Restriction map for
pF1KE0058
(Circular) (Six-base) MAPSORT of: pF1KE0058.seq Check: 940 from: 1 to: 5390
pF1KE0058 5390 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 726 726
Size: 5390
AccI GT'mk_AC
Cuts at: 2428 2428
Size: 5390
Acc65I G'GTAC_C
Cuts at: 2406 2406
Size: 5390
AclI AA'CG_TT
Cuts at: 5230 5230
Size: 5390
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 905 920 1510 3215 3647 4394 4662 905
Size: 15 590 1705 432 747 268 1633
Fragments arranged by size:
1705 1633 747 590 432 268 15
AflIII A'CryG_T
Cuts at: 1637 2742 3846 4823 1637
Size: 1105 1104 977 2204
Fragments arranged by size:
2204 1105 1104 977
AgeI A'CCGG_T
Cuts at: 3778 3778
Size: 5390
AlwNI CAG_nnn'CTG
Cuts at: 457 718 2695 4262 457
Size: 261 1977 1567 1585
Fragments arranged by size:
1977 1585 1567 261
ApaI G_GGCC'C
Cuts at: 2218 2218
Size: 5390
ApaLI G'TGCA_C
Cuts at: 4160 4160
Size: 5390
ApoI r'AATT_y
Cuts at: 447 941 1276 1332 1930 2394 4743 4999 447
Size: 494 335 56 598 464 2349 256 838
Fragments arranged by size:
2349 838 598 494 464 335 256 56
AseI AT'TA_AT
Cuts at: 20 20
Size: 5390
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5390
AvaI C'yCGr_G
Cuts at: 1688 2410 1688
Size: 722 4668
BamHI G'GATC_C
Cuts at: 2415 2415
Size: 5390
BanI G'GyrC_C
Cuts at: 906 2081 2406 2820 3068 3103 906
Size: 1175 325 414 248 35 3193
Fragments arranged by size:
3193 1175 414 325 248 35
BanII G_rGCy'C
Cuts at: 647 1302 2218 2404 3434 647
Size: 655 916 186 1030 2603
Fragments arranged by size:
2603 1030 916 655 186
BbeI G_GCGC'C
Cuts at: 2824 3072 2824
Size: 248 5142
BbsI GAAGACnn'nnnn_
Cuts at: 625 625
Size: 5390
BbvCI CC'TCA_GC
Cuts at: 1967 1967
Size: 5390
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1704 1738 1704
Size: 34 5356
BciVI GTATCCnnnnn_n'
Cuts at: 664 3288 4049 5390 664
Size: 2624 761 1341 664
Fragments arranged by size:
2624 1341 761 664
BclI T'GATC_A
Cuts at: 110 2910 110
Size: 2800 2590
BfrBI ATG'CAT
Cuts at: 1721 1721
Size: 5390
BglII A'GATC_T
Cuts at: 1132 2905 4942 1132
Size: 1773 2037 1580
Fragments arranged by size:
2037 1773 1580
BlpI GC'TnA_GC
Cuts at: 2502 2502
Size: 5390
Bme1580I G_kGCm'C
Cuts at: 909 2218 3015 3108 4164 909
Size: 1309 797 93 1056 2135
Fragments arranged by size:
2135 1309 1056 797 93
BmrI ACTGGGnnnn_n'
Cuts at: 159 623 2602 2683 3018 5162 159
Size: 464 1979 81 335 2144 387
Fragments arranged by size:
2144 1979 464 387 335 81
BmtI G_CTAG'C
Cuts at: 860 860
Size: 5390
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3757 3757
Size: 5390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 527 819 1083 1535 512
Size: 15 292 264 452 4367
Fragments arranged by size:
4367 452 292 264 15
Bpu10I CC'TnA_GC
Cuts at: 1227 1967 1227
Size: 740 4650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1871 2566 3445 3937 4235 1871
Size: 695 879 492 298 3026
Fragments arranged by size:
3026 879 695 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 212 1582 5094 212
Size: 1370 3512 508
Fragments arranged by size:
3512 1370 508
BsaAI yAC'GTr
Cuts at: 2743 2743
Size: 5390
BsaBI GATnn'nnATC
Cuts at: 6 1347 2904 4941 6
Size: 1341 1557 2037 455
Fragments arranged by size:
2037 1557 1341 455
BsaHI Gr'CG_yC
Cuts at: 151 2821 3069 3771 5267 151
Size: 2670 248 702 1496 274
Fragments arranged by size:
2670 1496 702 274 248
BsaWI w'CCGG_w
Cuts at: 480 2579 3100 3778 4052 4199 4610 480
Size: 2099 521 678 274 147 411 1260
Fragments arranged by size:
2099 1260 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 192 222 192
Size: 30 5360
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 776 857 1408 1621 1872 776
Size: 81 551 213 251 4294
Fragments arranged by size:
4294 551 251 213 81
BseYI C'CCAG_C
Cuts at: 1734 2260 2815 3573 4150 4646 1734
Size: 526 555 758 577 496 2478
Fragments arranged by size:
2478 758 577 555 526 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 1472 704
Size: 768 4622
BsiEI CG_ry'CG
Cuts at: 104 411 2932 2978 4186 104
Size: 307 2521 46 1208 1308
Fragments arranged by size:
2521 1308 1208 307 46
BsiHKAI G_wGCw'C
Cuts at: 1409 2404 3182 3372 4164 1409
Size: 995 778 190 792 2635
Fragments arranged by size:
2635 995 792 778 190
BsmI GAATG_Cn'
Cuts at: 690 1163 690
Size: 473 4917
BsmBI CGTCTCn'nnnn_
Cuts at: 746 746
Size: 5390
Bsp1286I G_dGCh'C
Cuts at: 647 909 1302 1409 2218 2404 3015 3108 3182
Size: 262 393 107 809 186 611 93 74
Cuts at: 3182 3372 3434 4164 647
Size: 190 62 730 1873
Fragments arranged by size:
1873 809 730 611 393 262 190 186
107 93 74 62
BspEI T'CCGG_A
Cuts at: 480 4610 480
Size: 4130 1260
BspHI T'CATG_A
Cuts at: 2039 4566 2039
Size: 2527 2863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 726 938 2440 2654 2956 3337 726
Size: 212 1502 214 302 381 2779
Fragments arranged by size:
2779 1502 381 302 214 212
BsrBI CCG'CTC
Cuts at: 50 3682 3736 5220 5385 50
Size: 3632 54 1484 165 55
Fragments arranged by size:
3632 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1568 3302 1568
Size: 1734 3656
BsrFI r'CCGG_y
Cuts at: 1759 3388 3569 3778 1759
Size: 1629 181 209 3371
Fragments arranged by size:
3371 1629 209 181
BsrGI T'GTAC_A
Cuts at: 1151 1151
Size: 5390
BssSI C'ACGA_G
Cuts at: 3661 4019 3661
Size: 358 5032
BstAPI GCAn_nnn'nTGC
Cuts at: 2767 2767
Size: 5390
BstBI TT'CG_AA
Cuts at: 3751 3751
Size: 5390
BstEII G'GTnAC_C
Cuts at: 1521 1521
Size: 5390
BstXI CCAn_nnnn'nTGG
Cuts at: 1000 1559 1000
Size: 559 4831
BstYI r'GATC_y
Cuts at: 1 1132 1348 2415 2882 2905 3240 3486 4498
Size: 1131 216 1067 467 23 335 246 1012
Cuts at: 4498 4584 4596 4942 1
Size: 86 12 346 449
Fragments arranged by size:
1131 1067 1012 467 449 346 335 246
216 86 23 12
BtgI C'CryG_G
Cuts at: 999 999
Size: 5390
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1361 1707 3340 3521 3684 1361
Size: 346 1633 181 163 3067
Fragments arranged by size:
3067 1633 346 181 163
BtsI GCAGTG_nn'
Cuts at: 151 491 2143 2213 2633 151
Size: 340 1652 70 420 2908
Fragments arranged by size:
2908 1652 420 340 70
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 782 817 782
Size: 35 5355
DraI TTT'AAA
Cuts at: 2389 2389
Size: 5390
DraIII CAC_nnn'GTG
Cuts at: 1476 1476
Size: 5390
DrdI GACnn_nn'nnGTC
Cuts at: 3096 3954 3096
Size: 858 4532
EaeI y'GGCC_r
Cuts at: 169 1122 1618 1826 2975 3149 3540 3567 4912
Size: 953 496 208 1149 174 391 27 1345
Cuts at: 4912 169
Size: 647
Fragments arranged by size:
1345 1149 953 647 496 391 208 174
27
EagI C'GGCC_G
Cuts at: 2975 2975
Size: 5390
EarI CTCTTCn'nnn_
Cuts at: 3413 3623 3413
Size: 210 5180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 779 905 3598 3908 4054 5200 779
Size: 126 2693 310 146 1146 969
Fragments arranged by size:
2693 1146 969 310 146 126
EcoICRI GAG'CTC
Cuts at: 2402 2402
Size: 5390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 527 819 905 920 1083 1510 1535 3215
Size: 15 292 86 15 163 427 25 1680
Cuts at: 3215 3647 4394 4662 512
Size: 432 747 268 1240
Fragments arranged by size:
1680 1240 747 432 427 292 268 163
86 25 15 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1588 1588
Size: 5390
EcoO109I rG'GnC_Cy
Cuts at: 349 1438 1546 2215 2529 349
Size: 1089 108 669 314 3210
Fragments arranged by size:
3210 1089 669 314 108
EcoRI G'AATT_C
Cuts at: 447 941 2394 447
Size: 494 1453 3443
Fragments arranged by size:
3443 1453 494
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1082 3409 1082
Size: 2327 3063
FspI TGC'GCA
Cuts at: 3171 3171
Size: 5390
HaeII r_GCGC'y
Cuts at: 2824 3072 4094 5073 2824
Size: 248 1022 979 3141
Fragments arranged by size:
3141 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 268 300 353 376 385 408 1081
Size: 32 58 32 53 23 9 23 673
Cuts at: 1081 1113 2039 2071 3232 3262 3264 3294 178
Size: 32 926 32 1161 30 2 30 2274
Fragments arranged by size:
2274 1161 926 673 58 53 32 32
32 32 30 30 23 23 9 2
HincII GTy'rAC
Cuts at: 2429 2429
Size: 5390
HindIII A'AGCT_T
Cuts at: 1064 1064
Size: 5390
KasI G'GCGC_C
Cuts at: 2820 3068 2820
Size: 248 5142
KpnI G_GTAC'C
Cuts at: 2410 2410
Size: 5390
MluI A'CGCG_T
Cuts at: 1637 4823 1637
Size: 3186 2204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 326 391 1490 1897 4061 4245 326
Size: 65 1099 407 2164 184 1471
Fragments arranged by size:
2164 1471 1099 407 184 65
MscI TGG'CCA
Cuts at: 171 1124 1620 1828 3151 171
Size: 953 496 208 1323 2410
Fragments arranged by size:
2410 1323 953 496 208
MslI CAynn'nnrTG
Cuts at: 1870 3379 3506 1870
Size: 1509 127 3754
Fragments arranged by size:
3754 1509 127
MspA1I CmG'CkG
Cuts at: 1734 2501 2815 3175 4188 4433 1734
Size: 767 314 360 1013 245 2691
Fragments arranged by size:
2691 1013 767 360 314 245
NaeI GCC'GGC
Cuts at: 3571 3571
Size: 5390
NarI GG'CG_CC
Cuts at: 2821 3069 2821
Size: 248 5142
NcoI C'CATG_G
Cuts at: 999 999
Size: 5390
NgoMIV G'CCGG_C
Cuts at: 3569 3569
Size: 5390
NheI G'CTAG_C
Cuts at: 856 856
Size: 5390
NsiI A_TGCA'T
Cuts at: 1723 1723
Size: 5390
NspI r_CATG'y
Cuts at: 163 1875 2443 2943 3850 163
Size: 1712 568 500 907 1703
Fragments arranged by size:
1712 1703 907 568 500
PasI CC'CwG_GG
Cuts at: 898 898
Size: 5390
PciI A'CATG_T
Cuts at: 3846 3846
Size: 5390
PflMI CCAn_nnn'nTGG
Cuts at: 1476 1476
Size: 5390
PmeI GTTT'AAAC
Cuts at: 2389 2389
Size: 5390
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4529 4561 4529
Size: 32 5358
PpuMI rG'GwC_Cy
Cuts at: 349 1546 349
Size: 1197 4193
PspOMI G'GGCC_C
Cuts at: 2214 2214
Size: 5390
PstI C_TGCA'G
Cuts at: 248 2437 3122 248
Size: 2189 685 2516
Fragments arranged by size:
2516 2189 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5390
PvuII CAG'CTG
Cuts at: 1734 2815 3175 1734
Size: 1081 360 3949
Fragments arranged by size:
3949 1081 360
RsrII CG'GwC_CG
Cuts at: 3585 3585
Size: 5390
SacI G_AGCT'C
Cuts at: 2404 2404
Size: 5390
SalI G'TCGA_C
Cuts at: 2427 2427
Size: 5390
SapI GCTCTTCn'nnn_
Cuts at: 3413 3623 3413
Size: 210 5180
SbfI CC_TGCA'GG
Cuts at: 2437 2437
Size: 5390
ScaI AGT'ACT
Cuts at: 1323 4907 1323
Size: 3584 1806
SexAI A'CCwGG_T
Cuts at: 1517 1583 1517
Size: 66 5324
SfcI C'TryA_G
Cuts at: 33 231 244 766 1317 2433 3118 4111 4302
Size: 198 13 522 551 1116 685 993 191
Cuts at: 4302 33
Size: 1121
Fragments arranged by size:
1121 1116 993 685 551 522 198 191
13
SfoI GGC'GCC
Cuts at: 2822 3070 2822
Size: 248 5142
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5390
SmaI CCC'GGG
Cuts at: 2412 2412
Size: 5390
SmlI C'TyrA_G
Cuts at: 1886 2545 3460 3952 4214 1886
Size: 659 915 492 262 3062
Fragments arranged by size:
3062 915 659 492 262
SpeI A'CTAG_T
Cuts at: 67 399 1770 67
Size: 332 1371 3687
Fragments arranged by size:
3687 1371 332
SphI G_CATG'C
Cuts at: 163 2443 2943 163
Size: 2280 500 2610
Fragments arranged by size:
2610 2280 500
StyI C'CwwG_G
Cuts at: 800 999 1542 2524 2878 800
Size: 199 543 982 354 3312
Fragments arranged by size:
3312 982 543 354 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 194 374 1442 1555 2973 3106 3565 3774 194
Size: 180 1068 113 1418 133 459 209 1810
Fragments arranged by size:
1810 1418 1068 459 209 180 133 113
TatI w'GTAC_w
Cuts at: 1151 1321 1370 1953 2094 4905 1151
Size: 170 49 583 141 2811 1636
Fragments arranged by size:
2811 1636 583 170 141 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 192 224 960 992 1538 1570 2135 2167 2168
Size: 32 736 32 546 32 565 32 1
Cuts at: 2168 2200 4907 4939 192
Size: 32 2707 32 643
Fragments arranged by size:
2707 736 643 565 546 32 32 32
32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 3187 3187
Size: 5390
XbaI T'CTAG_A
Cuts at: 2421 2421
Size: 5390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1473 2885 1473
Size: 1412 3978
XmaI C'CCGG_G
Cuts at: 2410 2410
Size: 5390
XmnI GAAnn'nnTTC
Cuts at: 1066 2024 1066
Size: 958 4432
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BglI BmgBI BsiWI BssHII BstZ17I Bsu36I
ClaI EcoRV FseI FspAI HpaI MfeI NdeI NotI
NruI PacI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI