Restriction map for
pF1KE0052
(Circular) (Six-base) MAPSORT of: pF1KE0052.seq Check: 5290 from: 1 to: 6374
pF1KE0052 6374 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 2418 2843 3412 2418
Size: 425 569 5380
Fragments arranged by size:
5380 569 425
Acc65I G'GTAC_C
Cuts at: 3390 3390
Size: 6374
AclI AA'CG_TT
Cuts at: 2594 6214 2594
Size: 3620 2754
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 1030 1085 1132 1631 3286 4199 4631 5378
Size: 906 55 47 499 1655 913 432 747
Cuts at: 5378 5646 124
Size: 268 852
Fragments arranged by size:
1655 913 906 852 747 499 432 268
55 47
AflII C'TTAA_G
Cuts at: 2029 2029
Size: 6374
AflIII A'CryG_T
Cuts at: 325 3726 4830 5807 325
Size: 3401 1104 977 892
Fragments arranged by size:
3401 1104 977 892
AgeI A'CCGG_T
Cuts at: 4762 4762
Size: 6374
AleI CACnn'nnGTG
Cuts at: 861 2851 861
Size: 1990 4384
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 658 690 658
Size: 32 6342
AlwNI CAG_nnn'CTG
Cuts at: 3679 5246 3679
Size: 1567 4807
ApaLI G'TGCA_C
Cuts at: 5144 5144
Size: 6374
ApoI r'AATT_y
Cuts at: 535 763 1293 1846 1920 2131 2444 3260 3306
Size: 228 530 553 74 211 313 816 46
Cuts at: 3306 3378 5727 5983 535
Size: 72 2349 256 926
Fragments arranged by size:
2349 926 816 553 530 313 256 228
211 74 72 46
AseI AT'TA_AT
Cuts at: 20 991 1415 2363 2678 20
Size: 971 424 948 315 3716
Fragments arranged by size:
3716 971 948 424 315
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6374
AvaI C'yCGr_G
Cuts at: 280 3394 280
Size: 3114 3260
BamHI G'GATC_C
Cuts at: 3399 3399
Size: 6374
BanI G'GyrC_C
Cuts at: 284 3390 3804 4052 4087 284
Size: 3106 414 248 35 2571
Fragments arranged by size:
3106 2571 414 248 35
BanII G_rGCy'C
Cuts at: 248 3022 3388 4418 248
Size: 2774 366 1030 2204
Fragments arranged by size:
2774 2204 1030 366
BbeI G_GCGC'C
Cuts at: 3808 4056 3808
Size: 248 6126
BbsI GAAGACnn'nnnn_
Cuts at: 688 1152 1555 1756 2543 3284 688
Size: 464 403 201 787 741 3778
Fragments arranged by size:
3778 787 741 464 403 201
BciVI GTATCCnnnnn_n'
Cuts at: 4272 5033 6374 4272
Size: 761 1341 4272
Fragments arranged by size:
4272 1341 761
BclI T'GATC_A
Cuts at: 3894 3894
Size: 6374
BfrBI ATG'CAT
Cuts at: 988 1309 2464 2749 988
Size: 321 1155 285 4613
Fragments arranged by size:
4613 1155 321 285
BglII A'GATC_T
Cuts at: 2013 2665 3889 5926 2013
Size: 652 1224 2037 2461
Fragments arranged by size:
2461 2037 1224 652
BlpI GC'TnA_GC
Cuts at: 3486 3486
Size: 6374
Bme1580I G_kGCm'C
Cuts at: 1037 3999 4092 5148 1037
Size: 2962 93 1056 2263
Fragments arranged by size:
2962 2263 1056 93
BmgBI CAC'GTC
Cuts at: 204 204
Size: 6374
BmrI ACTGGGnnnn_n'
Cuts at: 1979 3586 3667 4002 6146 1979
Size: 1607 81 335 2144 2207
Fragments arranged by size:
2207 2144 1607 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 716 4741 716
Size: 4025 2349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 943 2975 3044 3126 501
Size: 442 2032 69 82 3749
Fragments arranged by size:
3749 2032 442 82 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2097 3550 4429 4921 5219 2097
Size: 1453 879 492 298 3252
Fragments arranged by size:
3252 1453 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 519 6078 519
Size: 5559 815
BsaAI yAC'GTr
Cuts at: 3727 3727
Size: 6374
BsaBI GATnn'nnATC
Cuts at: 6 1448 1475 3888 5925 6
Size: 1442 27 2413 2037 455
Fragments arranged by size:
2413 2037 1442 455 27
BsaHI Gr'CG_yC
Cuts at: 236 2118 3805 4053 4755 6251 236
Size: 1882 1687 248 702 1496 359
Fragments arranged by size:
1882 1687 1496 702 359 248
BsaWI w'CCGG_w
Cuts at: 2903 3563 4084 4762 5036 5183 5594 2903
Size: 660 521 678 274 147 411 3683
Fragments arranged by size:
3683 678 660 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 137 167 137
Size: 30 6344
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 223 256 2800 2905 2964 190
Size: 33 33 2544 105 59 3600
Fragments arranged by size:
3600 2544 105 59 33 33
BseYI C'CCAG_C
Cuts at: 3040 3799 4557 5134 5630 3040
Size: 759 758 577 496 3784
Fragments arranged by size:
3784 759 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3916 3962 5170 104
Size: 3812 46 1208 1308
Fragments arranged by size:
3812 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 248 492 1937 3022 3388 4166 4356 5148 248
Size: 244 1445 1085 366 778 190 792 1474
Fragments arranged by size:
1474 1445 1085 792 778 366 244 190
BsmI GAATG_Cn'
Cuts at: 990 1647 2466 2913 990
Size: 657 819 447 4451
Fragments arranged by size:
4451 819 657 447
Bsp1286I G_dGCh'C
Cuts at: 248 492 1037 1937 3022 3388 3999 4092 4166
Size: 244 545 900 1085 366 611 93 74
Cuts at: 4166 4356 4418 5148 248
Size: 190 62 730 1474
Fragments arranged by size:
1474 1085 900 730 611 545 366 244
190 93 74 62
BspEI T'CCGG_A
Cuts at: 2903 5594 2903
Size: 2691 3683
BspHI T'CATG_A
Cuts at: 5550 5550
Size: 6374
BspMI ACCTGCnnnn'nnnn_
Cuts at: 137 3424 3638 3940 4321 137
Size: 3287 214 302 381 2190
Fragments arranged by size:
3287 2190 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4666 4720 6204 6369 50
Size: 4616 54 1484 165 55
Fragments arranged by size:
4616 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2613 4286 2613
Size: 1673 4701
BsrFI r'CCGG_y
Cuts at: 4372 4553 4762 4372
Size: 181 209 5984
Fragments arranged by size:
5984 209 181
BssSI C'ACGA_G
Cuts at: 4645 5003 4645
Size: 358 6016
BstAPI GCAn_nnn'nTGC
Cuts at: 3751 3751
Size: 6374
BstBI TT'CG_AA
Cuts at: 4735 4735
Size: 6374
BstEII G'GTnAC_C
Cuts at: 1381 1381
Size: 6374
BstXI CCAn_nnnn'nTGG
Cuts at: 168 168
Size: 6374
BstYI r'GATC_y
Cuts at: 1 165 2013 2665 3399 3866 3889 4224 4470
Size: 164 1848 652 734 467 23 335 246
Cuts at: 4470 5482 5568 5580 5926 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1848 1012 734 652 467 449 346 335
246 164 86 23 12
BtgI C'CryG_G
Cuts at: 107 862 1840 3010 107
Size: 755 978 1170 3471
Fragments arranged by size:
3471 1170 978 755
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 927 1179 1316 4324 4505 4668 927
Size: 252 137 3008 181 163 2633
Fragments arranged by size:
3008 2633 252 181 163 137
BtsI GCAGTG_nn'
Cuts at: 258 3617 258
Size: 3359 3015
DraI TTT'AAA
Cuts at: 795 3373 795
Size: 2578 3796
DraIII CAC_nnn'GTG
Cuts at: 1781 1781
Size: 6374
DrdI GACnn_nn'nnGTC
Cuts at: 2031 4080 4938 2031
Size: 2049 858 3467
Fragments arranged by size:
3467 2049 858
EaeI y'GGCC_r
Cuts at: 110 3959 4133 4524 4551 5896 110
Size: 3849 174 391 27 1345 588
Fragments arranged by size:
3849 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 3959 3959
Size: 6374
EarI CTCTTCn'nnn_
Cuts at: 212 662 836 959 2213 3010 3123 4397 4607
Size: 450 174 123 1254 797 113 1274 210
Cuts at: 4607 212
Size: 1979
Fragments arranged by size:
1979 1274 1254 797 450 210 174 123
113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1705 4582 4892 5038 6184 1705
Size: 2877 310 146 1146 1895
Fragments arranged by size:
2877 1895 1146 310 146
EcoICRI GAG'CTC
Cuts at: 246 3020 3386 246
Size: 2774 366 3234
Fragments arranged by size:
3234 2774 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 501 943 1030 1085 1132 1631 2975 3044
Size: 377 442 87 55 47 499 1344 69
Cuts at: 3044 3126 3286 4199 4631 5378 5646 124
Size: 82 160 913 432 747 268 852
Fragments arranged by size:
1344 913 852 747 499 442 432 377
268 160 87 82 69 55 47
EcoO109I rG'GnC_Cy
Cuts at: 145 3037 3513 145
Size: 2892 476 3006
Fragments arranged by size:
3006 2892 476
EcoRI G'AATT_C
Cuts at: 763 1293 3378 763
Size: 530 2085 3759
Fragments arranged by size:
3759 2085 530
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2598 4393 2598
Size: 1795 4579
FspI TGC'GCA
Cuts at: 4155 4155
Size: 6374
HaeII r_GCGC'y
Cuts at: 3808 4056 5078 6057 3808
Size: 248 1022 979 4125
Fragments arranged by size:
4125 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 137 169 205 237 2989 3021 4216 4246 4248
Size: 32 36 32 2752 32 1195 30 2
Cuts at: 4248 4278 137
Size: 30 2233
Fragments arranged by size:
2752 2233 1195 36 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1681 3413 1681
Size: 1732 4642
HindIII A'AGCT_T
Cuts at: 1951 1951
Size: 6374
KasI G'GCGC_C
Cuts at: 3804 4052 3804
Size: 248 6126
KpnI G_GTAC'C
Cuts at: 3394 3394
Size: 6374
MluI A'CGCG_T
Cuts at: 5807 5807
Size: 6374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 907 1571 2188 5045 5229 907
Size: 664 617 2857 184 2052
Fragments arranged by size:
2857 2052 664 617 184
MscI TGG'CCA
Cuts at: 112 4135 112
Size: 4023 2351
MslI CAynn'nnrTG
Cuts at: 485 861 1040 1241 2851 4363 4490 485
Size: 376 179 201 1610 1512 127 2369
Fragments arranged by size:
2369 1610 1512 376 201 179 127
MspA1I CmG'CkG
Cuts at: 963 1440 1738 2867 3485 3799 4159 5172 5417
Size: 477 298 1129 618 314 360 1013 245
Cuts at: 5417 963
Size: 1920
Fragments arranged by size:
1920 1129 1013 618 477 360 314 298
245
NaeI GCC'GGC
Cuts at: 4555 4555
Size: 6374
NarI GG'CG_CC
Cuts at: 3805 4053 3805
Size: 248 6126
NcoI C'CATG_G
Cuts at: 107 1840 107
Size: 1733 4641
NdeI CA'TA_TG
Cuts at: 1041 1082 1143 1041
Size: 41 61 6272
Fragments arranged by size:
6272 61 41
NgoMIV G'CCGG_C
Cuts at: 4553 4553
Size: 6374
NruI TCG'CGA
Cuts at: 3210 3210
Size: 6374
NsiI A_TGCA'T
Cuts at: 990 1311 2466 2751 990
Size: 321 1155 285 4613
Fragments arranged by size:
4613 1155 321 285
NspI r_CATG'y
Cuts at: 329 2749 2842 3427 3927 4834 329
Size: 2420 93 585 500 907 1869
Fragments arranged by size:
2420 1869 907 585 500 93
PasI CC'CwG_GG
Cuts at: 161 161
Size: 6374
PciI A'CATG_T
Cuts at: 325 4830 325
Size: 4505 1869
PmeI GTTT'AAAC
Cuts at: 3373 3373
Size: 6374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 211 243 5513 5545 211
Size: 32 5270 32 1040
Fragments arranged by size:
5270 1040 32 32
PpuMI rG'GwC_Cy
Cuts at: 145 3037 145
Size: 2892 3482
PstI C_TGCA'G
Cuts at: 253 2277 3421 4106 253
Size: 2024 1144 685 2521
Fragments arranged by size:
2521 2024 1144 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6374
PvuII CAG'CTG
Cuts at: 963 1440 1738 3799 4159 963
Size: 477 298 2061 360 3178
Fragments arranged by size:
3178 2061 477 360 298
RsrII CG'GwC_CG
Cuts at: 4569 4569
Size: 6374
SacI G_AGCT'C
Cuts at: 248 3022 3388 248
Size: 2774 366 3234
Fragments arranged by size:
3234 2774 366
SalI G'TCGA_C
Cuts at: 3411 3411
Size: 6374
SapI GCTCTTCn'nnn_
Cuts at: 3010 4397 4607 3010
Size: 1387 210 4777
Fragments arranged by size:
4777 1387 210
SbfI CC_TGCA'GG
Cuts at: 3421 3421
Size: 6374
ScaI AGT'ACT
Cuts at: 5891 5891
Size: 6374
SexAI A'CCwGG_T
Cuts at: 1995 1995
Size: 6374
SfcI C'TryA_G
Cuts at: 33 249 891 1700 1724 1895 2273 3068 3417
Size: 216 642 809 24 171 378 795 349
Cuts at: 3417 4102 5095 5286 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 809 795 685 642 378 349
216 191 171 24
SfoI GGC'GCC
Cuts at: 3806 4054 3806
Size: 248 6126
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6374
SmaI CCC'GGG
Cuts at: 282 3396 282
Size: 3114 3260
SmlI C'TyrA_G
Cuts at: 2029 2112 3529 4444 4936 5198 2029
Size: 83 1417 915 492 262 3205
Fragments arranged by size:
3205 1417 915 492 262 83
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6374
SphI G_CATG'C
Cuts at: 3427 3927 3427
Size: 500 5874
SspI AAT'ATT
Cuts at: 2731 2731
Size: 6374
StuI AGG'CCT
Cuts at: 3115 3115
Size: 6374
StyI C'CwwG_G
Cuts at: 107 1231 1840 1962 3508 3862 107
Size: 1124 609 122 1546 354 2619
Fragments arranged by size:
2619 1546 1124 609 354 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 174 1902 3957 4090 4549 4758 174
Size: 1728 2055 133 459 209 1790
Fragments arranged by size:
2055 1790 1728 459 209 133
TatI w'GTAC_w
Cuts at: 5889 5889
Size: 6374
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1386 1418 5891 5923 1386
Size: 32 4473 32 1837
Fragments arranged by size:
4473 1837 32 32
Tth111I GACn'n_nGTC
Cuts at: 1071 4171 1071
Size: 3100 3274
XbaI T'CTAG_A
Cuts at: 2520 3405 2520
Size: 885 5489
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 168 622 2682 3869 168
Size: 454 2060 1187 2673
Fragments arranged by size:
2673 2060 1187 454
XmaI C'CCGG_G
Cuts at: 280 3394 280
Size: 3114 3260
XmnI GAAnn'nnTTC
Cuts at: 767 2953 767
Size: 2186 4188
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI
NsiI NspI PasI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmtI Bpu10I BsgI BsiWI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HpaI MfeI NheI NotI PacI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI