Restriction map for
pF1KE0062
(Circular) (Six-base) MAPSORT of: pF1KE0062.seq Check: 6411 from: 1 to: 5015
pF1KE0062 5015 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 737 1105 2053 737
Size: 368 948 3699
Fragments arranged by size:
3699 948 368
Acc65I G'GTAC_C
Cuts at: 2031 2031
Size: 5015
AclI AA'CG_TT
Cuts at: 4855 4855
Size: 5015
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1862 2840 3272 4019 4287 791
Size: 1071 978 432 747 268 1519
Fragments arranged by size:
1519 1071 978 747 432 268
AflIII A'CryG_T
Cuts at: 362 405 575 1278 2367 3471 4448 362
Size: 43 170 703 1089 1104 977 929
Fragments arranged by size:
1104 1089 977 929 703 170 43
AgeI A'CCGG_T
Cuts at: 3403 3403
Size: 5015
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1898 1930 1898
Size: 32 4983
AlwNI CAG_nnn'CTG
Cuts at: 845 963 2320 3887 845
Size: 118 1357 1567 1973
Fragments arranged by size:
1973 1567 1357 118
ApaLI G'TGCA_C
Cuts at: 3785 3785
Size: 5015
ApoI r'AATT_y
Cuts at: 1333 1956 2019 4368 4624 1333
Size: 623 63 2349 256 1724
Fragments arranged by size:
2349 1724 623 256 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 5015
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5015
AvaI C'yCGr_G
Cuts at: 199 718 2035 199
Size: 519 1317 3179
Fragments arranged by size:
3179 1317 519
BamHI G'GATC_C
Cuts at: 145 1311 2040 145
Size: 1166 729 3120
Fragments arranged by size:
3120 1166 729
BanI G'GyrC_C
Cuts at: 317 2031 2445 2693 2728 317
Size: 1714 414 248 35 2604
Fragments arranged by size:
2604 1714 414 248 35
BanII G_rGCy'C
Cuts at: 731 1645 2029 3059 731
Size: 914 384 1030 2687
Fragments arranged by size:
2687 1030 914 384
BbeI G_GCGC'C
Cuts at: 2449 2697 2449
Size: 248 4767
BbsI GAAGACnn'nnnn_
Cuts at: 468 468
Size: 5015
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1196 1230 1196
Size: 34 4981
BciVI GTATCCnnnnn_n'
Cuts at: 590 2913 3674 5015 590
Size: 2323 761 1341 590
Fragments arranged by size:
2323 1341 761 590
BclI T'GATC_A
Cuts at: 2535 2535
Size: 5015
BglII A'GATC_T
Cuts at: 2530 4567 2530
Size: 2037 2978
BlpI GC'TnA_GC
Cuts at: 2127 2127
Size: 5015
Bme1580I G_kGCm'C
Cuts at: 2640 2733 3789 2640
Size: 93 1056 3866
Fragments arranged by size:
3866 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 734 2227 2308 2643 4787 734
Size: 1493 81 335 2144 962
Fragments arranged by size:
2144 1493 962 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3382 3382
Size: 5015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 308
Size: 5015
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2191 3070 3562 3860 2191
Size: 879 492 298 3346
Fragments arranged by size:
3346 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4719 4719
Size: 5015
BsaAI yAC'GTr
Cuts at: 2368 2368
Size: 5015
BsaBI GATnn'nnATC
Cuts at: 6 536 2529 4566 6
Size: 530 1993 2037 455
Fragments arranged by size:
2037 1993 530 455
BsaHI Gr'CG_yC
Cuts at: 924 2446 2694 3396 4892 924
Size: 1522 248 702 1496 1047
Fragments arranged by size:
1522 1496 1047 702 248
BsaWI w'CCGG_w
Cuts at: 308 2204 2725 3403 3677 3824 4235 308
Size: 1896 521 678 274 147 411 1088
Fragments arranged by size:
1896 1088 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1898 1928 1898
Size: 30 4985
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 254 305 254
Size: 51 4964
BseYI C'CCAG_C
Cuts at: 1995 2440 3198 3775 4271 1995
Size: 445 758 577 496 2739
Fragments arranged by size:
2739 758 577 496 445
BsiEI CG_ry'CG
Cuts at: 104 2557 2603 3811 104
Size: 2453 46 1208 1308
Fragments arranged by size:
2453 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 914 1362 1645 2029 2807 2997 3789 914
Size: 448 283 384 778 190 792 2140
Fragments arranged by size:
2140 792 778 448 384 283 190
Bsp1286I G_dGCh'C
Cuts at: 731 914 1362 1645 2029 2640 2733 2807 2997
Size: 183 448 283 384 611 93 74 190
Cuts at: 2997 3059 3789 731
Size: 62 730 1957
Fragments arranged by size:
1957 730 611 448 384 283 190 183
93 74 62
BspEI T'CCGG_A
Cuts at: 4235 4235
Size: 5015
BspHI T'CATG_A
Cuts at: 627 1320 4191 627
Size: 693 2871 1451
Fragments arranged by size:
2871 1451 693
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2065 2279 2581 2962 2065
Size: 214 302 381 4118
Fragments arranged by size:
4118 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3307 3361 4845 5010 50
Size: 3257 54 1484 165 55
Fragments arranged by size:
3257 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2927 2927
Size: 5015
BsrFI r'CCGG_y
Cuts at: 3013 3194 3403 3013
Size: 181 209 4625
Fragments arranged by size:
4625 209 181
BssSI C'ACGA_G
Cuts at: 3286 3644 3286
Size: 358 4657
BstAPI GCAn_nnn'nTGC
Cuts at: 2392 2392
Size: 5015
BstBI TT'CG_AA
Cuts at: 1737 3376 1737
Size: 1639 3376
BstYI r'GATC_y
Cuts at: 1 145 559 1311 2040 2507 2530 2865 3111
Size: 144 414 752 729 467 23 335 246
Cuts at: 3111 4123 4209 4221 4567 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 752 729 467 449 414 346 335
246 144 86 23 12
BstZ17I GTA'TAC
Cuts at: 738 738
Size: 5015
BtgI C'CryG_G
Cuts at: 1193 1193
Size: 5015
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2965 3146 3309 2965
Size: 181 163 4671
Fragments arranged by size:
4671 181 163
BtsI GCAGTG_nn'
Cuts at: 864 912 2258 864
Size: 48 1346 3621
Fragments arranged by size:
3621 1346 48
ClaI AT'CG_AT
Cuts at: 1427 1427
Size: 5015
DraI TTT'AAA
Cuts at: 2014 2014
Size: 5015
DrdI GACnn_nn'nnGTC
Cuts at: 2721 3579 2721
Size: 858 4157
EaeI y'GGCC_r
Cuts at: 2600 2774 3165 3192 4537 2600
Size: 174 391 27 1345 3078
Fragments arranged by size:
3078 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2600 2600
Size: 5015
EarI CTCTTCn'nnn_
Cuts at: 187 1881 3038 3248 187
Size: 1694 1157 210 1954
Fragments arranged by size:
1954 1694 1157 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3223 3533 3679 4825 3223
Size: 310 146 1146 3413
Fragments arranged by size:
3413 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1643 2027 1643
Size: 384 4631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 791 1862 2840 3272 4019 4287 308
Size: 483 1071 978 432 747 268 1036
Fragments arranged by size:
1071 1036 978 747 483 432 268
EcoO109I rG'GnC_Cy
Cuts at: 2154 2154
Size: 5015
EcoRI G'AATT_C
Cuts at: 2019 2019
Size: 5015
EcoRV GAT'ATC
Cuts at: 1023 1744 1023
Size: 721 4294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1429 3034 1429
Size: 1605 3410
FspI TGC'GCA
Cuts at: 2796 2796
Size: 5015
HaeII r_GCGC'y
Cuts at: 2449 2697 3719 4698 2449
Size: 248 1022 979 2766
Fragments arranged by size:
2766 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1268 1300 2857 2887 2889 2919 1268
Size: 32 1557 30 2 30 3364
Fragments arranged by size:
3364 1557 32 30 30 2
HincII GTy'rAC
Cuts at: 2054 2054
Size: 5015
KasI G'GCGC_C
Cuts at: 2445 2693 2445
Size: 248 4767
KpnI G_GTAC'C
Cuts at: 2035 2035
Size: 5015
MluI A'CGCG_T
Cuts at: 4448 4448
Size: 5015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3686 3870 3686
Size: 184 4831
MscI TGG'CCA
Cuts at: 2776 2776
Size: 5015
MslI CAynn'nnrTG
Cuts at: 626 1469 3004 3131 626
Size: 843 1535 127 2510
Fragments arranged by size:
2510 1535 843 127
MspA1I CmG'CkG
Cuts at: 787 963 1258 1344 2126 2440 2800 3813 4058
Size: 176 295 86 782 314 360 1013 245
Cuts at: 4058 787
Size: 1744
Fragments arranged by size:
1744 1013 782 360 314 295 245 176
86
NaeI GCC'GGC
Cuts at: 3196 3196
Size: 5015
NarI GG'CG_CC
Cuts at: 2446 2694 2446
Size: 248 4767
NcoI C'CATG_G
Cuts at: 1193 1193
Size: 5015
NgoMIV G'CCGG_C
Cuts at: 3194 3194
Size: 5015
NspI r_CATG'y
Cuts at: 366 409 510 549 579 1087 1282 1453 2068
Size: 43 101 39 30 508 195 171 615
Cuts at: 2068 2568 3475 366
Size: 500 907 1906
Fragments arranged by size:
1906 907 615 508 500 195 171 101
43 39 30
PciI A'CATG_T
Cuts at: 362 405 575 1278 3471 362
Size: 43 170 703 2193 1906
Fragments arranged by size:
2193 1906 703 170 43
PflMI CCAn_nnn'nTGG
Cuts at: 963 1852 963
Size: 889 4126
PfoI T'CCnGG_A
Cuts at: 1307 1307
Size: 5015
PmeI GTTT'AAAC
Cuts at: 2014 2014
Size: 5015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1898 1930 4154 4186 1898
Size: 32 2224 32 2727
Fragments arranged by size:
2727 2224 32 32
PspXI vC'TCGA_Gb
Cuts at: 199 199
Size: 5015
PstI C_TGCA'G
Cuts at: 786 2062 2747 786
Size: 1276 685 3054
Fragments arranged by size:
3054 1276 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5015
PvuII CAG'CTG
Cuts at: 787 963 1258 1344 2440 2800 787
Size: 176 295 86 1096 360 3002
Fragments arranged by size:
3002 1096 360 295 176 86
RsrII CG'GwC_CG
Cuts at: 3210 3210
Size: 5015
SacI G_AGCT'C
Cuts at: 1645 2029 1645
Size: 384 4631
SalI G'TCGA_C
Cuts at: 2052 2052
Size: 5015
SapI GCTCTTCn'nnn_
Cuts at: 3038 3248 3038
Size: 210 4805
SbfI CC_TGCA'GG
Cuts at: 2062 2062
Size: 5015
ScaI AGT'ACT
Cuts at: 677 4532 677
Size: 3855 1160
SfcI C'TryA_G
Cuts at: 33 782 2058 2743 3736 3927 33
Size: 749 1276 685 993 191 1121
Fragments arranged by size:
1276 1121 993 749 685 191
SfoI GGC'GCC
Cuts at: 2447 2695 2447
Size: 248 4767
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5015
SmaI CCC'GGG
Cuts at: 2037 2037
Size: 5015
SmlI C'TyrA_G
Cuts at: 199 718 2170 3085 3577 3839 199
Size: 519 1452 915 492 262 1375
Fragments arranged by size:
1452 1375 915 519 492 262
SpeI A'CTAG_T
Cuts at: 67 1828 67
Size: 1761 3254
SphI G_CATG'C
Cuts at: 549 1453 2068 2568 549
Size: 904 615 500 2996
Fragments arranged by size:
2996 904 615 500
SspI AAT'ATT
Cuts at: 664 1296 664
Size: 632 4383
StuI AGG'CCT
Cuts at: 1681 1681
Size: 5015
StyI C'CwwG_G
Cuts at: 974 1193 1676 1748 2006 2149 2503 974
Size: 219 483 72 258 143 354 3486
Fragments arranged by size:
3486 483 354 258 219 143 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2598 2731 3190 3399 2598
Size: 133 459 209 4214
Fragments arranged by size:
4214 459 209 133
TatI w'GTAC_w
Cuts at: 675 4530 675
Size: 3855 1160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4532 4564 4532
Size: 32 4983
Tth111I GACn'n_nGTC
Cuts at: 2812 2812
Size: 5015
XbaI T'CTAG_A
Cuts at: 2046 2046
Size: 5015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2510 2510
Size: 5015
XhoI C'TCGA_G
Cuts at: 199 718 199
Size: 519 4496
XmaI C'CCGG_G
Cuts at: 2035 2035
Size: 5015
XmnI GAAnn'nnTTC
Cuts at: 1376 1376
Size: 5015
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI Bsu36I CspCI DraIII EcoNI FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI ZraI