408 1992 2419 3358 4273 4765 5027 307
Size: 1101 584 427 939 915 492 262 1483
Fragments arranged by size:
1483 1101 939 915 584 492 427 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6203
SphI G_CATG'C
Cuts at: 3256 3756 3256
Size: 500 5703
SspI AAT'ATT
Cuts at: 1368 2400 1368
Size: 1032 5171
StuI AGG'CCT
Cuts at: 1163 2752 1163
Size: 1589 4614
StyI C'CwwG_G
Cuts at: 153 840 878 894 1559 1572 1973 2233 2380
Size: 687 38 16 665 13 401 260 147
Cuts at: 2380 3337 3691 153
Size: 957 354 2665
Fragments arranged by size:
2665 957 687 665 401 354 260 147
38 16 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 629 2085 3786 3919 4378 4587 629
Size: 1456 1701 133 459 209 2245
Fragments arranged by size:
2245 1701 1456 459 209 133
TatI w'GTAC_w
Cuts at: 186 362 1102 3003 5718 186
Size: 176 740 1901 2715 671
Fragments arranged by size:
2715 1901 740 671 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 665 697 2650 2682 3029 3061 5720 5752 665
Size: 32 1953 32 347 32 2659 32 1116
Fragments arranged by size:
2659 1953 1116 347 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4000 4000
Size: 6203
XbaI T'CTAG_A
Cuts at: 3234 3234
Size: 6203
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3698 3698
Size: 6203
XmaI C'CCGG_G
Cuts at: 1169 3223 1169
Size: 2054 4149
XmnI GAAnn'nnTTC
Cuts at: 341 817 341
Size: 476 5727
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BseRI BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI MfeI NdeI NotI NruI NsiI PacI PmlI
PpuMI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI