Restriction map for
pF1KE0051
(Circular) (Six-base) MAPSORT of: pF1KE0051.seq Check: 2136 from: 1 to: 6116
pF1KE0051 6116 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1066 1066
Size: 6116
AccI GT'mk_AC
Cuts at: 494 3154 494
Size: 2660 3456
Acc65I G'GTAC_C
Cuts at: 1072 3132 1072
Size: 2060 4056
AclI AA'CG_TT
Cuts at: 5956 5956
Size: 6116
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 2260 3941 4373 5120 5388 484
Size: 1776 1681 432 747 268 1212
Fragments arranged by size:
1776 1681 1212 747 432 268
AflIII A'CryG_T
Cuts at: 3468 4572 5549 3468
Size: 1104 977 4035
Fragments arranged by size:
4035 1104 977
AgeI A'CCGG_T
Cuts at: 784 4504 784
Size: 3720 2396
AhdI GACnn_n'nnGTC
Cuts at: 510 1581 510
Size: 1071 5045
AleI CACnn'nnGTG
Cuts at: 787 1450 1480 1708 2281 787
Size: 663 30 228 573 4622
Fragments arranged by size:
4622 663 573 228 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 532 532
Size: 6116
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2839 2871 2839
Size: 32 6084
AlwNI CAG_nnn'CTG
Cuts at: 864 2600 2871 3421 4988 864
Size: 1736 271 550 1567 1992
Fragments arranged by size:
1992 1736 1567 550 271
ApaI G_GGCC'C
Cuts at: 2991 2991
Size: 6116
ApaLI G'TGCA_C
Cuts at: 2733 4886 2733
Size: 2153 3963
ApoI r'AATT_y
Cuts at: 1552 3120 5469 5725 1552
Size: 1568 2349 256 1943
Fragments arranged by size:
2349 1943 1568 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6116
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6116
AvaI C'yCGr_G
Cuts at: 533 698 735 1571 1619 1706 1805 2788 3136
Size: 165 37 836 48 87 99 983 348
Cuts at: 3136 533
Size: 3513
Fragments arranged by size:
3513 983 836 348 165 99 87 48
37
BamHI G'GATC_C
Cuts at: 1961 3087 3141 1961
Size: 1126 54 4936
Fragments arranged by size:
4936 1126 54
BanI G'GyrC_C
Cuts at: 739 793 1072 1168 1654 1699 1710 3132 3546
Size: 54 279 96 486 45 11 1422 414
Cuts at: 3546 3794 3829 739
Size: 248 35 3026
Fragments arranged by size:
3026 1422 486 414 279 248 96 54
45 35 11
BanII G_rGCy'C
Cuts at: 230 686 967 1936 2150 2513 2578 2588 2795
Size: 456 281 969 214 363 65 10 207
Cuts at: 2795 2914 2991 3047 3130 4160 230
Size: 119 77 56 83 1030 2186
Fragments arranged by size:
2186 1030 969 456 363 281 214 207
119 83 77 65 56 10
BbeI G_GCGC'C
Cuts at: 3550 3798 3550
Size: 248 5868
BbsI GAAGACnn'nnnn_
Cuts at: 253 287 1030 2769 253
Size: 34 743 1739 3600
Fragments arranged by size:
3600 1739 743 34
BbvCI CC'TCA_GC
Cuts at: 236 2684 236
Size: 2448 3668
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 306 340 2108 2142 306
Size: 34 1768 34 4280
Fragments arranged by size:
4280 1768 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4014 4775 6116 4014
Size: 761 1341 4014
Fragments arranged by size:
4014 1341 761
BclI T'GATC_A
Cuts at: 1751 1970 1976 3636 1751
Size: 219 6 1660 4231
Fragments arranged by size:
4231 1660 219 6
BfrBI ATG'CAT
Cuts at: 317 317
Size: 6116
BglI GCCn_nnn'nGGC
Cuts at: 112 457 760 1314 112
Size: 345 303 554 4914
Fragments arranged by size:
4914 554 345 303
BglII A'GATC_T
Cuts at: 3023 3631 5668 3023
Size: 608 2037 3471
Fragments arranged by size:
3471 2037 608
BlpI GC'TnA_GC
Cuts at: 3228 3228
Size: 6116
Bme1580I G_kGCm'C
Cuts at: 742 796 1362 1659 1768 1999 2737 2991 3741
Size: 54 566 297 109 231 738 254 750
Cuts at: 3741 3834 4890 742
Size: 93 1056 1968
Fragments arranged by size:
1968 1056 750 738 566 297 254 231
109 93 54
BmgBI CAC'GTC
Cuts at: 2122 2122
Size: 6116
BmrI ACTGGGnnnn_n'
Cuts at: 1991 2997 3328 3409 3744 5888 1991
Size: 1006 331 81 335 2144 2219
Fragments arranged by size:
2219 2144 1006 335 331 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 903 4483 903
Size: 3580 2536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 1262 1582 1882 226
Size: 1036 320 300 4460
Fragments arranged by size:
4460 1036 320 300
Bpu10I CC'TnA_GC
Cuts at: 236 2684 236
Size: 2448 3668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3004 3043 3292 4171 4663 4961 3004
Size: 39 249 879 492 298 4159
Fragments arranged by size:
4159 879 492 298 249 39
BsaI GGTCTCn'nnnn_
Cuts at: 160 349 809 1120 5820 160
Size: 189 460 311 4700 456
Fragments arranged by size:
4700 460 456 311 189
BsaAI yAC'GTr
Cuts at: 3469 3469
Size: 6116
BsaBI GATnn'nnATC
Cuts at: 6 1207 1975 3630 5667 6
Size: 1201 768 1655 2037 455
Fragments arranged by size:
2037 1655 1201 768 455
BsaHI Gr'CG_yC
Cuts at: 1667 1955 2219 2228 3547 3795 4497 5993 1667
Size: 288 264 9 1319 248 702 1496 1790
Fragments arranged by size:
1790 1496 1319 702 288 264 248 9
BsaWI w'CCGG_w
Cuts at: 784 3305 3826 4504 4778 4925 5336 784
Size: 2521 521 678 274 147 411 1564
Fragments arranged by size:
2521 1564 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1260 1290 2311 2341 1260
Size: 30 1021 30 5035
Fragments arranged by size:
5035 1021 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 292 1365 2059 2365 292
Size: 1073 694 306 4043
Fragments arranged by size:
4043 1073 694 306
BseYI C'CCAG_C
Cuts at: 1605 2514 2907 3541 4299 4876 5372 1605
Size: 909 393 634 758 577 496 2349
Fragments arranged by size:
2349 909 758 634 577 496 393
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1122 2497 2663 2920 1122
Size: 1375 166 257 4318
Fragments arranged by size:
4318 1375 257 166
BsiEI CG_ry'CG
Cuts at: 104 1677 2058 3658 3704 4912 104
Size: 1573 381 1600 46 1208 1308
Fragments arranged by size:
1600 1573 1308 1208 381 46
BsiHKAI G_wGCw'C
Cuts at: 230 2737 3130 3908 4098 4890 230
Size: 2507 393 778 190 792 1456
Fragments arranged by size:
2507 1456 792 778 393 190
BsmI GAATG_Cn'
Cuts at: 1101 1101
Size: 6116
BsmBI CGTCTCn'nnnn_
Cuts at: 1864 1864
Size: 6116
Bsp1286I G_dGCh'C
Cuts at: 230 686 742 796 967 1362 1659 1768 1936
Size: 456 56 54 171 395 297 109 168
Cuts at: 1936 1999 2150 2513 2578 2588 2737 2795 2914
Size: 63 151 363 65 10 149 58 119
Cuts at: 2914 2991 3047 3130 3741 3834 3908 4098 4160
Size: 77 56 83 611 93 74 190 62
Cuts at: 4160 4890 230
Size: 730 1456
Fragments arranged by size:
1456 730 611 456 395 363 297 190
171 168 151 149 119 109 93 83
77 74 65 63 62 58 56 56
54 10
BspEI T'CCGG_A
Cuts at: 5336 5336
Size: 6116
BspHI T'CATG_A
Cuts at: 1973 5292 1973
Size: 3319 2797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1066 2096 2186 3166 3380 3682 4063 1066
Size: 1030 90 980 214 302 381 3119
Fragments arranged by size:
3119 1030 980 381 302 214 90
BsrBI CCG'CTC
Cuts at: 50 118 435 4408 4462 5946 6111 50
Size: 68 317 3973 54 1484 165 55
Fragments arranged by size:
3973 1484 317 165 68 55 54
BsrDI GCAATG_nn'
Cuts at: 401 746 1374 2003 2258 2844 4028 401
Size: 345 628 629 255 586 1184 2489
Fragments arranged by size:
2489 1184 629 628 586 345 255
BsrFI r'CCGG_y
Cuts at: 754 784 1261 1725 2015 4114 4295 4504 754
Size: 30 477 464 290 2099 181 209 2366
Fragments arranged by size:
2366 2099 477 464 290 209 181 30
BssSI C'ACGA_G
Cuts at: 4387 4745 4387
Size: 358 5758
BstAPI GCAn_nnn'nTGC
Cuts at: 3493 3493
Size: 6116
BstBI TT'CG_AA
Cuts at: 4477 4477
Size: 6116
BstEII G'GTnAC_C
Cuts at: 2499 2820 2499
Size: 321 5795
BstXI CCAn_nnnn'nTGG
Cuts at: 789 1062 1452 1860 2169 2526 2802 789
Size: 273 390 408 309 357 276 4103
Fragments arranged by size:
4103 408 390 357 309 276 273
BstYI r'GATC_y
Cuts at: 1 809 1961 2269 3023 3087 3141 3608 3631
Size: 808 1152 308 754 64 54 467 23
Cuts at: 3631 3966 4212 5224 5310 5322 5668 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1152 1012 808 754 467 449 346 335
308 246 86 64 54 23 12
Bsu36I CC'TnA_GG
Cuts at: 1661 2459 1661
Size: 798 5318
BtgI C'CryG_G
Cuts at: 107 131 170 957 975 1004 2264 2282 2651
Size: 24 39 787 18 29 1260 18 369
Cuts at: 2651 2801 107
Size: 150 3422
Fragments arranged by size:
3422 1260 787 369 150 39 29 24
18 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4066 4247 4410 4066
Size: 181 163 5772
Fragments arranged by size:
5772 181 163
BtsI GCAGTG_nn'
Cuts at: 362 2276 2737 3028 3359 362
Size: 1914 461 291 331 3119
Fragments arranged by size:
3119 1914 461 331 291
DraI TTT'AAA
Cuts at: 3115 3115
Size: 6116
DraIII CAC_nnn'GTG
Cuts at: 3005 3005
Size: 6116
DrdI GACnn_nn'nnGTC
Cuts at: 654 2773 3822 4680 654
Size: 2119 1049 858 2090
Fragments arranged by size:
2119 2090 1049 858
EaeI y'GGCC_r
Cuts at: 308 776 936 1315 1346 1770 1799 2051 2285
Size: 468 160 379 31 424 29 252 234
Cuts at: 2285 3701 3875 4266 4293 5638 308
Size: 1416 174 391 27 1345 786
Fragments arranged by size:
1416 1345 786 468 424 391 379 252
234 174 160 31 29 27
EagI C'GGCC_G
Cuts at: 3701 3701
Size: 6116
EarI CTCTTCn'nnn_
Cuts at: 347 2611 4139 4349 347
Size: 2264 1528 210 2114
Fragments arranged by size:
2264 2114 1528 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1522 4324 4634 4780 5926 1522
Size: 2802 310 146 1146 1712
Fragments arranged by size:
2802 1712 1146 310 146
EcoICRI GAG'CTC
Cuts at: 228 3128 228
Size: 2900 3216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 484 1262 1582 1882 2260 3941 4373 5120
Size: 258 778 320 300 378 1681 432 747
Cuts at: 5120 5388 226
Size: 268 954
Fragments arranged by size:
1681 954 778 747 432 378 320 300
268 258
EcoNI CCTnn'n_nnAGG
Cuts at: 1495 2242 1495
Size: 747 5369
EcoO109I rG'GnC_Cy
Cuts at: 459 2463 2784 2988 3255 459
Size: 2004 321 204 267 3320
Fragments arranged by size:
3320 2004 321 267 204
EcoRI G'AATT_C
Cuts at: 1552 3120 1552
Size: 1568 4548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 867 4135 867
Size: 3268 2848
FspI TGC'GCA
Cuts at: 3897 3897
Size: 6116
HaeII r_GCGC'y
Cuts at: 3550 3798 4820 5799 3550
Size: 248 1022 979 3867
Fragments arranged by size:
3867 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 496 516 528 548 646 678 1567 1599 2311
Size: 20 12 20 98 32 889 32 712
Cuts at: 2311 2343 2851 2883 3958 3988 3990 4020 496
Size: 32 508 32 1075 30 2 30 2592
Fragments arranged by size:
2592 1075 889 712 508 98 32 32
32 32 30 30 20 20 12 2
HincII GTy'rAC
Cuts at: 633 708 2703 2958 3155 633
Size: 75 1995 255 197 3594
Fragments arranged by size:
3594 1995 255 197 75
HindIII A'AGCT_T
Cuts at: 1110 1110
Size: 6116
KasI G'GCGC_C
Cuts at: 3546 3794 3546
Size: 248 5868
KpnI G_GTAC'C
Cuts at: 1076 3136 1076
Size: 2060 4056
MluI A'CGCG_T
Cuts at: 5549 5549
Size: 6116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1181 2432 4787 4971 1060
Size: 121 1251 2355 184 2205
Fragments arranged by size:
2355 2205 1251 184 121
MscI TGG'CCA
Cuts at: 778 938 1348 1801 2287 3877 778
Size: 160 410 453 486 1590 3017
Fragments arranged by size:
3017 1590 486 453 410 160
MslI CAynn'nnrTG
Cuts at: 787 910 1450 1480 1708 1747 2203 2263 2281
Size: 123 540 30 228 39 456 60 18
Cuts at: 2281 2800 2830 4105 4232 787
Size: 519 30 1275 127 2671
Fragments arranged by size:
2671 1275 540 519 456 228 127 123
60 39 30 30 18
MspA1I CmG'CkG
Cuts at: 977 1679 1944 2106 2443 2481 2662 2715 3227
Size: 702 265 162 337 38 181 53 512
Cuts at: 3227 3541 3901 4914 5159 977
Size: 314 360 1013 245 1934
Fragments arranged by size:
1934 1013 702 512 360 337 314 265
245 181 162 53 38
NaeI GCC'GGC
Cuts at: 756 2017 4297 756
Size: 1261 2280 2575
Fragments arranged by size:
2575 2280 1261
NarI GG'CG_CC
Cuts at: 3547 3795 3547
Size: 248 5868
NcoI C'CATG_G
Cuts at: 107 957 2264 2801 107
Size: 850 1307 537 3422
Fragments arranged by size:
3422 1307 850 537
NgoMIV G'CCGG_C
Cuts at: 754 2015 4295 754
Size: 1261 2280 2575
Fragments arranged by size:
2575 2280 1261
NsiI A_TGCA'T
Cuts at: 319 319
Size: 6116
NspI r_CATG'y
Cuts at: 317 729 1905 3169 3669 4576 317
Size: 412 1176 1264 500 907 1857
Fragments arranged by size:
1857 1264 1176 907 500 412
PasI CC'CwG_GG
Cuts at: 547 1068 1131 2448 2526 2888 547
Size: 521 63 1317 78 362 3775
Fragments arranged by size:
3775 1317 521 362 78 63
PciI A'CATG_T
Cuts at: 4572 4572
Size: 6116
PflMI CCAn_nnn'nTGG
Cuts at: 3040 3040
Size: 6116
PfoI T'CCnGG_A
Cuts at: 1838 1859 1838
Size: 21 6095
PmeI GTTT'AAAC
Cuts at: 3115 3115
Size: 6116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5255 5287 5255
Size: 32 6084
PspOMI G'GGCC_C
Cuts at: 2987 2987
Size: 6116
PstI C_TGCA'G
Cuts at: 591 1047 2183 3163 3848 591
Size: 456 1136 980 685 2859
Fragments arranged by size:
2859 1136 980 685 456
PvuI CG_AT'CG
Cuts at: 104 2058 104
Size: 1954 4162
PvuII CAG'CTG
Cuts at: 1944 2481 2662 2715 3541 3901 1944
Size: 537 181 53 826 360 4159
Fragments arranged by size:
4159 826 537 360 181 53
RsrII CG'GwC_CG
Cuts at: 4311 4311
Size: 6116
SacI G_AGCT'C
Cuts at: 230 3130 230
Size: 2900 3216
SacII CC_GC'GG
Cuts at: 978 978
Size: 6116
SalI G'TCGA_C
Cuts at: 3153 3153
Size: 6116
SapI GCTCTTCn'nnn_
Cuts at: 2611 4139 4349 2611
Size: 1528 210 4378
Fragments arranged by size:
4378 1528 210
SbfI CC_TGCA'GG
Cuts at: 3163 3163
Size: 6116
ScaI AGT'ACT
Cuts at: 5633 5633
Size: 6116
SfcI C'TryA_G
Cuts at: 33 587 1043 2179 2706 3159 3844 4837 5028
Size: 554 456 1136 527 453 685 993 191
Cuts at: 5028 33
Size: 1121
Fragments arranged by size:
1136 1121 993 685 554 527 456 453
191
SfoI GGC'GCC
Cuts at: 3548 3796 3548
Size: 248 5868
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6116
SgrAI Cr'CCGG_yG
Cuts at: 784 784
Size: 6116
SmaI CCC'GGG
Cuts at: 1621 1708 1807 3138 1621
Size: 87 99 1331 4599
Fragments arranged by size:
4599 1331 99 87
SmlI C'TyrA_G
Cuts at: 3019 3058 3271 4186 4678 4940 3019
Size: 39 213 915 492 262 4195
Fragments arranged by size:
4195 915 492 262 213 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6116
SphI G_CATG'C
Cuts at: 317 1905 3169 3669 317
Size: 1588 1264 500 2764
Fragments arranged by size:
2764 1588 1264 500
StuI AGG'CCT
Cuts at: 763 763
Size: 6116
StyI C'CwwG_G
Cuts at: 107 764 890 957 1010 1379 2264 2579 2801
Size: 657 126 67 53 369 885 315 222
Cuts at: 2801 3250 3604 107
Size: 449 354 2619
Fragments arranged by size:
2619 885 657 449 369 354 315 222
126 67 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1879 2478 2518 2629 2764 2880 3016 3699 3832
Size: 599 40 111 135 116 136 683 133
Cuts at: 3832 4291 4500 1879
Size: 459 209 3495
Fragments arranged by size:
3495 683 599 459 209 136 135 133
116 111 40
TatI w'GTAC_w
Cuts at: 5631 5631
Size: 6116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1260 1292 5633 5665 1260
Size: 32 4341 32 1711
Fragments arranged by size:
4341 1711 32 32
Tth111I GACn'n_nGTC
Cuts at: 541 580 952 3913 541
Size: 39 372 2961 2744
Fragments arranged by size:
2961 2744 372 39
XbaI T'CTAG_A
Cuts at: 3147 3147
Size: 6116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 414 786 3611 414
Size: 372 2825 2919
Fragments arranged by size:
2919 2825 372
XmaI C'CCGG_G
Cuts at: 1619 1706 1805 3136 1619
Size: 87 99 1331 4599
Fragments arranged by size:
4599 1331 99 87
XmnI GAAnn'nnTTC
Cuts at: 1037 1249 1468 1552 2383 1037
Size: 212 219 84 831 4770
Fragments arranged by size:
4770 831 219 212 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI AvrII BaeI BmtI BsiWI
BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PmlI
PpuMI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SnaBI SrfI SspI SwaI XhoI ZraI