Restriction map for
pF1KE0060
(Circular) (Six-base) MAPSORT of: pF1KE0060.seq Check: 922 from: 1 to: 4838
pF1KE0060 4838 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1776 1776
Size: 4838
AccI GT'mk_AC
Cuts at: 1876 1876
Size: 4838
Acc65I G'GTAC_C
Cuts at: 1854 1854
Size: 4838
AclI AA'CG_TT
Cuts at: 4678 4678
Size: 4838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1325 1682 2663 3095 3842 4110 1325
Size: 357 981 432 747 268 2053
Fragments arranged by size:
2053 981 747 432 357 268
AflIII A'CryG_T
Cuts at: 774 797 2190 3294 4271 774
Size: 23 1393 1104 977 1341
Fragments arranged by size:
1393 1341 1104 977 23
AgeI A'CCGG_T
Cuts at: 3226 3226
Size: 4838
AlwNI CAG_nnn'CTG
Cuts at: 191 1155 2143 3710 191
Size: 964 988 1567 1319
Fragments arranged by size:
1567 1319 988 964
ApaI G_GGCC'C
Cuts at: 261 261
Size: 4838
ApaLI G'TGCA_C
Cuts at: 3608 3608
Size: 4838
ApoI r'AATT_y
Cuts at: 116 670 1842 4191 4447 116
Size: 554 1172 2349 256 507
Fragments arranged by size:
2349 1172 554 507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4838
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
AvaI C'yCGr_G
Cuts at: 254 885 1858 254
Size: 631 973 3234
Fragments arranged by size:
3234 973 631
AvrII C'CTAG_G
Cuts at: 1171 1171
Size: 4838
BamHI G'GATC_C
Cuts at: 1863 1863
Size: 4838
BanI G'GyrC_C
Cuts at: 1854 2268 2516 2551 1854
Size: 414 248 35 4141
Fragments arranged by size:
4141 414 248 35
BanII G_rGCy'C
Cuts at: 261 1095 1852 2882 261
Size: 834 757 1030 2217
Fragments arranged by size:
2217 1030 834 757
BbeI G_GCGC'C
Cuts at: 2272 2520 2272
Size: 248 4590
BbsI GAAGACnn'nnnn_
Cuts at: 418 846 1655 418
Size: 428 809 3601
Fragments arranged by size:
3601 809 428
BciVI GTATCCnnnnn_n'
Cuts at: 1480 2736 3497 4838 1480
Size: 1256 761 1341 1480
Fragments arranged by size:
1480 1341 1256 761
BclI T'GATC_A
Cuts at: 2358 2358
Size: 4838
BglII A'GATC_T
Cuts at: 1230 2353 4390 1230
Size: 1123 2037 1678
Fragments arranged by size:
2037 1678 1123
BlpI GC'TnA_GC
Cuts at: 1950 1950
Size: 4838
Bme1580I G_kGCm'C
Cuts at: 261 2463 2556 3612 261
Size: 2202 93 1056 1487
Fragments arranged by size:
2202 1487 1056 93
BmgBI CAC'GTC
Cuts at: 777 777
Size: 4838
BmrI ACTGGGnnnn_n'
Cuts at: 637 2050 2131 2466 4610 637
Size: 1413 81 335 2144 865
Fragments arranged by size:
2144 1413 865 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3205 3205
Size: 4838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1202 1515 1761 1202
Size: 313 246 4279
Fragments arranged by size:
4279 313 246
Bpu10I CC'TnA_GC
Cuts at: 1202 1202
Size: 4838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 664 2014 2893 3385 3683 309
Size: 355 1350 879 492 298 1464
Fragments arranged by size:
1464 1350 879 492 355 298
BsaI GGTCTCn'nnnn_
Cuts at: 955 4542 955
Size: 3587 1251
BsaAI yAC'GTr
Cuts at: 2191 2191
Size: 4838
BsaBI GATnn'nnATC
Cuts at: 6 1384 2352 4389 6
Size: 1378 968 2037 455
Fragments arranged by size:
2037 1378 968 455
BsaHI Gr'CG_yC
Cuts at: 2269 2517 3219 4715 2269
Size: 248 702 1496 2392
Fragments arranged by size:
2392 1496 702 248
BsaWI w'CCGG_w
Cuts at: 197 546 1810 2027 2548 3226 3500 3647 4058
Size: 349 1264 217 521 678 274 147 411
Cuts at: 4058 197
Size: 977
Fragments arranged by size:
1264 977 678 521 411 349 274 217
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 902 932 1198 1228 1513 1543 1608 1638 902
Size: 30 266 30 285 30 65 30 4102
Fragments arranged by size:
4102 285 266 65 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 923 1013 436
Size: 487 90 4261
Fragments arranged by size:
4261 487 90
BseYI C'CCAG_C
Cuts at: 162 1333 1362 2263 3021 3598 4094 162
Size: 1171 29 901 758 577 496 906
Fragments arranged by size:
1171 906 901 758 577 496 29
BsiEI CG_ry'CG
Cuts at: 104 2380 2426 3634 104
Size: 2276 46 1208 1308
Fragments arranged by size:
2276 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1852 2630 2820 3612 1852
Size: 778 190 792 3078
Fragments arranged by size:
3078 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 723 723
Size: 4838
Bsp1286I G_dGCh'C
Cuts at: 261 1095 1852 2463 2556 2630 2820 2882 3612
Size: 834 757 611 93 74 190 62 730
Cuts at: 3612 261
Size: 1487
Fragments arranged by size:
1487 834 757 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 197 546 4058 197
Size: 349 3512 977
Fragments arranged by size:
3512 977 349
BspHI T'CATG_A
Cuts at: 4014 4014
Size: 4838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1776 1888 2102 2404 2785 1776
Size: 112 214 302 381 3829
Fragments arranged by size:
3829 381 302 214 112
BsrBI CCG'CTC
Cuts at: 50 239 360 3130 3184 4668 4833 50
Size: 189 121 2770 54 1484 165 55
Fragments arranged by size:
2770 1484 189 165 121 55 54
BsrDI GCAATG_nn'
Cuts at: 1298 1563 1791 2750 1298
Size: 265 228 959 3386
Fragments arranged by size:
3386 959 265 228
BsrFI r'CCGG_y
Cuts at: 345 1618 2836 3017 3226 345
Size: 1273 1218 181 209 1957
Fragments arranged by size:
1957 1273 1218 209 181
BssSI C'ACGA_G
Cuts at: 3109 3467 3109
Size: 358 4480
BstAPI GCAn_nnn'nTGC
Cuts at: 1300 2215 1300
Size: 915 3923
BstBI TT'CG_AA
Cuts at: 3199 3199
Size: 4838
BstEII G'GTnAC_C
Cuts at: 504 504
Size: 4838
BstYI r'GATC_y
Cuts at: 1 1185 1230 1863 2330 2353 2688 2934 3946
Size: 1184 45 633 467 23 335 246 1012
Cuts at: 3946 4032 4044 4390 1
Size: 86 12 346 449
Fragments arranged by size:
1184 1012 633 467 449 346 335 246
86 45 23 12
BtgI C'CryG_G
Cuts at: 914 1480 1565 914
Size: 566 85 4187
Fragments arranged by size:
4187 566 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 504 2788 2969 3132 504
Size: 2284 181 163 2210
Fragments arranged by size:
2284 2210 181 163
BtsI GCAGTG_nn'
Cuts at: 2081 2081
Size: 4838
DraI TTT'AAA
Cuts at: 668 1837 668
Size: 1169 3669
DraIII CAC_nnn'GTG
Cuts at: 579 579
Size: 4838
DrdI GACnn_nn'nnGTC
Cuts at: 223 484 2544 3402 223
Size: 261 2060 858 1659
Fragments arranged by size:
2060 1659 858 261
EaeI y'GGCC_r
Cuts at: 1568 2423 2597 2988 3015 4360 1568
Size: 855 174 391 27 1345 2046
Fragments arranged by size:
2046 1345 855 391 174 27
EagI C'GGCC_G
Cuts at: 2423 2423
Size: 4838
EarI CTCTTCn'nnn_
Cuts at: 129 1497 1701 1717 2861 3071 129
Size: 1368 204 16 1144 210 1896
Fragments arranged by size:
1896 1368 1144 210 204 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3046 3356 3502 4648 3046
Size: 310 146 1146 3236
Fragments arranged by size:
3236 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1850 1850
Size: 4838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1202 1325 1515 1682 1761 2663 3095 3842 4110
Size: 123 190 167 79 902 432 747 268
Cuts at: 4110 1202
Size: 1930
Fragments arranged by size:
1930 902 747 432 268 190 167 123
79
EcoO109I rG'GnC_Cy
Cuts at: 188 477 1748 1977 188
Size: 289 1271 229 3049
Fragments arranged by size:
3049 1271 289 229
EcoRI G'AATT_C
Cuts at: 116 1842 116
Size: 1726 3112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1746 2857 1746
Size: 1111 3727
FspI TGC'GCA
Cuts at: 2619 2619
Size: 4838
HaeII r_GCGC'y
Cuts at: 325 2272 2520 3542 4521 325
Size: 1947 248 1022 979 642
Fragments arranged by size:
1947 1022 979 642 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 573 605 619 651 902 934 2680 2710 2712
Size: 32 14 32 251 32 1746 30 2
Cuts at: 2712 2742 573
Size: 30 2669
Fragments arranged by size:
2669 1746 251 32 32 32 30 30
14 2
HincII GTy'rAC
Cuts at: 752 1430 1877 752
Size: 678 447 3713
Fragments arranged by size:
3713 678 447
KasI G'GCGC_C
Cuts at: 2268 2516 2268
Size: 248 4590
KpnI G_GTAC'C
Cuts at: 1858 1858
Size: 4838
MluI A'CGCG_T
Cuts at: 4271 4271
Size: 4838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 186 308 323 3509 3693 186
Size: 122 15 3186 184 1331
Fragments arranged by size:
3186 1331 184 122 15
MscI TGG'CCA
Cuts at: 1570 2599 1570
Size: 1029 3809
MslI CAynn'nnrTG
Cuts at: 2827 2954 2827
Size: 127 4711
MspA1I CmG'CkG
Cuts at: 1482 1781 1949 2263 2623 3636 3881 1482
Size: 299 168 314 360 1013 245 2439
Fragments arranged by size:
2439 1013 360 314 299 245 168
NaeI GCC'GGC
Cuts at: 3019 3019
Size: 4838
NarI GG'CG_CC
Cuts at: 2269 2517 2269
Size: 248 4590
NcoI C'CATG_G
Cuts at: 914 1565 914
Size: 651 4187
NgoMIV G'CCGG_C
Cuts at: 3017 3017
Size: 4838
NspI r_CATG'y
Cuts at: 801 1891 2391 3298 801
Size: 1090 500 907 2341
Fragments arranged by size:
2341 1090 907 500
PciI A'CATG_T
Cuts at: 797 3294 797
Size: 2497 2341
PflMI CCAn_nnn'nTGG
Cuts at: 348 348
Size: 4838
PfoI T'CCnGG_A
Cuts at: 1179 1179
Size: 4838
PmeI GTTT'AAAC
Cuts at: 1837 1837
Size: 4838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 464 496 3977 4009 464
Size: 32 3481 32 1293
Fragments arranged by size:
3481 1293 32 32
PpuMI rG'GwC_Cy
Cuts at: 188 477 188
Size: 289 4549
PsiI TTA'TAA
Cuts at: 1067 1067
Size: 4838
PspOMI G'GGCC_C
Cuts at: 257 257
Size: 4838
PstI C_TGCA'G
Cuts at: 1160 1230 1885 2570 1160
Size: 70 655 685 3428
Fragments arranged by size:
3428 685 655 70
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4838
PvuII CAG'CTG
Cuts at: 2263 2623 2263
Size: 360 4478
RsrII CG'GwC_CG
Cuts at: 3033 3033
Size: 4838
SacI G_AGCT'C
Cuts at: 1852 1852
Size: 4838
SacII CC_GC'GG
Cuts at: 1483 1483
Size: 4838
SalI G'TCGA_C
Cuts at: 1875 1875
Size: 4838
SapI GCTCTTCn'nnn_
Cuts at: 2861 3071 2861
Size: 210 4628
SbfI CC_TGCA'GG
Cuts at: 1885 1885
Size: 4838
ScaI AGT'ACT
Cuts at: 4355 4355
Size: 4838
SfcI C'TryA_G
Cuts at: 33 450 1156 1226 1314 1551 1881 2566 3559
Size: 417 706 70 88 237 330 685 993
Cuts at: 3559 3750 33
Size: 191 1121
Fragments arranged by size:
1121 993 706 685 417 330 237 191
88 70
SfoI GGC'GCC
Cuts at: 2270 2518 2270
Size: 248 4590
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
SmaI CCC'GGG
Cuts at: 256 1860 256
Size: 1604 3234
SmlI C'TyrA_G
Cuts at: 288 679 1993 2908 3400 3662 288
Size: 391 1314 915 492 262 1464
Fragments arranged by size:
1464 1314 915 492 391 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4838
SphI G_CATG'C
Cuts at: 1891 2391 1891
Size: 500 4338
StyI C'CwwG_G
Cuts at: 718 914 1171 1565 1752 1972 2326 718
Size: 196 257 394 187 220 354 3230
Fragments arranged by size:
3230 394 354 257 220 196 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 171 492 1567 1629 2421 2554 3013 3222 171
Size: 321 1075 62 792 133 459 209 1787
Fragments arranged by size:
1787 1075 792 459 321 209 133 62
TatI w'GTAC_w
Cuts at: 1014 4353 1014
Size: 3339 1499
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1608 1640 4355 4387 1608
Size: 32 2715 32 2059
Fragments arranged by size:
2715 2059 32 32
Tth111I GACn'n_nGTC
Cuts at: 502 643 2635 502
Size: 141 1992 2705
Fragments arranged by size:
2705 1992 141
XbaI T'CTAG_A
Cuts at: 1869 1869
Size: 4838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 961 1572 2333 961
Size: 611 761 3466
Fragments arranged by size:
3466 761 611
XmaI C'CCGG_G
Cuts at: 254 1858 254
Size: 1604 3234
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
BaeI BbvCI BcgI BfrBI BglI BmtI BsgI BsiWI
BsmI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI