Restriction map for
pF1KE0053
(Circular) (Six-base) MAPSORT of: pF1KE0053.seq Check: 7709 from: 1 to: 7817
pF1KE0053 7817 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AatII G_ACGT'C
Cuts at: 1022 4322 1022
Size: 3300 4517
AccI GT'mk_AC
Cuts at: 3407 4174 4855 3407
Size: 767 681 6369
Fragments arranged by size:
6369 767 681
Acc65I G'GTAC_C
Cuts at: 632 4833 632
Size: 4201 3616
AclI AA'CG_TT
Cuts at: 7657 7657
Size: 7817
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 1083 3242 3844 4022 4396 5642 6074 6821
Size: 678 2159 602 178 374 1246 432 747
Cuts at: 6821 7089 405
Size: 268 1133
Fragments arranged by size:
2159 1246 1133 747 678 602 432 374
268 178
AflII C'TTAA_G
Cuts at: 1306 3831 1306
Size: 2525 5292
AflIII A'CryG_T
Cuts at: 5169 6273 7250 5169
Size: 1104 977 5736
Fragments arranged by size:
5736 1104 977
AgeI A'CCGG_T
Cuts at: 6205 6205
Size: 7817
AleI CACnn'nnGTG
Cuts at: 562 655 922 562
Size: 93 267 7457
Fragments arranged by size:
7457 267 93
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 448 562 940 1638 448
Size: 114 378 698 6627
Fragments arranged by size:
6627 698 378 114
AlwNI CAG_nnn'CTG
Cuts at: 765 2017 2213 4315 5122 6689 765
Size: 1252 196 2102 807 1567 1893
Fragments arranged by size:
2102 1893 1567 1252 807 196
ApaLI G'TGCA_C
Cuts at: 1548 6587 1548
Size: 5039 2778
ApoI r'AATT_y
Cuts at: 1326 1454 1631 1851 2266 2439 2693 3691 4029
Size: 128 177 220 415 173 254 998 338
Cuts at: 4029 4612 4648 4676 4821 7170 7426 1326
Size: 583 36 28 145 2349 256 1717
Fragments arranged by size:
2349 1717 998 583 415 338 256 254
220 177 173 145 128 36 28
AseI AT'TA_AT
Cuts at: 20 20
Size: 7817
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7817
AvaI C'yCGr_G
Cuts at: 409 463 2171 4837 409
Size: 54 1708 2666 3389
Fragments arranged by size:
3389 2666 1708 54
BamHI G'GATC_C
Cuts at: 2344 3997 4842 2344
Size: 1653 845 5319
Fragments arranged by size:
5319 1653 845
BanI G'GyrC_C
Cuts at: 129 387 502 556 632 770 1249 1529 4833
Size: 258 115 54 76 138 479 280 3304
Cuts at: 4833 5247 5495 5530 129
Size: 414 248 35 2416
Fragments arranged by size:
3304 2416 479 414 280 258 248 138
115 76 54 35
BanII G_rGCy'C
Cuts at: 221 589 1742 2909 3071 3575 4248 4831 5861
Size: 368 1153 1167 162 504 673 583 1030
Cuts at: 5861 221
Size: 2177
Fragments arranged by size:
2177 1167 1153 1030 673 583 504 368
162
BbeI G_GCGC'C
Cuts at: 133 391 506 774 5251 5499 133
Size: 258 115 268 4477 248 2451
Fragments arranged by size:
4477 2451 268 258 248 115
BbsI GAAGACnn'nnnn_
Cuts at: 2032 2049 2082 3671 4414 2032
Size: 17 33 1589 743 5435
Fragments arranged by size:
5435 1589 743 33 17
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3462 3496 3462
Size: 34 7783
BciVI GTATCCnnnnn_n'
Cuts at: 1963 2320 5715 6476 7817 1963
Size: 357 3395 761 1341 1963
Fragments arranged by size:
3395 1963 1341 761 357
BclI T'GATC_A
Cuts at: 5337 5337
Size: 7817
BfrBI ATG'CAT
Cuts at: 3231 4462 3231
Size: 1231 6586
BglI GCCn_nnn'nGGC
Cuts at: 225 256 225
Size: 31 7786
BglII A'GATC_T
Cuts at: 2808 5332 7369 2808
Size: 2524 2037 3256
Fragments arranged by size:
3256 2524 2037
BlpI GC'TnA_GC
Cuts at: 3098 4929 3098
Size: 1831 5986
Bme1580I G_kGCm'C
Cuts at: 1552 1627 3898 5442 5535 6591 1552
Size: 75 2271 1544 93 1056 2778
Fragments arranged by size:
2778 2271 1544 1056 93 75
BmrI ACTGGGnnnn_n'
Cuts at: 2534 5029 5110 5445 7589 2534
Size: 2495 81 335 2144 2762
Fragments arranged by size:
2762 2495 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 396 1086 6184 396
Size: 690 5098 2029
Fragments arranged by size:
5098 2029 690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 981 1070 1310 1631 2923 343
Size: 638 89 240 321 1292 5237
Fragments arranged by size:
5237 1292 638 321 240 89
Bpu10I CC'TnA_GC
Cuts at: 532 665 532
Size: 133 7684
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 760 3901 4993 5872 6364 6662 712
Size: 48 3141 1092 879 492 298 1867
Fragments arranged by size:
3141 1867 1092 879 492 298 48
BsaI GGTCTCn'nnnn_
Cuts at: 120 384 7521 120
Size: 264 7137 416
Fragments arranged by size:
7137 416 264
BsaAI yAC'GTr
Cuts at: 657 3085 5170 657
Size: 2428 2085 3304
Fragments arranged by size:
3304 2428 2085
BsaBI GATnn'nnATC
Cuts at: 6 2349 5331 7368 6
Size: 2343 2982 2037 455
Fragments arranged by size:
2982 2343 2037 455
BsaHI Gr'CG_yC
Cuts at: 130 388 503 771 815 1019 3242 4283 4319
Size: 258 115 268 44 204 2223 1041 36
Cuts at: 4319 5248 5496 6198 7694 130
Size: 929 248 702 1496 253
Fragments arranged by size:
2223 1496 1041 929 702 268 258 253
248 204 115 44 36
BsaWI w'CCGG_w
Cuts at: 1766 3071 3687 5006 5527 6205 6479 6626 7037
Size: 1305 616 1319 521 678 274 147 411
Cuts at: 7037 1766
Size: 2546
Fragments arranged by size:
2546 1319 1305 678 616 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1443 1473 1553 1583 3916 3946 1443
Size: 30 80 30 2333 30 5314
Fragments arranged by size:
5314 2333 80 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 979 984 1464 2051 3505 979
Size: 5 480 587 1454 5291
Fragments arranged by size:
5291 1454 587 480 5
BseYI C'CCAG_C
Cuts at: 765 892 1139 1435 1902 2892 5242 6000 6577
Size: 127 247 296 467 990 2350 758 577
Cuts at: 6577 7073 765
Size: 496 1509
Fragments arranged by size:
2350 1509 990 758 577 496 467 296
247 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 787 1758 3767 351
Size: 436 971 2009 4401
Fragments arranged by size:
4401 2009 971 436
BsiEI CG_ry'CG
Cuts at: 104 204 232 458 498 596 978 1046 1157
Size: 100 28 226 40 98 382 68 111
Cuts at: 1157 2993 5359 5405 6613 104
Size: 1836 2366 46 1208 1308
Fragments arranged by size:
2366 1836 1308 1208 382 226 111 100
98 68 46 40 28
BsiHKAI G_wGCw'C
Cuts at: 329 919 1552 1742 2909 2960 3071 4248 4831
Size: 590 633 190 1167 51 111 1177 583
Cuts at: 4831 5609 5799 6591 329
Size: 778 190 792 1555
Fragments arranged by size:
1555 1177 1167 792 778 633 590 583
190 190 111 51
BsmI GAATG_Cn'
Cuts at: 1903 3826 4093 4450 1903
Size: 1923 267 357 5270
Fragments arranged by size:
5270 1923 357 267
BsmBI CGTCTCn'nnnn_
Cuts at: 667 3233 3480 667
Size: 2566 247 5004
Fragments arranged by size:
5004 2566 247
Bsp1286I G_dGCh'C
Cuts at: 221 329 589 919 1552 1627 1742 2909 2960
Size: 108 260 330 633 75 115 1167 51
Cuts at: 2960 3071 3575 3898 4248 4831 5442 5535 5609
Size: 111 504 323 350 583 611 93 74
Cuts at: 5609 5799 5861 6591 221
Size: 190 62 730 1447
Fragments arranged by size:
1447 1167 730 633 611 583 504 350
330 323 260 190 115 111 108 93
75 74 62 51
BspEI T'CCGG_A
Cuts at: 3071 3687 7037 3071
Size: 616 3350 3851
Fragments arranged by size:
3851 3350 616
BspHI T'CATG_A
Cuts at: 6993 6993
Size: 7817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1780 3672 4867 5081 5383 5764 1780
Size: 1892 1195 214 302 381 3833
Fragments arranged by size:
3833 1892 1195 381 302 214
BsrBI CCG'CTC
Cuts at: 50 290 872 1065 6109 6163 7647 7812 50
Size: 240 582 193 5044 54 1484 165 55
Fragments arranged by size:
5044 1484 582 240 193 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2357 2858 3383 5729 2357
Size: 501 525 2346 4445
Fragments arranged by size:
4445 2346 525 501
BsrFI r'CCGG_y
Cuts at: 375 457 481 595 883 5815 5996 6205 375
Size: 82 24 114 288 4932 181 209 1987
Fragments arranged by size:
4932 1987 288 209 181 114 82 24
BsrGI T'GTAC_A
Cuts at: 1569 3650 1569
Size: 2081 5736
BssHII G'CGCG_C
Cuts at: 901 1120 1122 901
Size: 219 2 7596
Fragments arranged by size:
7596 219 2
BssSI C'ACGA_G
Cuts at: 6088 6446 6088
Size: 358 7459
BstAPI GCAn_nnn'nTGC
Cuts at: 5194 5194
Size: 7817
BstBI TT'CG_AA
Cuts at: 1644 6178 1644
Size: 4534 3283
BstEII G'GTnAC_C
Cuts at: 3000 3000
Size: 7817
BstXI CCAn_nnnn'nTGG
Cuts at: 1005 2320 1005
Size: 1315 6502
BstYI r'GATC_y
Cuts at: 1 1112 2344 2808 3997 4311 4842 5309 5332
Size: 1111 1232 464 1189 314 531 467 23
Cuts at: 5332 5667 5913 6925 7011 7023 7369 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1232 1189 1111 1012 531 467 464 449
346 335 314 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 695 960 1082 1253 2754 2993 3623 107
Size: 588 265 122 171 1501 239 630 4301
Fragments arranged by size:
4301 1501 630 588 265 239 171 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 237 705 891 5767 5948 6111 237
Size: 468 186 4876 181 163 1943
Fragments arranged by size:
4876 1943 468 186 181 163
BtsI GCAGTG_nn'
Cuts at: 160 1164 1518 3388 4062 5060 160
Size: 1004 354 1870 674 998 2917
Fragments arranged by size:
2917 1870 1004 998 674 354
DraI TTT'AAA
Cuts at: 2546 4816 2546
Size: 2270 5547
DraIII CAC_nnn'GTG
Cuts at: 810 810
Size: 7817
DrdI GACnn_nn'nnGTC
Cuts at: 5523 6381 5523
Size: 858 6959
EaeI y'GGCC_r
Cuts at: 201 455 593 644 716 752 957 963 975
Size: 254 138 51 72 36 205 6 12
Cuts at: 975 1043 1154 1296 2990 3798 3975 5402 5576
Size: 68 111 142 1694 808 177 1427 174
Cuts at: 5576 5967 5994 7339 201
Size: 391 27 1345 679
Fragments arranged by size:
1694 1427 1345 808 679 391 254 205
177 174 142 138 111 72 68 51
36 27 12 6
EagI C'GGCC_G
Cuts at: 201 455 593 975 1043 1154 2990 5402 201
Size: 254 138 382 68 111 1836 2412 2616
Fragments arranged by size:
2616 2412 1836 382 254 138 111 68
EarI CTCTTCn'nnn_
Cuts at: 1102 1364 2279 2340 2755 2874 4358 5840 6050
Size: 262 915 61 415 119 1484 1482 210
Cuts at: 6050 1102
Size: 2869
Fragments arranged by size:
2869 1484 1482 915 415 262 210 119
61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6025 6335 6481 7627 6025
Size: 310 146 1146 6215
Fragments arranged by size:
6215 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1740 2907 3069 4246 4829 1740
Size: 1167 162 1177 583 4728
Fragments arranged by size:
4728 1177 1167 583 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 405 981 1070 1083 1310 1631 2923 3242
Size: 62 576 89 13 227 321 1292 319
Cuts at: 3242 3844 4022 4396 5642 6074 6821 7089 343
Size: 602 178 374 1246 432 747 268 1071
Fragments arranged by size:
1292 1246 1071 747 602 576 432 374
321 319 268 227 178 89 62 13
EcoNI CCTnn'n_nnAGG
Cuts at: 1006 3194 1006
Size: 2188 5629
EcoO109I rG'GnC_Cy
Cuts at: 1011 2634 3619 4956 1011
Size: 1623 985 1337 3872
Fragments arranged by size:
3872 1623 1337 985
EcoRI G'AATT_C
Cuts at: 1454 3691 4821 1454
Size: 2237 1130 4450
Fragments arranged by size:
4450 2237 1130
EcoRV GAT'ATC
Cuts at: 3989 4530 3989
Size: 541 7276
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1568 5836 1568
Size: 4268 3549
FseI GG_CCGG'CC
Cuts at: 461 461
Size: 7817
FspI TGC'GCA
Cuts at: 550 2563 5598 550
Size: 2013 3035 2769
Fragments arranged by size:
3035 2769 2013
FspAI rTGC'GCAy
Cuts at: 2563 2563
Size: 7817
HaeII r_GCGC'y
Cuts at: 133 160 391 506 774 878 1071 1090 5251
Size: 27 231 115 268 104 193 19 4161
Cuts at: 5251 5499 6521 7500 133
Size: 248 1022 979 450
Fragments arranged by size:
4161 1022 979 450 268 248 231 193
115 104 27 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 553 585 1222 1254 1591 1623 2014 2046 2908
Size: 32 637 32 337 32 391 32 862
Cuts at: 2908 2940 3916 3948 5659 5689 5691 5721 553
Size: 32 976 32 1711 30 2 30 2649
Fragments arranged by size:
2649 1711 976 862 637 391 337 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1824 3043 4175 4856 1824
Size: 1219 1132 681 4785
Fragments arranged by size:
4785 1219 1132 681
HindIII A'AGCT_T
Cuts at: 1683 3297 4510 1683
Size: 1614 1213 4990
Fragments arranged by size:
4990 1614 1213
KasI G'GCGC_C
Cuts at: 129 387 502 770 5247 5495 129
Size: 258 115 268 4477 248 2451
Fragments arranged by size:
4477 2451 268 258 248 115
KpnI G_GTAC'C
Cuts at: 636 4837 636
Size: 4201 3616
MluI A'CGCG_T
Cuts at: 7250 7250
Size: 7817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 212 728 1221 1253 3546 3739 6488 6672 212
Size: 516 493 32 2293 193 2749 184 1357
Fragments arranged by size:
2749 2293 1357 516 493 193 184 32
MscI TGG'CCA
Cuts at: 754 1298 3800 3977 5578 754
Size: 544 2502 177 1601 2993
Fragments arranged by size:
2993 2502 1601 544 177
MslI CAynn'nnrTG
Cuts at: 562 655 922 2188 2260 4643 5806 5933 562
Size: 93 267 1266 72 2383 1163 127 2446
Fragments arranged by size:
2446 2383 1266 1163 267 127 93 72
MspA1I CmG'CkG
Cuts at: 296 309 629 686 697 1048 1139 1435 4928
Size: 13 320 57 11 351 91 296 3493
Cuts at: 4928 5242 5602 6615 6860 296
Size: 314 360 1013 245 1253
Fragments arranged by size:
3493 1253 1013 360 351 320 314 296
245 91 57 13 11
NaeI GCC'GGC
Cuts at: 459 597 5998 459
Size: 138 5401 2278
Fragments arranged by size:
5401 2278 138
NarI GG'CG_CC
Cuts at: 130 388 503 771 5248 5496 130
Size: 258 115 268 4477 248 2451
Fragments arranged by size:
4477 2451 268 258 248 115
NcoI C'CATG_G
Cuts at: 107 2754 107
Size: 2647 5170
NgoMIV G'CCGG_C
Cuts at: 457 595 5996 457
Size: 138 5401 2278
Fragments arranged by size:
5401 2278 138
NotI GC'GGCC_GC
Cuts at: 1043 1043
Size: 7817
NsiI A_TGCA'T
Cuts at: 3233 4464 3233
Size: 1231 6586
NspI r_CATG'y
Cuts at: 1910 3468 4771 4870 5370 6277 1910
Size: 1558 1303 99 500 907 3450
Fragments arranged by size:
3450 1558 1303 907 500 99
PasI CC'CwG_GG
Cuts at: 522 522
Size: 7817
PciI A'CATG_T
Cuts at: 6273 6273
Size: 7817
PflMI CCAn_nnn'nTGG
Cuts at: 887 887
Size: 7817
PfoI T'CCnGG_A
Cuts at: 118 3554 3731 118
Size: 3436 177 4204
Fragments arranged by size:
4204 3436 177
PmeI GTTT'AAAC
Cuts at: 4816 4816
Size: 7817
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 913 945 6956 6988 913
Size: 32 6011 32 1742
Fragments arranged by size:
6011 1742 32 32
PpuMI rG'GwC_Cy
Cuts at: 2634 3619 2634
Size: 985 6832
PshAI GACnn'nnGTC
Cuts at: 3359 3359
Size: 7817
PstI C_TGCA'G
Cuts at: 2624 3107 4864 5549 2624
Size: 483 1757 685 4892
Fragments arranged by size:
4892 1757 685 483
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7817
PvuII CAG'CTG
Cuts at: 296 309 1139 1435 5242 5602 296
Size: 13 830 296 3807 360 2511
Fragments arranged by size:
3807 2511 830 360 296 13
RsrII CG'GwC_CG
Cuts at: 6012 6012
Size: 7817
SacI G_AGCT'C
Cuts at: 1742 2909 3071 4248 4831 1742
Size: 1167 162 1177 583 4728
Fragments arranged by size:
4728 1177 1167 583 162
SacII CC_GC'GG
Cuts at: 698 698
Size: 7817
SalI G'TCGA_C
Cuts at: 4173 4854 4173
Size: 681 7136
SanDI GG'GwC_CC
Cuts at: 3619 3619
Size: 7817
SapI GCTCTTCn'nnn_
Cuts at: 2340 5840 6050 2340
Size: 3500 210 4107
Fragments arranged by size:
4107 3500 210
SbfI CC_TGCA'GG
Cuts at: 4864 4864
Size: 7817
ScaI AGT'ACT
Cuts at: 3699 4051 7334 3699
Size: 352 3283 4182
Fragments arranged by size:
4182 3283 352
SfcI C'TryA_G
Cuts at: 33 315 1664 1779 2620 3103 4860 5545 6538
Size: 282 1349 115 841 483 1757 685 993
Cuts at: 6538 6729 33
Size: 191 1121
Fragments arranged by size:
1757 1349 1121 993 841 685 483 282
191 115
SfoI GGC'GCC
Cuts at: 131 389 504 772 5249 5497 131
Size: 258 115 268 4477 248 2451
Fragments arranged by size:
4477 2451 268 258 248 115
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7817
SmaI CCC'GGG
Cuts at: 411 2173 4839 411
Size: 1762 2666 3389
Fragments arranged by size:
3389 2666 1762
SmlI C'TyrA_G
Cuts at: 727 775 1306 3831 3880 4972 5887 6379 6641
Size: 48 531 2525 49 1092 915 492 262
Cuts at: 6641 727
Size: 1903
Fragments arranged by size:
2525 1903 1092 915 531 492 262 49
48
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7817
SphI G_CATG'C
Cuts at: 1910 4870 5370 1910
Size: 2960 500 4357
Fragments arranged by size:
4357 2960 500
SspI AAT'ATT
Cuts at: 2506 2721 2793 3765 4699 2506
Size: 215 72 972 934 5624
Fragments arranged by size:
5624 972 934 215 72
StyI C'CwwG_G
Cuts at: 107 2048 2754 4178 4523 4951 5305 107
Size: 1941 706 1424 345 428 354 2619
Fragments arranged by size:
2619 1941 1424 706 428 354 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2272 3223 5400 5533 5992 6201 2272
Size: 951 2177 133 459 209 3888
Fragments arranged by size:
3888 2177 951 459 209 133
TatI w'GTAC_w
Cuts at: 1569 2589 3650 3697 4049 7332 1569
Size: 1020 1061 47 352 3283 2054
Fragments arranged by size:
3283 2054 1061 1020 352 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1443 1475 2394 2426 7334 7366 1443
Size: 32 919 32 4908 32 1894
Fragments arranged by size:
4908 1894 919 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5614 5614
Size: 7817
XbaI T'CTAG_A
Cuts at: 4848 4848
Size: 7817
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 762 2320 2761 3444 5312 762
Size: 1558 441 683 1868 3267
Fragments arranged by size:
3267 1868 1558 683 441
XmaI C'CCGG_G
Cuts at: 409 2171 4837 409
Size: 1762 2666 3389
Fragments arranged by size:
3389 2666 1762
ZraI GAC'GTC
Cuts at: 1020 4320 1020
Size: 3300 4517
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AloI ApaI AscI AvrII BaeI
BbvCI BmgBI BmtI BsiWI BstZ17I Bsu36I ClaI CspCI
HpaI MfeI NdeI NheI NruI PacI PmlI PsiI
PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI