Restriction map for
pF1KE0049
(Circular) (Six-base) MAPSORT of: pF1KE0049.seq Check: 197 from: 1 to: 5294
pF1KE0049 5294 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 2332 2332
Size: 5294
Acc65I G'GTAC_C
Cuts at: 2310 2310
Size: 5294
AclI AA'CG_TT
Cuts at: 5134 5134
Size: 5294
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 3119 3551 4298 4566 145
Size: 2974 432 747 268 873
Fragments arranged by size:
2974 873 747 432 268
AflIII A'CryG_T
Cuts at: 2103 2646 3750 4727 2103
Size: 543 1104 977 2670
Fragments arranged by size:
2670 1104 977 543
AgeI A'CCGG_T
Cuts at: 3682 3682
Size: 5294
AleI CACnn'nnGTG
Cuts at: 758 1734 1736 758
Size: 976 2 4316
Fragments arranged by size:
4316 976 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 776 776
Size: 5294
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1043 1075 1043
Size: 32 5262
AlwNI CAG_nnn'CTG
Cuts at: 1072 2599 4166 1072
Size: 1527 1567 2200
Fragments arranged by size:
2200 1567 1527
ApaLI G'TGCA_C
Cuts at: 4064 4064
Size: 5294
ApoI r'AATT_y
Cuts at: 703 1397 2080 2212 2255 2298 4647 4903 703
Size: 694 683 132 43 43 2349 256 1094
Fragments arranged by size:
2349 1094 694 683 256 132 43 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 5294
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5294
AvaI C'yCGr_G
Cuts at: 2314 2314
Size: 5294
BamHI G'GATC_C
Cuts at: 2277 2319 2277
Size: 42 5252
BanI G'GyrC_C
Cuts at: 760 2310 2724 2972 3007 760
Size: 1550 414 248 35 3047
Fragments arranged by size:
3047 1550 414 248 35
BanII G_rGCy'C
Cuts at: 2122 2308 3338 2122
Size: 186 1030 4078
Fragments arranged by size:
4078 1030 186
BbeI G_GCGC'C
Cuts at: 2728 2976 2728
Size: 248 5046
BbsI GAAGACnn'nnnn_
Cuts at: 2207 2207
Size: 5294
BbvCI CC'TCA_GC
Cuts at: 986 986
Size: 5294
BciVI GTATCCnnnnn_n'
Cuts at: 3192 3953 5294 3192
Size: 761 1341 3192
Fragments arranged by size:
3192 1341 761
BclI T'GATC_A
Cuts at: 675 2814 675
Size: 2139 3155
BglII A'GATC_T
Cuts at: 2809 4846 2809
Size: 2037 3257
BlpI GC'TnA_GC
Cuts at: 2406 2406
Size: 5294
Bme1580I G_kGCm'C
Cuts at: 1428 2919 3012 4068 1428
Size: 1491 93 1056 2654
Fragments arranged by size:
2654 1491 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 293 643 1966 2506 2587 2922 5066 293
Size: 350 1323 540 81 335 2144 521
Fragments arranged by size:
2144 1323 540 521 350 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3661 3661
Size: 5294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 797 1389 1464 758
Size: 39 592 75 4588
Fragments arranged by size:
4588 592 75 39
Bpu10I CC'TnA_GC
Cuts at: 854 986 854
Size: 132 5162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 701 2470 3349 3841 4139 701
Size: 1769 879 492 298 1856
Fragments arranged by size:
1856 1769 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1367 2191 4998 1367
Size: 824 2807 1663
Fragments arranged by size:
2807 1663 824
BsaAI yAC'GTr
Cuts at: 1429 2647 1429
Size: 1218 4076
BsaBI GATnn'nnATC
Cuts at: 6 2808 4845 6
Size: 2802 2037 455
Fragments arranged by size:
2802 2037 455
BsaHI Gr'CG_yC
Cuts at: 2725 2973 3675 5171 2725
Size: 248 702 1496 2848
Fragments arranged by size:
2848 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2483 3004 3682 3956 4103 4514 2483
Size: 521 678 274 147 411 3263
Fragments arranged by size:
3263 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1043 1073 1043
Size: 30 5264
BseYI C'CCAG_C
Cuts at: 1342 2719 3477 4054 4550 1342
Size: 1377 758 577 496 2086
Fragments arranged by size:
2086 1377 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 154
Size: 5294
BsiEI CG_ry'CG
Cuts at: 104 2836 2882 4090 104
Size: 2732 46 1208 1308
Fragments arranged by size:
2732 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 281 855 1970 2308 3086 3276 4068 281
Size: 574 1115 338 778 190 792 1507
Fragments arranged by size:
1507 1115 792 778 574 338 190
BsmI GAATG_Cn'
Cuts at: 1842 1842
Size: 5294
Bsp1286I G_dGCh'C
Cuts at: 281 855 1428 1970 2122 2308 2919 3012 3086
Size: 574 573 542 152 186 611 93 74
Cuts at: 3086 3276 3338 4068 281
Size: 190 62 730 1507
Fragments arranged by size:
1507 730 611 574 573 542 190 186
152 93 74 62
BspEI T'CCGG_A
Cuts at: 4514 4514
Size: 5294
BspHI T'CATG_A
Cuts at: 4470 4470
Size: 5294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2344 2558 2860 3241 2344
Size: 214 302 381 4397
Fragments arranged by size:
4397 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3586 3640 5124 5289 50
Size: 3536 54 1484 165 55
Fragments arranged by size:
3536 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 428 842 1851 3206 428
Size: 414 1009 1355 2516
Fragments arranged by size:
2516 1355 1009 414
BsrFI r'CCGG_y
Cuts at: 3292 3473 3682 3292
Size: 181 209 4904
Fragments arranged by size:
4904 209 181
BssSI C'ACGA_G
Cuts at: 1732 3565 3923 1732
Size: 1833 358 3103
Fragments arranged by size:
3103 1833 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 2671 1123
Size: 1548 3746
BstBI TT'CG_AA
Cuts at: 708 3655 708
Size: 2947 2347
BstXI CCAn_nnnn'nTGG
Cuts at: 423 423
Size: 5294
BstYI r'GATC_y
Cuts at: 1 621 1155 1482 2277 2319 2786 2809 3144
Size: 620 534 327 795 42 467 23 335
Cuts at: 3144 3390 4402 4488 4500 4846 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 795 620 534 467 449 346 335
327 246 86 42 23 12
BtgI C'CryG_G
Cuts at: 337 337
Size: 5294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3244 3425 3588 3244
Size: 181 163 4950
Fragments arranged by size:
4950 181 163
BtsI GCAGTG_nn'
Cuts at: 734 1073 2537 734
Size: 339 1464 3491
Fragments arranged by size:
3491 1464 339
DraI TTT'AAA
Cuts at: 2085 2293 2085
Size: 208 5086
DrdI GACnn_nn'nnGTC
Cuts at: 3000 3858 3000
Size: 858 4436
EaeI y'GGCC_r
Cuts at: 265 413 1551 2879 3053 3444 3471 4816 265
Size: 148 1138 1328 174 391 27 1345 743
Fragments arranged by size:
1345 1328 1138 743 391 174 148 27
EagI C'GGCC_G
Cuts at: 2879 2879
Size: 5294
EarI CTCTTCn'nnn_
Cuts at: 183 1203 1564 1958 3317 3527 183
Size: 1020 361 394 1359 210 1950
Fragments arranged by size:
1950 1359 1020 394 361 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3502 3812 3958 5104 3502
Size: 310 146 1146 3692
Fragments arranged by size:
3692 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2306 2306
Size: 5294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 758 797 1389 1464 3119 3551 4298 4566
Size: 613 39 592 75 1655 432 747 268
Cuts at: 4566 145
Size: 873
Fragments arranged by size:
1655 873 747 613 592 432 268 75
39
EcoNI CCTnn'n_nnAGG
Cuts at: 1385 1385
Size: 5294
EcoO109I rG'GnC_Cy
Cuts at: 611 644 2433 611
Size: 33 1789 3472
Fragments arranged by size:
3472 1789 33
EcoRI G'AATT_C
Cuts at: 2298 2298
Size: 5294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1262 1417 3313 1262
Size: 155 1896 3243
Fragments arranged by size:
3243 1896 155
FspI TGC'GCA
Cuts at: 3075 3075
Size: 5294
HaeII r_GCGC'y
Cuts at: 2728 2976 3998 4977 2728
Size: 248 1022 979 3045
Fragments arranged by size:
3045 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3136 3166 3168 3198 3136
Size: 30 2 30 5232
Fragments arranged by size:
5232 30 30 2
HincII GTy'rAC
Cuts at: 1440 1791 2333 1440
Size: 351 542 4401
Fragments arranged by size:
4401 542 351
HindIII A'AGCT_T
Cuts at: 452 452
Size: 5294
HpaI GTT'AAC
Cuts at: 1791 1791
Size: 5294
KasI G'GCGC_C
Cuts at: 2724 2972 2724
Size: 248 5046
KpnI G_GTAC'C
Cuts at: 2314 2314
Size: 5294
MfeI C'AATT_G
Cuts at: 1325 2159 1325
Size: 834 4460
MluI A'CGCG_T
Cuts at: 4727 4727
Size: 5294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 110 1878 3965 4149 110
Size: 1768 2087 184 1255
Fragments arranged by size:
2087 1768 1255 184
MscI TGG'CCA
Cuts at: 267 415 1553 3055 267
Size: 148 1138 1502 2506
Fragments arranged by size:
2506 1502 1138 148
MslI CAynn'nnrTG
Cuts at: 506 758 1351 1734 1736 2147 3283 3410 506
Size: 252 593 383 2 411 1136 127 2390
Fragments arranged by size:
2390 1136 593 411 383 252 127 2
MspA1I CmG'CkG
Cuts at: 1864 2405 2719 3079 4092 4337 1864
Size: 541 314 360 1013 245 2821
Fragments arranged by size:
2821 1013 541 360 314 245
NaeI GCC'GGC
Cuts at: 3475 3475
Size: 5294
NarI GG'CG_CC
Cuts at: 2725 2973 2725
Size: 248 5046
NcoI C'CATG_G
Cuts at: 337 337
Size: 5294
NgoMIV G'CCGG_C
Cuts at: 3473 3473
Size: 5294
NspI r_CATG'y
Cuts at: 823 934 1350 2107 2347 2847 3754 823
Size: 111 416 757 240 500 907 2363
Fragments arranged by size:
2363 907 757 500 416 240 111
PciI A'CATG_T
Cuts at: 2103 3750 2103
Size: 1647 3647
PfoI T'CCnGG_A
Cuts at: 813 2280 813
Size: 1467 3827
PmeI GTTT'AAAC
Cuts at: 2293 2293
Size: 5294
PmlI CAC'GTG
Cuts at: 1429 1429
Size: 5294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1043 1075 4433 4465 1043
Size: 32 3358 32 1872
Fragments arranged by size:
3358 1872 32 32
PpuMI rG'GwC_Cy
Cuts at: 644 644
Size: 5294
PstI C_TGCA'G
Cuts at: 891 1389 2047 2341 3026 891
Size: 498 658 294 685 3159
Fragments arranged by size:
3159 685 658 498 294
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5294
PvuII CAG'CTG
Cuts at: 1864 2719 3079 1864
Size: 855 360 4079
Fragments arranged by size:
4079 855 360
RsrII CG'GwC_CG
Cuts at: 3489 3489
Size: 5294
SacI G_AGCT'C
Cuts at: 2308 2308
Size: 5294
SalI G'TCGA_C
Cuts at: 2331 2331
Size: 5294
SapI GCTCTTCn'nnn_
Cuts at: 183 1958 3317 3527 183
Size: 1775 1359 210 1950
Fragments arranged by size:
1950 1775 1359 210
SbfI CC_TGCA'GG
Cuts at: 2341 2341
Size: 5294
ScaI AGT'ACT
Cuts at: 4811 4811
Size: 5294
SfcI C'TryA_G
Cuts at: 33 887 914 1223 1385 2043 2337 3022 4015
Size: 854 27 309 162 658 294 685 993
Cuts at: 4015 4206 33
Size: 191 1121
Fragments arranged by size:
1121 993 854 685 658 309 294 191
162 27
SfoI GGC'GCC
Cuts at: 2726 2974 2726
Size: 248 5046
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5294
SmaI CCC'GGG
Cuts at: 2316 2316
Size: 5294
SmlI C'TyrA_G
Cuts at: 716 2449 3364 3856 4118 716
Size: 1733 915 492 262 1892
Fragments arranged by size:
1892 1733 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5294
SphI G_CATG'C
Cuts at: 934 1350 2347 2847 934
Size: 416 997 500 3381
Fragments arranged by size:
3381 997 500 416
StuI AGG'CCT
Cuts at: 1336 1336
Size: 5294
StyI C'CwwG_G
Cuts at: 337 640 1420 2428 2782 337
Size: 303 780 1008 354 2849
Fragments arranged by size:
2849 1008 780 354 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1756 1985 2877 3010 3469 3678 1756
Size: 229 892 133 459 209 3372
Fragments arranged by size:
3372 892 459 229 209 133
TatI w'GTAC_w
Cuts at: 1650 1752 4809 1650
Size: 102 3057 2135
Fragments arranged by size:
3057 2135 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4811 4843 4811
Size: 32 5262
Tth111I GACn'n_nGTC
Cuts at: 580 868 3091 580
Size: 288 2223 2783
Fragments arranged by size:
2783 2223 288
XbaI T'CTAG_A
Cuts at: 2325 2325
Size: 5294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1167 1350 2789 1167
Size: 183 1439 3672
Fragments arranged by size:
3672 1439 183
XmaI C'CCGG_G
Cuts at: 2314 2314
Size: 5294
XmnI GAAnn'nnTTC
Cuts at: 923 1397 923
Size: 474 4820
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BseRI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI