Restriction map for
pF1KE0043
(Circular) (Six-base) MAPSORT of: pF1KE0043.seq Check: 8351 from: 1 to: 4745
pF1KE0043 4745 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1783 1783
Size: 4745
Acc65I G'GTAC_C
Cuts at: 1761 1761
Size: 4745
AclI AA'CG_TT
Cuts at: 4585 4585
Size: 4745
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1493 1533 2570 3002 3749 4017 826
Size: 667 40 1037 432 747 268 1554
Fragments arranged by size:
1554 1037 747 667 432 268 40
AflII C'TTAA_G
Cuts at: 1101 1101
Size: 4745
AflIII A'CryG_T
Cuts at: 236 2097 3201 4178 236
Size: 1861 1104 977 803
Fragments arranged by size:
1861 1104 977 803
AgeI A'CCGG_T
Cuts at: 922 3133 922
Size: 2211 2534
AlwNI CAG_nnn'CTG
Cuts at: 322 540 1425 2050 3617 322
Size: 218 885 625 1567 1450
Fragments arranged by size:
1567 1450 885 625 218
ApaLI G'TGCA_C
Cuts at: 1614 3515 1614
Size: 1901 2844
ApoI r'AATT_y
Cuts at: 479 526 895 993 1531 1543 1601 1749 4098
Size: 47 369 98 538 12 58 148 2349
Cuts at: 4098 4354 479
Size: 256 870
Fragments arranged by size:
2349 870 538 369 256 148 98 58
47 12
AseI AT'TA_AT
Cuts at: 20 1681 20
Size: 1661 3084
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4745
AvaI C'yCGr_G
Cuts at: 360 1765 360
Size: 1405 3340
BamHI G'GATC_C
Cuts at: 1770 1770
Size: 4745
BanI G'GyrC_C
Cuts at: 1761 2175 2423 2458 1761
Size: 414 248 35 4048
Fragments arranged by size:
4048 414 248 35
BanII G_rGCy'C
Cuts at: 420 1759 2789 420
Size: 1339 1030 2376
Fragments arranged by size:
2376 1339 1030
BbeI G_GCGC'C
Cuts at: 2179 2427 2179
Size: 248 4497
BbsI GAAGACnn'nnnn_
Cuts at: 166 279 439 965 1431 166
Size: 113 160 526 466 3480
Fragments arranged by size:
3480 526 466 160 113
BbvCI CC'TCA_GC
Cuts at: 712 712
Size: 4745
BciVI GTATCCnnnnn_n'
Cuts at: 2643 3404 4745 2643
Size: 761 1341 2643
Fragments arranged by size:
2643 1341 761
BclI T'GATC_A
Cuts at: 1712 2265 1712
Size: 553 4192
BglII A'GATC_T
Cuts at: 170 1665 2260 4297 170
Size: 1495 595 2037 618
Fragments arranged by size:
2037 1495 618 595
BlpI GC'TnA_GC
Cuts at: 1857 1857
Size: 4745
Bme1580I G_kGCm'C
Cuts at: 182 698 852 1618 2370 2463 3519 182
Size: 516 154 766 752 93 1056 1408
Fragments arranged by size:
1408 1056 766 752 516 154 93
BmgBI CAC'GTC
Cuts at: 237 237
Size: 4745
BmrI ACTGGGnnnn_n'
Cuts at: 451 738 1013 1957 2038 2373 4517 451
Size: 287 275 944 81 335 2144 679
Fragments arranged by size:
2144 944 679 335 287 275 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3112 3112
Size: 4745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 1672 187
Size: 1485 3260
Bpu10I CC'TnA_GC
Cuts at: 712 712
Size: 4745
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1921 2800 3292 3590 1921
Size: 879 492 298 3076
Fragments arranged by size:
3076 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4449 4449
Size: 4745
BsaAI yAC'GTr
Cuts at: 2098 2098
Size: 4745
BsaBI GATnn'nnATC
Cuts at: 6 2259 4296 6
Size: 2253 2037 455
Fragments arranged by size:
2253 2037 455
BsaHI Gr'CG_yC
Cuts at: 2176 2424 3126 4622 2176
Size: 248 702 1496 2299
Fragments arranged by size:
2299 1496 702 248
BsaWI w'CCGG_w
Cuts at: 922 1934 2455 3133 3407 3554 3965 922
Size: 1012 521 678 274 147 411 1702
Fragments arranged by size:
1702 1012 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1028 1058 1028
Size: 30 4715
BseYI C'CCAG_C
Cuts at: 548 2170 2928 3505 4001 548
Size: 1622 758 577 496 1292
Fragments arranged by size:
1622 1292 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2287 2333 3541 104
Size: 2183 46 1208 1308
Fragments arranged by size:
2183 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1618 1759 2537 2727 3519 1618
Size: 141 778 190 792 2844
Fragments arranged by size:
2844 792 778 190 141
Bsp1286I G_dGCh'C
Cuts at: 182 420 698 852 1618 1759 2370 2463 2537
Size: 238 278 154 766 141 611 93 74
Cuts at: 2537 2727 2789 3519 182
Size: 190 62 730 1408
Fragments arranged by size:
1408 766 730 611 278 238 190 154
141 93 74 62
BspEI T'CCGG_A
Cuts at: 3965 3965
Size: 4745
BspHI T'CATG_A
Cuts at: 3921 3921
Size: 4745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1795 2009 2311 2692 1795
Size: 214 302 381 3848
Fragments arranged by size:
3848 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3037 3091 4575 4740 50
Size: 2987 54 1484 165 55
Fragments arranged by size:
2987 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2657 2657
Size: 4745
BsrFI r'CCGG_y
Cuts at: 922 2743 2924 3133 922
Size: 1821 181 209 2534
Fragments arranged by size:
2534 1821 209 181
BssSI C'ACGA_G
Cuts at: 691 3016 3374 691
Size: 2325 358 2062
Fragments arranged by size:
2325 2062 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2122 2122
Size: 4745
BstBI TT'CG_AA
Cuts at: 3106 3106
Size: 4745
BstXI CCAn_nnnn'nTGG
Cuts at: 408 408
Size: 4745
BstYI r'GATC_y
Cuts at: 1 170 599 767 1665 1770 2237 2260 2595
Size: 169 429 168 898 105 467 23 335
Cuts at: 2595 2841 3853 3939 3951 4297 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 898 467 449 429 346 335 246
169 168 105 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 427 427
Size: 4745
BtgI C'CryG_G
Cuts at: 107 401 107
Size: 294 4451
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2695 2876 3039 2695
Size: 181 163 4401
Fragments arranged by size:
4401 181 163
BtsI GCAGTG_nn'
Cuts at: 725 1988 725
Size: 1263 3482
DraI TTT'AAA
Cuts at: 1744 1744
Size: 4745
DraIII CAC_nnn'GTG
Cuts at: 1267 1267
Size: 4745
DrdI GACnn_nn'nnGTC
Cuts at: 2451 3309 2451
Size: 858 3887
EaeI y'GGCC_r
Cuts at: 2330 2504 2895 2922 4267 2330
Size: 174 391 27 1345 2808
Fragments arranged by size:
2808 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2330 2330
Size: 4745
EarI CTCTTCn'nnn_
Cuts at: 342 673 1574 2768 2978 342
Size: 331 901 1194 210 2109
Fragments arranged by size:
2109 1194 901 331 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2953 3263 3409 4555 2953
Size: 310 146 1146 3143
Fragments arranged by size:
3143 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1757 1757
Size: 4745
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 826 1493 1533 1672 2570 3002 3749 4017
Size: 639 667 40 139 898 432 747 268
Cuts at: 4017 187
Size: 915
Fragments arranged by size:
915 898 747 667 639 432 268 139
40
EcoO109I rG'GnC_Cy
Cuts at: 357 1422 1884 357
Size: 1065 462 3218
Fragments arranged by size:
3218 1065 462
EcoRI G'AATT_C
Cuts at: 479 1543 1749 479
Size: 1064 206 3475
Fragments arranged by size:
3475 1064 206
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 471 2764 471
Size: 2293 2452
FspI TGC'GCA
Cuts at: 2526 2526
Size: 4745
HaeII r_GCGC'y
Cuts at: 227 2179 2427 3449 4428 227
Size: 1952 248 1022 979 544
Fragments arranged by size:
1952 1022 979 544 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 319 351 1261 1293 2587 2617 2619 2649 319
Size: 32 910 32 1294 30 2 30 2415
Fragments arranged by size:
2415 1294 910 32 32 30 30 2
HincII GTy'rAC
Cuts at: 663 1784 663
Size: 1121 3624
HindIII A'AGCT_T
Cuts at: 115 905 1138 115
Size: 790 233 3722
Fragments arranged by size:
3722 790 233
KasI G'GCGC_C
Cuts at: 2175 2423 2175
Size: 248 4497
KpnI G_GTAC'C
Cuts at: 1765 1765
Size: 4745
MluI A'CGCG_T
Cuts at: 4178 4178
Size: 4745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 803 3416 3600 803
Size: 2613 184 1948
Fragments arranged by size:
2613 1948 184
MscI TGG'CCA
Cuts at: 2506 2506
Size: 4745
MslI CAynn'nnrTG
Cuts at: 406 469 2734 2861 406
Size: 63 2265 127 2290
Fragments arranged by size:
2290 2265 127 63
MspA1I CmG'CkG
Cuts at: 322 764 1856 2170 2530 3543 3788 322
Size: 442 1092 314 360 1013 245 1279
Fragments arranged by size:
1279 1092 1013 442 360 314 245
NaeI GCC'GGC
Cuts at: 2926 2926
Size: 4745
NarI GG'CG_CC
Cuts at: 2176 2424 2176
Size: 248 4497
NcoI C'CATG_G
Cuts at: 107 401 107
Size: 294 4451
NgoMIV G'CCGG_C
Cuts at: 2924 2924
Size: 4745
NspI r_CATG'y
Cuts at: 1798 2298 3205 1798
Size: 500 907 3338
Fragments arranged by size:
3338 907 500
PciI A'CATG_T
Cuts at: 3201 3201
Size: 4745
PflMI CCAn_nnn'nTGG
Cuts at: 540 540
Size: 4745
PfoI T'CCnGG_A
Cuts at: 1046 1046
Size: 4745
PmeI GTTT'AAAC
Cuts at: 1744 1744
Size: 4745
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 3884
Size: 32 4713
PpuMI rG'GwC_Cy
Cuts at: 357 357
Size: 4745
PstI C_TGCA'G
Cuts at: 327 1792 2477 327
Size: 1465 685 2595
Fragments arranged by size:
2595 1465 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4745
PvuII CAG'CTG
Cuts at: 322 764 2170 2530 322
Size: 442 1406 360 2537
Fragments arranged by size:
2537 1406 442 360
RsrII CG'GwC_CG
Cuts at: 2940 2940
Size: 4745
SacI G_AGCT'C
Cuts at: 1759 1759
Size: 4745
SalI G'TCGA_C
Cuts at: 1782 1782
Size: 4745
SapI GCTCTTCn'nnn_
Cuts at: 1574 2768 2978 1574
Size: 1194 210 3341
Fragments arranged by size:
3341 1194 210
SbfI CC_TGCA'GG
Cuts at: 1792 1792
Size: 4745
ScaI AGT'ACT
Cuts at: 4262 4262
Size: 4745
SexAI A'CCwGG_T
Cuts at: 353 507 539 353
Size: 154 32 4559
Fragments arranged by size:
4559 154 32
SfcI C'TryA_G
Cuts at: 33 323 1788 2473 3466 3657 33
Size: 290 1465 685 993 191 1121
Fragments arranged by size:
1465 1121 993 685 290 191
SfoI GGC'GCC
Cuts at: 2177 2425 2177
Size: 248 4497
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4745
SgrAI Cr'CCGG_yG
Cuts at: 922 922
Size: 4745
SmaI CCC'GGG
Cuts at: 362 1767 362
Size: 1405 3340
SmlI C'TyrA_G
Cuts at: 1101 1900 2815 3307 3569 1101
Size: 799 915 492 262 2277
Fragments arranged by size:
2277 915 799 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4745
SphI G_CATG'C
Cuts at: 1798 2298 1798
Size: 500 4245
SspI AAT'ATT
Cuts at: 1161 1685 1161
Size: 524 4221
StyI C'CwwG_G
Cuts at: 107 401 678 1879 2233 107
Size: 294 277 1201 354 2619
Fragments arranged by size:
2619 1201 354 294 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 428 720 1032 1099 2328 2461 2920 3129 428
Size: 292 312 67 1229 133 459 209 2044
Fragments arranged by size:
2044 1229 459 312 292 209 133 67
TatI w'GTAC_w
Cuts at: 243 438 4260 243
Size: 195 3822 728
Fragments arranged by size:
3822 728 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 389 421 718 750 4262 4294 389
Size: 32 297 32 3512 32 840
Fragments arranged by size:
3512 840 297 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 355 1450 2542 355
Size: 1095 1092 2558
Fragments arranged by size:
2558 1095 1092
XbaI T'CTAG_A
Cuts at: 1776 1776
Size: 4745
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 604 2240 604
Size: 1636 3109
XmaI C'CCGG_G
Cuts at: 360 1765 360
Size: 1405 3340
XmnI GAAnn'nnTTC
Cuts at: 116 1583 116
Size: 1467 3278
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SnaBI SrfI StuI SwaI XhoI ZraI