Restriction map for
pF1KE0056
(Circular) (Six-base) MAPSORT of: pF1KE0056.seq Check: 8132 from: 1 to: 4529
pF1KE0056 4529 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 275 436 275
Size: 161 4368
AccI GT'mk_AC
Cuts at: 1567 1567
Size: 4529
Acc65I G'GTAC_C
Cuts at: 1545 1545
Size: 4529
AclI AA'CG_TT
Cuts at: 4369 4369
Size: 4529
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 939 2354 2786 3533 3801 939
Size: 1415 432 747 268 1667
Fragments arranged by size:
1667 1415 747 432 268
AflIII A'CryG_T
Cuts at: 1881 2985 3962 1881
Size: 1104 977 2448
Fragments arranged by size:
2448 1104 977
AgeI A'CCGG_T
Cuts at: 2917 2917
Size: 4529
AleI CACnn'nnGTG
Cuts at: 437 437
Size: 4529
AlwNI CAG_nnn'CTG
Cuts at: 1085 1834 3401 1085
Size: 749 1567 2213
Fragments arranged by size:
2213 1567 749
ApaLI G'TGCA_C
Cuts at: 3299 3299
Size: 4529
ApoI r'AATT_y
Cuts at: 395 1298 1533 3882 4138 395
Size: 903 235 2349 256 786
Fragments arranged by size:
2349 903 786 256 235
AseI AT'TA_AT
Cuts at: 20 20
Size: 4529
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
AvaI C'yCGr_G
Cuts at: 1549 1549
Size: 4529
BamHI G'GATC_C
Cuts at: 495 1238 1554 495
Size: 743 316 3470
Fragments arranged by size:
3470 743 316
BanI G'GyrC_C
Cuts at: 339 1545 1959 2207 2242 339
Size: 1206 414 248 35 2626
Fragments arranged by size:
2626 1206 414 248 35
BanII G_rGCy'C
Cuts at: 1543 2573 1543
Size: 1030 3499
BbeI G_GCGC'C
Cuts at: 1963 2211 1963
Size: 248 4281
BciVI GTATCCnnnnn_n'
Cuts at: 2427 3188 4529 2427
Size: 761 1341 2427
Fragments arranged by size:
2427 1341 761
BclI T'GATC_A
Cuts at: 2049 2049
Size: 4529
BglII A'GATC_T
Cuts at: 872 2044 4081 872
Size: 1172 2037 1320
Fragments arranged by size:
2037 1320 1172
BlpI GC'TnA_GC
Cuts at: 1641 1641
Size: 4529
Bme1580I G_kGCm'C
Cuts at: 410 2154 2247 3303 410
Size: 1744 93 1056 1636
Fragments arranged by size:
1744 1636 1056 93
BmgBI CAC'GTC
Cuts at: 726 809 726
Size: 83 4446
BmrI ACTGGGnnnn_n'
Cuts at: 1209 1741 1822 2157 4301 1209
Size: 532 81 335 2144 1437
Fragments arranged by size:
2144 1437 532 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1142 649
Size: 493 4036
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1705 2584 3076 3374 1705
Size: 879 492 298 2860
Fragments arranged by size:
2860 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 717 4233 717
Size: 3516 1013
BsaAI yAC'GTr
Cuts at: 708 1882 708
Size: 1174 3355
BsaBI GATnn'nnATC
Cuts at: 6 2043 4080 6
Size: 2037 2037 455
Fragments arranged by size:
2037 2037 455
BsaHI Gr'CG_yC
Cuts at: 752 1960 2208 2910 4406 752
Size: 1208 248 702 1496 875
Fragments arranged by size:
1496 1208 875 702 248
BsaWI w'CCGG_w
Cuts at: 498 1169 1718 2239 2917 3191 3338 3749 498
Size: 671 549 521 678 274 147 411 1278
Fragments arranged by size:
1278 678 671 549 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 361 391 1031 1061 361
Size: 30 640 30 3829
Fragments arranged by size:
3829 640 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 448 448
Size: 4529
BseYI C'CCAG_C
Cuts at: 1954 2712 3289 3785 1954
Size: 758 577 496 2698
Fragments arranged by size:
2698 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 579 462
Size: 117 4412
BsiEI CG_ry'CG
Cuts at: 104 2071 2117 3325 104
Size: 1967 46 1208 1308
Fragments arranged by size:
1967 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 130 416 427 533 1543 2321 2511 3303 130
Size: 286 11 106 1010 778 190 792 1356
Fragments arranged by size:
1356 1010 792 778 286 190 106 11
BsmI GAATG_Cn'
Cuts at: 157 157
Size: 4529
BsmBI CGTCTCn'nnnn_
Cuts at: 681 681
Size: 4529
Bsp1286I G_dGCh'C
Cuts at: 130 410 416 427 533 1543 2154 2247 2321
Size: 280 6 11 106 1010 611 93 74
Cuts at: 2321 2511 2573 3303 130
Size: 190 62 730 1356
Fragments arranged by size:
1356 1010 730 611 280 190 106 93
74 62 11 6
BspEI T'CCGG_A
Cuts at: 3749 3749
Size: 4529
BspHI T'CATG_A
Cuts at: 3705 3705
Size: 4529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 275 436 1579 1793 2095 2476 275
Size: 161 1143 214 302 381 2328
Fragments arranged by size:
2328 1143 381 302 214 161
BsrBI CCG'CTC
Cuts at: 50 2821 2875 4359 4524 50
Size: 2771 54 1484 165 55
Fragments arranged by size:
2771 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 587 2441 587
Size: 1854 2675
BsrFI r'CCGG_y
Cuts at: 568 2527 2708 2917 568
Size: 1959 181 209 2180
Fragments arranged by size:
2180 1959 209 181
BsrGI T'GTAC_A
Cuts at: 973 973
Size: 4529
BssSI C'ACGA_G
Cuts at: 409 2800 3158 409
Size: 2391 358 1780
Fragments arranged by size:
2391 1780 358
BstAPI GCAn_nnn'nTGC
Cuts at: 321 339 1906 321
Size: 18 1567 2944
Fragments arranged by size:
2944 1567 18
BstBI TT'CG_AA
Cuts at: 1029 2890 1029
Size: 1861 2668
BstYI r'GATC_y
Cuts at: 1 383 495 872 1238 1554 2021 2044 2379
Size: 382 112 377 366 316 467 23 335
Cuts at: 2379 2625 3637 3723 3735 4081 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 382 377 366 346 335
316 246 112 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 231 231
Size: 4529
BtgI C'CryG_G
Cuts at: 107 121 218 1328 107
Size: 14 97 1110 3308
Fragments arranged by size:
3308 1110 97 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2479 2660 2823 2479
Size: 181 163 4185
Fragments arranged by size:
4185 181 163
BtsI GCAGTG_nn'
Cuts at: 1772 1772
Size: 4529
DraI TTT'AAA
Cuts at: 393 1528 393
Size: 1135 3394
DraIII CAC_nnn'GTG
Cuts at: 339 420 339
Size: 81 4448
DrdI GACnn_nn'nnGTC
Cuts at: 2235 3093 2235
Size: 858 3671
EaeI y'GGCC_r
Cuts at: 272 960 2114 2288 2679 2706 4051 272
Size: 688 1154 174 391 27 1345 750
Fragments arranged by size:
1345 1154 750 688 391 174 27
EagI C'GGCC_G
Cuts at: 2114 2114
Size: 4529
EarI CTCTTCn'nnn_
Cuts at: 1401 2552 2762 1401
Size: 1151 210 3168
Fragments arranged by size:
3168 1151 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 162 2737 3047 3193 4339 162
Size: 2575 310 146 1146 352
Fragments arranged by size:
2575 1146 352 310 146
EcoICRI GAG'CTC
Cuts at: 1541 1541
Size: 4529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 939 1142 2354 2786 3533 3801 649
Size: 290 203 1212 432 747 268 1377
Fragments arranged by size:
1377 1212 747 432 290 268 203
EcoNI CCTnn'n_nnAGG
Cuts at: 297 297
Size: 4529
EcoO109I rG'GnC_Cy
Cuts at: 227 369 617 624 1668 227
Size: 142 248 7 1044 3088
Fragments arranged by size:
3088 1044 248 142 7
EcoRI G'AATT_C
Cuts at: 1533 1533
Size: 4529
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 4529
FspI TGC'GCA
Cuts at: 2310 2310
Size: 4529
HaeII r_GCGC'y
Cuts at: 1963 2211 3233 4212 1963
Size: 248 1022 979 2280
Fragments arranged by size:
2280 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 361 393 871 903 1447 1479 2371 2401 2403
Size: 32 478 32 544 32 892 30 2
Cuts at: 2403 2433 361
Size: 30 2457
Fragments arranged by size:
2457 892 544 478 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1568 1568
Size: 4529
KasI G'GCGC_C
Cuts at: 1959 2207 1959
Size: 248 4281
KpnI G_GTAC'C
Cuts at: 1549 1549
Size: 4529
MluI A'CGCG_T
Cuts at: 3962 3962
Size: 4529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3200 3384 3200
Size: 184 4345
MscI TGG'CCA
Cuts at: 274 962 2290 274
Size: 688 1328 2513
Fragments arranged by size:
2513 1328 688
MslI CAynn'nnrTG
Cuts at: 437 926 2518 2645 437
Size: 489 1592 127 2321
Fragments arranged by size:
2321 1592 489 127
MspA1I CmG'CkG
Cuts at: 123 1640 1954 2314 3327 3572 123
Size: 1517 314 360 1013 245 1080
Fragments arranged by size:
1517 1080 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2710 2710
Size: 4529
NarI GG'CG_CC
Cuts at: 1960 2208 1960
Size: 248 4281
NcoI C'CATG_G
Cuts at: 107 218 107
Size: 111 4418
NgoMIV G'CCGG_C
Cuts at: 2708 2708
Size: 4529
NruI TCG'CGA
Cuts at: 521 521
Size: 4529
NspI r_CATG'y
Cuts at: 1582 2082 2989 1582
Size: 500 907 3122
Fragments arranged by size:
3122 907 500
PciI A'CATG_T
Cuts at: 2985 2985
Size: 4529
PflMI CCAn_nnn'nTGG
Cuts at: 840 840
Size: 4529
PmeI GTTT'AAAC
Cuts at: 1528 1528
Size: 4529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 4497
PpuMI rG'GwC_Cy
Cuts at: 369 624 369
Size: 255 4274
PstI C_TGCA'G
Cuts at: 1576 2261 1576
Size: 685 3844
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4529
PvuII CAG'CTG
Cuts at: 1954 2314 1954
Size: 360 4169
RsrII CG'GwC_CG
Cuts at: 2724 2724
Size: 4529
SacI G_AGCT'C
Cuts at: 1543 1543
Size: 4529
SacII CC_GC'GG
Cuts at: 124 124
Size: 4529
SalI G'TCGA_C
Cuts at: 1566 1566
Size: 4529
SapI GCTCTTCn'nnn_
Cuts at: 2552 2762 2552
Size: 210 4319
SbfI CC_TGCA'GG
Cuts at: 1576 1576
Size: 4529
ScaI AGT'ACT
Cuts at: 4046 4046
Size: 4529
SexAI A'CCwGG_T
Cuts at: 335 335
Size: 4529
SfcI C'TryA_G
Cuts at: 33 1572 2257 3250 3441 33
Size: 1539 685 993 191 1121
Fragments arranged by size:
1539 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1961 2209 1961
Size: 248 4281
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
SgrAI Cr'CCGG_yG
Cuts at: 568 568
Size: 4529
SmaI CCC'GGG
Cuts at: 1551 1551
Size: 4529
SmlI C'TyrA_G
Cuts at: 1684 2599 3091 3353 1684
Size: 915 492 262 2860
Fragments arranged by size:
2860 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4529
SphI G_CATG'C
Cuts at: 1582 2082 1582
Size: 500 4029
SspI AAT'ATT
Cuts at: 1281 1281
Size: 4529
StuI AGG'CCT
Cuts at: 1368 1368
Size: 4529
StyI C'CwwG_G
Cuts at: 107 218 293 1253 1504 1663 2017 107
Size: 111 75 960 251 159 354 2619
Fragments arranged by size:
2619 960 354 251 159 111 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1191 1426 2112 2245 2704 2913 1191
Size: 235 686 133 459 209 2807
Fragments arranged by size:
2807 686 459 235 209 133
TatI w'GTAC_w
Cuts at: 973 1316 4044 973
Size: 343 2728 1458
Fragments arranged by size:
2728 1458 343
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 784 816 1031 1063 4046 4078 784
Size: 32 215 32 2983 32 1235
Fragments arranged by size:
2983 1235 215 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2326 2326
Size: 4529
XbaI T'CTAG_A
Cuts at: 1560 1560
Size: 4529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2024 2024
Size: 4529
XmaI C'CCGG_G
Cuts at: 1549 1549
Size: 4529
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsiWI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SanDI SfiI SnaBI SrfI SwaI
XhoI XmnI ZraI