Restriction map for
pF1KE0038
(Circular) (Six-base) MAPSORT of: pF1KE0038.seq Check: 2147 from: 1 to: 5219
pF1KE0038 5219 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1979 2257 1979
Size: 278 4941
Acc65I G'GTAC_C
Cuts at: 1762 2235 1762
Size: 473 4746
AclI AA'CG_TT
Cuts at: 2213 5059 2213
Size: 2846 2373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 3044 3476 4223 4491 137
Size: 2907 432 747 268 865
Fragments arranged by size:
2907 865 747 432 268
AflIII A'CryG_T
Cuts at: 614 1900 2176 2571 3675 4652 614
Size: 1286 276 395 1104 977 1181
Fragments arranged by size:
1286 1181 1104 977 395 276
AgeI A'CCGG_T
Cuts at: 3607 3607
Size: 5219
AleI CACnn'nnGTG
Cuts at: 615 1670 615
Size: 1055 4164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2216 2216
Size: 5219
AlwNI CAG_nnn'CTG
Cuts at: 2524 4091 2524
Size: 1567 3652
ApaLI G'TGCA_C
Cuts at: 3989 3989
Size: 5219
ApoI r'AATT_y
Cuts at: 254 336 507 980 1303 1333 1705 1859 2056
Size: 82 171 473 323 30 372 154 197
Cuts at: 2056 2122 2223 4572 4828 254
Size: 66 101 2349 256 645
Fragments arranged by size:
2349 645 473 372 323 256 197 171
154 101 82 66 30
AseI AT'TA_AT
Cuts at: 20 494 539 20
Size: 474 45 4700
Fragments arranged by size:
4700 474 45
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5219
AvaI C'yCGr_G
Cuts at: 2239 2239
Size: 5219
BamHI G'GATC_C
Cuts at: 2244 2244
Size: 5219
BanI G'GyrC_C
Cuts at: 1762 2235 2649 2897 2932 1762
Size: 473 414 248 35 4049
Fragments arranged by size:
4049 473 414 248 35
BanII G_rGCy'C
Cuts at: 1887 2233 3263 1887
Size: 346 1030 3843
Fragments arranged by size:
3843 1030 346
BbeI G_GCGC'C
Cuts at: 2653 2901 2653
Size: 248 4971
BbsI GAAGACnn'nnnn_
Cuts at: 621 621
Size: 5219
BciVI GTATCCnnnnn_n'
Cuts at: 3117 3878 5219 3117
Size: 761 1341 3117
Fragments arranged by size:
3117 1341 761
BclI T'GATC_A
Cuts at: 2739 2739
Size: 5219
BglII A'GATC_T
Cuts at: 828 2734 4771 828
Size: 1906 2037 1276
Fragments arranged by size:
2037 1906 1276
BlpI GC'TnA_GC
Cuts at: 2331 2331
Size: 5219
Bme1580I G_kGCm'C
Cuts at: 2844 2937 3993 2844
Size: 93 1056 4070
Fragments arranged by size:
4070 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 793 2431 2512 2847 4991 793
Size: 1638 81 335 2144 1021
Fragments arranged by size:
2144 1638 1021 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3586 3586
Size: 5219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1627 1627
Size: 5219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1566 2395 3274 3766 4064 1566
Size: 829 879 492 298 2721
Fragments arranged by size:
2721 879 829 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1645 4923 1645
Size: 3278 1941
BsaAI yAC'GTr
Cuts at: 810 2572 810
Size: 1762 3457
BsaBI GATnn'nnATC
Cuts at: 6 1022 1575 2733 4770 6
Size: 1016 553 1158 2037 455
Fragments arranged by size:
2037 1158 1016 553 455
BsaHI Gr'CG_yC
Cuts at: 2650 2898 3600 5096 2650
Size: 248 702 1496 2773
Fragments arranged by size:
2773 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2408 2929 3607 3881 4028 4439 2408
Size: 521 678 274 147 411 3188
Fragments arranged by size:
3188 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1189 1219 1189
Size: 30 5189
BseYI C'CCAG_C
Cuts at: 2010 2644 3402 3979 4475 2010
Size: 634 758 577 496 2754
Fragments arranged by size:
2754 758 634 577 496
BsiEI CG_ry'CG
Cuts at: 104 2761 2807 4015 104
Size: 2657 46 1208 1308
Fragments arranged by size:
2657 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 838 1935 2233 3011 3201 3993 838
Size: 1097 298 778 190 792 2064
Fragments arranged by size:
2064 1097 792 778 298 190
BsmI GAATG_Cn'
Cuts at: 750 1681 750
Size: 931 4288
Bsp1286I G_dGCh'C
Cuts at: 838 1887 1935 2233 2844 2937 3011 3201 3263
Size: 1049 48 298 611 93 74 190 62
Cuts at: 3263 3993 838
Size: 730 2064
Fragments arranged by size:
2064 1049 730 611 298 190 93 74
62 48
BspEI T'CCGG_A
Cuts at: 4439 4439
Size: 5219
BspHI T'CATG_A
Cuts at: 4395 4395
Size: 5219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2269 2483 2785 3166 2269
Size: 214 302 381 4322
Fragments arranged by size:
4322 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3511 3565 5049 5214 50
Size: 3461 54 1484 165 55
Fragments arranged by size:
3461 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 186 991 1826 3131 186
Size: 805 835 1305 2274
Fragments arranged by size:
2274 1305 835 805
BsrFI r'CCGG_y
Cuts at: 1414 3217 3398 3607 1414
Size: 1803 181 209 3026
Fragments arranged by size:
3026 1803 209 181
BsrGI T'GTAC_A
Cuts at: 1696 1696
Size: 5219
BssSI C'ACGA_G
Cuts at: 3490 3848 3490
Size: 358 4861
BstAPI GCAn_nnn'nTGC
Cuts at: 1225 2596 1225
Size: 1371 3848
BstBI TT'CG_AA
Cuts at: 3580 3580
Size: 5219
BstYI r'GATC_y
Cuts at: 1 370 620 787 828 2244 2711 2734 3069
Size: 369 250 167 41 1416 467 23 335
Cuts at: 3069 3315 4327 4413 4425 4771 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1416 1012 467 449 369 346 335 250
246 167 86 41 23 12
Bsu36I CC'TnA_GG
Cuts at: 854 854
Size: 5219
BtgI C'CryG_G
Cuts at: 107 107
Size: 5219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3169 3350 3513 3169
Size: 181 163 4875
Fragments arranged by size:
4875 181 163
BtsI GCAGTG_nn'
Cuts at: 2462 2462
Size: 5219
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1908 1943 1908
Size: 35 5184
DraI TTT'AAA
Cuts at: 1188 2218 1188
Size: 1030 4189
DraIII CAC_nnn'GTG
Cuts at: 238 238
Size: 5219
DrdI GACnn_nn'nnGTC
Cuts at: 2925 3783 2925
Size: 858 4361
EaeI y'GGCC_r
Cuts at: 2804 2978 3369 3396 4741 2804
Size: 174 391 27 1345 3282
Fragments arranged by size:
3282 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2804 2804
Size: 5219
EarI CTCTTCn'nnn_
Cuts at: 300 3242 3452 300
Size: 2942 210 2067
Fragments arranged by size:
2942 2067 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3427 3737 3883 5029 3427
Size: 310 146 1146 3617
Fragments arranged by size:
3617 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2231 2231
Size: 5219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 1627 3044 3476 4223 4491 137
Size: 1490 1417 432 747 268 865
Fragments arranged by size:
1490 1417 865 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 2358 2358
Size: 5219
EcoRI G'AATT_C
Cuts at: 1859 2122 2223 1859
Size: 263 101 4855
Fragments arranged by size:
4855 263 101
EcoRV GAT'ATC
Cuts at: 1358 1358
Size: 5219
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 213 3238 213
Size: 3025 2194
FspI TGC'GCA
Cuts at: 3000 3000
Size: 5219
HaeII r_GCGC'y
Cuts at: 2653 2901 3923 4902 2653
Size: 248 1022 979 2970
Fragments arranged by size:
2970 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 277 309 1277 1309 3061 3091 3093
Size: 32 112 32 968 32 1752 30 2
Cuts at: 3093 3123 133
Size: 30 2229
Fragments arranged by size:
2229 1752 968 112 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1781 2258 1781
Size: 477 4742
HindIII A'AGCT_T
Cuts at: 321 321
Size: 5219
KasI G'GCGC_C
Cuts at: 2649 2897 2649
Size: 248 4971
KpnI G_GTAC'C
Cuts at: 1766 2239 1766
Size: 473 4746
MfeI C'AATT_G
Cuts at: 1497 1497
Size: 5219
MluI A'CGCG_T
Cuts at: 4652 4652
Size: 5219
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 852 1302 3890 4074 852
Size: 450 2588 184 1997
Fragments arranged by size:
2588 1997 450 184
MscI TGG'CCA
Cuts at: 2980 2980
Size: 5219
MslI CAynn'nnrTG
Cuts at: 615 1463 1670 3208 3335 615
Size: 848 207 1538 127 2499
Fragments arranged by size:
2499 1538 848 207 127
MspA1I CmG'CkG
Cuts at: 1925 2330 2644 3004 4017 4262 1925
Size: 405 314 360 1013 245 2882
Fragments arranged by size:
2882 1013 405 360 314 245
NaeI GCC'GGC
Cuts at: 1416 3400 1416
Size: 1984 3235
NarI GG'CG_CC
Cuts at: 2650 2898 2650
Size: 248 4971
NcoI C'CATG_G
Cuts at: 107 107
Size: 5219
NgoMIV G'CCGG_C
Cuts at: 1414 3398 1414
Size: 1984 3235
NspI r_CATG'y
Cuts at: 618 1000 1904 2180 2193 2272 2772 3679 618
Size: 382 904 276 13 79 500 907 2158
Fragments arranged by size:
2158 907 904 500 382 276 79 13
PacI TTA_AT'TAA
Cuts at: 539 539
Size: 5219
PciI A'CATG_T
Cuts at: 614 1900 2176 3675 614
Size: 1286 276 1499 2158
Fragments arranged by size:
2158 1499 1286 276
PflMI CCAn_nnn'nTGG
Cuts at: 772 1528 1836 772
Size: 756 308 4155
Fragments arranged by size:
4155 756 308
PmeI GTTT'AAAC
Cuts at: 2218 2218
Size: 5219
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4358 4390 4358
Size: 32 5187
PsiI TTA'TAA
Cuts at: 1613 1843 1613
Size: 230 4989
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1639 1671 2132 2164 1639
Size: 32 461 32 4694
Fragments arranged by size:
4694 461 32 32
PstI C_TGCA'G
Cuts at: 2266 2951 2266
Size: 685 4534
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5219
PvuII CAG'CTG
Cuts at: 1925 2644 3004 1925
Size: 719 360 4140
Fragments arranged by size:
4140 719 360
RsrII CG'GwC_CG
Cuts at: 3414 3414
Size: 5219
SacI G_AGCT'C
Cuts at: 2233 2233
Size: 5219
SalI G'TCGA_C
Cuts at: 2256 2256
Size: 5219
SapI GCTCTTCn'nnn_
Cuts at: 3242 3452 3242
Size: 210 5009
SbfI CC_TGCA'GG
Cuts at: 2266 2266
Size: 5219
ScaI AGT'ACT
Cuts at: 4736 4736
Size: 5219
SfcI C'TryA_G
Cuts at: 33 1617 1994 2262 2947 3940 4131 33
Size: 1584 377 268 685 993 191 1121
Fragments arranged by size:
1584 1121 993 685 377 268 191
SfoI GGC'GCC
Cuts at: 2651 2899 2651
Size: 248 4971
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5219
SmaI CCC'GGG
Cuts at: 2241 2241
Size: 5219
SmlI C'TyrA_G
Cuts at: 1581 2374 3289 3781 4043 1581
Size: 793 915 492 262 2757
Fragments arranged by size:
2757 915 793 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5219
SphI G_CATG'C
Cuts at: 2272 2772 2272
Size: 500 4719
StyI C'CwwG_G
Cuts at: 107 1047 2075 2353 2707 107
Size: 940 1028 278 354 2619
Fragments arranged by size:
2619 1028 940 354 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 402 1660 2802 2935 3394 3603 402
Size: 1258 1142 133 459 209 2018
Fragments arranged by size:
2018 1258 1142 459 209 133
TatI w'GTAC_w
Cuts at: 1696 4734 1696
Size: 3038 2181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4736 4768 4736
Size: 32 5187
Tth111I GACn'n_nGTC
Cuts at: 3016 3016
Size: 5219
XbaI T'CTAG_A
Cuts at: 2250 2250
Size: 5219
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 675 2714 675
Size: 2039 3180
XmaI C'CCGG_G
Cuts at: 2239 2239
Size: 5219
XmnI GAAnn'nnTTC
Cuts at: 981 1443 1708 981
Size: 462 265 4492
Fragments arranged by size:
4492 462 265
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PacI PciI
PflMI PmeI PpiI PsiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I ClaI EcoNI FseI FspAI HpaI NdeI NheI
NotI NruI NsiI PasI PfoI PmlI PpuMI PshAI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI