Restriction map for
pF1KE0046
(Circular) (Six-base) MAPSORT of: pF1KE0046.seq Check: 5282 from: 1 to: 5030
pF1KE0046 5030 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1643 1643
Size: 5030
AccI GT'mk_AC
Cuts at: 2068 2068
Size: 5030
Acc65I G'GTAC_C
Cuts at: 842 2046 842
Size: 1204 3826
AclI AA'CG_TT
Cuts at: 4870 4870
Size: 5030
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 1293 1581 2855 3287 4034 4302 154
Size: 1139 288 1274 432 747 268 882
Fragments arranged by size:
1274 1139 882 747 432 288 268
AfeI AGC'GCT
Cuts at: 1483 1483
Size: 5030
AflIII A'CryG_T
Cuts at: 1862 2382 3486 4463 1862
Size: 520 1104 977 2429
Fragments arranged by size:
2429 1104 977 520
AgeI A'CCGG_T
Cuts at: 3418 3418
Size: 5030
AhdI GACnn_n'nnGTC
Cuts at: 1227 1227
Size: 5030
AleI CACnn'nnGTG
Cuts at: 1369 1369
Size: 5030
AlwNI CAG_nnn'CTG
Cuts at: 755 2335 3902 755
Size: 1580 1567 1883
Fragments arranged by size:
1883 1580 1567
ApaI G_GGCC'C
Cuts at: 339 1271 339
Size: 932 4098
ApaLI G'TGCA_C
Cuts at: 3800 3800
Size: 5030
ApoI r'AATT_y
Cuts at: 2034 4383 4639 2034
Size: 2349 256 2425
Fragments arranged by size:
2425 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5030
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
AvaI C'yCGr_G
Cuts at: 332 1723 2050 332
Size: 1391 327 3312
Fragments arranged by size:
3312 1391 327
BamHI G'GATC_C
Cuts at: 1004 2055 1004
Size: 1051 3979
BanI G'GyrC_C
Cuts at: 731 842 954 1371 1663 1729 1822 2046 2460
Size: 111 112 417 292 66 93 224 414
Cuts at: 2460 2708 2743 731
Size: 248 35 3018
Fragments arranged by size:
3018 417 414 292 248 224 112 111
93 66 35
BanII G_rGCy'C
Cuts at: 339 433 909 1271 1568 1979 2044 3074 339
Size: 94 476 362 297 411 65 1030 2295
Fragments arranged by size:
2295 1030 476 411 362 297 94 65
BbeI G_GCGC'C
Cuts at: 1733 2464 2712 1733
Size: 731 248 4051
Fragments arranged by size:
4051 731 248
BbsI GAAGACnn'nnnn_
Cuts at: 690 690
Size: 5030
BbvCI CC'TCA_GC
Cuts at: 124 1272 124
Size: 1148 3882
BciVI GTATCCnnnnn_n'
Cuts at: 2928 3689 5030 2928
Size: 761 1341 2928
Fragments arranged by size:
2928 1341 761
BclI T'GATC_A
Cuts at: 2550 2550
Size: 5030
BglI GCCn_nnn'nGGC
Cuts at: 1742 1742
Size: 5030
BglII A'GATC_T
Cuts at: 492 2545 4582 492
Size: 2053 2037 940
Fragments arranged by size:
2053 2037 940
BlpI GC'TnA_GC
Cuts at: 2142 2142
Size: 5030
Bme1580I G_kGCm'C
Cuts at: 185 339 1271 2655 2748 3804 185
Size: 154 932 1384 93 1056 1411
Fragments arranged by size:
1411 1384 1056 932 154 93
BmrI ACTGGGnnnn_n'
Cuts at: 632 2242 2323 2658 4802 632
Size: 1610 81 335 2144 860
Fragments arranged by size:
2144 1610 860 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1044 3397 1044
Size: 2353 2677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 992 1771 1918 586
Size: 406 779 147 3698
Fragments arranged by size:
3698 779 406 147
Bpu10I CC'TnA_GC
Cuts at: 124 1272 124
Size: 1148 3882
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 788 2206 3085 3577 3875 788
Size: 1418 879 492 298 1943
Fragments arranged by size:
1943 1418 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4734 4734
Size: 5030
BsaAI yAC'GTr
Cuts at: 2383 2383
Size: 5030
BsaBI GATnn'nnATC
Cuts at: 6 491 2544 4581 6
Size: 485 2053 2037 455
Fragments arranged by size:
2053 2037 485 455
BsaHI Gr'CG_yC
Cuts at: 695 1730 1756 2461 2709 3411 4907 695
Size: 1035 26 705 248 702 1496 818
Fragments arranged by size:
1496 1035 818 705 702 248 26
BsaWI w'CCGG_w
Cuts at: 859 1001 2219 2740 3418 3692 3839 4250 859
Size: 142 1218 521 678 274 147 411 1639
Fragments arranged by size:
1639 1218 678 521 411 274 147 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1330 1360 1849 1879 1330
Size: 30 489 30 4481
Fragments arranged by size:
4481 489 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 917 1153 1546 917
Size: 236 393 4401
Fragments arranged by size:
4401 393 236
BseYI C'CCAG_C
Cuts at: 1589 2455 3213 3790 4286 1589
Size: 866 758 577 496 2333
Fragments arranged by size:
2333 866 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 289
Size: 5030
BsiEI CG_ry'CG
Cuts at: 104 1379 1929 2572 2618 3826 104
Size: 1275 550 643 46 1208 1308
Fragments arranged by size:
1308 1275 1208 643 550 46
BsiHKAI G_wGCw'C
Cuts at: 689 2044 2822 3012 3804 689
Size: 1355 778 190 792 1915
Fragments arranged by size:
1915 1355 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1747 1747
Size: 5030
Bsp1286I G_dGCh'C
Cuts at: 185 339 433 689 909 1271 1568 1979 2044
Size: 154 94 256 220 362 297 411 65
Cuts at: 2044 2655 2748 2822 3012 3074 3804 185
Size: 611 93 74 190 62 730 1411
Fragments arranged by size:
1411 730 611 411 362 297 256 220
190 154 94 93 74 65 62
BspEI T'CCGG_A
Cuts at: 4250 4250
Size: 5030
BspHI T'CATG_A
Cuts at: 483 4206 483
Size: 3723 1307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1643 1943 2080 2294 2596 2977 1643
Size: 300 137 214 302 381 3696
Fragments arranged by size:
3696 381 302 300 214 137
BsrBI CCG'CTC
Cuts at: 50 3322 3376 4860 5025 50
Size: 3272 54 1484 165 55
Fragments arranged by size:
3272 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2942 2942
Size: 5030
BsrFI r'CCGG_y
Cuts at: 1374 1834 3028 3209 3418 1374
Size: 460 1194 181 209 2986
Fragments arranged by size:
2986 1194 460 209 181
BssHII G'CGCG_C
Cuts at: 1606 1700 1772 1606
Size: 94 72 4864
Fragments arranged by size:
4864 94 72
BssSI C'ACGA_G
Cuts at: 1453 3301 3659 1453
Size: 1848 358 2824
Fragments arranged by size:
2824 1848 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2407 2407
Size: 5030
BstBI TT'CG_AA
Cuts at: 3391 3391
Size: 5030
BstEII G'GTnAC_C
Cuts at: 997 997
Size: 5030
BstYI r'GATC_y
Cuts at: 1 492 722 1004 2055 2522 2545 2880 3126
Size: 491 230 282 1051 467 23 335 246
Cuts at: 3126 4138 4224 4236 4582 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1051 1012 491 467 449 346 335 282
246 230 86 23 12
BtgI C'CryG_G
Cuts at: 107 155 1263 1277 1364 1385 1584 1639 1737
Size: 48 1108 14 87 21 199 55 98
Cuts at: 1737 1908 1923 107
Size: 171 15 3214
Fragments arranged by size:
3214 1108 199 171 98 87 55 48
21 15 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2980 3161 3324 2980
Size: 181 163 4686
Fragments arranged by size:
4686 181 163
BtsI GCAGTG_nn'
Cuts at: 277 1257 2273 277
Size: 980 1016 3034
Fragments arranged by size:
3034 1016 980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 932 967 932
Size: 35 4995
DraI TTT'AAA
Cuts at: 2029 2029
Size: 5030
DraIII CAC_nnn'GTG
Cuts at: 961 961
Size: 5030
DrdI GACnn_nn'nnGTC
Cuts at: 2736 3594 2736
Size: 858 4172
EaeI y'GGCC_r
Cuts at: 200 1376 1419 1926 2615 2789 3180 3207 4552
Size: 1176 43 507 689 174 391 27 1345
Cuts at: 4552 200
Size: 678
Fragments arranged by size:
1345 1176 689 678 507 391 174 43
27
EagI C'GGCC_G
Cuts at: 1376 1926 2615 1376
Size: 550 689 3791
Fragments arranged by size:
3791 689 550
EarI CTCTTCn'nnn_
Cuts at: 145 214 709 1957 3053 3263 145
Size: 69 495 1248 1096 210 1912
Fragments arranged by size:
1912 1248 1096 495 210 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1144 3238 3548 3694 4840 1144
Size: 2094 310 146 1146 1334
Fragments arranged by size:
2094 1334 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2042 2042
Size: 5030
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 586 992 1293 1581 1771 1918 2855 3287
Size: 432 406 301 288 190 147 937 432
Cuts at: 3287 4034 4302 154
Size: 747 268 882
Fragments arranged by size:
937 882 747 432 432 406 301 288
268 190 147
EcoNI CCTnn'n_nnAGG
Cuts at: 899 1135 899
Size: 236 4794
EcoO109I rG'GnC_Cy
Cuts at: 369 1038 1268 2169 369
Size: 669 230 901 3230
Fragments arranged by size:
3230 901 669 230
EcoRI G'AATT_C
Cuts at: 2034 2034
Size: 5030
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3049 3049
Size: 5030
FspI TGC'GCA
Cuts at: 2811 2811
Size: 5030
HaeII r_GCGC'y
Cuts at: 1485 1713 1733 1796 2464 2712 3734 4713 1485
Size: 228 20 63 668 248 1022 979 1802
Fragments arranged by size:
1802 1022 979 668 248 228 63 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1213 1245 1330 1362 1744 1776 1849 1881 2872
Size: 32 85 32 382 32 73 32 991
Cuts at: 2872 2902 2904 2934 1213
Size: 30 2 30 3309
Fragments arranged by size:
3309 991 382 85 73 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2069 2069
Size: 5030
HindIII A'AGCT_T
Cuts at: 508 508
Size: 5030
KasI G'GCGC_C
Cuts at: 1729 2460 2708 1729
Size: 731 248 4051
Fragments arranged by size:
4051 731 248
KpnI G_GTAC'C
Cuts at: 846 2050 846
Size: 1204 3826
MluI A'CGCG_T
Cuts at: 1862 4463 1862
Size: 2601 2429
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1316 1892 2026 3701 3885 1316
Size: 576 134 1675 184 2461
Fragments arranged by size:
2461 1675 576 184 134
MscI TGG'CCA
Cuts at: 202 2791 202
Size: 2589 2441
MslI CAynn'nnrTG
Cuts at: 925 1369 3019 3146 925
Size: 444 1650 127 2809
Fragments arranged by size:
2809 1650 444 127
MspA1I CmG'CkG
Cuts at: 527 752 770 1265 1425 1505 1586 1641 1739
Size: 225 18 495 160 80 81 55 98
Cuts at: 1739 1910 1925 2141 2455 2815 3828 4073 527
Size: 171 15 216 314 360 1013 245 1484
Fragments arranged by size:
1484 1013 495 360 314 245 225 216
171 160 98 81 80 55 18 15
NaeI GCC'GGC
Cuts at: 1376 1836 3211 1376
Size: 460 1375 3195
Fragments arranged by size:
3195 1375 460
NarI GG'CG_CC
Cuts at: 1730 2461 2709 1730
Size: 731 248 4051
Fragments arranged by size:
4051 731 248
NcoI C'CATG_G
Cuts at: 107 155 1277 1385 107
Size: 48 1122 108 3752
Fragments arranged by size:
3752 1122 108 48
NgoMIV G'CCGG_C
Cuts at: 1374 1834 3209 1374
Size: 460 1375 3195
Fragments arranged by size:
3195 1375 460
NotI GC'GGCC_GC
Cuts at: 1926 1926
Size: 5030
NspI r_CATG'y
Cuts at: 324 924 2083 2583 3490 324
Size: 600 1159 500 907 1864
Fragments arranged by size:
1864 1159 907 600 500
PasI CC'CwG_GG
Cuts at: 444 444
Size: 5030
PciI A'CATG_T
Cuts at: 3486 3486
Size: 5030
PmeI GTTT'AAAC
Cuts at: 2029 2029
Size: 5030
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 4169
Size: 32 4998
PpuMI rG'GwC_Cy
Cuts at: 369 1038 369
Size: 669 4361
PspOMI G'GGCC_C
Cuts at: 335 1267 335
Size: 932 4098
PstI C_TGCA'G
Cuts at: 264 2077 2762 264
Size: 1813 685 2532
Fragments arranged by size:
2532 1813 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5030
PvuII CAG'CTG
Cuts at: 752 2455 2815 752
Size: 1703 360 2967
Fragments arranged by size:
2967 1703 360
RsrII CG'GwC_CG
Cuts at: 3225 3225
Size: 5030
SacI G_AGCT'C
Cuts at: 2044 2044
Size: 5030
SacII CC_GC'GG
Cuts at: 1266 1587 1642 1740 1911 1926 1266
Size: 321 55 98 171 15 4370
Fragments arranged by size:
4370 321 171 98 55 15
SalI G'TCGA_C
Cuts at: 2067 2067
Size: 5030
SanDI GG'GwC_CC
Cuts at: 369 369
Size: 5030
SapI GCTCTTCn'nnn_
Cuts at: 214 3053 3263 214
Size: 2839 210 1981
Fragments arranged by size:
2839 1981 210
SbfI CC_TGCA'GG
Cuts at: 2077 2077
Size: 5030
ScaI AGT'ACT
Cuts at: 1537 4547 1537
Size: 3010 2020
SfcI C'TryA_G
Cuts at: 33 260 456 1887 2073 2758 3751 3942 33
Size: 227 196 1431 186 685 993 191 1121
Fragments arranged by size:
1431 1121 993 685 227 196 191 186
SfoI GGC'GCC
Cuts at: 1731 2462 2710 1731
Size: 731 248 4051
Fragments arranged by size:
4051 731 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
SmaI CCC'GGG
Cuts at: 334 2052 334
Size: 1718 3312
SmlI C'TyrA_G
Cuts at: 803 2185 3100 3592 3854 803
Size: 1382 915 492 262 1979
Fragments arranged by size:
1979 1382 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5030
SphI G_CATG'C
Cuts at: 924 2083 2583 924
Size: 1159 500 3371
Fragments arranged by size:
3371 1159 500
StuI AGG'CCT
Cuts at: 167 167
Size: 5030
StyI C'CwwG_G
Cuts at: 107 155 314 950 1277 1385 1526 2164 2518
Size: 48 159 636 327 108 141 638 354
Cuts at: 2518 107
Size: 2619
Fragments arranged by size:
2619 638 636 354 327 159 141 108
48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 657 1588 1686 2613 2746 3205 3414 657
Size: 931 98 927 133 459 209 2273
Fragments arranged by size:
2273 931 927 459 209 133 98
TatI w'GTAC_w
Cuts at: 1043 1535 4545 1043
Size: 492 3010 1528
Fragments arranged by size:
3010 1528 492
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4547 4579 4547
Size: 32 4998
Tth111I GACn'n_nGTC
Cuts at: 2827 2827
Size: 5030
XbaI T'CTAG_A
Cuts at: 2061 2061
Size: 5030
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 645 1392 2525 645
Size: 747 1133 3150
Fragments arranged by size:
3150 1133 747
XmaI C'CCGG_G
Cuts at: 332 2050 332
Size: 1718 3312
XmnI GAAnn'nnTTC
Cuts at: 167 1357 167
Size: 1190 3840
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AlfI AloI AscI AvrII BaeI BcgI
BfrBI BmgBI BmtI BsiWI BsmI BsrGI BstXI BstZ17I
Bsu36I ClaI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NruI NsiI PacI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI